Abstract：
:Injury and loss of oligodendrocytes can cause demyelinating diseases such as multiple sclerosis. To improve our understanding of human oligodendrocyte development, which could facilitate development of remyelination-based treatment strategies, here we describe time-course single-cell-transcriptomic analysis of developing human stem cell-derived oligodendrocyte-lineage-cells (hOLLCs). The study includes hOLLCs derived from both genome engineered embryonic stem cell (ESC) reporter cells containing an Identification-and-Purification tag driven by the endogenous PDGFRα promoter and from unmodified induced pluripotent (iPS) cells. Our analysis uncovers substantial transcriptional heterogeneity of PDGFRα-lineage hOLLCs. We discover sub-populations of human oligodendrocyte progenitor cells (hOPCs) including a potential cytokine-responsive hOPC subset, and identify candidate regulatory genes/networks that define the identity of these sub-populations. Pseudotime trajectory analysis defines developmental pathways of oligodendrocytes vs astrocytes from PDGFRα-expressing hOPCs and predicts differentially expressed genes between the two lineages. In addition, pathway enrichment analysis followed by pharmacological intervention of these pathways confirm that mTOR and cholesterol biosynthesis signaling pathways are involved in maturation of oligodendrocytes from hOPCs.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Chamling X,Kallman A,Fang W,Berlinicke CA,Mertz JL,Devkota P,Pantoja IEM,Smith MD,Ji Z,Chang C,Kaushik A,Chen L,Whartenby KA,Calabresi PA,Mao HQ,Ji H,Wang TH,Zack DJdoi
10.1038/s41467-021-20892-3subject
Has Abstractpub_date
2021-01-28 00:00:00pages
652issue
1issn
2041-1723pii
10.1038/s41467-021-20892-3journal_volume
12pub_type
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