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Factors affecting sperm motility. IV. Incubation of human semen with caffeine, kallikrein, and other metabolically active compounds.

Abstract：

:The effect of six metabolically active compounds, namely caffeine, kallikrein, cyclic adenosine 3':5'-monophosphate, prostaglandin E, triiodothyronine, and insulin on spermatozoal motility was investigated. Hypokinetic and normokinetic semen specimens from fertile and infertile patients were incubated with various concentrations of these compounds. Spermatozoal velocity and percentage of motility were determined periodically with the objective multiple exposure photography (MEP) method before and during the following 4 to 8 hours. No significant differences was found between experimental and control specimens as to these parameters under the influence of the tested compounds, with the exception of caffeine. Caffeine immediately increased the percentage of motile spermatozoa by 30% to 50% of initial values in approximately two-thirds of cases. No influence on sperm velocity was detected during this period. By using the combined MEP and supravital staining techniques it was found that nonmotile live spermatozoa were activated by caffeine without any effect on the percentage of dead sperm. Some practical implications of these findings, the difference between our results and those reported by others, and the mechanism of action of these compounds are reviewed and discussed. :A study was conducted to evaluate the effect of various compounds on the motility of both hypokinetic and normokinetic spermatozoa. The 6 metabolically active substances used in the study were: caffeine, kallikrein, cyclic adenosine 3':5'-monophosphate, prostaglandin E, triiodothyronine, and insulin. Sperm specimens were taken from both fertile and infertile men. MEP (multiple exposure photography) was used to assess spermatozoal velocity and percentage of motility before introduction of the substances and at 4 and 8 hours post usage. Mean values of spermaozoal velocity and percentage of motility in specimens incubated with the diffeent compounds and various controls are tabulated. Only caffeine induced a significant stimulatory effect on sperm motility; the effect started immediately and lasted for about 4 hours. The relative increase of motility was on the order of 40%. There was no effect on sperm velocity. Prostaglandin E had a mild but insignificant stimulatory effect on sperm motility, but none of the other compounds had any stimulatory or depressant effect. Before caffeine can be added to hypokinetic fresh or frozen sperm for insemination, the possibility of its teratogenicity must be considered.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Makler A,Makler E,Itzkovitz J,Brandes JM

subject

Has Abstract

pub_date

1980-06-01 00:00:00

pages

624-30

issue

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)44776-X

journal_volume

pub_type

杂志文章

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Factors affecting sperm motility. IV. Incubation of human semen with caffeine, kallikrein, and other metabolically active compounds.