Protein precipitation by acetone for the analysis of polyethylene glycol in intestinal perfusion fluid.

Abstract:

:Canine ileal segments were luminally perfused with known amounts of polyethylene glycol (PEG), and samples obtained were freed of protein by a cold acetone (CA) method and assayed for PEG by trichloroacetic acid in the presence of calcium cations. This procedure was compared to the Barium salt (BaS) protein precipitation method of Hyden (Ann R Agric Coll Sweden 22:411-424, 1955). The CA method saved specimen volume and time in handling, whereas its stability, linearity, and reproducibility were equivalent to the more complex BaS method. In vitro studies disclosed no appreciable differences when PEG specimens were bile stained or dissolved in various solvents. A small modification of the CA method improved accuracy in measurement of small PEG concentrations.

journal_name

Gastroenterology

journal_title

Gastroenterology

authors

Buxton TB,Crockett JK,Moore WL 3rd,Moore WL Jr,Rissing JP

subject

Has Abstract

pub_date

1979-04-01 00:00:00

pages

820-4

issue

4

eissn

0016-5085

issn

1528-0012

pii

S001650857900072X

journal_volume

76

pub_type

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