Abstract:
BACKGROUND & AIMS:Inflammation is created by the movement of leukocytes toward soluble attractants (chemokines). The aim of this study was to identify the chemokines that attract intestinal lymphocytes. METHODS:Intraepithelial and lamina propria lymphocytes were isolated from human jejunal mucosa of healthy individuals and cultured in interleukin (IL) 2 for 3 days. Migration was assessed using the Boyden transwell assay. Increases in cytoplasmic calcium ion concentration ([Ca2+]i) were measured from changes in fluorescence in Fura-2-loaded lymphocytes on exposure to the cytokines. RESULTS:A large number of intraepithelial lymphocytes migrated toward IL-8 and RANTES (regulated on activation, normal T cell expressed and secreted), and a small number migrated toward IL-10. In contrast, only a few lamina propria lymphocytes migrated toward IL-8, and none responded to the other chemokines tested. The chemokines IL-8 and IL-10, but not RANTES, increased [Ca2+]i in intraepithelial lymphocytes; this calcium mobilization was not affected by ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid, indicating that calcium is released from intracellular sources. None of the factors caused calcium mobilization by lamina propria lymphocytes. CONCLUSIONS:This study shows that intraepithelial lymphocytes have greater migratory and calcium-mobilizing activity than lamina propria lymphocytes and that these two activities do not correlate with each other.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Ebert ECdoi
10.1016/0016-5085(95)90573-1subject
Has Abstractpub_date
1995-10-01 00:00:00pages
1154-9issue
4eissn
0016-5085issn
1528-0012pii
0016-5085(95)90573-1journal_volume
109pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract:BACKGROUND & AIMS:Host cell lipid rafts form a scaffold required for replication of hepatitis C virus (HCV). Serine palmitoyltransferases (SPTs) produce sphingolipids, which are essential components of the lipid rafts that associate with HCV nonstructural proteins. Prevention of the de novo synthesis of sphingolipids b...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2013.06.012
更新日期:2013-10-01 00:00:00
abstract::Medical and recreational cannabis use has increased dramatically over the last decade, resulting from mainstream cultural acceptance and legalization in several countries worldwide. Cannabis and its derivatives affect many gastrointestinal processes via the endocannabinoid system (ECS). The ECS influences gastrointest...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1053/j.gastro.2020.03.087
更新日期:2020-07-01 00:00:00
abstract::Isolation of a spiral-shaped bacterium closely related to Helicobacter pylori from the cat stomach made it possible to investigate new small animal models of gastric infection. Pure cultures of this bacterium, provisionally named "Helicobacter felis," were fed to germ-free mice. The organism colonized the stomach in l...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(90)91156-z
更新日期:1990-11-01 00:00:00
abstract:BACKGROUND & AIMS:Krüppel-like factor 5 (KLF5) is a transcription factor that promotes proliferation, is highly expressed in dividing crypt cells of the gastrointestinal epithelium, and is induced by various stress stimuli. We sought to determine the role of KLF5 in colonic inflammation and recovery by studying mice wi...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2010.10.061
更新日期:2011-02-01 00:00:00
abstract::Disordered gustatory acuity was demonstrated in 22 patients with acute viral hepatitis and in 16 patients with chronic liver disease utilizing subjective responses and objective measurements of detection and recognition thresholds and scaling for NaCl, sucrose, HCl, and urea. In patients with early hepatitis and those...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1976-04-01 00:00:00
abstract:BACKGROUND & AIMS:Differences in genetic background may play a role in the development of ulcerative colitis (UC)-related neoplasia. Loss of heterozygosity (LOH) of APC has been reported in human UC-associated neoplasia. To investigate the role of genetic differences in UC-associated neoplasia, we compared differences ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2001.29609
更新日期:2001-12-01 00:00:00
abstract::Incidence of intrahepatic arteriovenous fistula after needle biopsy of the liver, percutaneous transhepatic cholangiography, and transhepatic catheterization of an intrahepatic bile duct or portal vein, was determined by studying the hepatic arteriograms obtained within 1 month of the transhepatic procedure. An arteri...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1978-06-01 00:00:00
abstract::The intestinal epithelium is a repetitive sheet of crypt and villus units with stem cells at the bottom of the crypts. During postnatal development, crypts multiply via fission, generating 2 daughter crypts from 1 parental crypt. In the adult intestine, crypt fission is observed at a low frequency. Using intravital mi...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2017.05.019
更新日期:2017-09-01 00:00:00
abstract::The mortality experienced by a cohort of 653 patients with celiac disease in Edinburgh and the Lothian region has been analyzed. Mortality overall was 1.9-fold (95% confidence limits, 1.5-2.2) that of the general population (115 deaths observed, 61.8 expected; p less than 0.0001). The increased mortality was greatest ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(89)90060-7
更新日期:1989-08-01 00:00:00
abstract::A patient who developed obstructive jaundice as a complication of a liver biopsy is presented. The jaundice was found to be attributable to obstruction of the biliary tree at the level of the bifurcation by an intrahepatic hematoma. The patient had a complete and spontaneous recovery. ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract:BACKGROUND & AIMS:Progressive familial intrahepatic cholestasis can be caused by mutations in ABCB4 or ATP8B1; each encodes a protein that translocates phospholipids, but in opposite directions. ABCB4 flops phosphatidylcholine from the inner to the outer leaflet, where it is extracted by bile salts. ATP8B1, in complex ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2011.07.042
更新日期:2011-11-01 00:00:00
abstract::In vitro neurite outgrowth and neuronal survival are promoted by laminin, and neuronal migration is promoted by collagen type IV. This led to the hypothesis that Hirschsprung's disease (HD) could be the result of an abnormal extracellular matrix microenvironment in the affected bowel during embryogenesis. Using indire...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::In humans and most other species, a decline in pH of gallbladder contents occurs during the concentration of bile. Recent in vitro studies in rabbit, guinea pig, and Necturus gallbladders have strongly suggested mucosal H+ secretion during sodium reabsorption, presumably representing a Na+/H+ exchange. The present in ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(87)90118-1
更新日期:1987-02-01 00:00:00
abstract:BACKGROUND & AIMS:Helicobacter pylori attaches to mucin oligosaccharides that are expressed on host gastric epithelium. We used the rhesus macaque model to characterize the effect of H. pylori infection on gastric mucin oligosaccharides during acute and chronic infection. METHODS:Specific pathogen (H. pylori)-free rhe...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2009.04.014
更新日期:2009-09-01 00:00:00
abstract:BACKGROUND & AIMS:Substance P, a member of the tachykinin family, is a prosecretory neuropeptide distributed widely throughout the enteric nervous system. Implicated in inflammatory states, its role in enterotoxigenic water and electrolyte secretion is unclear. We assessed the effect of substance P antagonists and neur...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2000.18147
更新日期:2000-10-01 00:00:00
abstract::The purpose of these experiments was to determine the contribution of gastrin to the acid secretory response to eating in healthy human subjects. To simulate the gastric and intestinal phases of eating, a meal was homogenized and then infused into the stomach through a nasogastric tube. At the same time, the cephalic ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(87)90968-1
更新日期:1987-04-01 00:00:00
abstract:BACKGROUND & AIMS:Hepatopulmonary syndrome (HPS) affects 10%-30% of patients with cirrhosis and portal hypertension and significantly increases mortality. Studies in experimental models indicate that pulmonary angiogenesis contributes to the development of HPS, but pathogenesis in humans is poorly understood. We invest...
journal_title:Gastroenterology
pub_type: 杂志文章,多中心研究
doi:10.1053/j.gastro.2010.03.044
更新日期:2010-07-01 00:00:00
abstract::Patients with chronic nausea and vomiting frequently present challenging diagnostic and therapeutic problems. In such patients, gastroparesis of unknown cause, or "idiopathic" gastroparesis, may be the only objective finding. Two middle-aged women with nausea, vomiting, and weight loss of 10 and 26 kg over 6 and 18 mo...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(90)91255-5
更新日期:1990-07-01 00:00:00
abstract:BACKGROUND:The prognosis of advanced cirrhosis due to chronic hepatitis B is poor, and results of therapies, including liver transplantation, have been unsatisfactory. Little is known about the effectiveness of interferon alfa in patients with cirrhosis. METHODS:Between 1984 and 1991, 18 patients with clinically-appar...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章
doi:10.1016/0016-5085(93)90281-g
更新日期:1993-04-01 00:00:00
abstract::In a series of in vitro studies, both the soluble (plasmatic) coagulation system and the cellular (platelet-mediated) aspect of coagulation were shown to be extremely sensitive to relatively minor increases in hydrogen ion concentration. All studies became abnormal at pH 6.8. At pH 6.4, assays of the intrinsic and ext...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract:BACKGROUND & AIMS:Protein kinase C (PKC) has been implicated as a mediator of growth control during colorectal carcinogenesis, but the mechanisms involved are still a matter of dispute. The aim of this study was to analyze PKC patterns and tissue distribution to gain further insight in PKC function during tumor develop...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.1996.v110.pm8964400
更新日期:1996-06-01 00:00:00
abstract::Immunologic injury in the liver involves immigrant T and B lymphocytes and a resident lymphoid population that comprises distinct lymphocytic cells and accessory cells. The forerunner to autoimmunity is breaching of natural self-tolerance and hence the disruption of a fundamental property of the immune system. Such br...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1053/gast.2001.22441
更新日期:2001-05-01 00:00:00
abstract:BACKGROUND & AIMS:Cirrhotic liver diseases are associated with abnormalities in cardiac geometry and function in adults (cirrhotic cardiomyopathy) but rarely explored in cirrhotic infants or children. We proposed that features of cirrhotic cardiomyopathy are present in infants with cirrhosis due to biliary atresia (BA)...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2011.06.082
更新日期:2011-10-01 00:00:00
abstract::The acid secretory effect of combined stimulation with balloon distention of the fundus and body of the stomach and intravenous infusion of pentagastrin has been studied in patients with duodenal ulcer (DU) and in healthy subjects. In 8 nonoperated DU subjects and in 5 DU patients subjected to proximal gastric vagotom...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1977-09-01 00:00:00
abstract:BACKGROUND & AIMS:Host genetic factors are likely to contribute to the variable course of hepatic fibrosis in response to chronic liver injury. Similarly, the fibrotic response differs among inbred mouse strains after challenge with CCl(4). Our aim was to identify unknown susceptibility loci for hepatic fibrosis in a c...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2002.37069
更新日期:2002-12-01 00:00:00
abstract::The results of balloon dilatation of upper digestive tract stricture in 111 patients were evaluated. Eighty-eight patients had esophageal strictures and 23 had gastric or pyloric strictures. Thirty-six patients had strictures associated with previous operations. Twenty-two percent of the patients with esophageal stric...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(85)90449-4
更新日期:1985-09-01 00:00:00
abstract::Colonic and intestinal alkaline phosphatase were studied histoenzymatically and biochemically in normal mucosae from eight hemicolectomy specimens. Histochemically, the most intense reaction was found in the small intestine, followed by vascular endothelium and colonic epithelium, respectively. The reaction was strong...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1982-06-01 00:00:00
abstract::The effects of cisapride on jejunal interdigestive motor activity were studied in 12 healthy men participating in three experiments each. Five minutes after an activity front (phase III) they received, in random double-blind fashion, 10 mg of cisapride, 4 mg of cisapride, or saline placebo by intravenous injection. Mo...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0016-5085(86)90387-2
更新日期:1986-05-01 00:00:00
abstract:BACKGROUND & AIMS:MicroRNAs (miRNAs) have been implicated in the development and progression of human cancers. We investigated the roles and mechanisms of miR-26a in human cholangiocarcinoma. METHODS:We used in situ hybridization and quantitative reverse transcriptase polymerase chain reaction to measure expression of...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2012.03.045
更新日期:2012-07-01 00:00:00
abstract:BACKGROUND & AIMS:Simvastatin improves liver generation of nitric oxide and hepatic endothelial dysfunction in patients with cirrhosis, so it could be an effective therapy for portal hypertension. This randomized controlled trial evaluated the effects of continuous simvastatin administration on the hepatic venous press...
journal_title:Gastroenterology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1053/j.gastro.2009.01.043
更新日期:2009-05-01 00:00:00