Immunoperoxidase assay for anti-dsDNA antibodies using Crithidia luciliae substrates.

Abstract:

:In studies on 779 fresh, unselected ANA+ sera, 50 (6.4%) were found to have anti-dsDNA antibodies, by using CIP (C. luciliae immunoperoxidase), and 84% of the 50 patients met the criteria for definite SLE. After freezing 36 of these sera for 13 +/- 1 months, only 52.8% were again positive by CIP. Using CIF (C. luciliae immunofluorescence), 16.7% and 52.8% of the same stored sera were anti-dsDNA+, with transmitted and epi-illumination, respectively. We favour CIP to CIF because: slide files are permanent, overcoming any lability of antibodies in frozen sera; kinetoplast, flagellum and nucleus are easily distinguished; perikinetoplast staining is avoided; specialized microscopic equipment is not needed.

journal_name

Scand J Rheumatol

authors

Lee TK,Rothberger H

doi

10.3109/03009748509102043

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

386-92

issue

4

eissn

0300-9742

issn

1502-7732

journal_volume

14

pub_type

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