Abstract：
:The cell cytosol is crowded with high concentrations of many different biomacromolecules, which is difficult to mimic in bottom-up synthetic cell research and limits the functionality of existing protocellular platforms. There is thus a clear need for a general, biocompatible, and accessible tool to more accurately emulate this environment. Herein, we describe the development of a discrete, membrane-bound coacervate-based protocellular platform that utilizes the well-known binding motif between Ni2+-nitrilotriacetic acid and His-tagged proteins to exercise a high level of control over the loading of biologically relevant macromolecules. This platform can accrete proteins in a controlled, efficient, and benign manner, culminating in the enhancement of an encapsulated two-enzyme cascade and protease-mediated cargo secretion, highlighting the potency of this methodology. This versatile approach for programmed spatial organization of biologically relevant proteins expands the protocellular toolbox, and paves the way for the development of the next generation of complex yet well-regulated synthetic cells.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Altenburg WJ,Yewdall NA,Vervoort DFM,van Stevendaal MHME,Mason AF,van Hest JCMdoi
10.1038/s41467-020-20124-0subject
Has Abstractpub_date
2020-12-08 00:00:00pages
6282issue
1issn
2041-1723pii
10.1038/s41467-020-20124-0journal_volume
11pub_type
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