Affinity labeling of gamma-glutamyl transpeptidase and location of the gamma-glutamyl binding site on the light subunit.

abstract：:Sponges (Porifera), the simplest and earliest multicellular organisms, are thought to have evolved from their unicellular ancestors about 1 billion years ago by developing cell-recognition and adhesion mechanisms to discriminate against "non-self." Consequently, they are used as models for investigating recognition ph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haseley SR,Vermeer HJ,Kamerling JP,Vliegenthart JF

更新日期：2001-07-31 00:00:00

abstract：:Rat, but not human, erythrocytes undergo fusion promoted by the membrane-mobility agent 2-(2-methoxyethoxy)-ethyl cis-8-(2-octylcyclopropyl)octanoate (A2C). The difference in behavior is correlated with rat erythrocyte membrane protein degradation caused by Ca2+-activated proteases. The human erythrocyte is deficient ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kosower NS,Glaser T,Kosower EM

更新日期：1983-12-01 00:00:00

abstract：:The 2012-2015 drought has left California with severely reduced snowpack, soil moisture, ground water, and reservoir stocks, but the impact of this estimated millennial-scale event on forest health is unknown. We used airborne laser-guided spectroscopy and satellite-based models to assess losses in canopy water conten...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Asner GP,Brodrick PG,Anderson CB,Vaughn N,Knapp DE,Martin RE

更新日期：2016-01-12 00:00:00

abstract：:Current evidence suggests that the length of poly(A) tails of bacterial mRNAs result from a competition between poly(A) polymerase and exoribonucleases that attack the 3' ends of RNAs. Here, we show that host factor Hfq is also involved in poly(A) tail metabolism. Inactivation of the hfq gene reduces the length of pol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hajnsdorf E,Régnier P

更新日期：2000-02-15 00:00:00

abstract：:Using [3H]quinuclidinyl benzilate as a specific cholinergic muscarinic ligand, it has been demonstrated that lymphocytes have muscarinic binding sites. There are approximately 200 sites per cell and the dissociation constant for quinuclidinyl benzilate is approximately 1 X 10(-9) M. Quinuclidinyl benzilate receptor bi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gordon MA,Cohen JJ,Wilson IB

更新日期：1978-06-01 00:00:00

abstract：:Four polyimidazoles were used in the binding and cleavage studies with poly(U). The two polydisperse polyvinylimidazoles were previously described by others, while the other two new polymers of polyethyleneimines were prepared by cationic polymerization of oxazolines. The latter had imidazole units attached to each ni...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheng L,Abhilash KG,Breslow R

更新日期：2012-08-07 00:00:00

abstract：:A cDNA expression library in lambda gt11 was screened with affinity-purified polyclonal anti-rat cytochrome b5 reductase antibodies. One positive clone out of 450,000 clones was isolated and found to be incomplete. This clone was used to rescreen the library, and a second, overlapping clone that contained the entire c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pietrini G,Carrera P,Borgese N

更新日期：1988-10-01 00:00:00

abstract：:New neurons are generated in adult mammalians and may contribute to repairing the brain after injury. Here, we show that the number of new neurons in the dentate gyrus of adult rats increased in cerebral ischemic stroke and correlated with activation of the cAMP-response-element-binding protein (CREB). Inhibition of e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu DY,Lau L,Liu SH,Wei JS,Lu YM

更新日期：2004-06-22 00:00:00

abstract：:RNase T2 enzymes are conserved in most eukaryotic genomes, and expression patterns and phylogenetic analyses suggest that they may carry out an important housekeeping role. However, the nature of this role has been elusive. Here we show that RNS2, an intracellular RNase T2 from Arabidopsis thaliana, is essential for n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hillwig MS,Contento AL,Meyer A,Ebany D,Bassham DC,Macintosh GC

更新日期：2011-01-18 00:00:00

abstract：:The class II transactivator (CIITA) is the master integrator of expression of MHC class II genes. It interacts with variety of basal transcription factors to initiate and elongate transcription of these genes. Among others, it recruits positive transcription elongation factor b (P-TEFb) to MHC class II promoters. In c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kohoutek J,Blazek D,Peterlin BM

更新日期：2006-11-14 00:00:00

abstract：:The substrate specificity of thermophilic xylose isomerase from Clostridium thermosulfurogenes was examined by using predictions from the known crystal structure of the Arthrobacter enzyme and site-directed mutagenesis of the thermophile xylA gene. The orientation of glucose as a substrate in the active site of the th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meng M,Lee C,Bagdasarian M,Zeikus JG

更新日期：1991-05-01 00:00:00

abstract：:Methods for the construction of ordered nanoscale arrays have been implicated in fields ranging from separation technologies to microelectronics. Yet, despite the plethora of nanoscale structures assembled in nature that use a templating strategy, chemists have been unable to replicate this success. A technology is re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Willis B,Eubanks LM,Wood MR,Janda KD,Dickerson TJ,Lerner RA

更新日期：2008-02-05 00:00:00

abstract：:The activity of cullin-containing ubiquitin protein ligase complexes is stimulated by linkage to cullin of the ubiquitin-like protein Nedd8 ("neddylation"). Neddylation is inhibited by the tight binding of cullins to CAND1 (cullin-associated and neddylation-dissociated 1) protein, and Nedd8 is removed from cullins by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bornstein G,Ganoth D,Hershko A

更新日期：2006-08-01 00:00:00

abstract：:We have reexamined the balance between cell birth, cell maturation, and cell death in the thymus by labeling dividing thymocytes and their progeny in vivo with [3H]-thymidine, isolating clearly defined subpopulations by fluorescence-activated cell sorting, and determining the distribution of label by autoradiography. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Egerton M,Scollay R,Shortman K

更新日期：1990-04-01 00:00:00

abstract：:Obesity is a major public health problem and is often associated with type 2 diabetes mellitus, cardiovascular disease, and metabolic syndrome. Leptin is the crucial adipostatic hormone that controls food intake and body weight through the activation of specific leptin receptors (OB-R) in the hypothalamic arcuate nucl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Couturier C,Sarkis C,Séron K,Belouzard S,Chen P,Lenain A,Corset L,Dam J,Vauthier V,Dubart A,Mallet J,Froguel P,Rouillé Y,Jockers R

更新日期：2007-12-04 00:00:00

abstract：:In the mammalian central nervous system, the excitatory amino acid transmitter L-glutamate activates three pharmacologically distinguishable receptors, the N-methyl-D-aspartate (NMDA), kainate, and quisqualate receptors. The present paper addresses the issue of whether these three receptors operate independent channel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lerma J,Kushner L,Zukin RS,Bennett MV

更新日期：1989-03-01 00:00:00

abstract：:For decades, scientists have debated whether supercooled liquids stop flowing below a glass transition temperature [Formula: see text] or whether motion continues to slow gradually down to zero temperature. Answering this question is challenging because human time scales set a limit on the largest measurable viscosity...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jadhao V,Robbins MO

更新日期：2017-07-25 00:00:00

abstract：:The feeling of being observed or merely participating in an experiment can affect individuals' behavior. Referred to as the Hawthorne effect, this inconsistently observed phenomenon can both provide insight into individuals' behavior and confound the interpretation of experimental manipulations. Here, we pursue both t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwartz D,Fischhoff B,Krishnamurti T,Sowell F

更新日期：2013-09-17 00:00:00

abstract：:The attachment of human polymorphonuclear leukocytes (PMN) to bovine endothelial monolayers is increased by pretreatment of the endothelial cells with leukotriene B4 (LTB4). The response is dose dependent with a maximum at 10(-6) M, requires no more than 1 min of preincubation for the maximal effect, and decays linear...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoover RL,Karnovsky MJ,Austen KF,Corey EJ,Lewis RA

更新日期：1984-04-01 00:00:00

abstract：:A glycoprotein was isolated and purified to homogeneity from the serum of a patient with chronic non-A, non-B hepatitis. NaDodSO4/PAGE of the glycoprotein revealed a single major band at Mr approximately 77,000. Antibodies to this glycoprotein were shown to possess the following immunoreactivity: (i) they reacted by r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seto B,Gerety RJ

更新日期：1985-08-01 00:00:00

abstract：:Many "workers" in north temperate colonies of the eusocial paper wasp Polistes fuscatus disappear within a few days of eclosion. We provide evidence that these females are pursuing an alternative reproductive strategy, i.e., dispersing to overwinter and become nest foundresses the following spring, instead of helping ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reeve HK,Peters JM,Nonacs P,Starks PT

更新日期：1998-11-10 00:00:00

abstract：:The endbrain (telencephalon) is at the rostral end of the central nervous system and is primarily responsible for supporting cognition and affect. Structurally, it consists of right and left cerebral hemispheres, each parceled into multiple cortical and nuclear gray matter regions. The global network organization of a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Swanson LW,Hahn JD,Jeub LGS,Fortunato S,Sporns O

更新日期：2018-07-17 00:00:00

abstract：:In multiple binding of ligands to a protein, the binding sites may seem to behave as if they are partitioned equally between two modalities. This paper analyzes three different molecular situations in which two actual assemblages appear: (i) two classes of sites exist at the outset in the ligand-free macromolecule; (i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klotz IM,Hunston DL

更新日期：1977-11-01 00:00:00

abstract：:We have examined the process by which small groups of pigmented germinal cells transplanted orthotopically from stage 30-38 donor embryos into stage 28-38 albino hosts contribute new postmitotic cells to the pigmented retinal epithelium of the growing larval eye in Xenopus. In the great majority of chimeric eyes, the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hunt RK,Cohen JS,Mason BJ

更新日期：1987-05-01 00:00:00

abstract：:Historical collapse of ancient states or civilizations has raised new awareness about its possible relevance to current issues of sustainability, in the context of global change. This Special Feature examines 12 case studies of societies under stress, of which seven suffered severe transformation. Outcomes were comple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butzer KW,Endfield GH

更新日期：2012-03-06 00:00:00

abstract：:Proteasomes are the multi-subunit protease thought to play a key role in the generation of peptides presented by major histocompatibility complex (MHC) class I molecules. When cells are stimulated with interferon gamma, two MHC-encoded subunits, low molecular mass polypeptide (LMP) 2 and LMP7, and the MECL1 subunit en...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kasahara M,Hayashi M,Tanaka K,Inoko H,Sugaya K,Ikemura T,Ishibashi T

更新日期：1996-08-20 00:00:00

abstract：:In recent years, advertisers and magazine editors have been widely criticized for taking digital photo retouching to an extreme. Impossibly thin, tall, and wrinkle- and blemish-free models are routinely splashed onto billboards, advertisements, and magazine covers. The ubiquity of these unrealistic and highly idealize...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kee E,Farid H

更新日期：2011-12-13 00:00:00

abstract：:A typing method for bacteria was developed and applied to several species, including Escherichia coli and Actinobacillus actinomycetemcomitans. Total genomic DNA was digested with a restriction endonuclease, and fragments were enabled with [alpha-32P]dATP by using the Klenow fragment of DNA polymerase and separated by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van Steenbergen TJ,Colloms SD,Hermans PW,de Graaff J,Plasterk RH

更新日期：1995-06-06 00:00:00

abstract：:Repetitive brain stimulation protocols induce plasticity in the stimulated site in brain slice models. Recent evidence from network models has indicated that additional plasticity-related changes occur in nonstimulated remote regions. Despite increasing use of brain stimulation protocols in experimental and clinical s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Abe M,Fukuyama H,Mima T

更新日期：2014-03-25 00:00:00

abstract：:Dengue virus (DENV) is a global disease threat for which there are no approved antivirals or vaccines. Establishing state-of-the-art screening systems that rely on fluorescent or luminescent reporters may accelerate the development of anti-DENV therapeutics. However, relatively few reporter DENV platforms exist. Here,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schoggins JW,Dorner M,Feulner M,Imanaka N,Murphy MY,Ploss A,Rice CM

更新日期：2012-09-04 00:00:00