Hexim1 sequesters positive transcription elongation factor b from the class II transactivator on MHC class II promoters.

Abstract:

:The class II transactivator (CIITA) is the master integrator of expression of MHC class II genes. It interacts with variety of basal transcription factors to initiate and elongate transcription of these genes. Among others, it recruits positive transcription elongation factor b (P-TEFb) to MHC class II promoters. In cells, P-TEFb is found in small active or large inactive complexes. The large complex is composed of P-TEFb, 7SK small nuclear RNA, and hexamethylene bisacetamide-inducible protein 1 (Hexim1). The present study identifies Hexim1 as a potent inhibitor of CIITA-mediated transcription. Not only the exogenously expressed but also IFN-gamma-induced CIITA was inhibited by Hexim1. This inhibition did not result from an association between Hexim1 and CIITA but depended on the intact Cyclin T1-binding domain in Hexim1. Importantly, Hexim1 sequestered P-TEFb from CIITA, as documented by binding competition and ChIP assays. Conversely, the depletion of Hexim1 from cells by siRNA increased CIITA-mediated transcription. Thus, modulating ratios between active and inactive P-TEFb complexes is an additional mechanism of regulating transcriptional activators such as CIITA.

authors

Kohoutek J,Blazek D,Peterlin BM

doi

10.1073/pnas.0603079103

subject

Has Abstract

pub_date

2006-11-14 00:00:00

pages

17349-54

issue

46

eissn

0027-8424

issn

1091-6490

pii

0603079103

journal_volume

103

pub_type

杂志文章
  • A subset of group A-like var genes encodes the malaria parasite ligands for binding to human brain endothelial cells.

    abstract::Cerebral malaria is the most deadly manifestation of infection with Plasmodium falciparum. The pathology of cerebral malaria is characterized by the accumulation of infected erythrocytes (IEs) in the microvasculature of the brain caused by parasite adhesins on the surface of IEs binding to human receptors on microvasc...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1120461109

    authors: Claessens A,Adams Y,Ghumra A,Lindergard G,Buchan CC,Andisi C,Bull PC,Mok S,Gupta AP,Wang CW,Turner L,Arman M,Raza A,Bozdech Z,Rowe JA

    更新日期:2012-06-26 00:00:00

  • Sleep forms memory for finger skills.

    abstract::Practicing a motor skill triggers a process of memory consolidation that continues for hours after practice has ended, and becomes manifest in an improved skill at later testing. We used a sequential motor task (finger-to-thumb opposition task) to show that, in humans, the formation of motor skill memories essentially...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.182178199

    authors: Fischer S,Hallschmid M,Elsner AL,Born J

    更新日期:2002-09-03 00:00:00

  • Structural basis of nonnatural amino acid recognition by an engineered aminoacyl-tRNA synthetase for genetic code expansion.

    abstract::The genetic code in a eukaryotic system has been expanded by the engineering of Escherichia coli tyrosyl-tRNA synthetase (TyrRS) with the Y37V and Q195C mutations (37V195C), which specifically recognize 3-iodo-L-tyrosine rather than L-tyrosine. In the present study, we determined the 3-iodo-L-tyrosine- and L-tyrosine-...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0407039102

    authors: Kobayashi T,Sakamoto K,Takimura T,Sekine R,Kelly VP,Kamata K,Nishimura S,Yokoyama S

    更新日期:2005-02-01 00:00:00

  • Mg2+-sensing mechanism of Mg2+ transporter MgtE probed by molecular dynamics study.

    abstract::Proper regulation of the intracellular ion concentration is essential to maintain life and is achieved by ion transporters that transport their substrates across the membrane in a strictly regulated manner. MgtE is a Mg(2+) transporter that may function in the homeostasis of the intracellular Mg(2+) concentration. A r...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0802991105

    authors: Ishitani R,Sugita Y,Dohmae N,Furuya N,Hattori M,Nureki O

    更新日期:2008-10-07 00:00:00

  • Preventing ventricular fibrillation by flattening cardiac restitution.

    abstract::Ventricular fibrillation is the leading cause of sudden cardiac death. In fibrillation, fragmented electrical waves meander erratically through the heart muscle, creating disordered and ineffective contraction. Theoretical and computer studies, as well as recent experimental evidence, have suggested that fibrillation ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.090492697

    authors: Garfinkel A,Kim YH,Voroshilovsky O,Qu Z,Kil JR,Lee MH,Karagueuzian HS,Weiss JN,Chen PS

    更新日期:2000-05-23 00:00:00

  • Nuclease protection analysis of ribonucleoprotein complexes: use of the cytotoxic ribonuclease alpha-sarcin to determine the binding sites for Escherichia coli ribosomal proteins L5, L18, and L25 on 5S rRNA.

    abstract::A rapid and convenient method has been devised to determine the binding sites for proteins on RNA. The procedure is an adaptation of one used to map DNA-protein complexes by protection against nuclease digestion. The method uses the cytotoxic ribonuclease alpha-sarcin, which hydrolyzes purines in both single- and doub...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.81.2.322

    authors: Huber PW,Wool IG

    更新日期:1984-01-01 00:00:00

  • Aquaporin-4 square array assembly: opposing actions of M1 and M23 isoforms.

    abstract::Osmotic homeostasis in the brain involves movement of water through aquaporin-4 (AQP4) membrane channels. Perivascular astrocyte end-feet contain distinctive orthogonal lattices (square arrays) assembled from 4- to 6-nm intramembrane particles (IMPs) corresponding to individual AQP4 tetramers. Two isoforms of AQP4 res...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2235843100

    authors: Furman CS,Gorelick-Feldman DA,Davidson KG,Yasumura T,Neely JD,Agre P,Rash JE

    更新日期:2003-11-11 00:00:00

  • Covalent linkage of ribonuclease S-peptide to microinjected proteins causes their intracellular degradation to be enhanced during serum withdrawal.

    abstract::The amino-terminal 20 amino acids are required for microinjected ribonuclease A (RNase A) to be taken up by lysosomes and degraded at an enhanced rate during serum withdrawal. We used water-soluble carbodiimides to covalently attach the RNase S-peptide (residues 1-20) to [3H]RNase S-protein (residues 21-124) at unspec...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.83.16.5830

    authors: Backer JM,Dice JF

    更新日期:1986-08-01 00:00:00

  • A key developmental regulator controls the synthesis of the antibiotic erythromycin in Saccharopolyspora erythraea.

    abstract::Saccharopolyspora erythraea makes erythromycin, an antibiotic commonly used in human medicine. Unusually, the erythromycin biosynthetic (ery) cluster lacks a pathway-specific regulatory gene. We isolated a transcriptional regulator of the ery biosynthetic genes from S. erythraea and found that this protein appears to ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0803622105

    authors: Chng C,Lum AM,Vroom JA,Kao CM

    更新日期:2008-08-12 00:00:00

  • International population movements and regional Plasmodium falciparum malaria elimination strategies.

    abstract::Calls for the eradication of malaria require the development of global and regional strategies based on a strong and consistent evidence base. Evidence from the previous global malaria eradication program and more recent transborder control campaigns have shown the importance of accounting for human movement in introd...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1002971107

    authors: Tatem AJ,Smith DL

    更新日期:2010-07-06 00:00:00

  • Political ideology shapes the amplification of the accomplishments of disadvantaged vs. advantaged group members.

    abstract::Recent years have witnessed an increased public outcry in certain quarters about a perceived lack of attention given to successful members of disadvantaged groups relative to equally meritorious members of advantaged groups, exemplified by social media campaigns centered around hashtags, such as #OscarsSoWhite and #Wo...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1818545116

    authors: Kteily NS,Rocklage MD,McClanahan K,Ho AK

    更新日期:2019-01-29 00:00:00

  • Transgenic sorghum plants via microprojectile bombardment.

    abstract::Transgenic sorghum plants have been obtained after microprojectile bombardment of immature zygotic embryos of a drought-resistant sorghum cultivar, P898012. DNA delivery parameters were optimized based on transient expression of R and C1 maize anthocyanin regulatory elements in scutellar cells. The protocol for obtain...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.23.11212

    authors: Casas AM,Kononowicz AK,Zehr UB,Tomes DT,Axtell JD,Butler LG,Bressan RA,Hasegawa PM

    更新日期:1993-12-01 00:00:00

  • Chaperone-enhanced purification of unconventional myosin 15, a molecular motor specialized for stereocilia protein trafficking.

    abstract::Unconventional myosin 15 is a molecular motor expressed in inner ear hair cells that transports protein cargos within developing mechanosensory stereocilia. Mutations of myosin 15 cause profound hearing loss in humans and mice; however, the properties of this motor and its regulation within the stereocilia organelle a...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1409459111

    authors: Bird JE,Takagi Y,Billington N,Strub MP,Sellers JR,Friedman TB

    更新日期:2014-08-26 00:00:00

  • Maize AMEIOTIC1 is essential for multiple early meiotic processes and likely required for the initiation of meiosis.

    abstract::Molecular mechanisms that initiate meiosis have been studied in fungi and mammals, but little is known about the mechanisms directing the meiosis transition in other organisms. To elucidate meiosis initiation in plants, we characterized and cloned the ameiotic1 (am1) gene, which affects the transition to meiosis and p...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0810115106

    authors: Pawlowski WP,Wang CJ,Golubovskaya IN,Szymaniak JM,Shi L,Hamant O,Zhu T,Harper L,Sheridan WF,Cande WZ

    更新日期:2009-03-03 00:00:00

  • Murine leukemia virus morphogenesis: cleavage of P70 in vitro can be accompanied by a shift from a concentrically coiled internal strand ("immature") to a collapsed ("mature") form of the virus core.

    abstract::Disruption of Rauscher leukemia virus (RLV) with low levels of Nonidet P-40 yielded "immature" cores. These cores have a diameter of about 920 A, as opposed to the 1300-A diameter of RLV, possess knob-like protuberances, and contain a concentrically coiled internal strand apposed to the core shell. The two major polyp...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.74.8.3446

    authors: Yoshinaka Y,Luftig RB

    更新日期:1977-08-01 00:00:00

  • Tangential migration of neuronal precursors of glutamatergic neurons in the adult mammalian brain.

    abstract::In a classic model of mammalian brain formation, precursors of principal glutamatergic neurons migrate radially along radial glia fibers whereas GABAergic interneuron precursors migrate tangentially. These migration modes have significant implications for brain function. Here we used clonal lineage tracing of active r...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1508545112

    authors: Sun GJ,Zhou Y,Stadel RP,Moss J,Yong JH,Ito S,Kawasaki NK,Phan AT,Oh JH,Modak N,Reed RR,Toni N,Song H,Ming GL

    更新日期:2015-07-28 00:00:00

  • Recombination involving transposable elements: role of target molecule replication in Tn1 delta Ap-mediated replicon fusion.

    abstract::Donor DNA molecules carrying Tn1 or Tn3 deletion mutants do not need to replicate in order to participate in replicon fusion recombination events during which the Tn1/Tn3 element is duplicated. We have assayed Tn1 delta Ap-mediated replicon fusion events involving plasmid R388 and the bacteriophage lambda-derived plas...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.80.8.2314

    authors: Muster CJ,Shapiro JA,MacHattie LA

    更新日期:1983-04-01 00:00:00

  • Spectrum and chemical composition of the remarkable planetary nebula NGC 6537.

    abstract::Observations with the image tube scanner at the Shane 3-m telescope are combined with data obtained with the International Ultraviolet Explorer to assess the spectrum of the remarkable high-excitation planetary nebula NGC 6537. We have analyzed the spectrum of this nitrogen-rich object with the aid of the theoretical ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.82.8.2202

    authors: Feibelman W,Aller LH,Keyes CD,Czyzak SJ

    更新日期:1985-04-01 00:00:00

  • Antibodies mediate intracellular immunity through tripartite motif-containing 21 (TRIM21).

    abstract::Antibodies provide effective antiviral immunity despite the fact that viruses escape into cells when they infect. Here we show that antibodies remain attached to viruses after cell infection and mediate an intracellular immune response that disables virions in the cytosol. We have discovered that cells possess a cytos...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1014074107

    authors: Mallery DL,McEwan WA,Bidgood SR,Towers GJ,Johnson CM,James LC

    更新日期:2010-11-16 00:00:00

  • The catalytic core of DEMETER guides active DNA demethylation in Arabidopsis.

    abstract::The Arabidopsis DEMETER (DME) DNA glycosylase demethylates the maternal genome in the central cell prior to fertilization and is essential for seed viability. DME preferentially targets small transposons that flank coding genes, influencing their expression and initiating plant gene imprinting. DME also targets interg...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1907290116

    authors: Zhang C,Hung YH,Rim HJ,Zhang D,Frost JM,Shin H,Jang H,Liu F,Xiao W,Iyer LM,Aravind L,Zhang XQ,Fischer RL,Huh JH,Hsieh TF

    更新日期:2019-08-27 00:00:00

  • Cellular mechanisms for resolving phase ambiguity in the owl's inferior colliculus.

    abstract::Both mammals and birds use the interaural time difference (ITD) for localization of sound in the horizontal plane. They may localize either real or phantom sound sources, when the signal consists of a narrow frequency band. This ambiguity does not occur with broadband signals. A plot of impulse rates or amplitude of e...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.97.22.11787

    authors: Peña JL,Konishi M

    更新日期:2000-10-24 00:00:00

  • Targeted deletion in astrocyte intermediate filament (Gfap) alters neuronal physiology.

    abstract::Glial fibrillary acidic protein (GFAP) is a member of the family of intermediate filament structural proteins and is found predominantly in astrocytes of the central nervous system (CNS). To assess the function of GFAP, we created GFAP-null mice using gene targeting in embryonic stem cells. The GFAP-null mice have nor...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.13.6361

    authors: McCall MA,Gregg RG,Behringer RR,Brenner M,Delaney CL,Galbreath EJ,Zhang CL,Pearce RA,Chiu SY,Messing A

    更新日期:1996-06-25 00:00:00

  • Pharmacokinetics, biodistribution, and stability of oligodeoxynucleotide phosphorothioates in mice.

    abstract::We describe preliminary studies of the pharmacokinetics, biodistribution, and excretion of an oligodeoxy-nucleotide phosphorothioate ([S]oligonucleotide) in mice. After either intravenous or intraperitoneal administration of a single dose (30 mg/kg of body weight), [S]oligonucleotide (35S-labeled at each internucleoti...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.88.17.7595

    authors: Agrawal S,Temsamani J,Tang JY

    更新日期:1991-09-01 00:00:00

  • Expression and functional significance of VE-cadherin in aggressive human melanoma cells: role in vasculogenic mimicry.

    abstract::We recently have introduced the term vasculogenic mimicry to describe the unique ability of aggressive melanoma tumor cells to form tubular structures and patterned networks in three-dimensional culture, which "mimics" embryonic vasculogenic networks formed by differentiating endothelial cells. In the current study, w...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.131209798

    authors: Hendrix MJ,Seftor EA,Meltzer PS,Gardner LM,Hess AR,Kirschmann DA,Schatteman GC,Seftor RE

    更新日期:2001-07-03 00:00:00

  • Pattern of gp120 sequence divergence linked to a lack of clinical progression in human immunodeficiency virus type 1 infection.

    abstract::Differential rates of AIDS development and/or T4 lymphocyte depletion in HIV-1-infected individuals remain unexplained. The hypothesis that qualitative differences in selection pressure in vivo may account for different rates of disease progression was addressed in nine eligible study participants from a cohort of 315...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.13.6693

    authors: Wang WK,Essex M,McLane MF,Mayer KH,Hsieh CC,Brumblay HG,Seage G,Lee TH

    更新日期:1996-06-25 00:00:00

  • Localization of cardiac L-type Ca(2+) channels to a caveolar macromolecular signaling complex is required for beta(2)-adrenergic regulation.

    abstract::L-type Ca(2+) channels play a critical role in regulating Ca(2+)-dependent signaling in cardiac myocytes, including excitation-contraction coupling; however, the subcellular localization of cardiac L-type Ca(2+) channels and their regulation are incompletely understood. Caveolae are specialized microdomains of the pla...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0503465103

    authors: Balijepalli RC,Foell JD,Hall DD,Hell JW,Kamp TJ

    更新日期:2006-05-09 00:00:00

  • The sigma factor sigma s affects antibiotic production and biological control activity of Pseudomonas fluorescens Pf-5.

    abstract::Pseudomonas fluorescens Pf-5, a rhizosphere-inhabiting bacterium that suppresses several soilborne pathogens of plants, produces the antibiotics pyrrolnitrin, pyoluteorin, and 2,4-diacetylphloroglucinol. A gene necessary for pyrrolnitrin production by Pf-5 was identified as rpoS, which encodes the stationary-phase sig...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.92.26.12255

    authors: Sarniguet A,Kraus J,Henkels MD,Muehlchen AM,Loper JE

    更新日期:1995-12-19 00:00:00

  • Imaging the Holon string of the Hubbard model.

    abstract::It has been a long-sought goal of quantum simulation to find answers to outstanding questions in condensed-matter physics. A famous example is finding the ground state and the excitations of the two-dimensional (2D) Hubbard model with strong repulsion below half-filling. This system is a doped antiferromagnet and is o...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2004268117

    authors: Ho TL

    更新日期:2020-10-20 00:00:00

  • Modern diversification of the amino acid repertoire driven by oxygen.

    abstract::All extant life employs the same 20 amino acids for protein biosynthesis. Studies on the number of amino acids necessary to produce a foldable and catalytically active polypeptide have shown that a basis set of 7-13 amino acids is sufficient to build major structural elements of modern proteins. Hence, the reasons for...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1717100115

    authors: Granold M,Hajieva P,Toşa MI,Irimie FD,Moosmann B

    更新日期:2018-01-02 00:00:00

  • Structure of the Ty3/Gypsy retrotransposon capsid and the evolution of retroviruses.

    abstract::Retroviruses evolved from long terminal repeat (LTR) retrotransposons by acquisition of envelope functions, and subsequently reinvaded host genomes. Together, endogenous retroviruses and LTR retrotransposons represent major components of animal, plant, and fungal genomes. Sequences from these elements have been exapte...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1900931116

    authors: Dodonova SO,Prinz S,Bilanchone V,Sandmeyer S,Briggs JAG

    更新日期:2019-05-14 00:00:00