Nuclear magnetic resonance spectroscopy: 13C spectra of some common nucleotides.

abstract：:In a significant fraction of the Escherichia coli cytosolic proteins, the N-terminal methionine residue incorporated during the translation initiation step is excised. The N-terminal methionine excision is catalyzed by methionyl-aminopeptidase (MAP). Previous studies have suggested that the action of this enzyme could...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirel PH,Schmitter MJ,Dessen P,Fayat G,Blanquet S

更新日期：1989-11-01 00:00:00

abstract：:A single catalytic site model is proposed to account for the multiphasic kinetics of oxidation of ferrocytochrome c by cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase, EC 1.9.3.1). This model involves nonproductive binding of substrate to sites near the catalytic site on cytochrome c oxidase for cytochro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Speck SH,Dye D,Margoliash E

更新日期：1984-01-01 00:00:00

abstract：:We present here a brief review of direct force measurements between hydrophobic surfaces in aqueous solutions. For almost 70 years, researchers have attempted to understand the hydrophobic effect (the low solubility of hydrophobic solutes in water) and the hydrophobic interaction or force (the unusually strong attract...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meyer EE,Rosenberg KJ,Israelachvili J

更新日期：2006-10-24 00:00:00

abstract：:Whole-genome duplication (WGD) is believed to be a significant source of major evolutionary innovation. Redundant genes resulting from WGD are thought to be lost or acquire new functions. However, the rates of gene loss and thus temporal process of genome reshaping after WGD remain unclear. The WGD shared by all teleo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Inoue J,Sato Y,Sinclair R,Tsukamoto K,Nishida M

更新日期：2015-12-01 00:00:00

abstract：:We show that endemic (eBL), sporadic (sBL), and acquired immunodeficiency syndrome-associated (AIDS-BL) forms of Burkitt lymphoma (BL) carrying t(8;14) chromosomal translocations display different breakpoints within the immunoglobulin heavy-chain locus (IGH) on chromosome 14. In sBL (7 out of 11) and AIDS-BL (5 out of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neri A,Barriga F,Knowles DM,Magrath IT,Dalla-Favera R

更新日期：1988-04-01 00:00:00

abstract：:We have located a positive, cis-acting DNA sequence element within the 5' flanking DNA of the c-myc gene (-125 base pairs). This DNA sequence element has a large purine-pyrimidine strand asymmetry and can assume the H-DNA conformation. A factor with the properties of a ribonucleoprotein (RNP) interacts with this DNA r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davis TL,Firulli AB,Kinniburgh AJ

更新日期：1989-12-01 00:00:00

abstract：:The exo isomer of aflatoxin B1 (AFB1) 8,9-epoxide appears to be the only product of AFB1 involved in reaction with DNA and reacts with the N7 atom of guanine via an SN2 reaction from an intercalated state. Although the epoxide hydrolyzes rapidly in H2O (0.6 s-1 at 25 degrees C), very high yields of DNA adduct result. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Johnson WW,Guengerich FP

更新日期：1997-06-10 00:00:00

abstract：:Reducible compounds that participate in crosslinking were analyzed in hydroxylysine-deficient collagens of patients with a heritable disorder of connective tissue. After treatment with [(3)H]sodium borohydride, new compounds, as well as a totally different pattern of tritiated compounds, were found in hydroxylysine-de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eyre DR,Glimcher MJ

更新日期：1972-09-01 00:00:00

abstract：:The emerging picture of Earth's deep interior from seismic tomography indicates more complexity than previously thought. The presence of lateral anisotropy and heterogeneity in Earth's mantle highlights the need for fully anisotropic elasticity data from mineral physics. A breakthrough in high-frequency (gigahertz) ul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jacobsen SD,Spetzler H,Reichmann HJ,Smyth JR

更新日期：2004-04-20 00:00:00

abstract：:To study the origin of and the degree of T-cell antigen receptor (TCR) diversity of Thy-1+ dendritic epidermal cells (Thy-1+ dECs) in mice, we have developed a monoclonal antibody (mAb 536) to the gamma delta TCR. mAb 536 binds to and stimulates interleukin 2 secretion from Thy-1+ dEC but not cells that express TCR co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Havran WL,Grell S,Duwe G,Kimura J,Wilson A,Kruisbeek AM,O'Brien RL,Born W,Tigelaar RE,Allison JP

更新日期：1989-06-01 00:00:00

abstract：:Redistribution of specialized molecules in migrating cells develops asymmetry between two opposite cell poles, the leading edge and the uropod. We show that acquisition of a motile phenotype in T lymphocytes results in the asymmetric redistribution of ganglioside GM3- and GM1-enriched raft domains to the leading edge ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gómez-Móuton C,Abad JL,Mira E,Lacalle RA,Gallardo E,Jiménez-Baranda S,Illa I,Bernad A,Mañes S,Martínez-A C

更新日期：2001-08-14 00:00:00

abstract：:The mature gag and pol proteins of human immunodeficiency virus (HIV) and all retroviruses derive from large gag and gag-pol polyprotein precursors by posttranslational cleavage. A highly specific, virally encoded protease is required for this essential proteolytic processing. In this study, the HIV protease gene prod...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Debouck C,Gorniak JG,Strickler JE,Meek TD,Metcalf BW,Rosenberg M

更新日期：1987-12-01 00:00:00

abstract：:We have obtained long-term cultures of differentiated proliferating follicular cells from normal adult human thyroid glands. In vitro growth of such human cells has been sustained by a modified F-12 medium, supplemented with bovine hypothalamus and pituitary extracts and no added thyrotropin. Cultures have been expand...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Curcio F,Ambesi-Impiombato FS,Perrella G,Coon HG

更新日期：1994-09-13 00:00:00

abstract：:We discuss the pulling force f required to extrude a lipid tube from a living cell as a function of the extrusion velocity L. The main feature is membrane friction on the cytoskeleton. As recently observed for neutrophils, the tether force exhibits a "shear thinning" response over a large range of pulling velocities, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brochard-Wyart F,Borghi N,Cuvelier D,Nassoy P

更新日期：2006-05-16 00:00:00

abstract：:In photosynthesis, light is captured by antenna proteins. These proteins transfer the excitation energy with almost 100% quantum efficiency to the reaction centers, where charge separation takes place. The time scale and pathways of this transfer are controlled by the protein scaffold, which holds the pigments at opti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Müh F,Madjet Mel-A,Adolphs J,Abdurahman A,Rabenstein B,Ishikita H,Knapp EW,Renger T

更新日期：2007-10-23 00:00:00

abstract：:After more than two decades of study, many fundamental questions remain unanswered about the dynamics of glass-forming materials confined to thin films. Experiments and simulations indicate that free interfaces enhance dynamics over length scales larger than molecular sizes, and this effect strengthens at lower temper...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ivancic RJS,Riggleman RA

更新日期：2020-10-13 00:00:00

abstract：:Adjacent paraffin sections of rat hypothalami fixed in Bouin's fluid were treated either with buffer or with luteinizing hormone-releasing hormone (LHRH) before immunocytochemical staining with anti-LHRH. Upon buffer pretreatment, pituitary gonadotrophs were unstained and hypothalamic fibers were stained. Upon LHRH pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sternberger LA,Greenwald JL,Hock D,Elger K,Forssmann WG

更新日期：1981-08-01 00:00:00

abstract：:Despite a growing number of ion channel genes implicated in hereditary ataxia, it remains unclear how ion channel mutations lead to loss-of-function or death of cerebellar neurons. Mutations in the gene KCNMA1, encoding the α-subunit of the BK channel have emerged as responsible for a variety of neurological phenotype...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Du X,Carvalho-de-Souza JL,Wei C,Carrasquel-Ursulaez W,Lorenzo Y,Gonzalez N,Kubota T,Staisch J,Hain T,Petrossian N,Xu M,Latorre R,Bezanilla F,Gomez CM

更新日期：2020-03-17 00:00:00

abstract：:The hypothesis that intracellular Ca2+ is an essential component of the intracellular mechanism of insulin action in the adipocyte was evaluated. Cells were loaded with the Ca2+ chelator quin-2, by preincubating them with quin-2 AM, the tetrakis(acetoxymethyl) ester of quin-2. Quin-2 loading inhibited insulin-stimulat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pershadsingh HA,Shade DL,Delfert DM,McDonald JM

更新日期：1987-02-01 00:00:00

abstract：:Mutants of Arabidopsis that contain reduced levels of polyunsaturated fatty acids showed growth characteristics at 22 degrees C that were very similar to wild type. By contrast, at 12 degrees C, the mutants failed to undergo stem elongation during reproductive growth although they produced normal flowers and fertile s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miquel M,James D Jr,Dooner H,Browse J

更新日期：1993-07-01 00:00:00

abstract：:The "UV footprinting" technique has been used to detect contacts between EcoRI endonuclease and its recognition sequence at single nucleotide resolution. Comparison of the UV-footprinting results to the published crystal structure of the EcoRI endonuclease-DNA complex allows us to determine how UV light detects protei...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Becker MM,Lesser D,Kurpiewski M,Baranger A,Jen-Jacobson L

更新日期：1988-09-01 00:00:00

abstract：:Concern for the suffering of others is central to moral decision making. How humans evaluate others' suffering, relative to their own suffering, is unknown. We investigated this question by inviting subjects to trade off profits for themselves against pain experienced either by themselves or an anonymous other person....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Crockett MJ,Kurth-Nelson Z,Siegel JZ,Dayan P,Dolan RJ

更新日期：2014-12-02 00:00:00

abstract：:Previously, we reported that bilateral excitotoxic lesions of the basolateral nucleus of the amygdala (BLA) block the enhancing effects of posttraining systemic or intrahippocampal glucocorticoid administration on memory for inhibitory avoidance training. The present study further examined the basis of this permissive...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roozendaal B,Nguyen BT,Power AE,McGaugh JL

更新日期：1999-09-28 00:00:00

abstract：:Of two closely related species of geese, one, the greylag goose, lives in the Indian plains all year round, while the other, the bar-headed goose, lives at the Tibetan lakes and migrates across the Himalayas to winter in India. Another species, the Andean goose, lives in the High Andes all year round. Possession of a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jessen TH,Weber RE,Fermi G,Tame J,Braunitzer G

更新日期：1991-08-01 00:00:00

abstract：:The paucity of integral membrane protein structures creates a major bioinformatics gap, whose origin is the difficulty of crystallizing these detergent-solubilized proteins. The problem is particularly formidable for hetero-oligomeric integral membrane proteins, where crystallization is impeded by the heterogeneity an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang H,Kurisu G,Smith JL,Cramer WA

更新日期：2003-04-29 00:00:00

abstract：:Respiratory cytochrome c has been found to be phosphorylated at tyrosine 97 in the postischemic brain upon neuroprotective insulin treatment, but how such posttranslational modification affects mitochondrial metabolism is unclear. Here, we report the structural features and functional behavior of a phosphomimetic cyto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guerra-Castellano A,Díaz-Quintana A,Pérez-Mejías G,Elena-Real CA,González-Arzola K,García-Mauriño SM,De la Rosa MA,Díaz-Moreno I

更新日期：2018-07-31 00:00:00

abstract：:We have analyzed the level of intraindividual sequence variability (heteroplasmy) of mtDNA in human brain by denaturing gradient gel electrophoresis and sequencing. Single base substitutions, as well as insertions or deletions of single bases, were numerous in the noncoding control region (D-loop), and 35-45% of the m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jazin EE,Cavelier L,Eriksson I,Oreland L,Gyllensten U

更新日期：1996-10-29 00:00:00

abstract：:The biodistribution profiles in mice of two pyrrole-imidazole polyamides were determined by PET. Pyrrole-imidazole polyamides are a class of small molecules that can be programmed to bind a broad repertoire of DNA sequences, disrupt transcription factor-DNA interfaces, and modulate gene expression pathways in cell cul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harki DA,Satyamurthy N,Stout DB,Phelps ME,Dervan PB

更新日期：2008-09-02 00:00:00

abstract：:Protein tyrosine phosphorylation and dephosphorylation are key regulatory events in T-cell receptor (TCR) signaling. We investigated the role of the tyrosine phosphatase SHPTP1 in TCR signaling by analysis of TCR signal transduction in motheaten (me/me) mice, which lack SHPTP1 expression. As revealed by flow cytometri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lorenz U,Ravichandran KS,Burakoff SJ,Neel BG

更新日期：1996-09-03 00:00:00

abstract：:Pheromone biosynthesis-activating neuropeptide (PBAN) regulates sex pheromone biosynthesis in female Helicoverpa (Heliothis) zea. Two oligonucleotide probes representing two overlapping amino acid regions of PBAN were used to screen 2.5 x 10(5) recombinant plaques, and a positive recombinant clone was isolated. Sequen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davis MT,Vakharia VN,Henry J,Kempe TG,Raina AK

更新日期：1992-01-01 00:00:00