Fluorimetric assay of mycobacterial group-specific hydrolase enzymes.

Abstract:

:Fluorigenic substrates prepared from 4-methylumbelliferone provide a simple, convenient method for detecting and assaying group-specific hydrolase activity in small quantities of whole mycobacteria. Physico-chemical properties of the enzymes such as pH dependence and heat stability may also be studied. A technique is described for studying glycosidase, hexosaminidase, acid phosphatase, and acyl esterase activity in seven mycobacterial species. Such techniques will be a useful aid to the taxonomy, identification, and quantification of mycobacteria, especially slowly growing strains in which biochemical properties are very difficult to detect by other techniques.

journal_name

J Clin Pathol

authors

Grange JM

doi

10.1136/jcp.31.4.378

subject

Has Abstract

pub_date

1978-04-01 00:00:00

pages

378-81

issue

4

eissn

0021-9746

issn

1472-4146

journal_volume

31

pub_type

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