Abstract：
:The effects of autophagy on neuronal damage can be positive or detrimental negative. Through establishing a model of fetal rat cortical neuron hydraulic shock injury, dipotassium bisperoxo (picolinoto) oxovanadate (V) [bpv(pic)] was used to inhibit PTEN at different time points post-injury and autophagy level after neuronal injury was assessed. Neurons were divided into several intervention groups according to the time point at which bpv(pic) was used to inhibit autophagy, normal neurons and injuried neurons were set as two control groups. Growth of neurons in each group was assessed through immunofluorescence staining. Expression of the autophagy-related proteins LC3-II and LC3-I was analyzed by western blot. Expression of PTEN, mTOR and Beclin-1 was detected by RT-PCR. The number of autophagosomes in the normal group, injury control group and 24 h, 36 h intervention groups were assessed by electron microscope. We found that autophagy was enhanced after neuronal injury and that the levels of LC3-II was significantly reduced by bpv (pic) intervention. The growth of the injury control groups was worse than normal groups, while improved through bpv(pic) intervention at 24 h and 30 h after injured. Western blot analysis showed that the LC3-II and LC3-II/LC3-I ratios of cells increased post-injury, and autophagy induction was evident by electron microscopy. These effects were confirmed by RT-PCR analysis. Taken together, these data suggest that autophagy is activated after injury in neurons while can be inhibited by bpv(pic) administration and then promote the repair of injured neurons.
journal_name
Brain Res Bulljournal_title
Brain research bulletinauthors
Yu H,Shao J,Huang R,Guan Y,Li G,Chen S,Zhou F,Yao Q,Shen Jdoi
10.1016/j.brainresbull.2020.10.008subject
Has Abstractpub_date
2020-12-01 00:00:00pages
161-168eissn
0361-9230issn
1873-2747pii
S0361-9230(20)30665-1journal_volume
165pub_type
杂志文章abstract：:An animal with an unimpaired "reference" memory can distinguish between alternatives that belong to a rewarded set and those that are unrewarded. An animal with an unimpaired "working" memory can distinguish between alternatives where it has been rewarded (e.g., food has been eaten and not replaced) and those where it...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(81)90086-1
更新日期：1981-08-01 00:00:00
abstract：:The neuropathology of Alzheimer disease is characterized by senile plaques, neurofibrillary tangles and cell death. These hallmarks develop according to the differential vulnerability of brain networks, senile plaques accumulating preferentially in the associative cortical areas and neurofibrillary tangles in the ento...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/j.brainresbull.2009.06.006
更新日期：2009-10-28 00:00:00
abstract：:It is known that spinal nociceptive sensory transmission receives descending inhibitory and facilitatory modulation from supraspinal structures. Glutamate is the major fast excitatory transmitter between primary afferent fibers and spinal dorsal horn neurons. In whole-cell patch clamp recordings from dorsal horn neuro...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(01)00470-1
更新日期：2001-04-01 00:00:00
abstract：:In eleven acute precollicular rats, cortical and hippocampal EEG activity was recorded. Hippocampal theta activity of low frequency (mean 4.5 c/sec) was found in nine rats and in four occupied above 50% of the recording time. In contrast, in the cortex the synchronized EEG activity dominated. The electrical stimulatio...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(89)90006-3
更新日期：1989-06-01 00:00:00
abstract：:The relationship between the "passions" (emotions or feelings) and psychopathology has been a constant throughout the history of medicine. In this context, melancholy was considered a perversion of the soul (corruption of the passions). One of the most influential authors on this subject was René Descartes, who discus...
journal_title：Brain research bulletin
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.brainresbull.2011.01.018
更新日期：2011-04-25 00:00:00
abstract：:How deep brain stimulation (DBS) acts and how the brain responds to it remains unclear. To investigate the mechanisms involved, we analyzed changes in local field potentials from the subthalamic area (STN-LFPs) recorded through the deep brain macroelectrode during monopolar DBS of the subthalamic nucleus area (STN-DBS...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2008.01.023
更新日期：2008-07-30 00:00:00
abstract：:In order to examine the effect of chronic neuroleptics on spinal dopaminergic system, rats were treated with haloperidol (0.5 mg/kg IP) for 21 days and the monosynaptic mass reflex (MMR) as well as dopamine (DA) metabolism were investigated. MMR, recorded from ventral root L6 following supramaximal stimulation to ipsi...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(92)90240-x
更新日期：1992-01-01 00:00:00
abstract：:We showed previously that the number and time of spike-wave discharges (SWDs) were increased after intraperitoneal (i.p.) injection of lipopolysaccharide (LPS), an effect, which was completely abolished by cyclooxygenase-2 (COX-2) inhibitor indomethacin (IND) pretreatment in Wistar Albino Glaxo/Rijswijk (WAG/Rij) rats...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2014.03.003
更新日期：2014-05-01 00:00:00
abstract：:Adaptation of the frog, Rana temporaria, to a white background for 12 hr has resulted in an intense formaldehyde-induced fluorescence (FIF) in the neurons of the preoptic recess organ (PRO), paraventricular organ (PVO), nucleus infundibularis dorsalis (NID) and their basal processes permitting visualization of the PRO...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(82)90184-8
更新日期：1982-07-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2007.01.009
更新日期：2007-05-30 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/j.brainresbull.2019.08.010
更新日期：2019-11-01 00:00:00
abstract：:An opaque screen moving overhead provokes an escape response in the crab Chasmagnathus granulatus that habituates after a few presentations of the eliciting stimulus. Fifteen trials with a 180-s intertrial interval or 30 trials with a 90-s interval (strong training protocol) ensures long-term habituation (LTH) of the ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(96)00178-5
更新日期：1996-01-01 00:00:00
abstract：:Melatonin (Mel) has neuroprotective effects; however, its roles in hypoxic-ischemic brain damage (HIBD) and the underlying mechanisms remain unknown. We aimed to explore its roles and mechanisms in a HIBD rat model. We found that exogenous Mel treatment ameliorated HIBD-induced pathological changes, inhibited neuronal...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2020.07.011
更新日期：2020-10-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(82)90073-9
更新日期：1982-04-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2017.07.021
更新日期：2017-09-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(89)90143-3
更新日期：1989-09-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2016.09.011
更新日期：2016-10-01 00:00:00
abstract：:Patients with post-traumatic stress disorder (PTSD) tend to misinterpret innocuous stimuli as potential threats, possibly due to a conditioning provoked by traumatic episodes. Previous neuroimaging evidence has shown an abnormal activation of the amygdala and prefrontal cortex in PTSD patients during fear conditioning...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2015.03.001
更新日期：2015-05-01 00:00:00
abstract：:Ocular enucleation induces profound morphological alterations in central visual areas. However, little is known about the response of glial cells and possible inflammatory processes in visual brain areas resulting from eye enucleation. In this study, immunoblotting and immunostaining assays revealed increased expressi...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2013.12.001
更新日期：2014-02-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2020.09.019
更新日期：2020-12-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2004.03.008
更新日期：2004-07-15 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(86)90200-5
更新日期：1986-11-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(01)00735-3
更新日期：2002-01-15 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(82)90169-1
更新日期：1982-07-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(84)90223-5
更新日期：1984-01-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(93)90222-w
更新日期：1993-01-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2006.06.006
更新日期：2006-10-16 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(83)90144-2
更新日期：1983-04-01 00:00:00
abstract：:A microdialysis probe, implanted in the posterior hypothalamus (PH) was used to examine changes of extracellular norepinephrine (NE) in freely moving rats from which mean arterial pressure (mAP) and heart rate (HR) were continuously monitored. Artificial cerebrospinal fluid (aCSF) was pumped through the probe and 10-m...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(90)90117-i
更新日期：1990-10-01 00:00:00
abstract：:A number of procedures exist for the experimental induction of sodium appetite. With the exception of a low sodium diet, nearly all of these methods are invasive, requiring injections, surgery, or both. In addition to stimulating intake of concentrated salt, some of them produce substantial side-effects like reduced f...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2004.03.009
更新日期：2004-07-30 00:00:00
