Binding and biomimetic cleavage of the RNA poly(U) by synthetic polyimidazoles.

abstract：:The six members of the contactin (CNTN) family of neural cell adhesion molecules are involved in the formation and maintenance of the central nervous system (CNS) and have been linked to mental retardation and neuropsychiatric disorders such as autism. Five of the six CNTNs bind to the homologous receptor protein tyro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lamprianou S,Chatzopoulou E,Thomas JL,Bouyain S,Harroch S

更新日期：2011-10-18 00:00:00

abstract：:Animal mitochondrial DNA (mtDNA) maintains a displacement loop (D loop) at the heavy strand origin of replication. These D loops represent sharply limited synthesis of heavy strands and provide a unique opportunity to examine the termination of DNA synthesis. Direct sizing at the nucleotide level indicates that the 3'...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doda JN,Wright CT,Clayton DA

更新日期：1981-10-01 00:00:00

abstract：:Depression is a debilitating mental illness and is often comorbid with metabolic disorders such as type 2 diabetes. Adiponectin is an adipocyte-derived hormone with antidiabetic and insulin-sensitizing properties. Here we show that adiponectin levels in plasma are reduced in a chronic social-defeat stress model of dep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu J,Guo M,Zhang D,Cheng SY,Liu M,Ding J,Scherer PE,Liu F,Lu XY

更新日期：2012-07-24 00:00:00

abstract：:Micrometeorites with diameter approximately 100-200 microm dominate the flux of extraterrestrial matter on Earth. The vast majority of micrometeorites are chemically, mineralogically, and isotopically related to carbonaceous chondrites, which amount to only 2.5% of meteorite falls. Here, we report the discovery of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gounelle M,Chaussidon M,Morbidelli A,Barrat JA,Engrand C,Zolensky ME,McKeegan KD

更新日期：2009-04-28 00:00:00

abstract：:The rate of leucine incorporation into a specific estrogen-induced protein of immature rat isolated by gel electrophoresis declines rapidly between 2-4 hr after estrogen stimulation in vivo followed by incubation in vitro. Actinomycin D present during the in vitro phase prevents this decline, and elicits a "superinduc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DeAngelo AB,Fujimoto GI

更新日期：1973-01-01 00:00:00

abstract：:The exquisite selectivity and catalytic activity of enzymes have been shaped by the effects of positive and negative selection pressure during the course of evolution. In contrast, enzyme variants engineered by using in vitro screening techniques to accept novel substrates typically display a higher degree of catalyti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Varadarajan N,Gam J,Olsen MJ,Georgiou G,Iverson BL

更新日期：2005-05-10 00:00:00

abstract：:Molecular dynamics simulations show that the desolvation rates of isotopes of Li(+), K(+), Rb(+), Ca(2+), Sr(2+), and Ba(2+) may have a relatively strong dependence on the metal cation mass. This inference is based on the observation that the exchange rate constant, k(wex), for water molecules in the first hydration s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hofmann AE,Bourg IC,DePaolo DJ

更新日期：2012-11-13 00:00:00

abstract：:Understanding and manipulation of the forces assembling DNA/RNA helices have broad implications for biology, medicine, and physics. One subject of significance is the attractive force between dsDNA mediated by polycations of valence ≥ 3. Despite extensive studies, the physical origin of the "like-charge attraction" re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qiu X,Parsegian VA,Rau DC

更新日期：2010-12-14 00:00:00

abstract：:The product of the recA gene of E. coli has been identified by labeling proteins synthesized in UV-treated cells after infection with specialized transducing phages carrying the recA gene. Following infection of UV-treated cells by lambda precA, which carries the recA+ gene, a major protein with a molecular weight of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McEntee K,Hesse JE,Epstein W

更新日期：1976-11-01 00:00:00

abstract：:After prolonged visual adaptation of Drosophila, dramatic long-term changes of in vitro phosphorylation of a 50-kDa brain protein that is identical to the Ca2+/calmodulin-dependent protein kinase (EC 2.7.1.37) can be measured in isolated heads. By selective receptor cell desensitization in blue light, subcellular dist...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Willmund R,Mitschulat H,Schneider K

更新日期：1986-12-01 00:00:00

abstract：:Focal adhesion kinase (FAK) is a nonreceptor tyrosine kinase involved in development and human disease, including cancer. It is currently thought that the four-point one, ezrin, radixin, moesin (FERM)-kinase domain linker, which contains autophosphorylation site tyrosine (Y) 397, is not required for in vivo FAK functi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heim JB,Squirewell EJ,Neu A,Zocher G,Sominidi-Damodaran S,Wyles SP,Nikolova E,Behrendt N,Saunte DM,Lock-Andersen J,Gaonkar KS,Yan H,Sarkaria JN,Krendel M,van Deursen J,Sprangers R,Stehle T,Böttcher RT,Lee JH,Ordog T

更新日期：2017-04-11 00:00:00

abstract：:VEGF and angiopoietin-1 (Ang1) are two major angiogenic factors being investigated for the treatment of myocardial infarction (MI). Targeting VEGF and Ang1 expression in the ischemic myocardium can increase their local therapeutic effects and reduce possible adverse effects. Adeno-associated viral vectors (AAVs) expre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tao Z,Chen B,Tan X,Zhao Y,Wang L,Zhu T,Cao K,Yang Z,Kan YW,Su H

更新日期：2011-02-01 00:00:00

abstract：:Zfp57 is a maternal-zygotic effect gene that maintains genomic imprinting. Here we report that Zfp57 mutants exhibited a variety of cardiac defects including atrial septal defect (ASD), ventricular septal defect (VSD), thin myocardium, and reduced trabeculation. Zfp57 maternal-zygotic mutant embryos displayed more sev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shamis Y,Cullen DE,Liu L,Yang G,Ng SF,Xiao L,Bell FT,Ray C,Takikawa S,Moskowitz IP,Cai CL,Yang X,Li X

更新日期：2015-04-21 00:00:00

abstract：:The number of sensory receptor genes varies extensively among different mammalian species. This variation is believed to be caused partly by physiological requirements of animals and partly by genomic drift due to random duplication and deletion of genes. If the contribution of genomic drift is substantial, each speci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nozawa M,Kawahara Y,Nei M

更新日期：2007-12-18 00:00:00

abstract：:Prostaglandin E1 and the beta-adrenergic hormone l-isoproterenol stimulated cyclic AMP formation in both nucleated and enucleated myeloid leukemic cells that could be induced to differentiate normally to mature cells by the macrophage- and granulocyte-inducing protein MGI (MGI+D+ cells). Enucleated as well as nucleate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simantov R,Shkolnik T,Sachs L

更新日期：1980-08-01 00:00:00

abstract：:The retinoblastoma gene product (the RB protein) is phosphorylated in a cell cycle-dependent manner and this modification is believed to be important for cells to progress through the cell cycle. We found that purified cdk2 (cyclin-dependent kinase/cell division kinase 2) can phosphorylate the RB protein in vitro at t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Akiyama T,Ohuchi T,Sumida S,Matsumoto K,Toyoshima K

更新日期：1992-09-01 00:00:00

abstract：:The topology of the tight junction is probed by introducing dipicrylamine (dpa-), a lipid-soluble anion, into the membranes of hepatocyte pairs in culture. Once partitioned into the membrane, dpa- ions are free to move in the hydrophobic core of the membrane, where their mobile charges greatly increase membrane capaci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Turin L,Béhé P,Plonsky I,Dunina-Barkovskaya A

更新日期：1991-10-15 00:00:00

abstract：:Infants' sensitivity to contingent reactivity as an indicator of intentional agency has been demonstrated by numerous referential gaze-following studies. Here we propose that variability of signal sequences in a turn-taking exchange provides an informative cue for infants to recognize interactions that may involve com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tauzin T,Gergely G

更新日期：2019-07-30 00:00:00

abstract：:The response of the nematic twist-bend ([Formula: see text]) phase to an applied field can provide important insight into the structure of this liquid and may bring us closer to understanding mechanisms generating mirror symmetry breaking in a fluid of achiral molecules. Here we investigate theoretically how an extern...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pająk G,Longa L,Chrzanowska A

更新日期：2018-10-30 00:00:00

abstract：:Estimation of evolutionary distances has always been a major issue in the study of molecular evolution because evolutionary distances are required for estimating the rate of evolution in a gene, the divergence dates between genes or organisms, and the relationships among genes or organisms. Other closely related issue...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gu X,Li WH

更新日期：1998-05-26 00:00:00

abstract：:We introduce a quantitative framework for assessing the generation of crossovers in DNA shuffling experiments. The approach uses free energy calculations and complete sequence information to model the annealing process. Statistics obtained for the annealing events then are combined with a reassembly algorithm to infer...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moore GL,Maranas CD,Lutz S,Benkovic SJ

更新日期：2001-03-13 00:00:00

abstract：:Arabinose-binding protein, maltose-binding protein, and lambda receptor are synthesized in vitro on membrane-bound polysomes from Escherichia coli. All three proteins are exported from the cytoplasm of E. coli and all three are made in vitro in a form a few thousand daltons larger than the authentic protein. The large...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Randall LL,Hardy SJ,Josefsson LG

更新日期：1978-03-01 00:00:00

abstract：:Saccharomyces cerevisiae possesses a high-affinity estrogen binding protein and an endogenous ligand that displaces [3H]estradiol from both the yeast binding protein and mammalian estrogen receptors. Semipurified preparations of this ligand have been shown to exhibit estrogenic activity in mammalian systems. We now de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feldman D,Tökés LG,Stathis PA,Miller SC,Kurz W,Harvey D

更新日期：1984-08-01 00:00:00

abstract：:Converging TGF-beta and insulin-like neuroendocrine signaling pathways regulate whether Caenorhabditis elegans develops reproductively or arrests at the dauer larval stage. We examined whether neurotransmitters act in the dauer entry or recovery pathways. Muscarinic agonists promote recovery from dauer arrest induced ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tissenbaum HA,Hawdon J,Perregaux M,Hotez P,Guarente L,Ruvkun G

更新日期：2000-01-04 00:00:00

abstract：:Overproduction or deficiency of many chaperones and other cellular components cure the yeast prions [PSI+] (formed by Sup35p) or [URE3] (based on Ure2p). However, at normal expression levels, Btn2p and Cur1p eliminate most newly arising [URE3] variants but do not cure [PSI+], even after overexpression. Deficiency or o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gorkovskiy A,Reidy M,Masison DC,Wickner RB

更新日期：2017-05-23 00:00:00

abstract：:Two Chinese hamster cell lines, G1+-1 and CHO, have been grown in the presence of low concentrations of hydroxyurea to determine how a slowing DNA synthesis (i.e., a lengthening of the S period) affects the length of the G1 period. Hydroxyurea concentrations of approximately 10 microM do not alter the generation times...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stancel GM,Prescott DM,Liskay RM

更新日期：1981-10-01 00:00:00

abstract：:A synthetic polymer has been prepared which contains dodecyl groups (to bind small substrate molecules) and methyleneimidazole side chains (as nucleophilic catalytic sites) linked to a polyethyleneimine framework. This macromolecule, with a high local concentration of binding and catalytic groups, catalyzes the hydrol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klotz IM,Royer GP,Scarpa IS

更新日期：1971-02-01 00:00:00

abstract：:Hepatocyte growth factor (HGF) is an activating ligand of the Met receptor tyrosine kinase, whose activity is essential for normal tissue development and organ regeneration but abnormal activation of Met has been implicated in growth, invasion, and metastasis of many types of solid tumors. HGF has two natural splice v...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tolbert WD,Daugherty-Holtrop J,Gherardi E,Vande Woude G,Xu HE

更新日期：2010-07-27 00:00:00

abstract：:The core protein of a small chondroitin/dermatan sulfate proteoglycan expressed by human fibroblasts and present in extracellular matrices in association with collagen has been cloned from a lambda gt11 fibroblast cDNA library. cDNA clones were isolated by use of antibodies specific for the intact proteoglycan and ant...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krusius T,Ruoslahti E

更新日期：1986-10-01 00:00:00

abstract：:Glucocorticoids are commonly used antiinflammatory agents whose use is limited by side effects. We have developed a series of glucocorticoid receptor (GR) ligands that retain the strong antiinflammatory activity of conventional glucocorticoids with reduced side effects. We present a compound, LGD5552, that binds the r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miner JN,Ardecky B,Benbatoul K,Griffiths K,Larson CJ,Mais DE,Marschke K,Rosen J,Vajda E,Zhi L,Negro-Vilar A

更新日期：2007-12-04 00:00:00