Abstract:
:The intestinal epithelium is a repetitive sheet of crypt and villus units with stem cells at the bottom of the crypts. During postnatal development, crypts multiply via fission, generating 2 daughter crypts from 1 parental crypt. In the adult intestine, crypt fission is observed at a low frequency. Using intravital microscopy in Lgr5EGFP-Ires-CreERT2 mice, we monitored individual crypt dynamics over multiple days with single-cell resolution. We discovered the existence of crypt fusion, an almost exact reverse phenomenon of crypt fission, in which 2 crypts fuse into 1 daughter crypt. Examining 819 crypts in 4 mice, we found that 3.5% ± 0.6% of all crypts were in the process of fission, whereas 4.1 ± 0.9% of all crypts were undergoing crypt fusion. As counteracting processes, crypt fission and fusion could regulate crypt numbers during the lifetime of a mouse. Identifying the mechanisms that regulate rates of crypt fission and fusion could provide insights into intestinal adaptation to altered environmental conditions and disease pathogenesis.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Bruens L,Ellenbroek SIJ,van Rheenen J,Snippert HJdoi
10.1053/j.gastro.2017.05.019subject
Has Abstractpub_date
2017-09-01 00:00:00pages
674-677.e3issue
3eissn
0016-5085issn
1528-0012pii
S0016-5085(17)35631-7journal_volume
153pub_type
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