当前位置： SCI文献检索 > NATURE期刊下所有文献 > Dependence of Z-DNA antibody binding to polytene chromosomes on acid fixation and DNA torsional strain.

Dependence of Z-DNA antibody binding to polytene chromosomes on acid fixation and DNA torsional strain.

Abstract：

:There is considerable interest in the existence and significance of alternative conformations of DNA to the right-handed B-form described originally by Watson and Crick. The indirect immunofluorescence observations of Nordheim et al., Arndt-Jovin et al. and Lemeunier et al. that antibodies against left-handed Z-DNA bind to polytene chromosomes have thus assumed considerable importance. However, there is a paradox: some workers observe Z-DNA in interbands and others in bands. We report here that binding of Z-DNA antibodies to Drosophila polytene chromosomes prepared without acid fixation is at background level, and that following acid fixation the same antibody treatment leads to intense fluorescence. Depending on the extent of exposure to 45% acetic acid, fluorescence can occur primarily in interbands or in bands. Furthermore, antibody binding is dependent on elastic torsional strain in the DNA molecules.

journal_name

Nature

journal_title

Nature

authors

Hill RJ,Stollar BD

doi

10.1038/305338a0

subject

Has Abstract

pub_date

1983-09-22 00:00:00

pages

338-40

issue

5932

eissn

0028-0836

issn

1476-4687

journal_volume

305

pub_type

杂志文章

相关文献

NATURE文献大全
  • A reversibly antigen-responsive hydrogel.

    abstract：:Stimuli-responsive hydrogels that undergo abrupt changes in volume in response to external stimuli such as pH, temperature and solvent composition have potential applications in biomedicine and the creation of 'intelligent' materials systems, for example as media for drug delivery, separation processes and protein imm...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/21619

    authors： Miyata T,Asami N,Uragami T

    更新日期：1999-06-24 00:00:00

  • Brain control of humoral immune responses amenable to behavioural modulation.

    abstract：:It has been speculated that brain activities might directly control adaptive immune responses in lymphoid organs, although there is little evidence for this. Here we show that splenic denervation in mice specifically compromises the formation of plasma cells during a T cell-dependent but not T cell-independent immune ...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/s41586-020-2235-7

    authors： Zhang X,Lei B,Yuan Y,Zhang L,Hu L,Jin S,Kang B,Liao X,Sun W,Xu F,Zhong Y,Hu J,Qi H

    更新日期：2020-05-01 00:00:00

  • K-Ras(G12C) inhibitors allosterically control GTP affinity and effector interactions.

    abstract：:Somatic mutations in the small GTPase K-Ras are the most common activating lesions found in human cancer, and are generally associated with poor response to standard therapies. Efforts to target this oncogene directly have faced difficulties owing to its picomolar affinity for GTP/GDP and the absence of known alloster...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature12796

    authors： Ostrem JM,Peters U,Sos ML,Wells JA,Shokat KM

    更新日期：2013-11-28 00:00:00

  • Hinf family: a novel repeated DNA family of the human genome.

    abstract：:The isolation of a mutant adenovirus carrying an insertion of cellular DNA has led to the identification of a new family of human repetitive sequences, which are found tandemly arranged in the genome. The sequence of the viral insert resembles that of eukaryotic transposable elements. ...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/302587a0

    authors： Shimizu Y,Yoshida K,Ren CS,Fujinaga K,Rajagopalan S,Chinnadurai G

    更新日期：1983-04-14 00:00:00

  • Site-specific group selection drives locally adapted group compositions.

    abstract：:Group selection may be defined as selection caused by the differential extinction or proliferation of groups. The socially polymorphic spider Anelosimus studiosus exhibits a behavioural polymorphism in which females exhibit either a 'docile' or 'aggressive' behavioural phenotype. Natural colonies are composed of a mix...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature13811

    authors： Pruitt JN,Goodnight CJ

    更新日期：2014-10-16 00:00:00

  • Room-temperature electronic phase transitions in the continuous phase diagrams of perovskite manganites

    abstract：:Highly correlated electronic systems--such as transition-metal oxides that are doped Mott insulators--are complex systems which exhibit puzzling phenomena, including high-temperature superconductivity and colossal magnetoresistivity. Recent studies suggest that in such systems collective electronic phenomena are impor...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/35021018

    authors： Yoo YK,Duewer F,Yang H,Yi D,Li JW,Xiang XD

    更新日期：2000-08-17 00:00:00

  • TFIIIC relieves repression of U6 snRNA transcription by chromatin.

    abstract：:The U6 small nuclear (sn)RNA gene (SNR6) from the yeast Saccharomyces cerevisiae is transcribed by RNA polymerase III in vivo. This gene is unusual in having a TATA box at position -30, and an essential B-block element located downstream of the T-rich termination signal. The B block is one of the two intragenic promot...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/362475a0

    authors： Burnol AF,Margottin F,Huet J,Almouzni G,Prioleau MN,Méchali M,Sentenac A

    更新日期：1993-04-01 00:00:00

  • Evolution of the MHC class I genes of a New World primate from ancestral homologues of human non-classical genes.

    abstract：:The products of the classical human major histocompatibility complex (MHC) class I genes (HLA-A, -B, -C) are highly polymorphic molecules that bind peptides and present them to T lymphocytes. The non-polymorphic, non-classical MHC class I gene products (HLA-E, -F, -G) are not restricting elements for the majority of T...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/346060a0

    authors： Watkins DI,Chen ZW,Hughes AL,Evans MG,Tedder TF,Letvin NL

    更新日期：1990-07-05 00:00:00

  • Monoclonal antibody and ligand binding sites of the T cell erythrocyte receptor (CD2).

    abstract：:The human T cell erythrocyte receptor (CD2 antigen) allows thymocytes and mature T cells to adhere to thymic epithelium and target cells through a cell surface protein, LFA-3 (refs 1-6). Monoclonal antibodies recognizing CD2 can either block adhesion or, in certain combinations, induce an antigen-independent T cell ac...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/329842a0

    authors： Peterson A,Seed B

    更新日期：1987-10-29 00:00:00

  • Crystal structure of a yeast TFIIA/TBP/DNA complex.

    abstract：:The X-ray crystal structure of the transcription factor IIA (TFIIA) in complex with the TATA-box-binding protein (TBP) and TATA-element DNA is presented at 2.5 A resolution. TFIIA is composed of a beta-barrel and a four-helix bundle motif that together have a boot-like appearance. The beta-barrel extends the TBP beta-...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/381127a0

    authors： Tan S,Hunziker Y,Sargent DF,Richmond TJ

    更新日期：1996-05-09 00:00:00

  • Wolbachia variability and host effects on crossing type in Culex mosquitoes.

    abstract：:Wolbachia is a common maternally inherited bacterial symbiont able to induce crossing sterilities known as cytoplasmic incompatibility (CI) in insects. Wolbachia-modified sperm are unable to complete fertilization of uninfected ova, but a rescue function allows infected eggs to develop normally. By providing a reprodu...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature03629

    authors： Sinkins SP,Walker T,Lynd AR,Steven AR,Makepeace BL,Godfray HC,Parkhill J

    更新日期：2005-07-14 00:00:00

  • Neonatal sunburn and melanoma in mice.

    abstract：:Retrospective epidemiological data have indicated that cutaneous malignant melanoma may arise as a consequence of intense, intermittent exposure of the skin to ultraviolet radiation, particularly in children, rather than from the cumulative lifetime exposure that is associated with other forms of skin cancer. Here we ...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/35095108

    authors： Noonan FP,Recio JA,Takayama H,Duray P,Anver MR,Rush WL,De Fabo EC,Merlino G

    更新日期：2001-09-20 00:00:00

  • Holocene dwarf mammoths from Wrangel Island in the Siberian Arctic.

    abstract：:THE cause of extinction of the woolly mammoth, Mammuthus primigenius (Blumenbach), is still debated. A major environmental change at the Pleistocene-Holocene boundary, hunting by early man, or both together are among the main explanations that have been suggested. But hardly anyone has doubted that mammoths had become...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/362337a0

    authors： Vartanyan SL,Garutt VE,Sher AV

    更新日期：1993-03-25 00:00:00

  • Wave-particle duality of C(60) molecules.

    abstract：:Quantum superposition lies at the heart of quantum mechanics and gives rise to many of its paradoxes. Superposition of de Broglie matter waves' has been observed for massive particles such as electrons, atoms and dimers, small van der Waals clusters, and neutrons. But matter wave interferometry with larger objects has...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/44348

    authors： Arndt M,Nairz O,Vos-Andreae J,Keller C,van der Zouw G,Zeilinger A

    更新日期：1999-10-14 00:00:00

  • Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases.

    abstract：:Holliday junctions (HJs) are key intermediates in homologous recombination and are especially important for the production of crossover recombinants. Bacterial RecA family proteins promote the formation and branch migration of HJs in vitro by catalysing a reciprocal DNA-strand exchange reaction between two duplex DNA ...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature06609

    authors： Murayama Y,Kurokawa Y,Mayanagi K,Iwasaki H

    更新日期：2008-02-21 00:00:00

  • The genomic signature of dog domestication reveals adaptation to a starch-rich diet.

    abstract：:The domestication of dogs was an important episode in the development of human civilization. The precise timing and location of this event is debated and little is known about the genetic changes that accompanied the transformation of ancient wolves into domestic dogs. Here we conduct whole-genome resequencing of dogs...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature11837

    authors： Axelsson E,Ratnakumar A,Arendt ML,Maqbool K,Webster MT,Perloski M,Liberg O,Arnemo JM,Hedhammar A,Lindblad-Toh K

    更新日期：2013-03-21 00:00:00

  • Mastering the game of Go without human knowledge.

    abstract：:A long-standing goal of artificial intelligence is an algorithm that learns, tabula rasa, superhuman proficiency in challenging domains. Recently, AlphaGo became the first program to defeat a world champion in the game of Go. The tree search in AlphaGo evaluated positions and selected moves using deep neural networks....

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature24270

    authors： Silver D,Schrittwieser J,Simonyan K,Antonoglou I,Huang A,Guez A,Hubert T,Baker L,Lai M,Bolton A,Chen Y,Lillicrap T,Hui F,Sifre L,van den Driessche G,Graepel T,Hassabis D

    更新日期：2017-10-18 00:00:00

  • Precise dating of Dansgaard-Oeschger climate oscillations in western Europe from stalagmite data.

    abstract：:The signature of Dansgaard-Oeschger events--millennial-scale abrupt climate oscillations during the last glacial period--is well established in ice cores and marine records. But the effects of such events in continental settings are not as clear, and their absolute chronology is uncertain beyond the limit of (14)C dat...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature01391

    authors： Genty D,Blamart D,Ouahdi R,Gilmour M,Baker A,Jouzel J,Van-Exter S

    更新日期：2003-02-20 00:00:00

  • Mechanism of transfer RNA maturation by CCA-adding enzyme without using an oligonucleotide template.

    abstract：:Transfer RNA nucleotidyltransferases (CCA-adding enzymes) are responsible for the maturation or repair of the functional 3' end of tRNAs by means of the addition of the essential nucleotides CCA. However, it is unclear how tRNA nucleotidyltransferases polymerize CCA onto the 3' terminus of immature tRNAs without using...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature02711

    authors： Xiong Y,Steitz TA

    更新日期：2004-08-05 00:00:00

  • Temporally distinct pre- and post-synaptic mechanisms maintain long-term potentiation.

    abstract：:Long-term potentiation (LTP) in the hippocampus is widely studied as the mechanisms involved in its induction and maintenance are believed to underlie fundamental properties of learning and memory in vertebrates. Most synapses that exhibit LTP use an excitatory amino-acid neurotransmitter that acts on two types of rec...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/338500a0

    authors： Davies SN,Lester RA,Reymann KG,Collingridge GL

    更新日期：1989-04-06 00:00:00

  • The sodium channel and intracellular H+ blockage in squid axons.

    abstract：:Sodium channels in plasma membranes can be blocked by a large variety of toxins and local anaesthetics. This property, however, is not confined to relatively large molecules. For instance, extracellularly applied small ions like hydrogen may also prevent the passive transport of permeant cations across open Na+ channe...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/287062a0

    authors： Wanke E,Carbone E,Testa PL

    更新日期：1980-09-04 00:00:00

  • Plate tectonics, damage and inheritance.

    abstract：:The initiation of plate tectonics on Earth is a critical event in our planet's history. The time lag between the first proto-subduction (about 4 billion years ago) and global tectonics (approximately 3 billion years ago) suggests that plates and plate boundaries became widespread over a period of 1 billion years. The ...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature13072

    authors： Bercovici D,Ricard Y

    更新日期：2014-04-24 00:00:00

  • Enhancing crystal growth using polyelectrolyte solutions and shear flow.

    abstract：:The ability to grow properly sized and good quality crystals is one of the cornerstones of single-crystal diffraction, is advantageous in many industrial-scale chemical processes1-3, and is important for obtaining institutional approvals of new drugs for which high-quality crystallographic data are required4-7. Typica...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/s41586-020-2042-1

    authors： Sun JK,Sobolev YI,Zhang W,Zhuang Q,Grzybowski BA

    更新日期：2020-03-01 00:00:00

  • A chromatin remodelling complex that loads cohesin onto human chromosomes.

    abstract：:Nucleosomal DNA is arranged in a higher-order structure that presents a barrier to most cellular processes involving protein DNA interactions. The cellular machinery involved in sister chromatid cohesion, the cohesin complex, also requires access to the nucleosomal DNA to perform its function in chromosome segregation...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature01024

    authors： Hakimi MA,Bochar DA,Schmiesing JA,Dong Y,Barak OG,Speicher DW,Yokomori K,Shiekhattar R

    更新日期：2002-08-29 00:00:00

  • A global resource allocation strategy governs growth transition kinetics of Escherichia coli.

    abstract：:A grand challenge of systems biology is to predict the kinetic responses of living systems to perturbations starting from the underlying molecular interactions. Changes in the nutrient environment have long been used to study regulation and adaptation phenomena in microorganisms and they remain a topic of active inves...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature24299

    authors： Erickson DW,Schink SJ,Patsalo V,Williamson JR,Gerland U,Hwa T

    更新日期：2017-11-02 00:00:00

  • Molecular mechanism of cAMP modulation of HCN pacemaker channels.

    abstract：:Hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both heart and brain. All four family members contain both a core transmembrane segment domain, homologous to the S1-S6 regions of voltage-gated K+ channels, and a carboxy-terminal 120 amino-acid cyclic nu...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/35081088

    authors： Wainger BJ,DeGennaro M,Santoro B,Siegelbaum SA,Tibbs GR

    更新日期：2001-06-14 00:00:00

  • Two-million-year-old snapshots of atmospheric gases from Antarctic ice.

    abstract：:Over the past eight hundred thousand years, glacial-interglacial cycles oscillated with a period of one hundred thousand years ('100k world'1). Ice core and ocean sediment data have shown that atmospheric carbon dioxide, Antarctic temperature, deep ocean temperature, and global ice volume correlated strongly with each...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/s41586-019-1692-3

    authors： Yan Y,Bender ML,Brook EJ,Clifford HM,Kemeny PC,Kurbatov AV,Mackay S,Mayewski PA,Ng J,Severinghaus JP,Higgins JA

    更新日期：2019-10-01 00:00:00

  • Fetal bovine serum alternatives.

    abstract：:With the current shortage in fetal bovine serum, researchers are turning to a variety of options for cell and tissue culture. ...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/334547a0

    authors： Jayme DW,Epstein DA,Conrad DR

    更新日期：1988-08-11 00:00:00

  • Complete quantum control of a single quantum dot spin using ultrafast optical pulses.

    abstract：:A basic requirement for quantum information processing systems is the ability to completely control the state of a single qubit. For qubits based on electron spin, a universal single-qubit gate is realized by a rotation of the spin by any angle about an arbitrary axis. Driven, coherent Rabi oscillations between two sp...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature07530

    authors： Press D,Ladd TD,Zhang B,Yamamoto Y

    更新日期：2008-11-13 00:00:00

  • Extensive variation of human immunodeficiency virus type-1 in vivo.

    abstract：:Genotypic variation among independent isolates of human immunodeficiency virus type-1 (HIV-1) is well known, but its molecular basis and biological consequences are poorly understood. We examined the genesis of molecular variation in HIV-1 by sequential virus isolations from two chronically infected individuals and an...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/334440a0

    authors： Saag MS,Hahn BH,Gibbons J,Li Y,Parks ES,Parks WP,Shaw GM

    更新日期：1988-08-04 00:00:00