Abstract:
AIMS:To estimate the expression of substance P in the haematopoietic cells of hypoplastic bone marrow and define its relationship with the course of bone marrow hypoplasia. METHODS:Bone marrow specimens were obtained from 42 children with bone marrow hypoplasia who were hospitalised in the Department of Pediatric Oncology, Hematology and Transplantology, Poznan University of Medical Sciences, Poznan, Poland, between 1996 and 2003. Substance P and Ki-67 expression were evaluated using immunochemical and hybridocytochemical assays. RESULTS:The expression of substance P (as evidenced by both immunocytochemical and hybridisation techniques) was confirmed in the cytoplasm of B lymphocytes in 8 of 11 children who developed acute leukaemia in 45 (SD 12) days. The percentage of substance P-positive cells ranged from 67.6 to 95.8 (mean of 81.5% cells with immunocytochemistry and 84.3% with in situ hybridisation). The risk of development of leukaemia secondary to bone marrow hypoplasia was found to be significant (p<0.001) in those children who expressed substance P in normal-looking lymphocytes at the initial bone marrow evaluation. CONCLUSIONS:The presence of substance P in B lymphocytes of hypoplastic bone marrow may predict its neoplastic transformation. A marked correlation between substance P-positive bone marrow pattern and the expansion of tumour cells may prove the potential value of this oligopeptide in the pathogenesis of leukaemia.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Nowicki M,Ostalska-Nowicka D,Konwerska A,Miskowiak Bdoi
10.1136/jcp.2005.034629subject
Has Abstractpub_date
2006-09-01 00:00:00pages
935-41issue
9eissn
0021-9746issn
1472-4146pii
59/9/935journal_volume
59pub_type
杂志文章abstract::Several diseases of the small intestine, including gluten-sensitivity, present with malabsorption and a "flat" mucosa. Determination of the mitotic index of epithelial lymphocytes provides a simple, objective method of assessing, and thus of predicting, whether a flat mucosa is due to gluten-sensitivity (index greater...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.2.149
更新日期:1983-02-01 00:00:00
abstract::Patients with congenital lung cysts are at increased risk of developing carcinoma, but the mechanisms concerned are not clear. The case of a young adult who developed a bronchioloalveolar carcinoma associated with a cystic congenital adenomatoid malformation is reported. The adjacent lung showed an unusual intra-alveo...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.6.612
更新日期:1987-06-01 00:00:00
abstract:AIM:To assess the reliability of local international normalised ratios (INR) using a set of three international reference preparation (IRP) certified freeze dried plasmas. METHODS:55 centres in the United Kingdom and the Republic of Ireland participated; 36 centres employed coagulometers and 19 a manual prothrombin ti...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1136/jcp.51.5.356
更新日期:1998-05-01 00:00:00
abstract:OBJECTIVE:Despite advances in therapy, lung cancer is still the leading cause of cancer-related mortality in the world. Further prognostic tools are warranted for risk-adapted therapeutic decisions. We analysed a cohort of primary surgically treated non-small cell lung cancers (NSCLCs) to determine the prognostic role ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202819
更新日期:2015-05-01 00:00:00
abstract:AIM:To determine the frequency of skip lesions in an unselected series of temporal artery biopsies and compare the results with other series. METHODS:The study was a retrospective review of 102 consecutive temporal artery biopsies taken in a five year period (1992-1997) in one large hospital. RESULTS:35 cases (34.3%)...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.53.2.137
更新日期:2000-02-01 00:00:00
abstract::Immune and non-immune rubella sera were examined in the haemagglutination inhibition (HAI) test to evaluate the removal of lipoprotein non-specific inhibitors (NSI) of haemagglutination after kaolin and manganous chloride/heparin treatments. The sera were titrated fresh or after lipoprotein deterioration brought about...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.30.5.409
更新日期:1977-05-01 00:00:00
abstract:AIM:To develop a simple but reliable polymerase chain reaction (PCR) method to determine the HIV-1 status of patients on formalin fixed, paraffin wax embedded lymph node tissue. METHODS:Fifty lymph node specimens, 20 from HIV-1 seropositive and 30 from HIV-1 seronegative patients, were analysed. Lymph nodes with a var...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.8.733
更新日期:1995-08-01 00:00:00
abstract:AIMS:To determine important factors influencing recurrence after local excision of duct carcinoma in situ (DCIS) of the breast. MATERIALS AND METHODS:The extent (size) in millimetres, classification (by cytonuclear grade (NHSBSP system), by extent of necrosis, and by the Van Nuys system), and excision margins of 115 c...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.55.8.581
更新日期:2002-08-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.30.7.653
更新日期:1977-07-01 00:00:00
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journal_title:Journal of clinical pathology
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更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Microscopic examination of scalp hair can provide important diagnostic information in a range of paediatric conditions. It is a non-invasive and cost effective investigation, which is not widely performed. AIMS:To examine retrospectively the value of hair examination by light microscopy, including polarisin...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.027581
更新日期:2005-12-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.51.10.765
更新日期:1998-10-01 00:00:00
abstract:AIM:The earlier detection of breast cancer through mammographic screening has resulted in a shift in stage distribution with patients who are node-positive tending to present with a lower number of positive lymph nodes (LN). This study aims to assess the prognostic value of absolute number of positive nodes in the pN1 ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2012-200755
更新日期:2012-07-01 00:00:00
abstract::Attempts to detect hepatitis B antigen in faeces and bile should take into account the degradation and disappearance of the surface antigens in an environment containing proteolytic enzymes in the presence of bile salts as in the intestinal lumen. The cores of the Dane particles are much more stable than the surface a...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.27.9.693
更新日期:1974-09-01 00:00:00
abstract::A 19 year old man presented with unilateral testicular swelling and pain. An initial diagnosis of epididymo-orchitis was modified to a presumed testicular neoplasm following ultrasonography. The final diagnosis of isolated testicular vasculitis was established following histological examination of the orchidectomy spe...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.12.1121
更新日期:1994-12-01 00:00:00
abstract::Pseudomonas cepacia (Ps. multivorans) can become adapted to a 1 in 30 dilution of Savlon hospital concentrate in distilled water, and can multiply in it. The organism so adapted did not survive in Savlon of the same strength made with hard tap water (pH 7.2). This effect was due to the difference of pH between the two...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.24.8.708
更新日期:1971-11-01 00:00:00
abstract::The API 20 STREP system was used to identify 146 known strains from seven species of the genus Listeria, including both pathogenic and environmental strains. The gallery was easy to use and tests, with the exception of leucine arylamidase (LAP) and starch fermentation (AMD), were simple to interpret. Identification to...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.5.548
更新日期:1989-05-01 00:00:00
abstract::Sera can produce nuclear or perinuclear immunofluorescence staining in neutrophils which may be caused by antibodies with differing antigenic specificities. These include perinuclear antineutrophil cytoplasmic antibodies (P-ANCA), granulocyte specific antinuclear antibody (GS-ANA), and antinuclear antibody (ANA). Ther...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.52.6.475
更新日期:1999-06-01 00:00:00
abstract::In a twenty-year period 19 appendicectomy specimens were diagnosed as primary Crohn's disease. This represents 4.9% of the total number of both resection specimens and mucosal biopsies diagnosed as Crohn's disease during that time. This is a review of the main histopathological features found in these appendices and t...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.6.632
更新日期:1983-06-01 00:00:00
abstract:BACKGROUND:In primary aldosteronism (PA) the main source of aldosterone hypersecretion is an aldosterone-producing adenoma (APA) or a bilateral hyperplasia. Histopathology of the adrenal gland from patients with PA has been difficult, as there are no morphological criteria to ascertain which are the cells that produce ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2012-201287
更新日期:2013-04-01 00:00:00
abstract:AIMS:Glypican 3 (GPC3) is a heparan sulphate proteoglycan that shows elevated levels in the serum of patients with hepatocellular carcinoma (HCC), but not in healthy blood donors or patients with benign liver disease. This study explores the value of GPC3 expression for diagnosis of HCC by immunohistochemistry in liver...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2010.075374
更新日期:2010-07-01 00:00:00
abstract:BACKGROUND:Well differentiated liposarcomas (WDLPS) and dedifferentiated liposarcomas (DDLPS) have been shown to have supernumerary chromosomes with amplified sequences of the MDM2 and CDK4 genes. MDM2 and CDK4 protein overexpression have also been identified in these tumours. AIM:To investigate whether immunohistoche...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2009.070201
更新日期:2009-12-01 00:00:00
abstract::The morphological classification and diagnosis of bladder neoplasms is summarised, with specific focus on histological typing, grading and staging. Four diagnostic categories are described on the basis of the pattern of growth of the urothelial lesions and tumours (flat, exophytic or papillary, endophytic, and invasiv...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2007.049312
更新日期:2008-01-01 00:00:00
abstract::The effect of acute hypoglycaemia on platelet function was examined in patients undergoing an insulin stress test. Enhanced platelet aggregation was observed in all cases but platelet count, platelet adenine nucleotides, and the plasma level of von Willebrand factor were unchanged overall. The onset of the hypoglycaem...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.5.434
更新日期:1979-05-01 00:00:00
abstract::Five hundred and seven strains of bacteria isolated from the urine of patients with significant bacteriuria (more than 10(8) colony-forming units per litre) were tested for sensitivity to co-trimoxazole by the agar diffusion technique. Each organism was tested with a combined disk containing trimethoprim and sulphamet...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.2.199
更新日期:1981-02-01 00:00:00
abstract::Of 30 children with bleeding disorders exposed to an index tuberculosis case, six developed primary pulmonary tuberculosis and two others became positive skin reactors. There was a significant correlation between the amount of replacement treatment received and the development of evidence of tuberculous infection. Onl...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.10.1163
更新日期:1985-10-01 00:00:00
abstract::Thirty-seven percutaneous renal biopsies showing no significant abnormalities on light microscopy were studied electron optically and by immunofluorescence when available. Assessment of the pathological material was followed by analysis of the patients' clinical notes, and a clinicopathological correlation was carried...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.29.5.380
更新日期:1976-05-01 00:00:00
abstract:PURPOSE:To investigate p53 and hepatoma-derived growth factor (HDGF) expression and their association with clinicopathological features of Ewing family tumour (EFT). EXPERIMENTAL DESIGN:A total of 108 cases of EFT were retrospectively analysed. p53 and HDGF expression were detected using immunohistochemistry, and the ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2013-201705
更新日期:2014-03-01 00:00:00
abstract::Evaluation of histological diagnosis requires an index of agreement (to measure repeatability and validity) together with a method of assessing bias. Cohen's kappa statistic appears to be the most suitable tool for measuring levels of agreement, which if unsatisfactory may be caused by bias. Further study of bias is p...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.11.1269
更新日期:1983-11-01 00:00:00
abstract::A biochemical typing method is described for Klebsiella pneumoniae (sensu lato) and Enterobacter aerogenes. The technique depends on differences in metabolism of five carbon substrates--glycerol, inositol, lactose, glucose, and xylose--at two concentrations. Reproducibility is satisfactory and is monitored by the inco...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.9.935
更新日期:1979-09-01 00:00:00