Antibody to coliform antigens in urine samples from patients with symptoms of urinary tract infection.

Abstract:

:An enzyme linked immunoadsorbent assay (ELISA) was used to detect IgG, IgM, and IgA antibody in urine to a crude antigenic mixture prepared from six arbitrarily chosen strains of common coliform urinary pathogens. In a group of patients with symptoms of urinary tract infection 24 of 80 (30%) urine samples were positive on culture by conventional methods, 58 of 80 (72.5%) were positive for polymorphs on microscopy, and 72 of 80 (90%) were positive for antibody by ELISA. Secretory component was detected in 28 of 80 (35%) samples, and this is consistent with local antibody production. The results show that antibody can be detected in a wide range of urine samples using a simple antigen mixture, and this may form the basis of a useful test to diagnose urinary tract infection.

journal_name

J Clin Pathol

authors

McKenzie H,Young DN

doi

10.1136/jcp.40.7.787

subject

Has Abstract

pub_date

1987-07-01 00:00:00

pages

787-92

issue

7

eissn

0021-9746

issn

1472-4146

journal_volume

40

pub_type

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