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Noninvasive metabolomic profiling of embryo culture media using proton nuclear magnetic resonance correlates with reproductive potential of embryos in women undergoing in vitro fertilization.

Abstract：

OBJECTIVE:To identify biomarkers associated with reproductive outcome using proton nuclear magnetic resonance ((1)H NMR) metabolomic profiling of embryo culture media. DESIGN:Retrospective study. SETTING:An academic assisted reproductive technology (ART) program; a university research center. PATIENT(S):Women undergoing ART treatment. INTERVENTION(S):Spent media samples from embryos that resulted in pregnancy and delivery (n = 17) and samples (n = 17) from embryos that failed to implant were individually collected on day 3, and evaluated using (1)H NMR spectroscopy. The spectra obtained were quantified by integrating six biomarker signals in the aliphatic region after baseline subtraction. Using a multivariate analysis, a model that calculates a viability index for each spectrum was developed. Sensitivity and specificity of predicting pregnancy (described as implantation and delivery) were calculated. MAIN OUTCOME MEASURE(S):The (1)H NMR metabolomic profile of embryo culture media and embryo viability. RESULT(S):Glutamate concentrations determined by (1)H NMR were significantly higher in spent culture media of embryos that resulted in pregnancy and delivery compared to those that failed to implant. Similarly, viability indices calculated by (1)H NMR using the weighted coefficients of glutamate and alanine/lactate ratio quantities were higher for embryos that implanted and resulted in a delivery. Proton NMR spectroscopy predicted viability of individual embryos with a sensitivity of 88.2% and a specificity of 88.2%. CONCLUSION(S):Metabolomic profile of spent embryo culture media using (1)H NMR correlates with the reproductive potential of embryos.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Seli E,Botros L,Sakkas D,Burns DH

doi

10.1016/j.fertnstert.2008.07.1739

subject

Has Abstract

pub_date

2008-12-01 00:00:00

pages

2183-9

issue

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(08)03264-0

journal_volume

pub_type

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Noninvasive metabolomic profiling of embryo culture media using proton nuclear magnetic resonance correlates with reproductive potential of embryos in women undergoing in vitro fertilization.