Abstract:
:Migrating cells move across diverse assemblies of extracellular matrix (ECM) that can be separated by micron-scale gaps. For membranes to protrude and reattach across a gap, actin filaments, which are relatively weak as single filaments, must polymerize outward from adhesion sites to push membranes towards distant sites of new adhesion. Here, using micropatterned ECMs, we identify T-Plastin, one of the most ancient actin bundling proteins, as an actin stabilizer that promotes membrane protrusions and enables bridging of ECM gaps. We show that T-Plastin widens and lengthens protrusions and is specifically enriched in active protrusions where F-actin is devoid of non-muscle myosin II activity. Together, our study uncovers critical roles of the actin bundler T-Plastin to promote protrusions and migration when adhesion is spatially-gapped.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Garbett D,Bisaria A,Yang C,McCarthy DG,Hayer A,Moerner WE,Svitkina TM,Meyer Tdoi
10.1038/s41467-020-18586-3subject
Has Abstractpub_date
2020-09-23 00:00:00pages
4818issue
1issn
2041-1723pii
10.1038/s41467-020-18586-3journal_volume
11pub_type
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