Mysterious and fascinating: DNA polymerase ɩ remains enigmatic 20 years after its discovery.

abstract：:Genome integrity is constantly challenged by exogenous and endogenous insults, and mutations are associated with inherited disease and cancer. Here we summarize recent studies that utilized C. elegans whole genome next generation sequencing to experimentally determine mutational signatures associated with mutagen expo...

journal_title：DNA repair

authors： Meier B,Volkova NV,Gerstung M,Gartner A

更新日期：2020-11-01 00:00:00

abstract：:DNA damage created by endogenous or exogenous genotoxic agents can exist in multiple forms, and if allowed to persist, can promote genome instability and directly lead to various human diseases, particularly cancer, neurological abnormalities, immunodeficiency and premature aging. To avoid such deleterious outcomes, c...

journal_title：DNA repair

authors： Iyama T,Wilson DM 3rd

更新日期：2013-08-01 00:00:00

abstract：:Bacterial RarA is thought to play crucial roles in the cellular response to blocked replication forks. We show that lack of Bacillus subtilis RarA renders cells very sensitive to H2O2, but not to methyl methane sulfonate or 4-nitroquinoline-1-oxide. RarA is epistatic to RecA in response to DNA damage. Inactivation of ...

journal_title：DNA repair

authors： Romero H,Torres R,Hernández-Tamayo R,Carrasco B,Ayora S,Graumann PL,Alonso JC

更新日期：2019-06-01 00:00:00

abstract：:Both UVB radiation and DNA-breaking agents were previously reported to kill Arabidopsis stem cells. We demonstrate that death induced by UVB or by ionizing radiation (IR) requires Suppressor of Gamma Response 1 (SOG1), a transcription factor already found to govern many responses to these agents in Arabidopsis. DNA-da...

journal_title：DNA repair

authors： Furukawa T,Curtis MJ,Tominey CM,Duong YH,Wilcox BW,Aggoune D,Hays JB,Britt AB

更新日期：2010-09-04 00:00:00

abstract：:Before a deleterious DNA lesion can be replaced with its undamaged counterpart, the lesion must first be removed from the genome. This process of removing and replacing DNA lesions is accomplished by the careful coordination of several protein factors during DNA repair. One such factor is the multifunctional enzyme hu...

journal_title：DNA repair

authors： Whitaker AM,Freudenthal BD

更新日期：2018-11-01 00:00:00

abstract：:Methylation of the O(6)-methylguanine-DNA-methyltransferase (MGMT) promoter is associated with G:C to A:T transitions in the p53 gene in various human cancers, including lung cancer. In tumors with p53 mutation, MGMT promoter methylation is more common in advanced tumors than in early tumors. However, in tumors with w...

journal_title：DNA repair

authors： Lai JC,Cheng YW,Goan YG,Chang JT,Wu TC,Chen CY,Lee H

更新日期：2008-08-02 00:00:00

abstract：:Loss of telomeres stability is a hallmark of cancer cells. Exposed telomeres are prone to aberrant end-joining reactions leading to chromosomal fusions and translocations. Human telomeres contain repeated TTAGGG elements, in which the 3' exposed strand may adopt a G-quadruplex (G4) structure. The guanine-rich regions ...

journal_title：DNA repair

authors： Burra S,Marasco D,Malfatti MC,Antoniali G,Virgilio A,Esposito V,Demple B,Galeone A,Tell G

更新日期：2019-01-01 00:00:00

abstract：:Conflicts between replication and transcription can have life-threatening consequences. RNA polymerase (RNAP) is the major impediment to replication progression, and its efficient removal from DNA should mitigate the consequences of collisions with replication. Cells have various proteins that can resolve conflicts by...

journal_title：DNA repair

authors： Ragheb M,Merrikh H

更新日期：2019-09-01 00:00:00

abstract：:During DNA synthesis in vitro using dNTP and rNTP concentrations present in vivo, yeast replicative DNA polymerases α, δ and ɛ (Pols α, δ and ɛ) stably incorporate rNTPs into DNA. rNTPs are also incorporated during replication in vivo, and they are repaired in an RNase H2-dependent manner. In strains encoding a mutato...

journal_title：DNA repair

authors： Clark AB,Lujan SA,Kissling GE,Kunkel TA

更新日期：2011-05-05 00:00:00

abstract：:The linear plasmid (pPac1-2) encoded killer toxin (PaT) of the yeast Pichia acaciae arrests sensitive Saccharomyces cerevisiae cells in the S-phase of the cell cycle and induces mutations. Here we provide evidence for opposite effects in PaT resistance of homologous recombination (HR) and non-homologous end joining (N...

journal_title：DNA repair

authors： Klassen R,Krampe S,Meinhardt F

更新日期：2007-12-01 00:00:00

abstract：:Nucleotide excision repair and reversal of pyrimidine dimers by photolyase (photoreactivation) are two major pathways to remove UV-lesions from DNA. Here, it is discussed how lesions are recognized and removed when the DNA is condensed into nucleosomes. During the recent years it was shown that nucleosomes inhibit pho...

journal_title：DNA repair

authors： Thoma F

更新日期：2005-07-28 00:00:00

abstract：:We studied how DNA divergence between recombining DNAs and the mismatch repair system modulate the SOS response in Escherichia coli. The observed positive log-linear correlation between SOS induction and DNA divergence, and the negative correlation between SOS induction and frequency of recombination, suggest that the...

journal_title：DNA repair

authors： Delmas S,Matic I

更新日期：2005-02-03 00:00:00

abstract：:Oxidative stress via redox reactions can regulate DNA repair pathways. The base excision repair (BER) enzyme apurinic/apyrimidinic endonuclease 1 (APE1) is a key player in the redox regulation of DNA repair. Environmental factors can alter the methylation of DNA repair genes, change their expression and thus modulate ...

journal_title：DNA repair

authors： Langie SA,Kowalczyk P,Tomaszewski B,Vasilaki A,Maas LM,Moonen EJ,Palagani A,Godschalk RW,Tudek B,van Schooten FJ,Berghe WV,Zabielski R,Mathers JC

更新日期：2014-06-01 00:00:00

abstract：:Ionizing radiation induces clustered DNA damaged sites, defined as two or more lesions formed within one or two helical turns of the DNA through passage of a single radiation track. It is now established that clustered DNA damage sites are found in cells and present a challenge to the repair machinery of the cell but ...

journal_title：DNA repair

authors： Cunniffe SM,Lomax ME,O'Neill P

更新日期：2007-12-01 00:00:00

abstract：:The First joint meeting of the German DGDR (German Society for Research on DNA Repair) and the French SFTG (French Society of Genotoxicology) on DNA Repair was held in Toulouse, France, from September 15 to 19, 2007. It was organized by Lisa Wiesmüller and Bernard Salles together with the scientific committee consisti...

journal_title：DNA repair

authors： Lavelle C,Salles B,Wiesmüller L

更新日期：2008-04-02 00:00:00

abstract：:Mammalian cells have evolved sophisticated DNA repair systems to correct mispaired or damaged bases and extrahelical loops. Emerging evidence suggests that, in some cases, the normal DNA repair machinery is "hijacked" to become a causative factor in mutation and disease, rather than act as a safeguard of genomic integ...

journal_title：DNA repair

authors： McMurray CT

更新日期：2008-07-01 00:00:00

abstract：:In both Schizosaccharomyces pombe and Saccharomyces cerevisiae, Mms22 and Mms1 form a complex with important functions in the response to DNA damage, loss of which leads to perturbations during replication. Furthermore, in S. cerevisiae, Mms1 has been suggested to function in concert with a Cullin-like protein, Rtt101...

journal_title：DNA repair

authors： Vejrup-Hansen R,Mizuno K,Miyabe I,Fleck O,Holmberg C,Murray JM,Carr AM,Nielsen O

更新日期：2011-03-07 00:00:00

abstract：:The Tousled-like kinases are involved in chromatin assembly, DNA repair, transcription, and chromosome segregation. In this work, we show that overexpression of TLK1B hastens repair of double strand breaks (DSBs) in mouse cells. We have identified Rad9 as a protein interacting tightly with TLK1B. TLK1B phosphorylates ...

journal_title：DNA repair

authors： Sunavala-Dossabhoy G,De Benedetti A

更新日期：2009-01-01 00:00:00

abstract：:Quantitating relative (32)P-band intensity in gels is desired, e.g., to study primer-extension kinetics of DNA polymerases (DNAPs). Following imaging, multiple (32)P-bands are often present in lanes. Though individual bands appear by eye to be simple and well-resolved, scanning reveals they are actually skewed-Gaussia...

journal_title：DNA repair

authors： Sholder G,Loechler EL

更新日期：2015-01-01 00:00:00

abstract：:Reactive oxygen species (ROS) are formed as natural byproducts during aerobic metabolism and readily induce premutagenic base lesions in the DNA. The 8-oxoguanine DNA glycosylase (OGG1) and MutY homolog 1 (MYH) synergistically prevent mutagenesis and cancer formation in mice. Their localization in the mitochondria as ...

journal_title：DNA repair

authors： Halsne R,Esbensen Y,Wang W,Scheffler K,Suganthan R,Bjørås M,Eide L

更新日期：2012-03-01 00:00:00

abstract：:The Werner syndrome (WS) protein (WRN), a DNA helicase/exonuclease, is required for genomic stability and avoidance of cancer. Current evidence suggests that WRN is involved in the resolution of stalled and/or collapsed replication forks. This function is indicated, in part, by replication defects in WS cells and by h...

journal_title：DNA repair

authors： Blank A,Bobola MS,Gold B,Varadarajan S,D Kolstoe D,Meade EH,Rabinovitch PS,Loeb LA,Silber JR

更新日期：2004-06-03 00:00:00

abstract：:There is an increasing demand for phenotyping assays in the field of human functional genetics. DNA repair activity is representative of this functional approach, being seen as a valuable biomarker related to cancer risk. Repair activity is evaluated by incubating a cell extract with a DNA substrate containing lesions...

journal_title：DNA repair

authors： Azqueta A,Langie SA,Slyskova J,Collins AR

更新日期：2013-11-01 00:00:00

abstract：:Nucleotide excision repair (NER) is the principal pathway for the removal of a wide range of DNA helix-distorting lesions. Two NER subpathways have been identified, i.e. global genome repair (GGR) and transcription-coupled repair (TCR). Little is known about the expression of NER pathways in differentiated cells. We a...

journal_title：DNA repair

authors： van der Wees CG,Vreeswijk MP,Persoon M,van der Laarse A,van Zeeland AA,Mullenders LH

更新日期：2003-12-09 00:00:00

abstract：:All organisms rely on integrated networks to repair DNA double-strand breaks (DSBs) in order to preserve the integrity of the genetic information, to re-establish replication, and to ensure proper chromosomal segregation. Genetic, cytological, biochemical and structural approaches have been used to analyze how Bacillu...

journal_title：DNA repair

authors： Alonso JC,Cardenas PP,Sanchez H,Hejna J,Suzuki Y,Takeyasu K

更新日期：2013-03-01 00:00:00

abstract：:The continuity of duplex DNA is generally considered a prerequisite for chromosome continuity. However, as previously shown in yeast as well as human cells, the introduction of a double-strand break (DSB) does not generate a chromosome break (CRB) in yeast or human cells. The transition from DSB to CRB was found to be...

journal_title：DNA repair

authors： Nakai W,Westmoreland J,Yeh E,Bloom K,Resnick MA

更新日期：2011-01-02 00:00:00

abstract：:The Escherichia coli orf135 gene encodes a 15.4kDa protein with homology to the MutT family of nucleotide hydrolases. The orf135 gene was cloned within a glutathione S-transferase (GST) fusion protein expression vector, which was used to overproduce the GST-Orf135 fusion protein in E. coli. The fusion protein thus obt...

journal_title：DNA repair

authors： Fujikawa K,Kasai H

更新日期：2002-07-17 00:00:00

abstract：:From bacteria to humans, ancient stress responses enable organisms to contend with damage to both the genome and the proteome. These pathways have long been viewed as fundamentally separate responses. Yet recent discoveries from multiple fields have revealed surprising links between the two. Many DNA-damaging agents a...

journal_title：DNA repair

authors： Xie JL,Jarosz DF

更新日期：2018-11-01 00:00:00

abstract：:Regulation of the vertebrate checkpoint kinase Chk1 involves several protein complexes including the recently identified protein Claspin. Claspin associates with Chk1 upon replication stress and DNA damage and is required for Chk1 activation in both Xenopus and human systems. More importantly, Claspin is involved in r...

journal_title：DNA repair

authors： Chini CC,Chen J

更新日期：2004-08-01 00:00:00

abstract：:Cancer-prone diseases ataxia-telangiectasia (AT), Nijmegen breakage syndrome (NBS) and ataxia-telangiectasia-like disorder (ATLD) are defective in the repair of DNA double-stranded break (DSB). On the other hand, arsenic (As) has been reported to cause DSB and to be involved in the occurrence of skin, lung and bladder...

journal_title：DNA repair

authors： Yuan SS,Su JH,Hou MF,Yang FW,Zhao S,Lee EY

更新日期：2002-02-28 00:00:00

abstract：:The repair of DNA double strand breaks is essential for cell survival and several conserved pathways have evolved to ensure their rapid and efficient repair. The non-homologous end joining pathway is initiated when Ku binds to the DNA break site. Ku is an abundant nuclear heterodimer of Ku70 and Ku80 with a toroidal s...

journal_title：DNA repair

authors： Grundy GJ,Moulding HA,Caldecott KW,Rulten SL

更新日期：2014-05-01 00:00:00