Thymidine phosphorylase expression in progression of cervical cancer: correlation with microvessel count, proliferating cell nuclear antigen, and apoptosis.

Abstract:

AIMS:To determine how epithelial and stromal thymidine phosphorylase expression affects angiogenesis, rapid tumour growth, and decreased apoptotic activity in cervical cancer at varying stages of progression. METHODS:Epithelial and stromal thymidine phosphorylase expression, the microvessel count (reflected by factor VIII related antigen), and proliferating cell nuclear antigen (PCNA) were assessed immunohistochemically in 25 specimens of normal cervical epithelium, 35 of carcinoma in situ (CIS), 34 of microinvasive carcinoma, and 34 of invasive cervical squamous cell carcinoma. Apoptosis was evaluated by the terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labelling (TUNEL) method. The relation of epithelial and stromal thymidine phosphorylase expression to microvessel count, PCNA index, and apoptotic index was examined. RESULTS:Epithelial and stromal thymidine phosphorylase expression progressively increased along a continuum from normal epithelium to invasive squamous cell carcinoma. Epithelial and stromal thymidine phosphorylase expression showed a significant positive correlation with microvessel counts. Within each histological stage, CIS cases with high stromal thymidine phosphorylase expression, invasive squamous cell carcinoma cases with high epithelial thymidine phosphorylase expression, and microinvasive carcinoma cases with high thymidine phosphorylase expression in both epithelium and stroma had a significantly higher microvessel count. High epithelial thymidine phosphorylase expression was associated with a significantly higher PCNA index in CIS and microinvasive carcinoma, but not in invasive squamous cell carcinoma. No significant correlation was seen between apoptotic index and either epithelial or stromal thymidine phosphorylase expression or microvessel count. CONCLUSIONS:Epithelial and stromal thymidine phosphorylase expression may combine to promote angiogenesis during progression of cervical cancer, and epithelial thymidine phosphorylase expression may stimulate tumour cell proliferation in the early stages.

journal_name

J Clin Pathol

authors

Fujiwaki R,Hata K,Iida K,Maede Y,Koike M,Miyazaki K

doi

10.1136/jcp.52.8.598

keywords:

subject

Has Abstract

pub_date

1999-08-01 00:00:00

pages

598-603

issue

8

eissn

0021-9746

issn

1472-4146

journal_volume

52

pub_type

杂志文章
  • Microscope slide electrophoresis of serum lipoproteins in agarose gel.

    abstract::An ultramicro electrophoretic technique for the separation of serum lipoproteins is described using agarose gel on microscope slides.The interaction between the agarose gel and lipoproteins has been reduced and the separation between beta- and pre-beta-lipoproteins has been improved over existing methods. A semiquanti...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.24.1.83

    authors: Elphick MC

    更新日期:1971-02-01 00:00:00

  • Adenomatosis of small intestine: case report.

    abstract::A patient complained of severe diarrhoea for about four years. About 2.5 m of the jejunum was resected and duodenal polyps were also removed at laparotomy. Histologically, they were tubular or tubulovillus adenomas, which closely resembled colonic adenomas. No malignant change was detected in these adenomas. Severe in...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.39.9.981

    authors: Nakamura T,Kimura H,Nakano G

    更新日期:1986-09-01 00:00:00

  • A hemalog D analysis of chronic lymphocytic leukaemia and other lymphoproliferative disorders affecting the blood.

    abstract::A correlative clinical and Hemalog D study of 80 cases of chronic lymphocytic leukaemia (CLL) was performed and the results compared with those in other lymphoproliferative disorders affecting the blood (eight cases of hairy-cell leukaemia, HCL; three cases of prolymphocytic leukaemia, PLL: two cases of lymphoplasmacy...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.34.5.473

    authors: Patterson KG,Goldstone AH,Richards JD,Cawley JC

    更新日期:1981-05-01 00:00:00

  • Mucin-producing atypical bronchial carcinoid.

    abstract::In a case of atypical mucin-producing bronchial carcinoid in a 40-year-old man covert metastases were present at the time of lobectomy and were revealed at necropsy--a feature highlighting the malignant potential of the atypical carcinoid. ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.31.12.1218

    authors: Sweeney EC,Cooney T

    更新日期:1978-12-01 00:00:00

  • CYTOENZYMATICAL DIFFERENTIATION OF NORMAL AND NEOPLASTIC PLASMA CELLS.

    abstract::A histochemical method is described of differentiating between normal and neoplastic cells in plasma using the activity of various enzymes characteristic of plasma cells. It was found that in both types of cell increased or diminished activity of cytoenzymes is a useful means of distinguishing between normal and abnor...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.18.3.345

    authors: SZMIGIELSKI S,LITWIN J,ZUPANSKA B

    更新日期:1965-05-01 00:00:00

  • Electrophoretic behaviour of human urinary amylase.

    abstract::A saccharogenic method is described for estimating amylase activity in human urine. Results accord with those reported elsewhere except that in this study the peak in the beta zone is a new finding. Comparison between normal and pathological urines suggests that the amylase activity of the beta peak is not of pancreat...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.18.5.664

    authors: Franzini C

    更新日期:1965-09-01 00:00:00

  • Significance of tests of iron nutrition in pregnancy.

    abstract::Tests of iron and folate nutrition have been carried out in early pregnancy and at delivery in 63 Bantu females. Accepted normal values for serum iron, and percentage saturation of transferrin, but not for unsaturated iron-binding capacity, for non-pregnant subjects were found to apply equally well in pregnancy. Conco...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.19.2.173

    authors: Metz J,Turchetti L,Combrink B,Krawitz S

    更新日期:1966-03-01 00:00:00

  • Surface marker and other characteristics of Gaucher's cells.

    abstract::A full surface marker study of the splenic storage cells in a case of Gaucher's disease largely substantiates the monocyte/histiocyte nature of Gaucher's cells. In addition, an apparent T-lymphocyte deficiency is demonstrated in the spleen and peripheral blood, and the possible significance of this finding is discusse...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.30.10.981

    authors: Burns GF,Cawley JC,Flemans RJ,Higgy KE,Worman CP,Barker CR,Roberts BE,Hayhoe FG

    更新日期:1977-10-01 00:00:00

  • THE PLANNING OF BENCHING AND SERVICES FOR THE MODERN PATHOLOGICAL LABORATORY.

    abstract::The principles which should govern the planning of benching and services for new laboratories are enumerated. These include the necessity for long-term planning, flexibility, and economy. The ;Cardiff benching system' where the benches are composed of units that consist of an independent movable table base, a removabl...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.16.5.480

    authors: LAURENCE KM

    更新日期:1963-09-01 00:00:00

  • Inflammatory bowel disease biopsies: updated British Society of Gastroenterology reporting guidelines.

    abstract::Accurate histopathological assessment of biopsies is important for the diagnosis, subclassification, and management of chronic idiopathic inflammatory bowel disease (IBD). British Society of Gastroenterology (BSG) guidelines for the initial histopathological diagnosis of IBD were published in 1997. Changes since then ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章,实务指引

    doi:10.1136/jclinpath-2013-201885

    authors: Feakins RM,British Society of Gastroenterology.

    更新日期:2013-12-01 00:00:00

  • The dispersal of staphylococci in hospital wards.

    abstract::The air of three hospital wards was examined frequently for staphylococci for a period of nearly four years using slit samplers. ;Broadcasts' of staphylococci into the air were observed with the air count rising well above the mean of 0.2 staphylococcal particle per cubic foot and these broadcasts often appeared to be...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.15.6.552

    authors: Noble WC

    更新日期:1962-11-01 00:00:00

  • Growth hormone and malignancy.

    abstract::The finding of raised growth hormone concentrations in patients with secondary malignancy in the liver is reported. These concentrations were significantly higher than those found in patients with primary malignancy only or in those with non-hepatic secondaries. A wide range of malignancies was investigated and the hi...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.36.8.935

    authors: Andrews GS

    更新日期:1983-08-01 00:00:00

  • Primary squamous cell carcinoma of the terminal ileum.

    abstract::A case of squamous cell carcinoma of the terminal ileum with no underlying duplication or inflammatory disorder is described. The neoplasm seemed to have originated from the surface epithelium, invading the wall and metastasising to the regional lymph nodes. The 65 year old patient was free of disease three years afte...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.44.3.253

    authors: Platt CC,Haboubi NY,Schofield PF

    更新日期:1991-03-01 00:00:00

  • Epstein Barr virus (EBV) encoded small RNAs: targets for detection by in situ hybridisation with oligonucleotide probes.

    abstract:AIMS:To develop a rapid, sensitive, and specific non-isotopic in situ hybridisation (NISH) procedure for the detection of Epstein-Barr virus in formalin fixed, paraffin wax embedded tissues. METHODS:Two low molecular weight RNAs, designated EBER-1 and EBER-2 (Epstein-Barr encoded RNA), were used: cells latently infect...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.45.7.616

    authors: Khan G,Coates PJ,Kangro HO,Slavin G

    更新日期:1992-07-01 00:00:00

  • Mucosal argyrophil endocrine cells in pernicious anaemia and upper gastrointestinal carcinoid tumours.

    abstract::The number and density of argyrophil endocrine cells were morphometrically calculated in gastric fundal mucosal biopsy specimens taken from 64 patients with pernicious anaemia (five with gastric carcinoids, 15 with nodular argyrophil cell hyperplasia, 44 with diffuse argyrophil cell hyperplasia) and from 14 healthy co...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.42.4.371

    authors: Sjöblom SM,Sipponen P,Karonen SL,Järvinen HJ

    更新日期:1989-04-01 00:00:00

  • Squamous differentiation in carcinoma in situ of the cervix uteri. A cyto-histological correlation of malignant intraepithelial lesions with invasive carcinoma.

    abstract::Cervical biopsies from 97 women with malignant cells in cervical scrape smears have been studied. Forty-eight patients had invasive squamous carcinoma, and 49 had intraepithelial lesions. Of these, six had dedifferentiated carcinoma in situ, nine had the differentiated lesions generally known as ;severe dysplasia', an...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.20.1.7

    authors: Grubb C,Janota I

    更新日期:1967-01-01 00:00:00

  • Cultural method for large-scale screening for Chlamydia trachomatis genital infection.

    abstract::Idoxuridine-treated McCoy cells grown as monolayers in 96 well microplates provide a convenient method for the isolation of Chlamydia trachomatis. Staining of infected monolayers with periodic acid-Schiff reagent (PAS) allows easy recognition of C trachomatis inclusions without the need for dark-ground microscopy. By ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.34.7.712

    authors: Mallinson H,Sikotra S,Arya OP

    更新日期:1981-07-01 00:00:00

  • Coagulase-negative staphylococci causing endocarditis after cardiac surgery.

    abstract::The laboratory findings in seven cases of endocarditis caused by coagulase-negative staphylococci are presented. The usefulness of biochemical characterization of the strains in diagnosis and the selection of antibiotics for prophylaxis and treatment are discussed. ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.26.7.517

    authors: Speller DC,Mitchell RG

    更新日期:1973-07-01 00:00:00

  • Meningitis due to Gemella haemolysans after radiofrequency trigeminal rhizotomy.

    abstract::Meningitis due to Gemella haemolysans developed in a 73 year old woman after thermolysis of the Gasserian ganglion for trigeminal neuralgia. The taxonomy of this organism is discussed, and previous cases of infection are reviewed. ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.38.5.558

    authors: Mitchell RG,Teddy PJ

    更新日期:1985-05-01 00:00:00

  • Sea blue histiocytosis: a common abnormality of the bone marrow in myelodysplastic syndromes.

    abstract:AIMS:To determine whether myelodysplastic syndromes (MDS) are associated with sea blue histiocytosis in the bone marrow. METHODS:A retrospective review of bone marrow aspirates from 35 patients presenting consecutively with MDS and from 20 patients with each of the following: normal marrow appearance (routine staging ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.46.11.1030

    authors: Howard MR,Kesteven PJ

    更新日期:1993-11-01 00:00:00

  • Cerebral infection with Escherichia coli O157:H7 in humans and gnotobiotic piglets.

    abstract::Escherichia coli O157:H7 was isolated from a fatal case of haemorrhagic colitis with haemolytic uraemic syndrome and neurological symptoms. This strain induced diarrhoea and neurological symptoms including incoordination, ataxia, and convulsions in piglets after oral inoculation. Similar neurological signs were seen i...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.41.10.1099

    authors: Tzipori S,Chow CW,Powell HR

    更新日期:1988-10-01 00:00:00

  • Nuclear beta-catenin and Ki-67 expression in choriocarcinoma and its pre-malignant form.

    abstract:OBJECTIVE:To study the expression of nuclear beta-catenin and Ki-67 in patients with normal gestation products (NGP), complete hydatidiform moles (CHM), and choriocarcinoma to elucidate their roles in carcinogenesis and their interrelations. METHODS:Expression of nuclear beta-catenin and Ki-67 was studied by immunohis...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2005.026666

    authors: Wong SC,Chan AT,Chan JK,Lo YM

    更新日期:2006-04-01 00:00:00

  • Fluorimetric assay of mycobacterial group-specific hydrolase enzymes.

    abstract::Fluorigenic substrates prepared from 4-methylumbelliferone provide a simple, convenient method for detecting and assaying group-specific hydrolase activity in small quantities of whole mycobacteria. Physico-chemical properties of the enzymes such as pH dependence and heat stability may also be studied. A technique is ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.31.4.378

    authors: Grange JM

    更新日期:1978-04-01 00:00:00

  • The value of a study of the mucosubstances in rectal biopsies from patients with carcinoma of the rectum and lower sigmoid in the diagnosis of premalignant mucosa.

    abstract::One hundred and twenty-one rectal biopsies from 99 patients with carcinoma of the rectum or lower sigmoid colon were investigated using a high iron-diamine-Alcian blue technique for sulphated and non-sulphated acid mucins. It was found that in normal rectal mucosa sulphomucins are the main carbohydrate component of th...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.25.2.123

    authors: Filipe MI

    更新日期:1972-02-01 00:00:00

  • A rapid method for staining inclusions of Chlamydia psittaci and Chlamydia trachomatis.

    abstract::A new staining method was developed for the detection of inclusions of Chlamydia psittaci and Chlamydia trachomatis inclusions in cell cultures. Using a combination of methyl green and neutral red stains and washing at pH 5.0, inclusions were stained red while cell cytoplasm was pale pink and cell nuclei were pale gre...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.35.6.642

    authors: Woodland RM,Malam J,Darougar S

    更新日期:1982-06-01 00:00:00

  • Microbiological investigation of polyarthralgia.

    abstract::Results of serological investigations on patients with joint pain, arthralgia or polyarthritis were analysed and this information was used to develop a diagnostic algorithm to ensure optimal utilisation of laboratory resources. Accordingly, all cases are now examined for parvovirus IgM, mycoplasma IgM and streptococca...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.48.8.777

    authors: Jones JW,Pether JV,Frost RW

    更新日期:1995-08-01 00:00:00

  • Radioimmunoassay of serum glycocholic acid, standard laboratory tests of liver function and liver biopsy findings: comparative study of children with liver disease.

    abstract::Serum glycocholic acid (SGC) was measured by radioimmunoassay in 277 samples from 122 children with hepatobiliary disorders and from 23 healthy age-matched controls. In patients with hepatobiliary disease the SGC was more frequently abnormal (83%) than values for serum albumin (7%), prothrombin time (17%), bilirubin (...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.35.9.1011

    authors: Matsui A,Psacharopoulos HT,Mowat AP,Portmann B,Murphy GM

    更新日期:1982-09-01 00:00:00

  • Rapid diagnosis of respiratory syncytial virus infection in children by the immunofluorescent technique.

    abstract::The immunofluorescent examination of nasopharyngeal secretions has been compared with conventional cell culture for the diagnosis of respiratory syncytial (RS) virus infection in children under the age of 2 years in Newcastle upon Tyne and Manchester. Two hundred and sixty-eight identifications of RS virus were made b...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.24.4.308

    authors: Cradock-Watson JE,McQuillin J,Gardner PS

    更新日期:1971-05-01 00:00:00

  • Erythrocyte radiolabelling: in vitro comparison of chromium, technetium, and indium in undamaged and heat damaged cells.

    abstract::The stability of the commonly used red cell radio labels chromium-51, indium-(111 or 113m), and technetium-99m, within intact red cells and stroma and their distribution within the cell were compared in undamaged and heat damaged red cells in relation to the clinical use of heat damaged cells in the assessment of sple...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.39.7.717

    authors: Peters AM,Osman S,Reavy HJ,Chambers B,Deenmamode M,Lewis SM

    更新日期:1986-07-01 00:00:00

  • An assessment of techniques suitable for the diagnosis of sickle-cell disease and haemoglobin C disease in cord blood samples.

    abstract::Agar gel, cellulose acetate, and starch gel electrophoresis are all capable of diagnosing sickle-cell anaemia, sickle-cell haemoglobin C disease, and haemoglobin C disease in cord blood samples. Of these three electrophoretic techniques, agar gel is the easiest to interpret.Paper electrophoresis can reliably and rapid...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.23.6.533

    authors: Yaswon GI,Huntsman RG,Metters JS

    更新日期:1970-09-01 00:00:00