Abstract：
:Using rapid kinetic techniques, we have determined the kinetics of zero-trans influx and equilibrium exchange of adenosine, and its uptake and in situ phosphorylation at 25 degrees C in human erythrocytes which were pretreated with 2'-deoxycoformycin to inhibit deamination of adenosine. Both the Km and Vmax for adenosine transport were about 300 times higher than those for the in situ phosphorylation of adenosine (Km about 0.2 microM), so that the first order rate constants for both processes were about the same. In contrast, the first order rate constant for adenosine deamination by untreated, intact cells was about 20% of that of adenosine transport or phosphorylation. These kinetic properties of the various steps, in combination with substrate inhibition of adenosine phosphorylation above 1 microM adenosine, assure that, at extracellular concentrations of physiological relevance (less than 1 microM), adenosine is very rapidly and efficiently salvaged by the erythrocytes and converted to ATP, whereas at extracellular concentrations of 10 microM or higher, practically all adenosine transported into the cells is deaminated. When the concentration of adenosine was 0.1 microM, a 10% (v/v) suspension of erythrocytes depleted the extracellular fluid of adenosine within 1 min of incubation at 25 degrees C.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Plagemann PG,Wohlhueter RM,Kraupp Mdoi
10.1002/jcp.1041250223subject
Has Abstractpub_date
1985-11-01 00:00:00pages
330-6issue
2eissn
0021-9541issn
1097-4652journal_volume
125pub_type
杂志文章abstract：:An association has been proved between high salt consumption and cardiovascular mortality. In vertebrates, the heart is the first functional organ to be formed. However, it is not clear whether high-salt exposure has an adverse impact on cardiogenesis. Here we report high-salt exposure inhibited basement membrane brea...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26528
更新日期：2018-09-01 00:00:00
abstract：:Mitochondrial (mt) dysfunction and oxidative stress are involved in the pathogenesis of ischemia/reperfusion (I/R)-injury. Lycopene, a lipophilic antioxidant found mainly in tomatoes and in other vegetables and fruits, can protect mtDNA against oxidative damage. However, the role of mtDNA in myocardial I/R-injury is u...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24941
更新日期：2015-09-01 00:00:00
abstract：:Versican is a large chondroitin sulfate proteoglycan and belongs to the family of lecticans. Versican possesses two globular domains, G1 and G3 domain, separated by a CS-attachment region. The CS-attachment region present in the middle region is divided into two spliced domains named CSalpha and beta. Alternative spli...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20921
更新日期：2007-04-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28366
更新日期：2019-08-01 00:00:00
abstract：:Addition of ATP to cultures of transformed mouse fibroblasts, 3T6 cells, resulted in cell growth inhibition, whereas the growth of the non-transformed counterparts, 3T3 cells, was only slightly affected. The inhibition was found to be specific for adenine nucleotides, and concentration dependent. At relatively low con...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041400318
更新日期：1989-09-01 00:00:00
abstract：:Triple-negative breast cancer (TNBC) exhibits a higher level of glycolytic capacity and are commonly associated with an inflammatory microenvironment, but the regulatory mechanism and metabolic crosstalk between the tumor and tumor microenvironment (TME) are largely unresolved. Here, we show that glucose transporter 3...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.30189
更新日期：2021-01-09 00:00:00
abstract：:Using S1 nuclease protection assays, we have examined the representation of cell cycle-dependent H4 histone RNAs in the nuclear matrix and nonmatrix nuclear fractions of human cells. Cytoplasmic and nuclear fractions were prepared from exponentially growing HeLa S3 cells by double detergent (sodium deoxycholate and NP...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041280229
更新日期：1986-08-01 00:00:00
abstract：:The Na+ pump (Na+, K+-ATPase) has been implicated in the regulation of many cellular functions, including cell volume regulation. The effects of inhibiting Na+ pump activity on cell volume and taurine efflux were evaluated in the human neuroblastoma cell line CHP-100. Cell volume changes monitored with the Coulter Mul...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199802)174:2<145::AID-JCP1
更新日期：1998-02-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.10365
更新日期：2003-11-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：2015-08-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041210311
更新日期：1984-12-01 00:00:00
abstract：:Hepatocyte growth factor (HGF) plays a crucial role in the differentiation of skeletal muscle cells, where a process in which the retinoblastoma protein (pRb) has been implicated. We addressed the role of pRb in HGF-mediated effects on the proliferation and differentiation of adult skeletal muscle myoblasts. HGF shift...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.10089
更新日期：2002-05-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041540118
更新日期：1993-01-01 00:00:00
abstract：:This study is performed to examine the impacts of microRNA-218 (miR-218) on cardiac microvascular endothelial cells (CMECs) injury induced by coronary artery disease (CAD). Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) was applied for detecting miR-218 expression in serum of patients with CAD ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29214
更新日期：2020-03-01 00:00:00
abstract：:We have examined the effects of constitutive expression of PTHrP on the growth and differentiation of populations of cells derived from a clonal chondrocytic cell line, CFK2. Cells were stably transfected with cDNA encoding either full-length, secretory PTHrP (CFK2P) or nonsecretory PTHrP (CFK2P-SS). In cultures of ce...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199610)169:1<33::AID-JCP4>
更新日期：1996-10-01 00:00:00
abstract：:The Pur proteins are an ancient family of sequence-specific single-stranded nucleic acid-binding proteins. They bind a G-rich element in either single- or double-stranded nucleic acids and are capable of displacing the complementary C-rich strand. Recently several reports have described Pur family member knockouts, mu...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.24237
更新日期：2013-05-01 00:00:00
abstract：:The C-terminal region of parathyroid hormone-related protein (PTHrP) containing the sequence (107-111) appears to be a potent inhibitor of osteoclastic bone resorption. In the present study, we have investigated the effect of human (h)PTHrP (107-139) and hPTHrP (107-111)NH2 on the proliferation of osteoblastic rat ost...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199702)170:2<209::AID-JCP1
更新日期：1997-02-01 00:00:00
abstract：:Interleukin (IL)-4, which exhibits potent anti-inflammatory activities, is of potential therapeutic value in destructive arthropathies. To further define the response of human joint cells to IL-4, we analyzed the ability of this cytokine to modulate the effects of IL-1beta and growth factors. Freshly isolated chondroc...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.10121
更新日期：2002-07-01 00:00:00
abstract：:Evolving data suggest that marrow hematopoietic stem cells show reversible changes in homing, engraftment, and differentiation phenotype with cell cycle progression. Furthermore, marrow stem cells are a cycling population. Traditional concepts hold that the system is hierarchical, but the information on the lability o...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21273
更新日期：2008-03-01 00:00:00
abstract：:Progesterone is an endogenous immunomodulator and can suppress T-cell activation during pregnancy. We have previously shown that the non-genomic effects of progesterone, especially acidification, are exerted via plasma membrane sites and suppress cellular genomic responses to mitogens. This study aimed to show that ac...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20962
更新日期：2007-05-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20396
更新日期：2005-11-01 00:00:00
abstract：:The regulation of low-density lipoprotein (LDL) receptor activity in the human hepatoma cell line Hep-G2 by serum components was examined. Incubation of dense monolayers of Hep-G2 cells with fresh medium containing 10% fetal calf serum (FM) produced a time-dependent increase in LDL receptor activity. Uptake and degrad...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041350208
更新日期：1988-05-01 00:00:00
abstract：:The prevalence of chronic hyperglycemia and its complications, imposing a critical burden on the worldwide economy and the global healthcare system, is a pressing issue. Mounting evidence indicates that oxidative stress and hypoxia, two noticeable features of hyperglycemia, play a joint crucial role in mediating cellu...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28034
更新日期：2019-08-01 00:00:00
abstract：:Leukotriene C4 is an arachidonic acid metabolite and an important mediator of inflammation and anaphylaxis that is known to induce production of prostacyclin in endothelial cells. The goal of this study was to examine the signal transduction mechanisms activated by leukotriene C4 stimulation. Formation of inositol pho...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199810)177:1<103::AID-JCP1
更新日期：1998-10-01 00:00:00
abstract：:Arg8-vasopressin (AVP) is a potent inducer of myogenic differentiation stimulating the expression of myogenic regulatory factors. To understand the mechanism of its effect on myogenesis, we investigated the early signals induced by AVP in myogenic target cells. In the rat skeletal muscle cell line L6, AVP selectively ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199704)171:1<34::AID-JCP5>
更新日期：1997-04-01 00:00:00
abstract：:An isoform of the Na(+)/Ca(2+) exchanger (SDNCX1.10) was cloned from mesangial cells of Sprague-Dawley rat. Regulation of this isoform was compared to two other clones that were derived from the Dahl/Rapp salt sensitive (SNCX) and salt resistant rat (RNCX). All isoforms differ at the alternative splice site and at ami...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.10447
更新日期：2004-05-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：2015-09-01 00:00:00
abstract：:Scientists of numerous medical and life science disciplines met in Siena, Italy to discuss the latest proceedings in basic and clinical research. General models of interconnected linear and back-feeding cell-cycle control pathways provide a basis for applied molecular research. Cell-cycle determining factors essential...
journal_title：Journal of cellular physiology
pub_type：
doi：10.1002/1097-4652(200012)185:3<481::AID-JCP20>3.0.
更新日期：2000-12-01 00:00:00
abstract：:Restenosis after angioplasty or stent is a major clinical problem. While long noncoding RNAs (lncRNAs) are implicated in a variety of diseases, their role in restenosis is not well understood. This study aims to investigate how dysregulated lncRNAs and messenger RNAs (mRNAs) contribute to restenosis. By microarray ana...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28284
更新日期：2019-02-10 00:00:00
abstract：:Comparative studies with rat transferrin (rTf) and asialotransferrin (asialo-rTf) were performed on suspended adult rat hepatocytes with the aim of elucidating the mechanism of enhanced hepatic Fe acquisition from asialo-rTf observed previously in vivo. At low ligand concentrations (0.05-20 micrograms/ml), the cells b...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041350325
更新日期：1988-06-01 00:00:00
