Crystal structure of a yeast TFIIA/TBP/DNA complex.


:The X-ray crystal structure of the transcription factor IIA (TFIIA) in complex with the TATA-box-binding protein (TBP) and TATA-element DNA is presented at 2.5 A resolution. TFIIA is composed of a beta-barrel and a four-helix bundle motif that together have a boot-like appearance. The beta-barrel extends the TBP beta-sheet and bridges over the DNA major groove immediately upstream of the TATA box. The four-helix bundle contributes substantially to the surface of the complex available for interaction with additional transcription factors.






Tan S,Hunziker Y,Sargent DF,Richmond TJ




Has Abstract


1996-05-09 00:00:00














  • Electrostatic interactions in the assembly of haemoglobin.

    abstract::Haemoglobin is the prototype of an allosteric protein in which cooperative behaviour depends on interaction between unlike subunits. Here we present haematological and biochemical evidence that electrostatic interactions are an important determinant of haemoglobin assembly. Individuals heterozygous for positively char...


    pub_type: 杂志文章


    authors: Bunn HF,McDonald MJ

    更新日期:1983-12-01 00:00:00

  • A nucleotide regulatory site for somatostatin inhibition of adenylate cyclase in S49 lymphoma cells.

    abstract::The cyc- variants of S49 lymphoma cells have served as powerful tools for studying the components and mechanisms of hormone-induced adenylate cyclase stimulation, as these cells are deficient in the guanine nucleotide regulatory site (Ns) mediating hormone, guanine nucleotide, cholera toxin and fluoride-induced stimul...


    pub_type: 杂志文章


    authors: Jakobs KH,Aktories K,Schultz G

    更新日期:1983-05-12 00:00:00

  • Non-thermal melting in semiconductors measured at femtosecond resolution.

    abstract::Ultrafast time-resolved optical spectroscopy has revealed new classes of physical, chemical and biological reactions, in which directed, deterministic motions of atoms have a key role. This contrasts with the random, diffusive motion of atoms across activation barriers that typically determines kinetic rates on slower...


    pub_type: 杂志文章


    authors: Rousse A,Rischel C,Fourmaux S,Uschmann I,Sebban S,Grillon G,Balcou P,Förster E,Geindre JP,Audebert P,Gauthier JC,Hulin D

    更新日期:2001-03-01 00:00:00

  • Phylogenetic evidence for Sino-Tibetan origin in northern China in the Late Neolithic.

    abstract::The study of language origin and divergence is important for understanding the history of human populations and their cultures. The Sino-Tibetan language family is the second largest in the world after Indo-European, and there is a long-running debate about its phylogeny and the time depth of its original divergence1....


    pub_type: 历史文章,杂志文章


    authors: Zhang M,Yan S,Pan W,Jin L

    更新日期:2019-05-01 00:00:00

  • Molecular cloning and expression of the major protein kinase C substrate of platelets.

    abstract::In platelets, agonists that stimulate phosphoinositide turnover cause the rapid phosphorylation of a protein of apparent relative molecular mass (Mr) 40-47,000, called P47, by protein kinase C (PKC). Diverse identities have been ascribed to P47 including lipocortin, inositol 1,4,5-trisphosphate 5-phosphomonoesterase, ...


    pub_type: 杂志文章


    authors: Tyers M,Rachubinski RA,Stewart MI,Varrichio AM,Shorr RG,Haslam RJ,Harley CB

    更新日期:1988-06-02 00:00:00

  • US genetic regulations: bacterial field trial to go ahead.

    abstract::The National Institutes of Health's Recombinant DNA Advisory Committee (RAC) has approved a commercial proposal by Advanced Genetic Sciences Inc. to field-test recombinant ice-nucleating bacteria. Its decision came two weeks after a federal judge halted a similar trial by researchers from the University of California...


    pub_type: 新闻


    authors: Budiansky S

    更新日期:1984-06-07 00:00:00

  • One rogue agent suffices for genomic chaos.

    abstract:: ...


    pub_type: 评论,新闻


    authors: Larrimore KE,Rancati G

    更新日期:2019-02-01 00:00:00

  • Clarifying the glass-transition behaviour of water by comparison with hyperquenched inorganic glasses.

    abstract::The formation of glasses is normal for substances that remain liquid over a wide temperature range (the 'good glassformers') and can be induced for most liquids if cooling is fast enough to bypass crystallization. During reheating but still below the melting point, good glassformers exhibit glass transitions as they a...


    pub_type: 杂志文章


    authors: Yue Y,Angell CA

    更新日期:2004-02-19 00:00:00

  • A complex containing N-CoR, mSin3 and histone deacetylase mediates transcriptional repression.

    abstract::Transcriptional repression by nuclear receptors has been correlated to binding of the putative co-repressor, N-CoR. A complex has been identified that contains N-CoR, the Mad presumptive co-repressor mSin3, and the histone deacetylase mRPD3, and which is required for both nuclear receptor- and Mad-dependent repression...


    pub_type: 杂志文章


    authors: Heinzel T,Lavinsky RM,Mullen TM,Söderstrom M,Laherty CD,Torchia J,Yang WM,Brard G,Ngo SD,Davie JR,Seto E,Eisenman RN,Rose DW,Glass CK,Rosenfeld MG

    更新日期:1997-05-01 00:00:00

  • Magnetic trapping of neutrons

    abstract::Accurate measurement of the lifetime of the neutron (which is unstable to beta decay) is important for understanding the weak nuclear force and the creation of matter during the Big Bang. Previous measurements of the neutron lifetime have mainly been limited by certain systematic errors; however, these could in princi...


    pub_type: 杂志文章


    authors: Huffman PR,Brome CR,Butterworth JS,Coakley KJ,Dewey MS,Dzhosyuk SN,Golub R,Greene GL,Habicht K,Lamoreaux SK,Mattoni CE,McKinsey DN,Wietfeldt FE,Doyle JM

    更新日期:2000-01-06 00:00:00

  • The evolutionary history of lethal metastatic prostate cancer.

    abstract::Cancers emerge from an ongoing Darwinian evolutionary process, often leading to multiple competing subclones within a single primary tumour. This evolutionary process culminates in the formation of metastases, which is the cause of 90% of cancer-related deaths. However, despite its clinical importance, little is known...


    pub_type: 杂志文章


    authors: Gundem G,Van Loo P,Kremeyer B,Alexandrov LB,Tubio JMC,Papaemmanuil E,Brewer DS,Kallio HML,Högnäs G,Annala M,Kivinummi K,Goody V,Latimer C,O'Meara S,Dawson KJ,Isaacs W,Emmert-Buck MR,Nykter M,Foster C,Kote-Jarai Z,

    更新日期:2015-04-16 00:00:00

  • Productive dual infection of human CD4+ T lymphocytes by HIV-1 and HHV-6.

    abstract::Although infection by HIV-1 has been implicated as the primary cause of AIDS and related disorders, cofactorial mechanisms may be involved in the pathogenesis of the disease. For example, several viruses commonly detected in AIDS patients and capable of transactivating the long terminal repeat of HIV-1, such as herpes...


    pub_type: 杂志文章


    authors: Lusso P,Ensoli B,Markham PD,Ablashi DV,Salahuddin SZ,Tschachler E,Wong-Staal F,Gallo RC

    更新日期:1989-01-26 00:00:00

  • Solar eclipses of Phobos and Deimos observed from the surface of Mars.

    abstract::The small martian satellites Phobos and Deimos orbit in synchronous rotation with inclinations of only 0.01 degrees and 0.92 degrees , respectively, relative to the planet's equatorial plane. Thus, an observer at near-equatorial latitudes on Mars could occasionally observe solar eclipses by these satellites (see ref. ...


    pub_type: 杂志文章


    authors: Bell JF 3rd,Lemmon MT,Duxbury TC,Hubbard MY,Wolff MJ,Squyres SW,Craig L,Ludwinski JM

    更新日期:2005-07-07 00:00:00

  • An outburst from a massive star 40 days before a supernova explosion.

    abstract::Some observations suggest that very massive stars experience extreme mass-loss episodes shortly before they explode as supernovae, as do several models. Establishing a causal connection between these mass-loss episodes and the final explosion would provide a novel way to study pre-supernova massive-star evolution. Her...


    pub_type: 杂志文章


    authors: Ofek EO,Sullivan M,Cenko SB,Kasliwal MM,Gal-Yam A,Kulkarni SR,Arcavi I,Bildsten L,Bloom JS,Horesh A,Howell DA,Filippenko AV,Laher R,Murray D,Nakar E,Nugent PE,Silverman JM,Shaviv NJ,Surace J,Yaron O

    更新日期:2013-02-07 00:00:00

  • A stomatin-domain protein essential for touch sensation in the mouse.

    abstract::Touch and mechanical pain are first detected at our largest sensory surface, the skin. The cell bodies of sensory neurons that detect such stimuli are located in the dorsal root ganglia, and subtypes of these neurons are specialized to detect specific modalities of mechanical stimuli. Molecules have been identified th...


    pub_type: 杂志文章


    authors: Wetzel C,Hu J,Riethmacher D,Benckendorff A,Harder L,Eilers A,Moshourab R,Kozlenkov A,Labuz D,Caspani O,Erdmann B,Machelska H,Heppenstall PA,Lewin GR

    更新日期:2007-01-11 00:00:00

  • Site-specific group selection drives locally adapted group compositions.

    abstract::Group selection may be defined as selection caused by the differential extinction or proliferation of groups. The socially polymorphic spider Anelosimus studiosus exhibits a behavioural polymorphism in which females exhibit either a 'docile' or 'aggressive' behavioural phenotype. Natural colonies are composed of a mix...


    pub_type: 杂志文章


    authors: Pruitt JN,Goodnight CJ

    更新日期:2014-10-16 00:00:00

  • TFIIIC relieves repression of U6 snRNA transcription by chromatin.

    abstract::The U6 small nuclear (sn)RNA gene (SNR6) from the yeast Saccharomyces cerevisiae is transcribed by RNA polymerase III in vivo. This gene is unusual in having a TATA box at position -30, and an essential B-block element located downstream of the T-rich termination signal. The B block is one of the two intragenic promot...


    pub_type: 杂志文章


    authors: Burnol AF,Margottin F,Huet J,Almouzni G,Prioleau MN,Méchali M,Sentenac A

    更新日期:1993-04-01 00:00:00

  • Do 'lipidic particles' represent intermembrane attachment sites?

    abstract::Although freeze-fracture electron microscopy is normally analysed with the presumption that particles represent proteins embedded within the membrane, particles which appeared to represent inverted lipid micelles within membranes have been reported. I have now confirmed the occurrence of particles in protein-free lipo...


    pub_type: 杂志文章


    authors: Miller RG

    更新日期:1980-09-11 00:00:00

  • Clonal interaction in tumours.

    abstract::The development of cancer is contingent on the emergence of at least one clone of transformed cells. One method used to investigate whether human tumours are monoclonal depends on the mosaicism in the normal tissues of women heterozygous for the two forms of the enzyme glucose-6-phosphate dehydrogenase (G-6-PD). This ...


    pub_type: 杂志文章


    authors: Woodruff MF,Ansell JD,Forbes GM,Gordon JC,Burton DI,Micklem HS

    更新日期:1982-10-28 00:00:00

  • GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas.

    abstract::Growth of basal cell carcinomas (BCCs) requires high levels of hedgehog (HH) signalling through the transcription factor GLI. Although inhibitors of membrane protein smoothened (SMO) effectively suppress HH signalling, early tumour resistance illustrates the need for additional downstream targets for therapy. Here we ...


    pub_type: 杂志文章


    authors: Atwood SX,Li M,Lee A,Tang JY,Oro AE

    更新日期:2013-02-28 00:00:00

  • Fructose-1,6-bisphosphatase opposes renal carcinoma progression.

    abstract::Clear cell renal cell carcinoma (ccRCC), the most common form of kidney cancer, is characterized by elevated glycogen levels and fat deposition. These consistent metabolic alterations are associated with normoxic stabilization of hypoxia-inducible factors (HIFs) secondary to von Hippel-Lindau (VHL) mutations that occu...


    pub_type: 杂志文章


    authors: Li B,Qiu B,Lee DS,Walton ZE,Ochocki JD,Mathew LK,Mancuso A,Gade TP,Keith B,Nissim I,Simon MC

    更新日期:2014-09-11 00:00:00

  • The interaction landscape between transcription factors and the nucleosome.

    abstract::Nucleosomes cover most of the genome and are thought to be displaced by transcription factors in regions that direct gene expression. However, the modes of interaction between transcription factors and nucleosomal DNA remain largely unknown. Here we systematically explore interactions between the nucleosome and 220 tr...


    pub_type: 杂志文章


    authors: Zhu F,Farnung L,Kaasinen E,Sahu B,Yin Y,Wei B,Dodonova SO,Nitta KR,Morgunova E,Taipale M,Cramer P,Taipale J

    更新日期:2018-10-01 00:00:00

  • Water vapour jets inside the plume of gas leaving Enceladus.

    abstract::A plume of water vapour escapes from fissures crossing the south polar region of the Saturnian moon Enceladus. Tidal deformation of a thin surface crust above an internal ocean could result in tensile and compressive stresses that would affect the width of the fissures; therefore, the quantity of water vapour released...


    pub_type: 杂志文章


    authors: Hansen CJ,Esposito LW,Stewart AI,Meinke B,Wallis B,Colwell JE,Hendrix AR,Larsen K,Pryor W,Tian F

    更新日期:2008-11-27 00:00:00

  • Structure of transcribing mammalian RNA polymerase II.

    abstract::RNA polymerase (Pol) II produces messenger RNA during transcription of protein-coding genes in all eukaryotic cells. The Pol II structure is known at high resolution from X-ray crystallography for two yeast species. Structural studies of mammalian Pol II, however, remain limited to low-resolution electron microscopy a...


    pub_type: 杂志文章


    authors: Bernecky C,Herzog F,Baumeister W,Plitzko JM,Cramer P

    更新日期:2016-01-28 00:00:00

  • In situ detection of collisionless reconnection in the Earth's magnetotail.

    abstract::Magnetic reconnection is the process by which magnetic field lines of opposite polarity reconfigure to a lower-energy state, with the release of magnetic energy to the surroundings. Reconnection at the Earth's dayside magnetopause and in the magnetotail allows the solar wind into the magnetosphere. It begins in a smal...


    pub_type: 杂志文章


    authors: Oieroset M,Phan TD,Fujimoto M,Lin RP,Lepping RP

    更新日期:2001-07-26 00:00:00

  • Combined pesticide exposure severely affects individual- and colony-level traits in bees.

    abstract::Reported widespread declines of wild and managed insect pollinators have serious consequences for global ecosystem services and agricultural production. Bees contribute approximately 80% of insect pollination, so it is important to understand and mitigate the causes of current declines in bee populations . Recent stud...


    pub_type: 杂志文章


    authors: Gill RJ,Ramos-Rodriguez O,Raine NE

    更新日期:2012-11-01 00:00:00

  • Dynamics of ribosome scanning and recycling revealed by translation complex profiling.

    abstract::Regulation of messenger RNA translation is central to eukaryotic gene expression control. Regulatory inputs are specified by them RNA untranslated regions (UTRs) and often target translation initiation. Initiation involves binding of the 40S ribosomal small subunit (SSU) and associated eukaryotic initiation factors (e...


    pub_type: 杂志文章


    authors: Archer SK,Shirokikh NE,Beilharz TH,Preiss T

    更新日期:2016-07-28 00:00:00

  • Structural basis for the final steps of human 40S ribosome maturation.

    abstract::Eukaryotic ribosomes consist of a small 40S and a large 60S subunit that are assembled in a highly coordinated manner. More than 200 factors ensure correct modification, processing and folding of ribosomal RNA and the timely incorporation of ribosomal proteins1,2. Small subunit maturation ends in the cytosol, when the...


    pub_type: 杂志文章


    authors: Ameismeier M,Zemp I,van den Heuvel J,Thoms M,Berninghausen O,Kutay U,Beckmann R

    更新日期:2020-11-01 00:00:00

  • Hsp90 as a capacitor for morphological evolution.

    abstract::The heat-shock protein Hsp90 supports diverse but specific signal transducers and lies at the interface of several developmental pathways. We report here that when Drosophila Hsp90 is mutant or pharmacologically impaired, phenotypic variation affecting nearly any adult structure is produced, with specific variants dep...


    pub_type: 杂志文章


    authors: Rutherford SL,Lindquist S

    更新日期:1998-11-26 00:00:00

  • Citrullination regulates pluripotency and histone H1 binding to chromatin.

    abstract::Citrullination is the post-translational conversion of an arginine residue within a protein to the non-coded amino acid citrulline. This modification leads to the loss of a positive charge and reduction in hydrogen-bonding ability. It is carried out by a small family of tissue-specific vertebrate enzymes called peptid...


    pub_type: 杂志文章


    authors: Christophorou MA,Castelo-Branco G,Halley-Stott RP,Oliveira CS,Loos R,Radzisheuskaya A,Mowen KA,Bertone P,Silva JC,Zernicka-Goetz M,Nielsen ML,Gurdon JB,Kouzarides T

    更新日期:2014-03-06 00:00:00