Abstract：
:Protein degradation is an essential mechanism for maintaining proteostasis in response to internal and external perturbations. Disruption of this process is implicated in many human diseases. We present a new technique, QUAD (Quantification of Azidohomoalanine Degradation), to analyze the global degradation rates in tissues using a non-canonical amino acid and mass spectrometry. QUAD analysis reveals that protein stability varied within tissues, but discernible trends in the data suggest that cellular environment is a major factor dictating stability. Within a tissue, different organelles and protein functions were enriched with different stability patterns. QUAD analysis demonstrated that protein stability is enhanced with age in the brain but not in the liver. Overall, QUAD allows the first global quantitation of protein stability rates in tissues, which will allow new insights and hypotheses in basic and translational research.
journal_name
Sci Repjournal_title
Scientific reportsauthors
McClatchy DB,Martínez-Bartolomé S,Gao Y,Lavallée-Adam M,Yates JR 3rddoi
10.1038/s41598-020-72410-ysubject
Has Abstractpub_date
2020-09-29 00:00:00pages
15983issue
1issn
2045-2322pii
10.1038/s41598-020-72410-yjournal_volume
10pub_type
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