Crystal structure of a yeast TFIIA/TBP/DNA complex.


:The X-ray crystal structure of the transcription factor IIA (TFIIA) in complex with the TATA-box-binding protein (TBP) and TATA-element DNA is presented at 2.5 A resolution. TFIIA is composed of a beta-barrel and a four-helix bundle motif that together have a boot-like appearance. The beta-barrel extends the TBP beta-sheet and bridges over the DNA major groove immediately upstream of the TATA box. The four-helix bundle contributes substantially to the surface of the complex available for interaction with additional transcription factors.






Tan S,Hunziker Y,Sargent DF,Richmond TJ




Has Abstract


1996-05-09 00:00:00














  • The three-dimensional structure of tubulin protofilaments.

    abstract::A three-dimensional image of tubulin protofilaments, reconstructed to 20 A resolution from electron micrographs of negatively stained zinc-induced sheets, has been used to generate an improved model for microtubule substructure. This model has been used to phase a reconstructed image from X-ray amplitudes, which is co...


    pub_type: 杂志文章


    authors: Amos LA,Baker TS

    更新日期:1979-06-14 00:00:00

  • A new route for synthesis of dimethylsulphoniopropionate in marine algae.

    abstract::The 3-dimethylsulphoniopropionate (DMSP) produced by marine algae is the main biogenic precursor of atmospheric dimethylsulphide (DMS). This biogenic DMS, formed by bacterial and algal degradation of DMSP, contributes about 1.5 x 10(13) g of sulphur to the atmosphere annually, and plays a major part in the global sulp...


    pub_type: 杂志文章


    authors: Gage DA,Rhodes D,Nolte KD,Hicks WA,Leustek T,Cooper AJ,Hanson AD

    更新日期:1997-06-26 00:00:00

  • Gfi-1 restricts proliferation and preserves functional integrity of haematopoietic stem cells.

    abstract::Haematopoietic stem cells (HSCs) sustain blood production throughout life. HSCs are capable of extensive proliferative expansion, as a single HSC may reconstitute lethally irradiated hosts. In steady-state, HSCs remain largely quiescent and self-renew at a constant low rate, forestalling their exhaustion during adult ...


    pub_type: 杂志文章


    authors: Hock H,Hamblen MJ,Rooke HM,Schindler JW,Saleque S,Fujiwara Y,Orkin SH

    更新日期:2004-10-21 00:00:00

  • Location-level processes drive the establishment of alien bird populations worldwide.

    abstract::Human-mediated translocation of species to areas beyond their natural distribution (which results in 'alien' populations1) is a key signature of the Anthropocene2, and is a primary global driver of biodiversity loss and environmental change3. Stemming the tide of invasions requires understanding why some species fail ...


    pub_type: 杂志文章


    authors: Redding DW,Pigot AL,Dyer EE,Şekercioğlu ÇH,Kark S,Blackburn TM

    更新日期:2019-07-01 00:00:00

  • Polymer-supported membranes as models of the cell surface.

    abstract::Lipid-bilayer membranes supported on solid substrates are widely used as cell-surface models that connect biological and artificial materials. They can be placed either directly on solids or on ultrathin polymer supports that mimic the generic role of the extracellular matrix. The tools of modern genetic engineering a...


    pub_type: 杂志文章,评审


    authors: Tanaka M,Sackmann E

    更新日期:2005-09-29 00:00:00

  • My hopes for Israel's human-evolution gallery.

    abstract:: ...


    pub_type: 杂志文章


    authors: Hershkovitz I

    更新日期:2019-02-01 00:00:00

  • Happy centenary, photon.

    abstract::One hundred years ago Albert Einstein introduced the concept of the photon. Although in the early years after 1905 the evidence for the quantum nature of light was not compelling, modern experiments--especially those using photon pairs--have beautifully confirmed its corpuscular character. Research on the quantum prop...


    pub_type: 杂志文章


    authors: Zeilinger A,Weihs G,Jennewein T,Aspelmeyer M

    更新日期:2005-01-20 00:00:00

  • Drosophila Stardust is a partner of Crumbs in the control of epithelial cell polarity.

    abstract::The polarized architecture of epithelial cells depends on the highly stereotypic distribution of cellular junctions and other membrane-associated protein complexes. In epithelial cells of the Drosophila embryo, three distinct domains subdivide the lateral plasma membrane. The most apical one comprises the subapical co...


    pub_type: 杂志文章


    authors: Bachmann A,Schneider M,Theilenberg E,Grawe F,Knust E

    更新日期:2001-12-06 00:00:00

  • Intra-tumour diversification in colorectal cancer at the single-cell level.

    abstract::Every cancer originates from a single cell. During expansion of the neoplastic cell population, individual cells acquire genetic and phenotypic differences from each other. Here, to investigate the nature and extent of intra-tumour diversification, we characterized organoids derived from multiple single cells from thr...


    pub_type: 杂志文章


    authors: Roerink SF,Sasaki N,Lee-Six H,Young MD,Alexandrov LB,Behjati S,Mitchell TJ,Grossmann S,Lightfoot H,Egan DA,Pronk A,Smakman N,van Gorp J,Anderson E,Gamble SJ,Alder C,van de Wetering M,Campbell PJ,Stratton MR,Clevers

    更新日期:2018-04-01 00:00:00

  • A prevalence of dynamo-generated magnetic fields in the cores of intermediate-mass stars.

    abstract::Magnetic fields play a part in almost all stages of stellar evolution. Most low-mass stars, including the Sun, show surface fields that are generated by dynamo processes in their convective envelopes. Intermediate-mass stars do not have deep convective envelopes, although 10 per cent exhibit strong surface fields that...


    pub_type: 杂志文章


    authors: Stello D,Cantiello M,Fuller J,Huber D,García RA,Bedding TR,Bildsten L,Aguirre VS

    更新日期:2016-01-21 00:00:00

  • Inhibition of suppressor T-cell development following deoxyguanosine administration.

    abstract::The expression of immunodeficiency in patients with specific purine enzyme defects indicates a crucial role of the purine salvage pathway in the acquisition and expression of normal immune function. One current hypothesis links the failure of normal lymphocyte development in these diseases to the accumulation of deoxy...


    pub_type: 杂志文章


    authors: Dosch HM,Mansour A,Cohen A,Shore A,Gelfand EW

    更新日期:1980-06-12 00:00:00

  • A molecular timescale for vertebrate evolution.

    abstract::A timescale is necessary for estimating rates of molecular and morphological change in organisms and for interpreting patterns of macroevolution and biogeography. Traditionally, these times have been obtained from the fossil record, where the earliest representatives of two lineages establish a minimum time of diverge...


    pub_type: 杂志文章


    authors: Kumar S,Hedges SB

    更新日期:1998-04-30 00:00:00

  • Hypothalamic programming of systemic ageing involving IKK-β, NF-κB and GnRH.

    abstract::Ageing is a result of gradual and overall functional deteriorations across the body; however, it is unknown whether an individual tissue primarily works to mediate the ageing progress and control lifespan. Here we show that the hypothalamus is important for the development of whole-body ageing in mice, and that the un...


    pub_type: 杂志文章


    authors: Zhang G,Li J,Purkayastha S,Tang Y,Zhang H,Yin Y,Li B,Liu G,Cai D

    更新日期:2013-05-09 00:00:00

  • Forensics: age written in teeth by nuclear tests.

    abstract::Establishing the age at death of individuals is an important step in their identification and can be done with high precision up to adolescence by analysis of dentition, but it is more difficult in adults. Here we show that the amount of radiocarbon present in tooth enamel as a result of nuclear bomb testing during 19...


    pub_type: 杂志文章


    authors: Spalding KL,Buchholz BA,Bergman LE,Druid H,Frisén J

    更新日期:2005-09-15 00:00:00

  • Requirement for monocytes in the spontaneous cytotoxic effects of human lymphocytes against non-lymphoid target cells.

    abstract::Human lymphocytes have been shown to lyse in vitro a large variety of target cells derived from tumour as well as normal tissues. The effector cells involved in this spontaneous cell-mediated cytotoxicity (SCMC) have generally been characterised as lymphocytes which lack surface membrane immunoglobulin (sIg) and lack ...


    pub_type: 杂志文章


    authors: de Vries JE,Mendelsohn J,Bont WS

    更新日期:1980-02-07 00:00:00

  • Chimpanzees and AIDS research.

    abstract::Pressure is mounting to relax the regulations on importation of chimpanzees for research. Such a policy is unnecessary and would deepen the plight of an already endangered species. ...


    pub_type: 杂志文章


    authors: Prince AM,Moor-Jankowski J,Eichberg JW,Schellekens H,Mauler RF,Girard M,Goodall J

    更新日期:1988-06-09 00:00:00

  • Surface topography dependence of biomolecular hydrophobic hydration.

    abstract::Many biomolecules are characterized by surfaces containing extended nonpolar regions, and the aggregation and subsequent removal of such surfaces from water is believed to play a critical role in the biomolecular assembly in cells. A better understanding of the hydrophobic hydration of biomolecules may therefore yield...


    pub_type: 杂志文章


    authors: Cheng YK,Rossky PJ

    更新日期:1998-04-16 00:00:00

  • Wild-type p53 induces apoptosis of myeloid leukaemic cells that is inhibited by interleukin-6.

    abstract::Wild-type p53 protein has many properties consistent with its being the product of a tumour suppressor gene. Although the normal roles of tumour suppressor genes are still largely unknown, it seems that they could be involved in promoting cell differentiation as well as in mediating growth arrest by growth-inhibitory ...


    pub_type: 杂志文章


    authors: Yonish-Rouach E,Resnitzky D,Lotem J,Sachs L,Kimchi A,Oren M

    更新日期:1991-07-25 00:00:00

  • Quadrupole transitions revealed by Borrmann spectroscopy.

    abstract::The Borrmann effect-a dramatic increase in transparency to X-ray beams-is observed when X-rays satisfying Bragg's law diffract through a perfect crystal. The minimization of absorption seen in the Borrmann effect has been explained by noting that the electric field of the X-ray beam approaches zero amplitude at the cr...


    pub_type: 杂志文章


    authors: Pettifer RF,Collins SP,Laundy D

    更新日期:2008-07-10 00:00:00

  • Commensal microbe-derived butyrate induces the differentiation of colonic regulatory T cells.

    abstract::Gut commensal microbes shape the mucosal immune system by regulating the differentiation and expansion of several types of T cell. Clostridia, a dominant class of commensal microbe, can induce colonic regulatory T (Treg) cells, which have a central role in the suppression of inflammatory and allergic responses. Howeve...


    pub_type: 杂志文章


    authors: Furusawa Y,Obata Y,Fukuda S,Endo TA,Nakato G,Takahashi D,Nakanishi Y,Uetake C,Kato K,Kato T,Takahashi M,Fukuda NN,Murakami S,Miyauchi E,Hino S,Atarashi K,Onawa S,Fujimura Y,Lockett T,Clarke JM,Topping DL,Tomita

    更新日期:2013-12-19 00:00:00

  • A black-hole mass measurement from molecular gas kinematics in NGC4526.

    abstract::The masses of the supermassive black holes found in galaxy bulges are correlated with a multitude of galaxy properties, leading to suggestions that galaxies and black holes may evolve together. The number of reliably measured black-hole masses is small, and the number of methods for measuring them is limited, holding ...


    pub_type: 杂志文章


    authors: Davis TA,Bureau M,Cappellari M,Sarzi M,Blitz L

    更新日期:2013-02-21 00:00:00

  • The t(15;17) translocation of acute promyelocytic leukaemia fuses the retinoic acid receptor alpha gene to a novel transcribed locus.

    abstract::Retinoic acid is a vitamin A derivative with striking effects on development and cell differentiation. Several nuclear retinoic acid receptors (RARs), acting as ligand-inducible transcription factors, have been characterized and indirect evidence suggests that they have distinct roles. One of the most intriguing prope...


    pub_type: 杂志文章


    authors: de Thé H,Chomienne C,Lanotte M,Degos L,Dejean A

    更新日期:1990-10-11 00:00:00

  • Paramutation in Drosophila linked to emergence of a piRNA-producing locus.

    abstract::A paramutation is an epigenetic interaction between two alleles of a locus, through which one allele induces a heritable modification in the other allele without modifying the DNA sequence. The paramutated allele itself becomes paramutagenic, that is, capable of epigenetically converting a new paramutable allele. Here...


    pub_type: 杂志文章


    authors: de Vanssay A,Bougé AL,Boivin A,Hermant C,Teysset L,Delmarre V,Antoniewski C,Ronsseray S

    更新日期:2012-10-04 00:00:00

  • A hexagonal planar transition-metal complex.

    abstract::Transition-metal complexes are widely used in the physical and biological sciences. They have essential roles in catalysis, synthesis, materials science, photophysics and bioinorganic chemistry. Our understanding of transition-metal complexes originates from Alfred Werner's realization that their three-dimensional sha...


    pub_type: 杂志文章


    authors: Garçon M,Bakewell C,Sackman GA,White AJP,Cooper RI,Edwards AJ,Crimmin MR

    更新日期:2019-10-01 00:00:00

  • Two-dimensional gas of massless Dirac fermions in graphene.

    abstract::Quantum electrodynamics (resulting from the merger of quantum mechanics and relativity theory) has provided a clear understanding of phenomena ranging from particle physics to cosmology and from astrophysics to quantum chemistry. The ideas underlying quantum electrodynamics also influence the theory of condensed matte...


    pub_type: 杂志文章


    authors: Novoselov KS,Geim AK,Morozov SV,Jiang D,Katsnelson MI,Grigorieva IV,Dubonos SV,Firsov AA

    更新日期:2005-11-10 00:00:00

  • The role of interfacial lipids in stabilizing membrane protein oligomers.

    abstract::Oligomerization of membrane proteins in response to lipid binding has a critical role in many cell-signalling pathways but is often difficult to define or predict. Here we report the development of a mass spectrometry platform to determine simultaneously the presence of interfacial lipids and oligomeric stability and ...


    pub_type: 杂志文章


    authors: Gupta K,Donlan JAC,Hopper JTS,Uzdavinys P,Landreh M,Struwe WB,Drew D,Baldwin AJ,Stansfeld PJ,Robinson CV

    更新日期:2017-01-19 00:00:00

  • Cell biology: nondisjunction, aneuploidy and tetraploidy.

    abstract::One simple, widely accepted mechanism for generating an aberrant chromosome number, or aneuploidy, is through nondisjunction--a chromosome distribution error that occurs during mitosis when both copies of a duplicated chromosome are deposited into one daughter cell and none into the other. Shi and King challenge this ...


    pub_type: 评论,杂志文章


    authors: Weaver BA,Silk AD,Cleveland DW

    更新日期:2006-08-17 00:00:00

  • The architecture of respiratory complex I.

    abstract::Complex I is the first enzyme of the respiratory chain and has a central role in cellular energy production, coupling electron transfer between NADH and quinone to proton translocation by an unknown mechanism. Dysfunction of complex I has been implicated in many human neurodegenerative diseases. We have determined the...


    pub_type: 杂志文章


    authors: Efremov RG,Baradaran R,Sazanov LA

    更新日期:2010-05-27 00:00:00

  • Possible presence of high-pressure ice in cold subducting slabs.

    abstract::During the subduction of oceanic lithosphere, water is liberated from minerals by progressive dehydration reactions and is thought to be critical to several geologically important processes such as island-arc volcanism, intermediate-depth seismicity and chemical exchange between the subducting lithosphere and mantle. ...


    pub_type: 杂志文章


    authors: Bina CR,Navrotsky A

    更新日期:2000-12-14 00:00:00

  • Adenylyl cyclases and the interaction between calcium and cAMP signalling.

    abstract::Adenylyl cyclase is the prototypical second messenger generator. Nearly all of the eight cloned adenylyl cyclases are regulated by one or other arm of the phospholipase C pathway. Functional and ultrastructural investigations have shown that adenylyl cyclases are intimately associated with sites of calcium ion entry i...


    pub_type: 杂志文章,评审


    authors: Cooper DM,Mons N,Karpen JW

    更新日期:1995-03-30 00:00:00