Abstract：
:Conflicts between replication and transcription can have life-threatening consequences. RNA polymerase (RNAP) is the major impediment to replication progression, and its efficient removal from DNA should mitigate the consequences of collisions with replication. Cells have various proteins that can resolve conflicts by removing stalled (or actively translocating) RNAP from DNA. It would therefore seem logical that RNAP-associated factors, such as the bacterial DNA translocase Mfd, would minimize the effects of conflicts. Despite seemingly conclusive statements in most textbooks, the role of Mfd in conflicts remains an enigma. In this review, we will discuss the different physical states of RNAP during transcription, and how each distinct state can influence conflict severity and potentially trigger the involvement of Mfd. We propose models to explain the contradictory conclusions from published studies on the potential role of Mfd in resolving conflicts.
journal_name
DNA Repair (Amst)journal_title
DNA repairauthors
Ragheb M,Merrikh Hdoi
10.1016/j.dnarep.2019.102659subject
Has Abstractpub_date
2019-09-01 00:00:00pages
102659eissn
1568-7864issn
1568-7856pii
S1568-7864(19)30212-5journal_volume
81pub_type
杂志文章,评审相关文献
DNA REPAIR文献大全abstract：:PriA, a 3'-->5' superfamily 2 DNA helicase, acts to remodel stalled replication forks and as a specificity factor for origin-independent assembly of a new replisome at the stalled fork. The ability of PriA to initiate replication at stalled forked structures ensures complete genome replication and helps to protect the...
journal_title：DNA repair
pub_type： 杂志文章,评审
doi：10.1016/j.dnarep.2009.12.009
更新日期：2010-03-02 00:00:00
abstract：:DNA-PKcs deficiency has been studied in numerous animal models and cell culture systems. In previous studies of kinase inactivating mutations in cell culture systems, ablation of DNA-PK's catalytic activity results in a cell phenotype that is virtually indistinguishable from that ascribed to complete loss of the enzym...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2018.10.004
更新日期：2019-01-01 00:00:00
abstract：:Huntington disease (HD) is associated with an unstable trinucleotide CAG.CTG repeat expansion. Although the repeat length is inversely correlated with the age-at-onset of symptoms, variability between patients who have inherited the same HD repeat length clearly suggests that other factors influence this aspect of the...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2007.01.002
更新日期：2007-06-01 00:00:00
abstract：:In all organisms studied to date, 8-oxoguanine (GO), an important oxidation product of guanine, is removed by highly conserved GO DNA glycosylases. The hyperthermophilic crenarchaeon Pyrobaculum aerophilum encodes a GO DNA glycosylase, Pa-AGOG (Archaeal GO DNA glycosylase) which has become the founding member of a new...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2009.03.009
更新日期：2009-07-04 00:00:00
abstract：:The human RAD51 recombinase possesses DNA pairing and strand exchange activities that are essential for the error-free, homology-directed repair of DNA double-strand breaks. The recombination activities of RAD51 are activated upon its assembly into presynaptic filaments on single-stranded DNA at resected DSB ends. Def...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2017.10.008
更新日期：2017-12-01 00:00:00
abstract：:Gene amplification, a key mechanism for oncogene activation and drug resistance in tumour cells, involves the generation and joining of DNA double-strand breaks. Amplified DNA can be carried either on intra-chromosomal arrays or on extra-chromosomal elements (double minutes). We previously showed that, in rodent cells...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2008.08.015
更新日期：2009-01-01 00:00:00
abstract：:The proposed mechanism for transcription coupled nucleotide excision repair (TCR) invokes RNA polymerase (RNAP) blocked at a DNA lesion as a signal to initiate repair. In Escherichia coli, TCR requires the interaction of RNAP with a transcription-repair coupling factor encoded by the mfd gene. The interaction between ...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2007.04.002
更新日期：2007-10-01 00:00:00
abstract：:The Tousled-like kinases are involved in chromatin assembly, DNA repair, transcription, and chromosome segregation. In this work, we show that overexpression of TLK1B hastens repair of double strand breaks (DSBs) in mouse cells. We have identified Rad9 as a protein interacting tightly with TLK1B. TLK1B phosphorylates ...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2008.09.005
更新日期：2009-01-01 00:00:00
abstract：:Nijmegen breakage syndrome is a recessive genetic disorder, characterized by elevated sensitivity to ionizing radiation, chromosome instability and high frequency of malignancies. Since cellular features partly overlap with those of ataxia-telangiectasia (A-T), NBS was long considered an A-T clinical variant. NBS1, th...
journal_title：DNA repair
pub_type： 杂志文章,评审
doi：10.1016/j.dnarep.2004.03.023
更新日期：2004-08-01 00:00:00
abstract：:Xeroderma pigmentosum (XP) is a genetic disorder associated with defects in nucleotide excision repair, a pathway that eliminates a wide variety of helix-distorting DNA lesions, including ultraviolet-induced pyrimidine dimers. In addition to skin diseases in sun-exposed areas, approximately 25% of XP patients develop ...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2019.04.004
更新日期：2019-08-01 00:00:00
abstract：:Before a deleterious DNA lesion can be replaced with its undamaged counterpart, the lesion must first be removed from the genome. This process of removing and replacing DNA lesions is accomplished by the careful coordination of several protein factors during DNA repair. One such factor is the multifunctional enzyme hu...
journal_title：DNA repair
pub_type： 杂志文章,评审
doi：10.1016/j.dnarep.2018.08.012
更新日期：2018-11-01 00:00:00
abstract：:The ubiquitin-proteasome pathway plays an important role in DNA damage signaling and repair by facilitating the recruitment and activation of DNA repair factors and signaling proteins at sites of damaged chromatin. Proteasome activity is generally not thought to be required for activation of apical signaling kinases i...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2009.10.008
更新日期：2010-01-02 00:00:00
abstract：:DNA mismatch repair is an evolutionarily conserved repair pathway that corrects replication errors. In most prokaryotes and all eukaryotes, the mismatch repair protein MutL is a sequence-unspecific endonuclease that nicks the newly synthesized strand and marks it for repair. Although the sequence of the endonuclease d...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2018.10.003
更新日期：2019-01-01 00:00:00
abstract：:The enzyme activation-induced deaminase (AID) targets the immunoglobulin loci in activated B cells and creates DNA mutations in the antigen-binding variable region and DNA breaks in the switch region through processes known, respectively, as somatic hypermutation and class switch recombination. AID deaminates cytosine...
journal_title：DNA repair
pub_type： 杂志文章,评审
doi：10.1016/j.dnarep.2015.11.011
更新日期：2016-02-01 00:00:00
abstract：:Accumulation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG) in DNA is associated with mutagenesis and cell death. Little attention has been given to the biological significance of 8-oxo-dG accumulation in cardiovascular tissues during the different stage of hypertension and its prevention. We thus investigated the ...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2007.01.003
更新日期：2007-06-01 00:00:00
abstract：:Double-strand breaks in genomic DNA (DSB) are potentially lethal lesions which separate parts of chromosome arms from their centromeres. Repair of DSB by recombination can generate mutations and further chromosomal rearrangements, making the regulation of recombination and the choice of recombination pathways of the h...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2011.04.002
更新日期：2011-06-10 00:00:00
abstract：:Deinococcus radiodurans recovering from the effect of acute dose of gamma (gamma) radiation shows a biphasic mechanism of DNA double strands breaks repair that involves an efficient homologous recombination. However, it shows higher sensitivity to near-UV (NUV) than Escherichia coli and lacks RecBC, a DNA strand break...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2007.07.007
更新日期：2008-01-01 00:00:00
abstract：:SbcCD and other Mre11/Rad50 (MR) complexes are implicated in the metabolism of DNA ends. They cleave ends sealed by hairpin structures and have been postulated to play roles in removing protein bound to DNA termini. Here we provide direct evidence that the Escherichia coli MR complex (SbcCD) removes protein from a pro...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/s1568-7864(03)00063-6
更新日期：2003-07-16 00:00:00
abstract：:The mechanism and determinants of RecA mediated initial alignment of homologous DNA molecules were studied by performing Monte Carlo simulations of the dynamics of DNA molecules. The simulation procedure was used to assess the effect of heterologous DNA and dilution on the rate of formation and yield of homologous ali...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2003.09.009
更新日期：2004-01-05 00:00:00
abstract：:AA8 Chinese hamster ovary cells were treated with halogenated nucleosides analogues of thymidine, namely CldU, 5-iodo-2'-deoxyuridine (IdU), and 5-bromo-2'-deoxyuridine (BrdU), following different experimental protocols. The purpose was to see whether incorporation of exogenous pyrimidine analogues into DNA could inte...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/s1568-7864(03)00044-2
更新日期：2003-06-11 00:00:00
abstract：:Cockayne syndrome (CS) is a debilitating and complex disorder that results from inherited mutations in the CS complementation genes A and B, CSA and CSB. The links between the molecular functions of the CS genes and the complex pathophysiology of CS are as of yet poorly understood and are the subject of intense debate...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2014.09.011
更新日期：2014-12-01 00:00:00
abstract：:Exposure to ionizing radiation results in a variety of genome rearrangements that have been linked to tumor formation. Many of these rearrangements are thought to arise from the repair of double-strand breaks (DSBs) by several mechanisms, including homologous recombination (HR) between repetitive sequences dispersed t...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2008.02.003
更新日期：2008-05-03 00:00:00
abstract：:RAre DAmage and Repair sequencing (RADAR-seq) is a highly adaptable sequencing method that enables the identification and detection of rare DNA damage events for a wide variety of DNA lesions at single-molecule resolution on a genome-wide scale. In RADAR-seq, DNA lesions are replaced with a patch of modified bases tha...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2019.06.007
更新日期：2019-08-01 00:00:00
abstract：:Nucleotide excision repair (NER) is a conserved DNA repair mechanism capable of removing a variety of helix-distorting DNA lesions. Rad26, a member of the Swi2/Snf2 superfamily of proteins, has been shown to be involved in a specialized NER process called transcription coupled NER. Rad16, another member of the same pr...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2007.05.005
更新日期：2007-11-01 00:00:00
abstract：:Endonuclease III (EndoIII) is nearly ubiquitous in all three domains of life. EndoIII family proteins exhibit a bifunctional (glycosylase/lyase) activity on oxidative/saturated pyrimidine bases, such as thymine glycol. Previous studies on EndoIII homologs have reported the presence of important residues involved in su...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2020.102859
更新日期：2020-06-01 00:00:00
abstract：:In eukaryotes, mutations in a number of genes that affect DNA damage checkpoints or DNA replication also affect telomere length [Curr. Opin. Cell Biol. 13 (2001) 281]. Saccharomyces cerevisae strains with mutations in the TEL1 gene (encoding an ATM-like protein kinase) have very short telomeres, as do strains with mut...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/s1568-7864(03)00115-0
更新日期：2003-09-18 00:00:00
abstract：:Human cancers frequently harbour mutations in DNA repair genes, rendering the use of DNA damaging agents as an effective therapeutic intervention. As therapy-resistant cells often arise, it is important to better understand the molecular pathways that drive resistance in order to facilitate the eventual targeting of s...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2020.102939
更新日期：2020-11-01 00:00:00
abstract：:Mammalian cells possess multiple closely related SWI/SNF chromatin remodelling complexes. These complexes have been implicated in the cellular response to DNA double strand breaks (DSBs). Evidence suggests that SWI/SNF complexes contribute to successful repair via both the homologous recombination and non-homologous e...
journal_title：DNA repair
pub_type： 杂志文章,评审
doi：10.1016/j.dnarep.2020.102919
更新日期：2020-09-01 00:00:00
abstract：:RecQ5 belongs to the RecQ DNA helicase family that includes genes causative of Bloom, Werner, and Rothmund-Thomson syndromes. Although no human disease has been genetically linked to a mutation in RecQ5, Drosophila melanogaster RecQ5 is highly expressed in early embryos, suggesting an important role for it in the DNA ...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2008.10.007
更新日期：2009-02-01 00:00:00
abstract：:recX is a small open reading frame located downstream of recA that is conserved in many bacteria. In Escherichia coli, the recX gene (also named oraA) is a 501 bp open reading frame that encodes a predicted basic protein. Transcriptional analysis by Northern blots showed that in E. coli the recX gene is SOS-regulated....
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/s1568-7864(02)00217-3
更新日期：2003-03-01 00:00:00
