Production of a functional eukaryotic enzyme in Escherichia coli: cloning and expression of the yeast structural gene for imidazole-glycerolphosphate dehydratase (his3).

abstract：:In the mammalian retina, extensive processing of spatiotemporal and chromatic information occurs. One key principle in signal transfer through the retina is parallel processing. Two of these parallel pathways are the ON- and OFF-channels transmitting light and dark signals. This dual system is created in the outer ple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hack I,Peichl L,Brandstätter JH

更新日期：1999-11-23 00:00:00

abstract：:Protein misfolding and aggregation cause serious degenerative conditions such as Alzheimer's, Parkinson, and prion diseases. Damage to membranes is thought to be one of the mechanisms underlying cellular toxicity of a range of amyloid assemblies. Previous studies have indicated that amyloid fibrils can cause membrane ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Milanesi L,Sheynis T,Xue WF,Orlova EV,Hellewell AL,Jelinek R,Hewitt EW,Radford SE,Saibil HR

更新日期：2012-12-11 00:00:00

abstract：:Subviral particles containing reovirus RNA transcriptase have been isolated from extracts of virus-infected mouse fibroblast cells. The purified particles which lacked the outer protein capsomeres of the mature virion had a buoyant density of 1.43-1.44 g/ml in CsCl and contained all of the double-stranded RNA genome o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levin DH,Mendelsohn N,Schonberg M,Klett H,Silverstein S,Kapuler AM,Acs G

更新日期：1970-07-01 00:00:00

abstract：:Terminal oligopyrimidine (TOP) motifs are sequences at the 5' ends of mRNAs that link their translation to the mTOR Complex 1 (mTORC1) nutrient-sensing signaling pathway. They are commonly regarded as discrete elements that reside on ∼100 mRNAs that mostly encode translation factors. However, the full spectrum of TOP ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Philippe L,van den Elzen AMG,Watson MJ,Thoreen CC

更新日期：2020-03-10 00:00:00

abstract：:Zif268, a zinc finger protein whose mRNA is rapidly activated in cells exposed to growth factors or other signaling agents, is thought to play a role in regulating the genetic program induced by extracellular ligands. We report that Zif268 has one of the characteristics of a transcriptional regulator, namely, sequence...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Christy B,Nathans D

更新日期：1989-11-01 00:00:00

abstract：:Plants frequently respond to herbivorous insect attack by synthesizing defense proteins that deter insect feeding and prevent additional herbivory. Maize (Zea mays L.) lines, resistant to feeding by a number of lepidopteran species, rapidly mobilize a unique 33-kDa cysteine protease in response to caterpillar feeding....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pechan T,Cohen A,Williams WP,Luthe DS

更新日期：2002-10-01 00:00:00

abstract：:The murine-virus-infected erythroleukemia cell system provides an opportunity to examine regulatory mechanisms controlling cytodifferentiation. A cloned cell line (DR10c3) resistant to the erythropoiesis-inducing effect of dimethylsulfoxide (Me2SO) was isolated from the Me2SO-sensitive line DS19. DR10c3 is characteriz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohta Y,Tanaka M,Terada M,Miller OJ,Bank A,Marks P,Rifkind RA

更新日期：1976-04-01 00:00:00

abstract：:Metallothionein (MT) has several putative roles in metal detoxification, in Zn and Cu homeostasis, in scavenging free radicals, and in the acute phase response. Mice of mixed 129/Ola and C57BL/6J background with targeted disruption of MT-I and MT-II genes are more sensitive to toxic metals and oxidative stress. We not...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beattie JH,Wood AM,Newman AM,Bremner I,Choo KH,Michalska AE,Duncan JS,Trayhurn P

更新日期：1998-01-06 00:00:00

abstract：:In genotypic mutation analysis DNA sequence changes are determined without the in vivo or in vitro selection of phenotypically altered cells. We have studied the induction of base-pair changes by N-ethyl-N-nitrosourea in Taq I endonuclease recognition site 2508-2511 (TCGA) of the c-H-ras1 gene in human fibroblasts by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chiocca SM,Sandy MS,Cerutti PA

更新日期：1992-06-15 00:00:00

abstract：:Mutations at position 187 in secreted gelsolin enable aberrant proteolysis at the 172-173 and 243-244 amide bonds, affording the 71-residue amyloidogenic peptide deposited in Familial Amyloidosis of Finnish Type (FAF). Thermodynamic comparisons of two different domain 2 constructs were carried out to study possible ef...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ratnaswamy G,Huff ME,Su AI,Rion S,Kelly JW

更新日期：2001-02-27 00:00:00

abstract：:Anthrax lethal toxin (LT) is a protease virulence factor produced by Bacillus anthracis that is required for its pathogenicity. LT treatment causes a rapid degradation of c-Jun protein that follows inactivation of the MEK1/2-Erk1/2 signaling pathway. Here we identify COP1 as the ubiquitin E3 ligase that is essential f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ouyang W,Guo P,Takeda K,Fu Q,Fang H,Frucht DM

更新日期：2020-02-25 00:00:00

abstract：:The release of catecholamines from adrenal chromaffin cells is known to be blocked by dihydropyridines, such as nitrendipine, and enhanced by others, such as BAY K8644. On the other hand, release from sympathetic neurons is predominantly insensitive to these agents. Release of [3H]norepinephrine from undifferentiated ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kongsamut S,Miller RJ

更新日期：1986-04-01 00:00:00

abstract：:Compared with the U.S., Japan is believed to have a collectivist culture that nurtures high trust. Results from laboratory and survey research, however, show that Americans are more likely to trust strangers than are Japanese. Why would trust be lower in a collectivist culture? We use an agent-based computational mode...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Macy MW,Sato Y

更新日期：2002-05-14 00:00:00

abstract：:This article considers replicability of the performance of predictors across studies. We suggest a general approach to investigating this issue, based on ensembles of prediction models trained on different studies. We quantify how the common practice of training on a single study accounts in part for the observed chal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patil P,Parmigiani G

更新日期：2018-03-13 00:00:00

abstract：:Substantial proportions of mammalian genomes comprise repetitive elements including endogenous retrotransposons. Although these play diverse roles during development, their appropriate silencing is critically important in maintaining genomic integrity in the host cells. The major mechanism for retrotransposon silencin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hatanaka Y,Inoue K,Oikawa M,Kamimura S,Ogonuki N,Kodama EN,Ohkawa Y,Tsukada Y,Ogura A

更新日期：2015-11-24 00:00:00

abstract：:In the absence of adaptive immunity displayed by animals, plants respond locally to biotic challenge via inducible basal defense networks activated through recognition and response to conserved pathogen-associated molecular patterns. In addition, immunity can be induced in tissues remote from infection sites by system...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Truman W,Bennett MH,Kubigsteltig I,Turnbull C,Grant M

更新日期：2007-01-16 00:00:00

abstract：:The transition to pulmonary respiration after birth requires rapid alterations in the structure of the mammalian cardiovascular system. One dramatic change that occurs is the closure of the ductus arteriosus (DA), an arterial connection in the fetus that directs blood flow away from the pulmonary circulation. Two memb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levet S,Ouarné M,Ciais D,Coutton C,Subileau M,Mallet C,Ricard N,Bidart M,Debillon T,Faravelli F,Rooryck C,Feige JJ,Tillet E,Bailly S

更新日期：2015-06-23 00:00:00

abstract：:Ecosystem services are supplied by nature but, by definition, are received by people. Ecosystem service assessments, intended to influence the decisions people make regarding their interactions with nature, need to understand how people benefit from different ecosystem services. A critical question is therefore, What ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hicks CC,Cinner JE

更新日期：2014-12-16 00:00:00

abstract：:Exposures to ambient and household fine-particulate matter (PM2.5) together are among the largest single causes of premature mortality in India according to the Global Burden of Disease Studies (GBD). Several recent investigations have estimated that household emissions are the largest contributor to ambient PM2.5 exp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chowdhury S,Dey S,Guttikunda S,Pillarisetti A,Smith KR,Di Girolamo L

更新日期：2019-05-28 00:00:00

abstract：:Huntington disease (HD) is caused by an expansion mutation of the N-terminal polyglutamine of huntingtin (mHTT). mHTT is ubiquitously present, but it induces noticeable damage to the brain's striatum, thereby affecting motor, psychiatric, and cognitive functions. The striatal damage and progression of HD are associate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sharma M,Rajendrarao S,Shahani N,Ramírez-Jarquín UN,Subramaniam S

更新日期：2020-07-07 00:00:00

abstract：:The composition of biologic molecules isolated from biominerals suggests that control of mineral growth is linked to biochemical features. Here, we define a systematic relationship between the ability of biomolecules in solution to promote the growth of calcite (CaCO3) and their net negative molecular charge and hydro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elhadj S,De Yoreo JJ,Hoyer JR,Dove PM

更新日期：2006-12-19 00:00:00

abstract：:The ability to instantly create a state of immunity as achieved in the passive transfer of hyperimmune globulin has had a tremendous impact on public health. Unlike passive immunization, active immunization, which is the foundation of vaccinology, is an anticipatory strategy with inherent limitations. Here we show tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Popkov M,Gonzalez B,Sinha SC,Barbas CF 3rd

更新日期：2009-03-17 00:00:00

abstract：:The substance P (SP)-preferring receptor neurokinin-1 receptor (NK-1R) has two forms: a full-length receptor consisting of 407 aa and a truncated receptor consisting of 311 aa. These two receptors differ in the length of the C terminus of NK-1R. We studied the undifferentiated and phorbol myristate acetate (PMA)-diffe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lai JP,Ho WZ,Kilpatrick LE,Wang X,Tuluc F,Korchak HM,Douglas SD

更新日期：2006-05-16 00:00:00

abstract：:Phylogenetic analysis of Plasmodium parasites has indicated that their modern-day distribution is a result of a series of human-mediated dispersals involving transport between Africa, Europe, America, and Asia. A major outstanding question is the phylogenetic affinity of the malaria causing parasites Plasmodium vivax ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gelabert P,Sandoval-Velasco M,Olalde I,Fregel R,Rieux A,Escosa R,Aranda C,Paaijmans K,Mueller I,Gilbert MT,Lalueza-Fox C

更新日期：2016-10-11 00:00:00

abstract：:Enhanced ethylene production is an early response of plants to pathogen attack and has been associated with both resistance and susceptibility to disease. Tobacco plants were transformed with the mutant etr1-1 gene from Arabidopsis, conferring dominant ethylene insensitivity. Besides lacking known ethylene responses, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Knoester M,van Loon LC,van den Heuvel J,Hennig J,Bol JF,Linthorst HJ

更新日期：1998-02-17 00:00:00

abstract：:The N-terminal domain of HIV-1 integrase contains a pair of His and Cys residues (the HHCC motif) that are conserved among retroviral integrases. Although His and Cys residues are often involved in binding zinc, the HHCC motif does not correspond to any recognized class of zinc binding domain. We have investigated the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zheng R,Jenkins TM,Craigie R

更新日期：1996-11-26 00:00:00

abstract：:Neurofibromin gene (NF1) mutation causes neurofibromatosis type 1 (NF1), a disorder in which brain white matter deficits identified by neuroimaging are common, yet of unknown cellular etiology. In mice, Nf1 loss in adult oligodendrocytes causes myelin decompaction and increases oligodendrocyte nitric oxide (NO) levels...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Asleh J,Shofty B,Cohen N,Kavushansky A,López-Juárez A,Constantini S,Ratner N,Kahn I

更新日期：2020-09-08 00:00:00

abstract：:Water dynamics in concentrated ionic solutions plays an important role in a number of material and energy conversion processes such as the charge transfer at the electrolyte-electrode interface in aqueous rechargeable ion batteries. One long-standing puzzle is that all electrolytes, regardless of their "structure-maki...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Q,Wu T,Chen C,Mukamel S,Zhuang W

更新日期：2017-09-19 00:00:00

abstract：:In response to hypoxia the hypoxia-inducible factor-1 (HIF-1) mediates transcriptional activation of a network of genes encoding erythropoietin, vascular endothelial growth factor, and several glycolytic enzymes. HIF-1 consists of a heterodimer of two basic helix-loop-helix PAS (Per/Arnt/Sim) proteins, HIF-1alpha and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kallio PJ,Pongratz I,Gradin K,McGuire J,Poellinger L

更新日期：1997-05-27 00:00:00

abstract：:The number of red blood cells is normally tightly regulated by a classic homeostatic mechanism based on oxygen sensing in the kidney. Decreased oxygen delivery resulting from anemia induces the production of erythropoietin, which increases red cell production and hence oxygen delivery. Investigations of erythropoietin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Percy MJ,Zhao Q,Flores A,Harrison C,Lappin TR,Maxwell PH,McMullin MF,Lee FS

更新日期：2006-01-17 00:00:00