The role of the laboratory in the handling of epididymal sperm for assisted reproductive technologies.

Abstract:

:This report describes the historical experience of our laboratory in the handling and processing of epididymal sperm from men with congenital absence of the vas deferens. Three different methods of sperm preparation were used: swim-up or resuspension in the first group of patients treated, mini-Percoll in the second group, and mini-Percoll used in conjunction with pentoxifylline and 2' deoxyadenosine in the third group. Twenty-four percent of cases achieved fertilization in group I, whereas in groups II and III the rate was 59% and 57%, respectively. Fertilization rates in each group were 6.5% in group I, 16% in group II, and 35% in group III. Three pregnancies were obtained in group I, 13 in group II, and 2 in group III. Groups II (21%) and III (63%) were able to have excess embryos to cryopreserve.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Ord T,Marello E,Patrizio P,Balmaceda JP,Silber SJ,Asch RH

doi

10.1016/s0015-0282(16)55031-6

keywords:

subject

Has Abstract

pub_date

1992-05-01 00:00:00

pages

1103-6

issue

5

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)55031-6

journal_volume

57

pub_type

杂志文章
  • Immunological aspects of surgically induced experimental endometriosis: variation in response to therapy.

    abstract::Elevated endometrial antibody titers were detected in serum and peritoneal fluid of rabbits with experimentally induced endometriosis. Surgical extirpation of implants or suppression with gonadotropin-releasing hormone agonist resulted in decreased endometrial antibody titers, whereas the antibody titers in untreated ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/s0015-0282(16)60802-6

    authors: Homm RJ,Garza DE,Mathur S,Austin M,Baggett B,Williamson HO

    更新日期:1989-07-01 00:00:00

  • DAZL protein expression in mouse preimplantation embryo.

    abstract:OBJECTIVE:To evaluate the expression pattern of Dazl (deleted in azoospermia-like) protein in the mouse preimplantation embryo. DESIGN:Experimental study. SETTING:Medical research laboratory in a university hospital. ANIMAL(S):Twenty female 28- to 35-day-old FVB mice. INTERVENTION(S):Embryo collection at 1.5, 2.5, ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2007.03.041

    authors: Pan HA,Liao RW,Chung CL,Teng YN,Lin YM,Kuo PL

    更新日期:2008-05-01 00:00:00

  • The development of cytotoxicity in peritoneal macrophages from women with endometriosis.

    abstract:OBJECTIVE:To assess the activation status of peritoneal macrophages from women with endometriosis. DESIGN:Peritoneal macrophages from patients undergoing laparoscopy were tested for cytotoxic activity against a cultured hepatoma cell line. SETTING:Patients were tested at initial laparoscopy or at the completion of th...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:

    authors: Braun DP,Gebel H,Rotman C,Rana N,Dmowski WP

    更新日期:1992-06-01 00:00:00

  • Estrogen raises the sweating threshold in postmenopausal women with hot flashes.

    abstract:OBJECTIVE:To determine if estrogen ameliorates hot flashes by raising the core body temperature sweating threshold, by reducing core body temperature fluctuations, and/or by reducing sympathetic activation (as measured by plasma 3-methoxy-4-hydroxyphenylglycol). DESIGN:Laboratory physiological study. SETTING:Universi...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1016/s0015-0282(01)03009-6

    authors: Freedman RR,Blacker CM

    更新日期:2002-03-01 00:00:00

  • Molecular aspects of declining sperm motility in older men.

    abstract:OBJECTIVE:To investigate the molecular mechanism for declining sperm motility in older men. DESIGN:Retrospective and prospective study. SETTING:University-based andrology unit. PATIENT(S):Semen was collected from 2,111 patients and sperm donors. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Age, sperm concentratio...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2005.05.020

    authors: Henkel R,Maass G,Schuppe HC,Jung A,Schubert J,Schill WB

    更新日期:2005-11-01 00:00:00

  • Hyperprolactinemic male infertility.

    abstract::Serum prolactin was studied in 25 fertile and 127 infertile men. The latter included 91 oligospermic, 27 azoospermic, and 9 hypogonadotropic hypogonadal men. The mean prolactin level in all three groups of infertile men was significantly above that of the fertile group. There was no correlation among serum levels of f...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/s0015-0282(16)42259-4

    authors: Segal S,Polishuk WZ,Ben-David M

    更新日期:1976-12-01 00:00:00

  • Blastocyst formation, pregnancy, and birth derived from human oocytes cryopreserved for 5 years.

    abstract:OBJECTIVE:To report a live birth after the transfer of a single blastocyst derived from a human oocyte cryopreserved for 5 years. DESIGN:Case report. SETTING:Private assisted reproduction center. PATIENT(S):A 39-year-old woman with tubal infertility and her partner with male-related infertility. INTERVENTION(S):Ooc...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2008.01.106

    authors: Parmegiani L,Fabbri R,Cognigni GE,Bernardi S,Pocognoli P,Filicori M

    更新日期:2008-11-01 00:00:00

  • Treatment with gonadotropin-releasing hormone (GnRH) antagonists in women suppressed with GnRH agonist may avoid cycle cancellation in patients at risk for ovarian hyperstimulation syndrome.

    abstract::Forty-seven patients at high risk for ovarian hyperstimulation syndrome because of markedly elevated serum E2 levels on either long-luteal or microdose flare leuprolide acetate regimens were treated with ganirelix acetate. Despite being pretreated with GnRH agonist and without withholding gonadotropins, serum E2 decre...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2005.07.1291

    authors: Gustofson RL,Larsen FW,Bush MR,Segars JH

    更新日期:2006-01-01 00:00:00

  • Clinical evaluation of the effects of combined treatment with bromocriptine and spironolactone in two women with the polycystic ovary syndrome.

    abstract::Two women with the polycystic ovary syndrome were treated with bromocriptine (15 to 20 mg/day) in combination with spironolactone (100 mg/day). In the first woman the combined therapy induced a marked reduction of hirsutism, with ensuing ovulation and pregnancy. In the second woman, who had hyperthecosis and had been ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/s0015-0282(16)45554-8

    authors: Blum I,Bruhis S,Kaufman H

    更新日期:1981-06-01 00:00:00

  • Evidence for a Müllerian-inhibiting substance autocrine/paracrine system in adult human endometrium.

    abstract:OBJECTIVE:To determine if adult human endometrium possesses an intact müllerian-inhibiting substance (MIS) signal transduction system and, if so, whether MIS can modulate endometrial cell viability. DESIGN:Adult human endometrial tissue was subjected to reverse transcription polymerase chain reaction (RT-PCR) analysis...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2008.01.028

    authors: Wang J,Dicken C,Lustbader JW,Tortoriello DV

    更新日期:2009-04-01 00:00:00

  • Inducible nitric oxide synthase expression by peritoneal macrophages in endometriosis-associated infertility.

    abstract:OBJECTIVE:Determine whether peritoneal macrophages from women with endometriosis-associated infertility express more inducible nitric oxide synthase (NOS2) and produce more NO than fertile controls. DESIGN:Unblinded clinical study. PATIENT(S):Nine infertile women with endometriosis and nine normal fertile women under...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/s0015-0282(01)02940-5

    authors: Osborn BH,Haney AF,Misukonis MA,Weinberg JB

    更新日期:2002-01-01 00:00:00

  • Transmyometrial embryo transfer after difficult immediate mock transcervical transfer.

    abstract:OBJECTIVE:To evaluate the place of ultrasound-directed transvaginal transmyometrial ET in a protocol using both the transcervical and transmyometrial routes in a step-wise fashion. DESIGN:A prospective descriptive clinical study. SETTING:A university-based assisted conception unit. PATIENTS:Thirteen patients who had...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:

    authors: Sharif K,Afnan M,Lenton W,Bilalis D,Hunjan M,Khalaf Y

    更新日期:1996-05-01 00:00:00

  • Detection of the ovulatory-luteinizing hormone surge with an enzyme-linked immunospecific human urinary luteinizing hormone assay: applicability to nonhuman primates.

    abstract::Heterologous antibodies have frequently been used to develop assays in other species. Human urinary LH kits, widely available for predicting the LH surge, offer a unique opportunity to determine the timing of ovulation and mating in nonhuman primates. We tested three commercially available ovulation predictor kits for...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/s0015-0282(16)53301-9

    authors: Simon JA,Esparcia-Fenwick LA,Hodgen GD

    更新日期:1990-02-01 00:00:00

  • Expression of platelet endothelial cell adhesion molecule-1 in red and black endometriotic lesions.

    abstract::This immunohistochemical study shows that platelet endothelial cell adhesion molecule-1 (PECAM-1), known to play a role in neoangiogenesis and vascular development, is strongly and homogeneously expressed in endothelial cells lining blood vessels from red and black pelvic endometriotic lesions. The distribution of PEC...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2004.05.075

    authors: Van Langendonckt A,Eggermont J,Casanas-Roux F,Scholtes HE,Donnez J

    更新日期:2004-10-01 00:00:00

  • Follicle-stimulating hormone or human menopausal gonadotropin for ovarian stimulation in in vitro fertilization cycles: a meta-analysis.

    abstract:OBJECTIVE:To reanalyze the results of using FSH alone and hMG during IVF treatment, taking into account the different protocols of administration of superactive GnRH agonist analogs. DESIGN:Meta-analysis. SETTING:The London Women's Clinic. PATIENT(S):Women undergoing IVF treatment. INTERVENTION(S):A meta-analysis o...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,meta分析

    doi:10.1016/s0015-0282(99)00519-1

    authors: Agrawal R,Holmes J,Jacobs HS

    更新日期:2000-02-01 00:00:00

  • Treatment of uterine leiomyomata with a luteinizing hormone-releasing hormone agonist: the possibility of nonsurgical management in selected perimenopausal women.

    abstract:OBJECTIVE:To evaluate the efficacy of luteinizing hormone-releasing hormone agonist (LH-RH-a) in the treatment of leiomyomata. DESIGN:A retrospective randomized trial. SETTING:Hospital department of obstetrics and gynecology. PATIENTS:Twenty-five women, ages 36 to 54 years with symptomatic uterine leiomyomata, were ...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章

    doi:10.1016/s0015-0282(16)54296-4

    authors: Nakamura Y,Yoshimura Y,Yamada H,Ubukata Y,Ando M,Suzuki M

    更新日期:1991-05-01 00:00:00

  • Postdelivery adjustment of gestational carriers, intended parents, and their children.

    abstract::Surrogacy raises a number of concerns about the psychological adjustment of the surrogate, the parents, and the child. Despite surrogacy becoming more common, research into the postdelivery psychological adjustment of the individuals involved has lagged far behind. This review examines research on the psychological ad...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审

    doi:10.1016/j.fertnstert.2020.03.010

    authors: Jadva V

    更新日期:2020-05-01 00:00:00

  • Studies of gene expression in human cumulus cells indicate pentraxin 3 as a possible marker for oocyte quality.

    abstract:OBJECTIVE:To search for differentially expressed genes in cumulus cells from two groups of oocytes with different developmental outcome in vitro. DESIGN:Analyses of gene expression in human cumulus cells from oocytes that failed to fertilize in vitro (group A) and those that developed into normal-appearing embryos on ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2004.11.030

    authors: Zhang X,Jafari N,Barnes RB,Confino E,Milad M,Kazer RR

    更新日期:2005-04-01 00:00:00

  • Medroxyprogesterone acetate is an effective oral alternative for preventing premature luteinizing hormone surges in women undergoing controlled ovarian hyperstimulation for in vitro fertilization.

    abstract:OBJECTIVE:To investigate the use of medroxyprogesterone acetate (MPA) to prevent LH surge during controlled ovarian hyperstimulation (COH) and to compare cycle characteristics and pregnancy outcomes in subsequently frozen-thawed ET (FET) cycles. DESIGN:A prospective controlled study. SETTING:Tertiary-care academic me...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2015.03.022

    authors: Kuang Y,Chen Q,Fu Y,Wang Y,Hong Q,Lyu Q,Ai A,Shoham Z

    更新日期:2015-07-01 00:00:00

  • The technique for human embryo transfer.

    abstract::In a program of human in vitro fertilization (IVF), the results of 204 attempted intracervical embryo transfers (ETs), using a variety of catheters in three trials over 18 months, have been analyzed for the ease of transfer and pregnancy rate. In nulliparous patients, transfers were more difficult than in multiparous ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/s0015-0282(16)46451-4

    authors: Leeton J,Trounson A,Jessup D,Wood C

    更新日期:1982-08-01 00:00:00

  • Effects of tibolone and continuous combined hormone replacement therapy on parameters in the clotting cascade: a multicenter, double-blind, randomized study.

    abstract:OBJECTIVE:To determine the effects of tibolone and continuous combined HRT (ccHRT) on parameters in the clotting cascade. DESIGN:Randomized, double-blind study. SETTING:Hemostasis unit of a university hospital clinic in Germany. PATIENT(S):Sixty healthy postmenopausal women. INTERVENTION(S):Twenty-nine subjects wer...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:10.1016/s0015-0282(00)00587-2

    authors: Winkler UH,Altkemper R,Kwee B,Helmond FA,Coelingh Bennink HJ

    更新日期:2000-07-01 00:00:00

  • Effects of exposure to high levels of particulate air pollution during the follicular phase of the conception cycle on pregnancy outcome in couples undergoing in vitro fertilization and embryo transfer.

    abstract::The objective of this retrospective cohort study was to assess the potential effects of preconceptional short-term exposure to particulate air pollution in a real-world situation on pregnancy outcome in infertile women evaluating the possible role of IVF/embryo transfer treatment on this outcome using women who had co...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2009.06.031

    authors: Perin PM,Maluf M,Czeresnia CE,Nicolosi Foltran Januário DA,Nascimento Saldiva PH

    更新日期:2010-01-01 00:00:00

  • Paradoxical effects of D-Trp6-luteinizing hormone-releasing hormone on the hypothalamic-pituitary-gonadal axis in immature female rats.

    abstract::The effect of administration of a superactive and long-acting analog of luteinizing hormone-releasing hormone (LH-RH), D-Trp6-LH-RH, in doses of 0.05 or 1 microgram/day for 10 days on the hypothalamic-pituitary-gonadal axis was studied in immature female rats. Treatment with a 0.05-microgram dose of analog produced fe...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/s0015-0282(16)44061-6

    authors: Vilchez-Martinez JA,Pedroza E,Arimura A,Schally AV

    更新日期:1979-06-01 00:00:00

  • Provision of fertility services for women at increased risk of complications during fertility treatment or pregnancy: an Ethics Committee opinion.

    abstract::This opinion addresses the ethics of providing fertility treatment to women at elevated risk from fertility treatment or pregnancy. Providers ethically may treat women at elevated risk provided that they are carefully assessed; that specialists in their medical condition are consulted as appropriate; and that patients...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,实务指引

    doi:10.1016/j.fertnstert.2016.08.015

    authors: Ethics Committee of the American Society for Reproductive Medicine. Electronic address: ASRM@asrm.org.,Ethics Committee of the American Society for Reproductive Medicine.

    更新日期:2016-11-01 00:00:00

  • Image in reproductive medicine. Cesarean scar ectopic pregnancy.

    abstract::Bilateral hypogastric artery ligation followed by dilatation and evacuation under laparoscopic guidance was successful in the treatment of an advanced cesarean scar ectopic pregnancy. This case presents images of ultrasound, magnetic resonance imaging, and gross anatomy unique to cesarean scar pregnancy. ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2005.12.016

    authors: Kung FT,Huang TL,Chen CW,Cheng YF

    更新日期:2006-05-01 00:00:00

  • Cyclic changes in the concentration of glucose and fructose in human cervical mucus.

    abstract:OBJECTIVE:To determine a possible cyclic change in the concentration of glucose and fructose in the aqueous phase of human cervical mucus (CM). DESIGN:Concentrations of glucose and fructose were longitudinally determined in the aqueous phase of CM of normal cycling women using enzymatic techniques, modified for small ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/s0015-0282(16)54902-4

    authors: van der Linden PJ,Kets M,Gimpel JA,Wiegerinck MA

    更新日期:1992-03-01 00:00:00

  • Regulated upon activation, normal T cell expressed and secreted (RANTES) and monocyte chemotactic protein 1 in follicular fluid accumulate differentially in patients with and without endometriosis undergoing in vitro fertilization.

    abstract:OBJECTIVE:To compare the amount of regulated upon activation, normal T cell expressed and secreted (RANTES) and monocyte chemotactic protein 1 (MCP-1) in follicular fluid (FF) of patients with and without endometriosis and to determine their oocyte fertilization and pregnancy rates. DESIGN:Case-control study. SETTING...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2006.05.043

    authors: Xu H,Schultze-Mosgau A,Agic A,Diedrich K,Taylor RN,Hornung D

    更新日期:2006-12-01 00:00:00

  • Embryo disposition: choices made by patients and donor oocyte recipients.

    abstract:OBJECTIVE:To compare final embryo disposition between patients and donor oocyte recipients. DESIGN:Retrospective study. SETTING:Private infertility practice. PATIENT(S):Patients undergoing IVF with embryo cryopreservation. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Final cryopreserved embryo disposition. RESUL...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2010.08.002

    authors: Hill GA,Freeman MR

    更新日期:2011-03-01 00:00:00

  • Reduction of epididymal sperm motility after ablation of the inferior mesenteric plexus in the rat.

    abstract::Surgical ablation of the inferior mesenteric plexus in the rat results in an excessive accumulation of sperm within the cauda epididymidis. The present study examined the effect of ablation of the inferior mesenteric plexus on the motility of cauda epididymal spermatozoa. One to 8 weeks after ablation of the inferior ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:

    authors: Billups KL,Tillman SL,Chang TS

    更新日期:1990-06-01 00:00:00

  • In vitro culture and in vitro maturation of mouse preantral follicles with recombinant gonadotropins.

    abstract:OBJECTIVE:To develop an effective method for in vitro maturation of preantral follicles isolated from mice ovarian tissue. DESIGN:Isolated preantral follicles were randomly allocated to designed experimental groups for study. SETTING:University-based research lab. PATIENT(S):Healthy, normal mice. INTERVENTION(S):Su...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/s0015-0282(01)02977-6

    authors: Liu HC,He Z,Rosenwaks Z

    更新日期:2002-02-01 00:00:00