Abstract:
:In this study, we report a microfluidic device for the whole-life culture of the nematode Caenorhabditis elegans that allows the scoring of animal survival and health measures. This device referred to as the NemaLife chip features: (1) an optimized micropillar arena in which animals can crawl, (2) sieve channels that separate progeny and prevent the loss of adults from the arena during culture maintenance, and (3) ports that allow rapid accessibility for feeding the adult-only population and introducing reagents as needed. The pillar arena geometry was optimized to accommodate the growing body size during culture and emulate the body gait and locomotion of animals reared on agar. Likewise, feeding protocols were optimized to recapitulate longevity outcomes typical of standard plate growth. Key benefits of the NemaLife Chip include eliminating the need to perform repeated manual transfers of adults during survival assays, negating the need for progeny-blocking chemical interventions, and avoiding the swim-induced stress across lifespan in animals reared in liquid. We also show that the culture of animals in pillar-less microfluidic chambers reduces lifespan and introduces physiological stress by increasing the occurrence of age-related vulval integrity disorder. We validated our pillar-based device with longevity analyses of classical aging mutants (daf-2, age-1, eat-2, and daf-16) and animals subjected to RNAi knockdown of age-related genes (age-1 and daf-16). We also showed that healthspan measures such as pharyngeal pumping and tap-induced stimulated reversals can be scored across the lifespan in the NemaLife chip. Overall, the capacity to generate reliable lifespan and physiological data underscores the potential of the NemaLife chip to accelerate healthspan and lifespan investigations in C. elegans.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Rahman M,Edwards H,Birze N,Gabrilska R,Rumbaugh KP,Blawzdziewicz J,Szewczyk NJ,Driscoll M,Vanapalli SAdoi
10.1038/s41598-020-73002-6subject
Has Abstractpub_date
2020-10-01 00:00:00pages
16190issue
1issn
2045-2322pii
10.1038/s41598-020-73002-6journal_volume
10pub_type
杂志文章abstract::The relationship among the single nucleotide polymorphisms (SNPs) of the C-X-C motif chemokine ligand 12 gene (CXCL12) and the serum lipid profiles in the Chinese population has rarely been described, especially in somewhat old-fashioned and isolated Maonan minority. The goal of the current study was to elucidate the ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55725-3
更新日期:2019-12-20 00:00:00
abstract::Embryo implantation requires a hospitable uterine environment. A key metabolic change that occurs during the peri-implantation period, and throughout early pregnancy, is the rise in endometrial glycogen content. Glycogen accumulation requires prior cellular uptake of glucose. Here we show that both human and murine en...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11674-3
更新日期:2017-10-03 00:00:00
abstract::We study a minimal model of the stress-driven p53 regulatory network that includes competition between active and mutant forms of the tumor-suppressor gene p53. Depending on the nature and level of the external stress signal, four distinct dynamical states of p53 are observed. These states can be distinguished by diff...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-82054-1
更新日期:2021-01-27 00:00:00
abstract::A microfluidic probe (MFP) is a mobile channel-less microfluidic system under which a fluid is injected from an aperture into an open space, hydrodynamically confined by a surrounding fluid, and entirely re-aspirated into a second aperture. Various MFPs have been developed, and have been used for applications ranging ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11943
更新日期:2015-07-14 00:00:00
abstract::Tomato (Solanum Lycopersicum L.) is an important vegetable crop that belongs to the family Solanaceae. Root-knot nematodes reflect the highly critical economically damaging genera of phytoparasitic nematodes on tomato plants. In this study, the eco-nematicide activity of freshwater green macroalga Cladophora glomerata...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77005-1
更新日期:2020-11-17 00:00:00
abstract::Absolute numbers of lymphocytes are decreased in uninfected infants born to HIV-1-infected women (HIV-1-exposed). Although the exact mechanism is unknown, fetal exposure to maternal HIV-1-infection could prime the immune system and affect T cell trafficking. We compared the expression of chemokine receptors on cord bl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06690
更新日期:2014-10-24 00:00:00
abstract::Aerogels have numerous applications due to their high surface area and low densities. However, creating aerogels from a large variety of materials has remained an outstanding challenge. Here, we report a new methodology to enable aerogel production with a wide range of materials. The method is based on the assembly of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00849
更新日期:2012-01-01 00:00:00
abstract::Family studies have identified a heritable component to self-harm that is partially independent from comorbid psychiatric disorders. However, the genetic aetiology of broad sense (non-suicidal and suicidal) self-harm has not been characterised on the molecular level. In addition, controversy exists about the degree to...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66737-9
更新日期:2020-06-16 00:00:00
abstract::Enterovirus A71 (EV-A71) is an emerging pathogen in the Enterovirus A species group. EV-A71 causes hand, foot and mouth disease (HFMD), with virulent variants exhibiting polio-like acute flaccid paralysis and other central nervous system manifestations. We analysed all enterovirus A71 complete genomes with collection ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41662-8
更新日期:2019-04-01 00:00:00
abstract::Patients with advanced ovarian cancer develop recurrence despite initial treatment response to standard treatment of surgery and intravenous/intraperitoneal (IP) chemotherapy, partly due to a limited peritoneal exposure time of chemotherapeutics. Paclitaxel-loaded genipin-crosslinked gelatin microspheres (PTX-GP-MS) a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51419-y
更新日期:2019-10-16 00:00:00
abstract::We construct two families of orthogonal product quantum states that cannot be exactly distinguished by local operation and classical communication (LOCC) in the quantum system of (2k+i) ⊗ (2l+j) (i, j ∈ {0, 1} and i ≥ j ) and (3k+i) ⊗ (3l+j) (i, j ∈ {0, 1, 2}). And we also give the tiling structure of these two famili...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28864
更新日期:2016-07-05 00:00:00
abstract::Rapid changes observed today in mountain glaciers need to be put into a longer-term context to understand global sea-level contributions, regional climate-glacier systems and local landscape evolution. In this study we determined volume changes for 400 mountain glaciers across the Southern Alps, New Zealand for three ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-70276-8
更新日期:2020-08-07 00:00:00
abstract::Molecular imaging technique is an attractive tool to detect liver disease at early stage. This study aims to develop a simultaneous dual-isotope single photon emission computed tomography (SPECT)/CT imaging method to assist diagnosis of hepatic tumor and liver fibrosis. Animal models of liver fibrosis and orthotopic h...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28812
更新日期:2016-07-05 00:00:00
abstract::Dementia is a major public health issue worldwide and chronic use of benzodiazepine, which is very frequent in northern countries, was found to be a risk factor of dementia. This work aims at evaluating the impact of a reduction in chronic use of benzodiazepine on the future burden of dementia in France. Using estimat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71482-0
更新日期:2020-09-04 00:00:00
abstract::The gut microbiota (GM) is related to obesity and other metabolic diseases. To detect GM markers for obesity in patients with different metabolic abnormalities and investigate their relationships with clinical indicators, 1,914 Chinese adults were enrolled for 16S rRNA gene sequencing in this retrospective study. Base...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/s41598-019-49462-w
更新日期:2019-09-17 00:00:00
abstract::The myxozoan parasite, Tetracapsuloides bryosalmonae has a two-host life cycle alternating between freshwater bryozoans and salmonid fish. Infected fish can develop Proliferative Kidney Disease, characterised by a gross lymphoid-driven kidney pathology in wild and farmed salmonids. To facilitate an in-depth understand...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77881-7
更新日期:2021-01-25 00:00:00
abstract::Transmembrane-4-L-six-family-1(TM4SF1), a four-transmembrane L6 family member, is highly expressed in various pancreatic cancer cell lines and promotes cancer cells metastasis. However, the TM4SF1-associated signaling network in metastasis remains unknown. In the present study, we found that TM4SF1 affected the format...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45895
更新日期:2017-04-03 00:00:00
abstract::In primary Sjögren's syndrome (pSS) the exocrine glands become infiltrated with lymphocytes instigating severe damage to the salivary and lacrimal glands causing dry eyes and dry mouth. Previous investigations have suggested that dysregulated localized and systemic inflammation contributes to the development and patho...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-020-64422-5
更新日期:2020-05-04 00:00:00
abstract::In order to elucidate features of the denatured state ensembles that exist in equilibrium with the native state under physiological conditions, we performed 1.4-μs molecular dynamics (MD) simulations at 400 K and 450 K using the monomer subunits of three CutA1 mutants from Escherichia coli: an SH-free mutant (Ec0SH) w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-25825-7
更新日期:2018-05-16 00:00:00
abstract::Neospora caninum is an apicomplexan parasite responsible for major economic losses due to abortions in cattle. Innate immune responses are crucial for host resistance against the infection, however the molecules involved in parasite recognition are still poorly understood. Nod2 is a cytosolic receptor that recognizes ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29289
更新日期:2016-07-05 00:00:00
abstract::Use of human induced pluripotent stem cells (h-iPSCs) for bone tissue engineering is most appealing, because h-iPSCs are an inexhaustible source of osteocompetent cells. The present study investigated the contribution of undifferentiated h-iPSCs and elucidated aspects of the underlying mechanism(s) of the involvement ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35546-6
更新日期:2018-11-20 00:00:00
abstract::Radiation exposure severely damages the hematopoietic system. Although several radio-protectors have been proposed to prevent radiation-induced damage, most agents have limited efficacy. In the present study, we investigated whether mesenchymal stem cells (MSCs) could contribute to the expansion of hematopoietic cells...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27666-w
更新日期:2018-06-18 00:00:00
abstract::The causes of the severest crisis in the history of life around the Permian-Triassic boundary (PTB) remain controversial. Here we report that the latest Permian alluvial plains in Shanxi, North China, went through a rapid transition from meandering rivers to braided rivers and aeolian systems. Soil carbonate carbon is...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53321-z
更新日期:2019-11-14 00:00:00
abstract::Over the history of carbon, it is generally acknowledged that Bernal AB stacking of the sp2 carbon layers is the unique crystalline form of graphite. The universal graphite structure is synthesized at 2,600~3,000 °C and exhibits a micro-polycrystalline feature. In this paper, we provide evidence for a metastable form ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39624
更新日期:2016-12-21 00:00:00
abstract::An encapsulated formulation of Bacillus thuringiensis (Bt) was produced by the Pickering emulsion technique to improve its activity and stability under UV-A radiation. In this technique latex particles, GO nanosheets, olive oil, ethanol, and water were used to encapsulate Bt in colloidosomes. The protective efficacy o...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77721-8
更新日期:2020-11-26 00:00:00
abstract::This study examines the analytical and numerical solution of electromagnetic surface waves supported by a resistive metasurface-covered grounded metamaterial structure. To simulate the metamaterial, the Kramers-Kronig relation based on the causality principle is used, while the modeling of the resistive metasurface ha...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72396-7
更新日期:2020-09-23 00:00:00
abstract::Insecticides and other agrochemicals have become indispensable components of the agricultural system to ensure a notable increase in crop yield and food production. As a natural consequence, chemical residues result in significantly increased contamination of both terrestrial and aquatic ecosystems. The present study ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76638-6
更新日期:2020-11-11 00:00:00
abstract::Morphometric correlation networks of cortical thickness, surface area, and gray matter volume have statistically different structural topology. However, there is no report directly describing their correlation patterns in view of interregional covariance. Here, we examined the characteristics of the correlation patter...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26682
更新日期:2016-05-26 00:00:00
abstract::Histological molecular classification of hepatocellular carcinoma (HCC) is clinically important for predicting the prognosis. However, a reliable serum marker has not been established. The aim of this study was to evaluate the diagnostic value of serum Wisteria Floribunda agglutinin-positive sialylated mucin 1 (WFA-si...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00357-8
更新日期:2017-03-21 00:00:00
abstract::Episodic memories are long lasting and full of detail, yet imperfect and malleable. We quantitatively evaluated recollection of short audiovisual segments from movies as a proxy to real-life memory formation in 161 subjects at 15 minutes up to a year after encoding. Memories were reproducible within and across individ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30175
更新日期:2016-09-30 00:00:00