Abstract:
AIM:To develop a simple but reliable polymerase chain reaction (PCR) method to determine the HIV-1 status of patients on formalin fixed, paraffin wax embedded lymph node tissue. METHODS:Fifty lymph node specimens, 20 from HIV-1 seropositive and 30 from HIV-1 seronegative patients, were analysed. Lymph nodes with a variety of disease conditions were included in the study. Tissue sections were treated with a DNA extraction buffer containing proteinase K and the crude cell lysate was used in PCR analysis. Nested primers were used to amplify HIV-1 DNA sequences coding for gag, pol and env proteins. PCR products were demonstrated by polyacrylamide gel electrophoresis. Results were then compared with HIV-1 serology of the patients from whom the tissue was obtained. RESULTS:The PCR method yielded a specificity of 100%, a sensitivity of 95%, a positive predictive value of 100%, and a negative predictive value of 97% when compared with HIV-1 serology. The kappa statistic (0.958) showed an excellent agreement between the PCR method and serology. Furthermore, HIV-1 DNA was demonstrated in lymph node tissue from a serologically unconfirmed acquired immunodeficiency syndrome case necropsied in 1982. CONCLUSION:This PCR method is a simple and reliable means of retrospectively determining the HIV-1 status of patients using formalin fixed, paraffin wax embedded lymph node tissue.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Slavik T,Wolfaardt M,van Zyl H,Simson IWdoi
10.1136/jcp.48.8.733subject
Has Abstractpub_date
1995-08-01 00:00:00pages
733-6issue
8eissn
0021-9746issn
1472-4146journal_volume
48pub_type
杂志文章abstract::Of the 123 patients with acute mesenteric infarction treated over the past 12 years, 16 (13%) had mesenteric venous thrombosis. Eight of the patients with mesenteric venous thrombosis survived the initial episode; two have since died. The remaining six patients were studied for evidence of haemostatic deficiencies or ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.8.906
更新日期:1987-08-01 00:00:00
abstract::Enteroviruses are rare causes of acute focal encephalitis. A fatal case of echovirus type 9 infection is reported in a 9 month old boy who presented with a fever and a macular rash followed by two focal seizures. Echovirus type 9 was isolated from lung tissue after seven days. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.9.865
更新日期:1993-09-01 00:00:00
abstract::A case of squamous cell carcinoma of the terminal ileum with no underlying duplication or inflammatory disorder is described. The neoplasm seemed to have originated from the surface epithelium, invading the wall and metastasising to the regional lymph nodes. The 65 year old patient was free of disease three years afte...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.44.3.253
更新日期:1991-03-01 00:00:00
abstract::Sperm-agglutinating antibodies were present in three (2-6%) of 117 fertile men and in 19 (33-9%) of 56 men who had been vasectomized. Twenty-four of the 56 vasectomized men had been studied before vasectomy; sperm-agglutinating antibodies were present in one (4-3%) compared with eight (33-3%) after vasectomy. No sperm...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.29.12.1127
更新日期:1976-12-01 00:00:00
abstract:AIM:Thalassaemia is a good candidate disease for control by preventive genetic programmes in developing countries. Accurate population frequency data are needed for planning the control of thalassaemia in the high risk Guangdong Province of southern China. METHODS:In total, 13397 consecutive samples from five geograph...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2003.014456
更新日期:2004-05-01 00:00:00
abstract:AIMS:To investigate the expression of the placental cell-specific associated proteins in infantile haemangioma (IH). METHODS:Immunohistochemical staining was used to investigate the expression of human chorionic gonadotrophin (hCG), human placental lactogen (hPL), human leucocyte antigen-G (HLA-G), cytokeratin 7 (CK7)...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2011-200191
更新日期:2011-10-01 00:00:00
abstract::Factor VIII-containing materials were administered to four severely affected haemophiliacs twice weekly in doses calculated to raise the factor VIII level to either 15% or 30% of average normal. The pooled results from those patients with statistically similar baseline bleeding frequencies showed a significant reducti...
journal_title:Journal of clinical pathology
pub_type: 临床试验,杂志文章
doi:10.1136/jcp.30.1.65
更新日期:1977-01-01 00:00:00
abstract::Factor VIII procoagulant activity (VIII:C), factor VIII related antigen (VIIIR:AG), and the von Willebrand factor (VIII:WF) were measured in 19 patients with liver disease (8 cases of alcoholic cirrhosis and 11 with acute viral hepatitis). In both groups of patients the levels of VIII:C, VIIIR:AG, and VIIIR:WF were ab...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.30.3.221
更新日期:1977-03-01 00:00:00
abstract::A 5 month old girl had typical clinical features of acute myocarditis just after the febrile period of exanthem subitum and died immediately. She had been healthy, with normal development, and there was no family history of particular note. Myocardial postmortem findings were compatible with acute myocarditis. Althoug...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.54.10.792
更新日期:2001-10-01 00:00:00
abstract::Conventional prostate adenocarcinomas consist mainly of tumour cells of luminal immunophenotype with scattered neuroendocrine (NE) cells. NE cells are defined by chromogranin A (CGA) immunoreactivity. Unlike luminal cells, NE cells lack androgen receptor (AR) and prostate specific antigen (PSA) immunoreactivity. This ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2003.010207
更新日期:2004-03-01 00:00:00
abstract::A patient with a 10 year history of monoclonal gammopathy of undetermined significance and Bence Jones proteinuria, and a 44 year history of ankylosing spondylitis, developed a nephrotic syndrome secondary to renal amyloidosis. Clinically the amyloidosis was ascribed to Bence Jones proteinuria rather than to the burnt...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.45.10.934
更新日期:1992-10-01 00:00:00
abstract::Human sperm heads were detected and tracked using semiautomatic image analysis. Measurements of size and shape on two specimens from each of 26 men showed that the major component of variability both within and between subjects was the number of small elongated sperm heads. Variability of the computed features between...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.39.12.1347
更新日期:1986-12-01 00:00:00
abstract:AIMS:To determine the clinical performance of three cerebrospinal fluid (CSF) IgG synthesis formulae using data obtained from two quantitation methods. METHODS:Receiver operator characteristic (ROC) analysis and decision index plots were used to compare a rate nephelometric (RN) and a rocket immunoelectrophoretic (RIE...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.49.1.24
更新日期:1996-01-01 00:00:00
abstract:AIM:To compare the sensitivity and staining pattern of the new immunohistochemical antibody to tyrosinase (T311) with S-100, HMB45, and the recently evaluated antibody to melan-A (A103) in a range of melanocytic lesions. METHOD:Archival, formalin fixed, paraffin wax embedded sections from 50 benign and malignant melan...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.54.3.196
更新日期:2001-03-01 00:00:00
abstract::Bilateral adrenalectomy produces a reduction in the alkaline phosphatase concentration in rat intestine, which is not prevented by the administration of saline, but is prevented by the administration of cortisone. The administration of A.C.T.H. to normal rats leads to a rise in intestinal alkaline phosphatase levels a...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.20.2.185
更新日期:1967-03-01 00:00:00
abstract:AIM:To establish the diagnostic value of in situ hybridisation and the nested polymerase chain reaction (PCR) in detecting clinically relevant cytomegalovirus (CMV) infection in upper gastrointestinal biopsies from heart transplant patients. METHODS:Test sensitivity and specificity for detection of CMV early gene RNA ...
journal_title:Journal of clinical pathology
pub_type: 临床试验,杂志文章
doi:10.1136/jcp.51.11.807
更新日期:1998-11-01 00:00:00
abstract:AIMS:(1) To investigate the immunohistochemical expression of the multidrug resistance gene (MDR1) product P-glycoprotein in histological samples from 31 hepatocellular carcinomas (HCCs); and (2) to correlate the results with cell proliferation, p53 expression, the disease-free interval, and cumulative patient survival...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.49.6.470
更新日期:1996-06-01 00:00:00
abstract::In the course of the Paris study on risk factors of cardiovascular disease in a large professional group, 7710 active and apparently healthy men aged between 48 and 54 were examined. This study measured the relationship between clinical abnormalities suggesting alcoholic liver disease (ALD) and the following blood par...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.7.654
更新日期:1978-07-01 00:00:00
abstract::A rapid and reproducible technique for in situ hybridisation, using biotin labelled probes for the Y chromosome, human DNA, hepatitis B virus DNA and cytomegalovirus DNA on formalin fixed, paraffin embedded liver tissue, was developed. The degree of proteolytic digestion of tissue specimens is critical to ensure adequ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.7.793
更新日期:1988-07-01 00:00:00
abstract:AIMS:To compare the efficacy of and tolerance to oral fluconazole and intraconazole in preventing fungal infection in neutropenic patients with haematological malignancies. PATIENTS:213 consecutive, afebrile adult patients treated with or without autologous stem cell transplantation for haematological malignancies. M...
journal_title:Journal of clinical pathology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1136/jcp.52.5.376
更新日期:1999-05-01 00:00:00
abstract:AIM:Heart failure (HF) affects millions of older individuals in both developed and low/middle-income countries. Serum galectin-3 levels have been shown to have prognostic value. However, its use as a diagnostic biomarker has not been explored. The aim was to establish a saliva galectin-3 reference range and to demonstr...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2016-203631
更新日期:2016-12-01 00:00:00
abstract::A panel of three monoclonal antibodies were used in an immunoalkaline phosphatase staining method on a series of serous effusion samples from cases of mesothelioma, lung carcinoma, and benign disease. The antibodies used were anti-carcinoembryonic (CEA) antigen, Ca, and anti-human milk fat globule membrane antigen. An...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.12.1424
更新日期:1987-12-01 00:00:00
abstract::An investigation into the noise levels in a clinical chemistry laboratory has been carried out. It was shown that although the levels were insufficient to cause damage to hearing, they exceeded the criteria of acceptability for offices and workshops. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.23.5.445
更新日期:1970-07-01 00:00:00
abstract::Endocardial fibroelastosis, defined as an endocardium in excess of 30 microns thick, was found in 10 out of 34 cases of hydrops fetalis in a review of 1589 perinatal necropsies carried out between 1976 and 1989. The infants comprised 16 cases of rhesus haemolytic disease, of whom three had endocardial fibroelastosis, ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.44.7.576
更新日期:1991-07-01 00:00:00
abstract::A 57 year old woman presented complaining of increasing abdominal swelling of six months duration. A mixed solid cystic left ovarian tumour measuring 24 cm in diameter was excised. Histology showed numerous cysts lined by benign mucinous epithelium blending imperceptibly into borderline clear cell and mucinous areas t...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.53.12.938
更新日期:2000-12-01 00:00:00
abstract::The incidence of jaundice and of abnormal liver function tests has been assessed in 91 multitransfused patients with severe haemophilia A and B. Tests of hepatocyte function were within the normal range in the majority of patients. On the contrary, tests of biliary cell function, liver cell damage, and bromsulphthalei...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.28.8.620
更新日期:1975-08-01 00:00:00
abstract::Experience gained in the last four years concerning the capabilities and limitations of an 8K Elliott 903 (18-bit word) computer with magnetic tape backing store in the routine operation of a clinical chemistry laboratory is described. Designed as a total system, routine operation has latterly had to be confined to da...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.26.7.480
更新日期:1973-07-01 00:00:00
abstract::Gonococcal isolates resistant to ciprofloxacin collected from 2004 to 2005, in the Pretoria region, were characterised using Neisseria gonorrhoeae multi-antigen sequence typing (NG-MAST). The isolates were obtained from men presenting with urethritis to primary healthcare clinics and general practitioners. All isolate...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2007.052191
更新日期:2008-05-01 00:00:00
abstract::In two cases of infected total hip replacements, Peptococcus magnus was isolated in pure culture from the implant when it was removed. Fluorescent antibody and ELISA studies have shown that both patients developed an antibody response to this anaerobic coccus soon after the replacement operation. These results suggest...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.1.61
更新日期:1979-01-01 00:00:00
abstract::Of 22 patients with Kaposi's sarcoma, 16 had the acquired immune deficiency syndrome (AIDS). The histological pattern in AIDS differs from the more familiar classical Kaposi's sarcoma. The features most useful in making the diagnosis are: dissection of collagen; lymphatic vessel like spaces; angiomatoid lesions; premo...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.39.5.469
更新日期:1986-05-01 00:00:00