Abstract:
AIMS:Lung cancer predictive biomarker testing is essential to select advanced-stage patients for targeted treatments and should be carried out without delays even during health emergencies, such as the coronavirus (COVID-19) outbreak. METHODS:Fifteen molecular laboratories from seven different European countries compared 4 weeks of national lockdown to a corresponding period in 2019, in terms of tissue and/or plasma-based molecular test workload, analytical platforms adopted, number of cases undergoing programmed death-ligand1 (PD-L1) expression assessment and DNA-based molecular tests turnaround time. RESULTS:In most laboratories (80.0%), tissue-based molecular test workload was reduced. In 40.0% of laboratories (6/15), the decrease was >25%, and in one, reduction was as high as 80.0%. In this instance, a concomitant increase in liquid biopsy was reported (60.0%). Remarkably, in 33.3% of the laboratories, real-time PCR (RT-PCR)-based methodologies increased, whereas highly multiplexing assays approaches decreased. Most laboratories (88.9%) did not report significant variations in PD-L1 volume testing. CONCLUSIONS:The workload of molecular testing for patients with advanced-stage lung cancer during the lockdown showed little variations. Local strategies to overcome health emergency-related issues included the preference for RT-PCR tissue-based testing methodologies and, occasionally, for liquid biopsy.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Malapelle U,Pisapia P,Iaccarino A,Barberis M,Bellevicine C,Brunnström H,de Biase D,De Maglio G,Ericson Lindquist K,Fassan M,Fontanini G,Gruppioni E,Hofman P,Merkelbach-Bruse S,Molina Vila MA,Pujals A,Rapa I,Righi L,Rodoi
10.1136/jclinpath-2020-206957subject
Has Abstractpub_date
2020-07-31 00:00:00eissn
0021-9746issn
1472-4146pii
jclinpath-2020-206957pub_type
杂志文章abstract:AIMS:To test whether a hypoacidic environment may potentially "stress" Helicobacter pylori DNA, encouraging the emergence of strain variation. METHODS:This hypothesis was tested by inducing prolonged hypoacidity with omeprazole, a potent antisecretory drug. The genomic DNA of H pylori was studied by electrophoretic se...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.45.12.1062
更新日期:1992-12-01 00:00:00
abstract:AIM:To evaluate a new enzyme immunoassay (EIA) method for detection of Clostridium difficile toxin by comparing it to cytotoxicity assay. To investigate the nature of false negative and false positive EIA results by evaluating clinical and therapeutic parameters. METHODS:737 consecutive diarrhoeal specimens collected ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.50.12.996
更新日期:1997-12-01 00:00:00
abstract:AIMS:To determine the sensitivity and specificity of a multiplex PCR assay for the contemporary identification of major species involved in oral candidiasis, without extraction and purification of DNA from the samples under investigation; and evaluation of this method in comparison with routine phenotypic culture ident...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2006.040782
更新日期:2007-09-01 00:00:00
abstract::Review of published work and analysis of clinical data and pathology of four biopsy specimens from two patients with laryngeal contact granuloma showed that its peptic origin was derived from a gastro-oesophago-laryngeal reflux. It is proposed that the term "peptic granuloma" should be given to this phenomenon. This t...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.8.800
更新日期:1989-08-01 00:00:00
abstract::A quantitative histological analysis of the major salivary glands was carried out at necropsy in 28 alcoholics and in a series of age and sex matched controls. The findings were related to the different types of histologically diagnosed liver disease present. Significant quantitative changes of salivary gland structur...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.8.837
更新日期:1988-08-01 00:00:00
abstract:BACKGROUND/AIMS:Mast cells, when activated, secrete a large number of fibrogenic factors and have been implicated in the development of fibrotic conditions of the liver, lung, and skin. There is evidence that renal fibrosis is closely linked with a chronic inflammatory cell infiltrate within the interstitium, but a pot...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.53.11.858
更新日期:2000-11-01 00:00:00
abstract::Guinea-pigs, rats, dogs, rabbits, monkeys, sheep, and goats were screened for G-6PD activity in erythrocytes by the methaemoglobin reduction test, the brilliant cresyl blue decolorization test, and the spectrophotometric determination of the NADPH generation rate. Guinea-pigs, rats, dogs, rabbits, and monkeys were fou...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.19.6.614
更新日期:1966-11-01 00:00:00
abstract:AIMS:To describe the prostatic adenectomy specimens of six patients with symptomatic benign prostatic hyperplasia (BPH) who failed to respond to long term treatment with a 5-alpha-reductase inhibitor, finasteride. METHODS:Histological sections from six cases of BPH who had been treated with finasteride were investigat...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.49.4.324
更新日期:1996-04-01 00:00:00
abstract:AIMS:To audit the cellular expression of the innate antibacterial enzyme lysozyme in colonic biopsies from a cohort of patients having microscopic colitis (MC-collagenous colitis (CC) or lymphocytic colitis (LC)). Results were compared with those recorded in patients with inflammatory bowel disease (IBD) of the colon (...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2010.086850
更新日期:2011-06-01 00:00:00
abstract::Aztreonam blood agar, a new selective medium for Gram positive aerobic bacteria, was evaluated in comparison with conventional media for skin swabs. Aztreonam agar increased the number of isolates of Staphylococcus aureus by 17%. By producing purer growths on primary isolation, it significantly speeded up the identifi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.8.769
更新日期:1993-08-01 00:00:00
abstract::Components of the blood fibrinolytic system were measured in 43 patients with leukaemia. Increased fibrinolytic activity, indicated by a reduced plasma plasminogen level, was found in 14 patients. The combination of severe thrombocytopenia and increased fibrinolysis appeared to be a determinant in the production of a ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.21.2.136
更新日期:1968-03-01 00:00:00
abstract::Distinctly increased levels of antibodies to measles virus envelope antigens haemolysin and haemagglutinin were found in the sera of patients with chronic active hepatitis compared with a normal control group, using immunofluorescence and functional tests. Similarly, a higher incidence of smooth muscle antibody of bot...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.11.1281
更新日期:1985-11-01 00:00:00
abstract::In order to detect the heat labile toxin of Escherichia coli human embryo lung fibroblast cells were seeded with whole cell lysate preparations of the organism to be tested. Positive results consisted of growth inhibition and cytopathic change which were easily seen. Heat-labile toxin was produced by strains belonging...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.12.1216
更新日期:1979-12-01 00:00:00
abstract::As part of a study to establish the prevalence of renal calculus disease, alkaline phosphatase activity was measured by the method recommended by the Scandinavian Society for Clinical Chemistry and Clinical Physiology in the plasma of 3823 adults from the Scottish new town of Cumbernauld. The enzyme activity differs i...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.12.1202
更新日期:1978-12-01 00:00:00
abstract::The costs of a clinical chemistry laboratory in a district general hospital were studied. The system used has certain advantages over the conventional Cooper Lybrand method. The time taken by technicians to perform tests was more variable than expected and the cost of sample collection was higher than process-cost for...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.6.589
更新日期:1981-06-01 00:00:00
abstract:AIMS:To institute recommendations from a laboratory turnround time study; to evaluate audit methods; and to quantify improvements achieved. METHODS:Changes to result report distribution and specimen delivery were affected by posting results directly from the laboratory followed by the introduction of a twice daily cou...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.4.372
更新日期:1995-04-01 00:00:00
abstract::Synovium was collected from 15 patients who were undergoing joint surgery. Two groups were defined by clinical diagnosis: patients with primary osteoarthritis (n = 4); and those with rheumatoid arthritis (n = 11). The synovium was studied using histological and morphometric techniques. In agreement with previous studi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.8.847
更新日期:1988-08-01 00:00:00
abstract::Gastroblastoma is a rare gastric epitheliomesenchymal biphasic tumour composed of spindle and epithelial cells, reported by Miettinen et al in a series of three cases in 2009. All those cases arose in stomachs of young adults. Neither the epithelial nor the mesenchymal component displayed sufficient atypia to diagnose...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2009.074104
更新日期:2010-03-01 00:00:00
abstract:AIMS:To determine important factors influencing recurrence after local excision of duct carcinoma in situ (DCIS) of the breast. MATERIALS AND METHODS:The extent (size) in millimetres, classification (by cytonuclear grade (NHSBSP system), by extent of necrosis, and by the Van Nuys system), and excision margins of 115 c...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.55.8.581
更新日期:2002-08-01 00:00:00
abstract::A technique is described whereby inclusion-bearing cells identified by light microscopy in stained smears of urinary sediment were reprocessed for examination in the electron microscope. The nuclei of the abnormal cells were found to contain numerous virus particles, 35 nm in diameter, which morphologically resembled ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.30.11.1015
更新日期:1977-11-01 00:00:00
abstract::Liver function was assessed in 38 Edinburgh haemophiliacs. Results before the introduction of NHS intermediate factor VIII concentrate from 1974 onwards were compared with values in 1979. Measurements of serum bile salts in 16 patients as well as conventional liver function tests gave useful evidence of deranged liver...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.1.17
更新日期:1981-01-01 00:00:00
abstract::This is a retrospective study of the results of 4,642 blood cultures taken from 2,190 patients from 1960 to 1967 in one London hospital. The results suggested that an increased incidence of bacteraemias at this particular hospital was mainly due to the increase in the number of patients having blood cultures. Some of ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.23.2.166
更新日期:1970-03-01 00:00:00
abstract::A rare case of adult onset Langerhans' cell histiocytosis associated with dysgerminoma in a 35 year old Chinese woman is reported. The patient had a history of dysgerminoma of left ovary 15 years previously and had undergone surgery followed by radiotherapy and an uneventful recovery. She presented again in March 1994...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.10.963
更新日期:1995-10-01 00:00:00
abstract::CAM 5.2 is a murine monoclonal antibody, raised against the colon carcinoma cell line HT29, which recognises lower molecular weight intracellular cytokeratin proteins within secretory epithelia. Extensive indirect immunohistochemical studies have confirmed that this antibody stains formalin fixed (and freshly frozen) ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.9.975
更新日期:1984-09-01 00:00:00
abstract:AIMS:To evaluate two detailed step sectioning protocols for sentinel lymph nodes (SLNs). METHODS:After vital dye or combined dye and radiocolloid guided biopsy, SLNs were fixed in formalin and embedded in paraffin wax. In protocol A, SLNs from 123 patients were sectioned in steps of 50-100 micro m, whereas in protocol...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.55.12.926
更新日期:2002-12-01 00:00:00
abstract:AIMS:To study the incidence, investigation, and management of severe hyponatraemia (serum sodium < 120 mmol/litre) over a period of six months in a district general hospital. METHODS:The laboratory computer was used to identify all inpatients who had a serum sodium concentration of less than 120 mmol/litre over a six ...
journal_title:Journal of clinical pathology
pub_type: 评论,杂志文章
doi:10.1136/jcp.55.12.893
更新日期:2002-12-01 00:00:00
abstract::Small cell neuroendocrine carcinoma of the breast is a rare tumour with less than 30 cases reported in the literature. The clinicopathological findings of three cases of primary neuroendocrine carcinoma of the breast and a review of the pertinent literature are presented. The morphological and immunohistochemical patt...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2004.020792
更新日期:2005-07-01 00:00:00
abstract::A 60 year old woman affected by Hashimoto's thyroiditis presented with a history of recurring episodes of urticaria and angio-oedema. Clinical and laboratory evaluation of the patient excluded allergy to external agents, hereditary angio-oedema, and occult infections. A pathogenic relation between Hashimoto's thyroidi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.50.3.254
更新日期:1997-03-01 00:00:00
abstract::A recently developed procedure, that has been shown to be suitable for detailed immunohistological analysis, has been used to prepare cryostat sections of bone marrow to investigate whether enzyme-histochemical techniques are also feasible on such material. A selected group of enzymes, some of which are inhibited or d...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.35.11.1220
更新日期:1982-11-01 00:00:00
abstract::A patient with rhabdomyosarcoma is described in whom the presenting clinical and laboratory features were those of disseminated intravascular coagulation. The patient's rapid downhill course was primarily expressed by haemorrhagic tendency. An alveolar rhabdomyosarcoma, affecting many organs, including vascular and ca...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.30.7.661
更新日期:1977-07-01 00:00:00