Abstract:
:Canine ileal segments were luminally perfused with known amounts of polyethylene glycol (PEG), and samples obtained were freed of protein by a cold acetone (CA) method and assayed for PEG by trichloroacetic acid in the presence of calcium cations. This procedure was compared to the Barium salt (BaS) protein precipitation method of Hyden (Ann R Agric Coll Sweden 22:411-424, 1955). The CA method saved specimen volume and time in handling, whereas its stability, linearity, and reproducibility were equivalent to the more complex BaS method. In vitro studies disclosed no appreciable differences when PEG specimens were bile stained or dissolved in various solvents. A small modification of the CA method improved accuracy in measurement of small PEG concentrations.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Buxton TB,Crockett JK,Moore WL 3rd,Moore WL Jr,Rissing JPsubject
Has Abstractpub_date
1979-04-01 00:00:00pages
820-4issue
4eissn
0016-5085issn
1528-0012pii
S001650857900072Xjournal_volume
76pub_type
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