Abstract：
:Cell-free DNA is present in different biological fluids and when released by tumor cells may contribute to pro-tumor events such as malignant transformation of cells adjacent to the tumor and metastasis. Thus, this study analyzed the effect of tumor cell-free DNA, isolated from the blood of prostate cancer patients, on non-tumor prostate cell lines (RWPE-1 and PNT-2). To achieve this, we performed cell-free DNA quantification and characterization assays, evaluation of gene and miRNA expression profiling focused on cancer progression and EMT, and metabolomics by mass spectrometry and cellular migration. The results showed that tumor-free cell DNA was able to alter the gene expression of MMP9 and CD44, alter the expression profile of nine miRNAs, and increased the tryptophan consumption and cell migration rates in non-tumor cells. Therefore, tumor cell-free DNA was capable of altering the receptor cell phenotype, triggering events related to malignant transformation in these cells, and can thus be considered a potential target for cancer diagnosis and therapy.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Souza AG,Bastos VAF,Fujimura PT,Ferreira ICC,Leal LF,da Silva LS,Laus AC,Reis RM,Martins MM,Santos PS,Corrêa NCR,Marangoni K,Thomé CH,Colli LM,Goulart LR,Goulart VAdoi
10.1038/s41598-020-78766-5subject
Has Abstractpub_date
2020-12-10 00:00:00pages
21674issue
1issn
2045-2322pii
10.1038/s41598-020-78766-5journal_volume
10pub_type
杂志文章abstract：:Microphysiological systems (MPSs) are in vitro models that capture facets of in vivo organ function through use of specialized culture microenvironments, including 3D matrices and microperfusion. Here, we report an approach to co-culture multiple different MPSs linked together physiologically on re-useable, open-syste...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22749-0
更新日期：2018-03-14 00:00:00
abstract：:Canadian Indigenous peoples (First Nations and Inuit) exhibit a high burden of infectious diseases including tuberculosis influenced by societal factors, and biological determinants. Toll-like receptor (TLR)-mediated innate immune responses are the first line of defence against infections. We examined the production o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50596-0
更新日期：2019-10-02 00:00:00
abstract：:Omniphobic surfaces with reentrant microstructures have been investigated for a range of applications, but the evaporation of high- and low-surface-tension liquid droplets placed on such surfaces has not been rigorously studied. In this work, we develop a technique to fabricate omniphobic surfaces on copper substrates...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17110
更新日期：2015-11-25 00:00:00
abstract：:A fast Endospore Germinability Assay (EGA) was validated with traditional plate counts to enumerate single endospore germination events for monitoring surface sterilization. The assay is based on a time-gated luminescence microscopy technique enabling visualization and enumeration of individual germinating endospores....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-57175-3
更新日期：2020-01-16 00:00:00
abstract：:Gravity perception plays a key role in how plants develop and adapt to environmental changes. However, more than a century after the pioneering work of Darwin, little is known on the sensing mechanism. Using a centrifugal device combined with growth kinematics imaging, we show that shoot gravitropic responses to stead...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35431
更新日期：2016-10-14 00:00:00
abstract：:In this study, we report a microfluidic device for the whole-life culture of the nematode Caenorhabditis elegans that allows the scoring of animal survival and health measures. This device referred to as the NemaLife chip features: (1) an optimized micropillar arena in which animals can crawl, (2) sieve channels that ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73002-6
更新日期：2020-10-01 00:00:00
abstract：:Short-wavelength luminescence is essential for high-performance optoelectronic device applications. There have been efforts to obtain intense ultraviolet (UV) emission by encapsulating ZnO one-dimensional (1D) nanostructures with materials such as ZnS. However, the encapsulation of ZnS 1D nanostructures with ZnO has n...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13556-0
更新日期：2017-10-12 00:00:00
abstract：:Impulsivity is a naturally occurring behavior that, when accentuated, can be found in a variety of neuropsychiatric disorders. The expression of trait impulsivity has been shown to change with a variety of factors, such as age and sex, but the existing literature does not reflect widespread consensus regarding the inf...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35537-7
更新日期：2018-12-05 00:00:00
abstract：:Alterations in the gut microbiome have been reported in liver cirrhosis, and probiotic interventions are considered a potential treatment strategy. This study aimed to evaluate the effects and mechanisms of Lactobacillus salivarius LI01, Pediococcus pentosaceus LI05, Lactobacillus rhamnosus GG, Clostridium butyricum M...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07091-1
更新日期：2017-07-31 00:00:00
abstract：:Chitin is a valuable peat substrate amendment by increasing lettuce growth and reducing the survival of the zoonotic pathogen Salmonella enterica on lettuce leaves. The production of chitin-catabolic enzymes (chitinases) play a crucial role and are mediated through the microbial community. A higher abundance of plant-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46106-x
更新日期：2019-07-09 00:00:00
abstract：:Ultracentrifugationon sucrose density gradientappears to be the best purification protocol for extracellular vesicle (EVs) purification. After this step, to reduce disulfide bridges linking exogenous proteins to the vesicles, the collected samples are routinely washed and treated with dithiothreitol (DTT). Such incuba...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47598-3
更新日期：2019-09-10 00:00:00
abstract：:The current theories of antimalarial mechanism of artemisinin are inadequate to fully explain the observed effects. In our study, "organism-like" formation of Schistosoma hemozoin granules by attaching to and utilizing erythrocytes to form new ones was observed. This indicates that heme iron is transferred from erythr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34463
更新日期：2016-10-03 00:00:00
abstract：:Horseradish peroxidase (HRP) mediates efficient conversion of many phenolic contaminants and thus has potential applications for pollution control. Such potentially important applications suffer however from the fact that the enzyme becomes quickly inactivated during phenol oxidation and polymerization. The work here ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03126
更新日期：2013-11-04 00:00:00
abstract：:In this work we have studied the effects of pharmacological concentrations of melatonin (1 µM-1 mM) on pancreatic stellate cells (PSC). Cell viability was analyzed by AlamarBlue test. Production of reactive oxygen species (ROS) was monitored following CM-H2DCFDA and MitoSOX Red-derived fluorescence. Total protein carb...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63433-6
更新日期：2020-04-14 00:00:00
abstract：:We have found a stable spin-structure of N [Formula: see text] 3 system in which three spin-3 atoms are trapped and coupled to total spin S [Formula: see text] 3. We have proved that a pair of this structure is nearly an exact solution for N [Formula: see text] 6 systems in a very broad district in the parameter-space...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81133-7
更新日期：2021-01-19 00:00:00
abstract：:Global lake systems have undergone rapid degradation over the past century. Scientists and managers are struggling to manage the highly degraded lake systems to cope with escalating anthropogenic pressures. Improved knowledge of how lakes and social systems co-evolved up to the present is vital for understanding, mode...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35482-5
更新日期：2018-11-21 00:00:00
abstract：:We examined whether it is possible to identify individual subjects on the basis of brain anatomical features. For this, we analyzed a dataset comprising 191 subjects who were scanned three times over a period of two years. Based on FreeSurfer routines, we generated three datasets covering 148 anatomical regions (corti...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23696-6
更新日期：2018-04-04 00:00:00
abstract：:Combined neutron scattering and diffusion nuclear magnetic resonance experiments have been used to reveal significant interregional asymmetries (lateralization) in bovine brain hemispheres in terms of myelin arrangement and water dynamics at micron to atomic scales. Thicker myelin sheaths were found in the left hemisp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51022-1
更新日期：2019-10-11 00:00:00
abstract：:This study aimed to evaluate the effect of brain atrophy on the functional outcome of patients with moderate-volume basal ganglia hemorrhage. Of 1003 patients with spontaneous intracerebral hemorrhage, 124 with moderate-volume basal ganglia hemorrhage (hematoma volume of 20-50 mL) were enrolled. The intercaudate dista...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22916-3
更新日期：2018-03-19 00:00:00
abstract：:There is emerging evidence that microRNAs (miRNAs) dysregulation is involved in the genesis and the progression of Prostate Cancer (PCa), thus potentially increasing their use in urological clinical practice. This is the first pilot study which utilizes Illumina Deep Sequencing to examine the entire miRNAs spectrum ex...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-24236-y
更新日期：2018-05-08 00:00:00
abstract：:The Japanese macaque (Macaca fuscata) exhibits a species-specific communication sound called the "coo call" to locate group members and maintain within-group contact. Monkeys have been demonstrated to be capable of discriminating between individuals based only on their voices, but there is still debate regarding how t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32042
更新日期：2016-08-23 00:00:00
abstract：:Limbal stem cell deficiency can be treated with transplantation of cultured human limbal epithelial cells (LEC). It can be advantageous to produce LEC in centralized labs and thereafter ship them to eye clinics. The present study used transport simulations of LEC to determine if vigorous shaking during transport alter...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28553-0
更新日期：2018-07-12 00:00:00
abstract：:The helicase Twinkle is indispensable for mtDNA replication in nucleoids. Previously, we showed that Twinkle is tightly membrane-associated even in the absence of mtDNA, which suggests that Twinkle is part of a membrane-attached replication platform. Here we show that this platform is a cholesterol-rich membrane struc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15292
更新日期：2015-10-19 00:00:00
abstract：:The L-iduronic acid (IdoA) residue is a critically important structural component in heparan sulphate polysaccharide for the biological functions. The pyranose ring of IdoA is present in (1)C4-chair, (2)SO-skew boat, and less frequently, in (4)C1-chair conformations. Here, we analyzed the conformation of IdoA residue ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29602
更新日期：2016-07-14 00:00:00
abstract：:White light generation by mixing red, green, and blue laser diodes (RGB LDs) was demonstrated with Commission International de l'Eclairage coordinates of (0.2928, 0.2981), a correlated color temperature of 8382 K, and a color rendering index of 54.4 to provide a maximal illuminance of 7540 lux. All the white lights ge...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00052-8
更新日期：2017-01-31 00:00:00
abstract：:Composite materials have become widely used in engineering applications, in order to reduce the overall weight of structures while retaining their required strength. In this work, a composite material consisting of discontinuous glass fibers in a polypropylene matrix is studied at the microstructural level through cou...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60368-w
更新日期：2020-02-27 00:00:00
abstract：:Animal gut harbors diverse microbes that play crucial roles in the nutrition uptake, metabolism, and the regulation of host immune responses. The intestinal microbiota homeostasis is critical for health but poorly understood. Probiotics Paracoccus marcusii DB11 and Bacillus cereus G19, and antibiotics florfenicol did ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05312-1
更新日期：2017-07-06 00:00:00
abstract：:Fear is an adaptive emotion that elicits defensive behavioural responses against aversive threats in animals. In mammals, serotonin receptors (5-HTRs) have been shown to modulate fear-related neural circuits in the basolateral amygdala complex (BLA). To understand the phylogenetic continuity of the neural basis for fe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78247-9
更新日期：2020-12-03 00:00:00
abstract：:The Synlestidae (Odonata: Zygoptera) of southern Africa comprise some highly localized species. All but one species are endemic to South Africa, and many to the Cape Floristic Region. Here we present the first phylogenetic reconstruction of the southern African Synlestidae using nuclear and mitochondrial molecular dat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72001-x
更新日期：2020-09-15 00:00:00
abstract：:A new miniplate applied differently from conventional application method for bone fixation has been developed. The novel approach is the insertion of the screw into the bone before miniplate installation. This study aimed to assess the stress distribution of a newly designed Yang's Keyhole (YK)- plate for segmental-bo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27186-7
更新日期：2018-06-12 00:00:00
