Abstract:
AIM:To compare the sensitivity and staining pattern of the new immunohistochemical antibody to tyrosinase (T311) with S-100, HMB45, and the recently evaluated antibody to melan-A (A103) in a range of melanocytic lesions. METHOD:Archival, formalin fixed, paraffin wax embedded sections from 50 benign and malignant melanocytic lesions were stained immunohistochemically with anti-tyrosinase, A103, S-100, and HMB45. They were scored semiquantitatively for the distribution and intensity of staining. RESULTS:All melanomas, with the exception of desmoplastic melanoma, showed some staining with all four antibodies. Overall, T311 and A103 showed an intermediate sensitivity compared with that of S-100 and HMB45. T311 stained most benign and malignant lesions strongly and diffusely with minimal background staining. Immunoreactivity was found to be patchy in some naevi, with weak or absent staining of the mature melanocytes. A103 showed strong and diffuse staining of all benign lesions and most melanomas with minimal background staining. S-100 was the most sensitive, with diffuse staining of most lesions, including desmoplastic and metastatic melanoma, but lacked specificity. HMB45 was the least sensitive antibody, frequently demonstrating patchy staining with absent staining in some benign naevi. CONCLUSIONS:S-100 remains the most sensitive marker of melanocytes. However, because of its lack of specificity, it should be used with at least one other more specific antibody. HMB45 is more specific, but lacks sensitivity; T311 is a reliable marker of melanocytes in paraffin wax embedded sections and is worth consideration for use in a staining panel, although it shows no additional benefit over A103.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Clarkson KS,Sturdgess IC,Molyneux AJdoi
10.1136/jcp.54.3.196keywords:
subject
Has Abstractpub_date
2001-03-01 00:00:00pages
196-200issue
3eissn
0021-9746issn
1472-4146journal_volume
54pub_type
杂志文章abstract:OBJECTIVES:To determine whether antibiotics are prescribed appropriately for acute tonsillitis in an emergency department (ED). METHODS:Cross-sectional observational study in large district general hospital in London. Patients diagnosed and coded with 'acute tonsillitis' in the ED over a 3-month period in 2015. Medica...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2016-203808
更新日期:2016-09-01 00:00:00
abstract::Visual differential counts were examined for efficiency, cost effectiveness, and staff acceptability within our laboratory. A comparison with the Hemalog D system was attempted. The advantages and disadvantages of each system are enumerated and discussed in the context of a large general hospital. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.33.11.1114
更新日期:1980-11-01 00:00:00
abstract:AIM:Solid and papillary epithelial neoplasm (SPEN) is an uncommon pancreatic tumour. Very rarely it has also been described outside the pancreas, usually arising from heterotopic pancreatic tissue. This report summarises all the published extrapancreatic SPENs and documents the sixth such case arising from heterotopic ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.54.3.241
更新日期:2001-03-01 00:00:00
abstract::Strokes are one of the most common causes of mortality and long term severe disability. There is evidence that lipoprotein (a) (Lp(a)) is a predictor of many forms of vascular disease, including premature coronary artery disease. Several studies have also evaluated the association between Lp(a) and ischaemic (thrombot...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.53.7.487
更新日期:2000-07-01 00:00:00
abstract::A total of 104 clinical isolates of Pr. mirabilis from three hospitals were screened for their sensitivity to chlorhexidine. The minimum inhibitory concentrations of the antiseptic for these strains ranged from 10 to 800 mug/ml. Two strains sensitive to 20 mug of chlorhexidine/ml were adapted to resistance by growth i...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.27.4.284
更新日期:1974-04-01 00:00:00
abstract:AIMS:To determine the sensitivity of commercially available diagnostic assays for Chlamydia trachomatis using a simple method. METHODS:Nine commercial assays and an "in-house" polymerase chain reaction (PCR) were evaluated using serial dilutions of a laboratory grown H serovar--four of them using a laboratory grown E ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.5.408
更新日期:1993-05-01 00:00:00
abstract::The proportion of fimbriate strains amongst Escherichia coli freshly isolated from infected urines did not differ significantly from the proportion amongst commensal Esch. coli isolated from the bowel and from non-infected urines. It is concluded that fimbriae play no major role in the pathogenesis of Esch. coli urina...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.20.1.84
更新日期:1967-01-01 00:00:00
abstract::Cyclospora cayetanensis is a cause of prolonged diarrhoea, mainly in travellers. Laboratory diagnosis may be achieved by a number of methods such as the staining of faecal smears by the modified Ziehl-Neelsen (ZN) technique. Safer methods using this technique have been described for the staining of acid fast bacilli a...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.49.6.511
更新日期:1996-06-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.44.12.1007
更新日期:1991-12-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.33.3.282
更新日期:1980-03-01 00:00:00
abstract::Eosinophil polymorphonuclear leucocytes (polymorphs) were counted in 45 specimens from patients with Hodgkin's disease and five specimens from patients with reactive follicular hyperplasia. The use of chlorazol fast pink BK, a little known stain for eosinophil polymorphs, combined with image analysis facilitated rapid...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.3.267
更新日期:1984-03-01 00:00:00
abstract::The incidence of jaundice and of abnormal liver function tests has been assessed in 91 multitransfused patients with severe haemophilia A and B. Tests of hepatocyte function were within the normal range in the majority of patients. On the contrary, tests of biliary cell function, liver cell damage, and bromsulphthalei...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.28.8.620
更新日期:1975-08-01 00:00:00
abstract::Cutaneous melanoma causes most of the skin cancer deaths in the USA. Melanoma is the fifth most common cancer among men and the sixth most common cancer among women. The incidence of melanoma has risen sharply over the past three decades. In this review, which is informed by our extensive experience at a large cancer ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2018-205417
更新日期:2019-04-01 00:00:00
abstract::The sellar region is the site of a large number of pathological entities arising from the pituitary and adjacent anatomical structures, including brain, blood vessels, nerves and meninges. The surgical pathology of this area requires the accurate identification of neoplastic lesions, including pituitary adenoma and ca...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2005.031187
更新日期:2006-12-01 00:00:00
abstract::Guidance by bacteriologists and changes in theatre and ward disciplines have greatly improved the results of urological surgery in recent years. The most noteworthy successes have been in treatment of chronic retention with renal failure, and in prevention of the bacteraemia which may occur following removal of the po...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.17.5.492
更新日期:1964-09-01 00:00:00
abstract::A method for the neutron activation analysis of copper in biological material is described and applied to the diagnosis and management of four cases of Wilson's disease. Results obtained for serum and urine are in agreement with values obtained by established colorimetric techniques. The method described can estimate ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.21.1.8
更新日期:1968-01-01 00:00:00
abstract:AIM:To evaluate a new enzyme immunoassay (EIA) method for detection of Clostridium difficile toxin by comparing it to cytotoxicity assay. To investigate the nature of false negative and false positive EIA results by evaluating clinical and therapeutic parameters. METHODS:737 consecutive diarrhoeal specimens collected ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.50.12.996
更新日期:1997-12-01 00:00:00
abstract:AIMS:Determine whether a simple prewash step will provide adequate amounts of high-quality DNA from core needle biopsies for molecular sequencing studies. METHODS:The quantitative and qualitative metrics of DNA recovered from core needle biopsies processed either by 1) formalin fixation and paraffin embedding (FFPE), ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2016-204061
更新日期:2017-06-01 00:00:00
abstract:AIMS:To determine the sensitivity and specificity of a multiplex PCR assay for the contemporary identification of major species involved in oral candidiasis, without extraction and purification of DNA from the samples under investigation; and evaluation of this method in comparison with routine phenotypic culture ident...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2006.040782
更新日期:2007-09-01 00:00:00
abstract::Human oxalated plasma stored at 4 degrees C. until the prothrombin time is increased beyond 60 sec. is a reliable medium for assaying labile factor (factor V) because its response to added labile factor corresponds quantitatively to that of plasma from patients with congenital deficiency of this factor. Such an agreem...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.13.6.457
更新日期:1960-11-01 00:00:00
abstract::The cellularity of 104 primary breast carcinomata was determined by semiautomated image analysis to allow the relation between cellularity and oestrogen receptor content values to be assessed. The oestrogen receptor content of tumours detected by a steroid binding assay showed no correlation with cellularity, although...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.11.1166
更新日期:1989-11-01 00:00:00
abstract:BACKGROUND/AIMS:A twofold increased risk for breast cancer has been reported recently for women with late onset diabetes. Most studies showed that there were differences in serum concentrations of insulin-like growth factors and related proteins between women with and without diabetes who have breast cancer. This study...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.56.8.599
更新日期:2003-08-01 00:00:00
abstract::It was postulated that the short chain fatty acids (SCFA) produced by anaerobic bacteria might serve as microbial markers in purulent material. Eighteen pus specimens from various sources were analysed by gas-liquid chromatography (GLC), and the SCFA detected were compared with the microorganisms isolated by conventio...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.11.1163
更新日期:1979-11-01 00:00:00
abstract:AIM:Lymphocytic infiltration in the portal triads usually conceals the detection--in haematoxylin and eosin (H&E) stained sections--of bile ducts in two liver diseases: chronic hepatitis and primary biliary cirrhosis. The aim was to assess the number and the characteristics of the bile ducts in those diseases with the ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.53.10.765
更新日期:2000-10-01 00:00:00
abstract:AIMS:To evaluate the effect of microwave irradiation on the staining quality of a range of commonly used primary antibodies in archival, formalin fixed, paraffin wax embedded material, with emphasis on antibodies that have previously worked successfully only on frozen tissue. METHODS:Immunocytochemistry (streptavidin-...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.5.448
更新日期:1994-05-01 00:00:00
abstract:AIMS:To audit the cellular expression of the innate antibacterial enzyme lysozyme in colonic biopsies from a cohort of patients having microscopic colitis (MC-collagenous colitis (CC) or lymphocytic colitis (LC)). Results were compared with those recorded in patients with inflammatory bowel disease (IBD) of the colon (...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2010.086850
更新日期:2011-06-01 00:00:00
abstract:AIM:Recent events surrounding postmortem practices have necessitated several changes in the way necropsies are conducted both within the medicolegal and hospital settings. These changes have resulted in a striking reduction in the frequency of brain retention for neuropathological examination. Aberdeen Royal Infirmary,...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.028274
更新日期:2006-04-01 00:00:00
abstract::Mallory bodies were isolated from necropsy livers from patients with alcoholic hepatitis with and without cirrhosis with a Ficoll viscosity barrier. The purity of Mallory bodies in the isolate varied between 70 and 90%, estimated by counting Mallory bodies and non-Mallory body structures in haematoxylin-eosin stained ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.9.1010
更新日期:1981-09-01 00:00:00
abstract:AIM:To develop a polymerase chain reaction (PCR) for the specific detection of the C protein gene in strains of group B Streptococcus. METHODS:A single primer pair derived from the nucleotide sequence of the IgA binding beta antigen of the C protein complex permitted the specific amplification of a 592 base pair DNA f...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.7.633
更新日期:1993-07-01 00:00:00
abstract:AIM:To determine whether the pattern of involvement of the gastric mucosa in lymphocytic gastritis is predictive of the presence or absence of duodenal pathology. METHODS:50 cases (M:F, 26:24; median age 57 years) diagnosed as lymphocytic gastritis between 1986 and 1998 with concurrent duodenal (D2) biopsies were iden...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.52.11.815
更新日期:1999-11-01 00:00:00