Abstract:
:Protein tyrosine phosphatases regulate a myriad of essential subcellular signaling events, yet they remain difficult to study in their native biophysical context. Here we develop a minimally disruptive optical approach to control protein tyrosine phosphatase 1B (PTP1B)-an important regulator of receptor tyrosine kinases and a therapeutic target for the treatment of diabetes, obesity, and cancer-and we use that approach to probe the intracellular function of this enzyme. Our conservative architecture for photocontrol, which consists of a protein-based light switch fused to an allosteric regulatory element, preserves the native structure, activity, and subcellular localization of PTP1B, affords changes in activity that match those elicited by post-translational modifications inside the cell, and permits experimental analyses of the molecular basis of optical modulation. Findings indicate, most strikingly, that small changes in the activity of PTP1B can cause large shifts in the phosphorylation states of its regulatory targets.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Hongdusit A,Zwart PH,Sankaran B,Fox JMdoi
10.1038/s41467-020-14567-8subject
Has Abstractpub_date
2020-02-07 00:00:00pages
788issue
1issn
2041-1723pii
10.1038/s41467-020-14567-8journal_volume
11pub_type
杂志文章abstract::The main advantage of organic transistors with dual gates/bases is that the threshold voltages can be set as a function of the applied second gate/base bias, which is crucial for the application in logic gates and integrated circuits. However, incorporating a dual gate/base structure into an ultra-short channel vertic...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18576-5
更新日期:2020-09-18 00:00:00
abstract::SecB chaperones assist protein export by binding both unfolded proteins and the SecA motor. Certain SecB homologs can also control toxin-antitoxin (TA) systems known to modulate bacterial growth in response to stress. In such TA-chaperone (TAC) systems, SecB assists the folding and prevents degradation of the antitoxi...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-08747-4
更新日期:2019-02-15 00:00:00
abstract::A disturbance of reactive oxygen species (ROS) homeostasis may cause the pathogenesis of many diseases. Inspired by natural photosynthesis, this work proposes a photo-driven H2-evolving liposomal nanoplatform (Lip NP) that comprises an upconversion nanoparticle (UCNP) that is conjugated with gold nanoparticles (AuNPs)...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-14413-x
更新日期:2020-01-27 00:00:00
abstract::Leads are a key feature of the Arctic ice pack during the winter owing to their substantial contribution to the surface energy balance. According to the present understanding, enhanced heat and moisture fluxes from high lead concentrations tend to produce more boundary layer clouds. However, described here in our comp...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-14074-5
更新日期:2020-01-10 00:00:00
abstract::Surface-supported isolated atoms in single-atom catalysts (SACs) are usually stabilized by diverse defects. The fabrication of high-metal-loading and thermally stable SACs remains a formidable challenge due to the difficulty of creating high densities of underpinning stable defects. Here we report that isolated Pt ato...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-08136-3
更新日期:2019-01-16 00:00:00
abstract::Standard units of measurement are required for the quantitative description of nature; however, few standard units have been established for genomics to date. Here, we have developed a synthetic DNA ladder that defines a quantitative standard unit that can measure DNA sequence abundance within a next-generation sequen...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17445-5
更新日期:2020-07-17 00:00:00
abstract::Osmoregulation is important for plant growth, development and response to environmental changes. SNF1-related protein kinase 2s (SnRK2s) are quickly activated by osmotic stress and are central components in osmotic stress and abscisic acid (ABA) signaling pathways; however, the upstream components required for SnRK2 a...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-14477-9
更新日期:2020-01-30 00:00:00
abstract::We establish a powerful poly(4-styrenesulfonate) (PSS)-treated strategy for sulfur vacancy healing in monolayer MoS2 to precisely and steadily tune its electronic state. The self-healing mechanism, in which the sulfur vacancies are healed spontaneously by the sulfur adatom clusters on the MoS2 surface through a PSS-in...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms15881
更新日期:2017-06-22 00:00:00
abstract::Circular RNAs (circRNAs) have recently gained substantial attention in the cancer research field where most, including the putative oncogene ciRS-7 (CDR1as), have been proposed to function as competitive endogenous RNAs (ceRNAs) by sponging specific microRNAs. Here, we report the first spatially resolved cellular expr...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18355-2
更新日期:2020-09-11 00:00:00
abstract::Silicon dioxide is one of the most abundant natural compounds. Polymorphs of SiO₂ and their phase transitions have long been a focus of great interest and intense theoretical and experimental pursuits. Here, compressing single-crystal coesite SiO₂ under hydrostatic pressures of 26-53 GPa at room temperature, we discov...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7630
更新日期:2015-03-20 00:00:00
abstract::The worldwide dissemination of metallo-β-lactamases (MBLs), mediating resistance to carbapenem antibiotics, is a major public health problem. The extent of dissemination of MBLs such as VIM-2, SPM-1 and NDM among Gram-negative pathogens cannot be explained solely based on the associated mobile genetic elements or the ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11615-w
更新日期:2019-08-09 00:00:00
abstract::CDKL5 deficiency disorder (CDD) is characterized by epilepsy, intellectual disability, and autistic features, and CDKL5-deficient mice exhibit a constellation of behavioral phenotypes reminiscent of the human disorder. We previously found that CDKL5 dysfunction in forebrain glutamatergic neurons results in deficits in...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-10689-w
更新日期:2019-06-14 00:00:00
abstract::The host-guest recognition between two macrocycles to form hierarchical non-intertwined ring-in-ring assemblies remains an interesting and challenging target in noncovalent synthesis. Herein, we report the design and characterization of a box-in-box assembly on the basis of host-guest radical-pairing interactions betw...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-07673-1
更新日期:2018-12-10 00:00:00
abstract::Imaging compound action potentials (CAPs) in peripheral nerves could help avoid side effects in neuromodulation by selective stimulation of identified fascicles. Existing methods have low resolution, limited imaging depth, or are invasive. Fast neural electrical impedance tomography (EIT) allows fascicular CAP imaging...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-20127-x
更新日期:2020-12-07 00:00:00
abstract::Identifying a bona fide population of cardiac stem cells (CSCs) is a critical step for developing cell-based therapies for heart failure patients. Previously, cardiac c-kit(+) cells were reported to be CSCs with a potential to become myocardial, endothelial and smooth muscle cells in vitro and after cardiac injury. He...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9701
更新日期:2015-10-30 00:00:00
abstract::Nitric oxide (NO) has important functions in biology and atmospheric chemistry as a toxin, signaling molecule, ozone depleting agent and the precursor of the greenhouse gas nitrous oxide (N2O). Although NO is a potent oxidant, and was available on Earth earlier than oxygen, it is unclear whether NO can be used by micr...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09268-w
更新日期:2019-03-20 00:00:00
abstract::Ultrasound sensors have wide applications across science and technology. However, improved sensitivity is required for both miniaturisation and increased spatial resolution. Here, we introduce cavity optomechanical ultrasound sensing, where dual optical and mechanical resonances enhance the ultrasound signal. We achie...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-08038-4
更新日期:2019-01-10 00:00:00
abstract::ASXL2 is frequently mutated in acute myeloid leukaemia patients with t(8;21). However, the roles of ASXL2 in normal haematopoiesis and the pathogenesis of myeloid malignancies remain unknown. Here we show that deletion of Asxl2 in mice leads to the development of myelodysplastic syndrome (MDS)-like disease. Asxl2-/- m...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms15456
更新日期:2017-06-08 00:00:00
abstract::Although a hydrophobic microtexture at a solid surface most often reflects rain owing to the presence of entrapped air within the texture, it is much more challenging to repel hot water. As it contacts a colder material, hot water generates condensation within the cavities at the solid surface, which eventually builds...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09456-8
更新日期:2019-03-29 00:00:00
abstract::The mathematics of gauge theories lies behind many of the most profound advances in physics in the past 200 years, from Maxwell's theory of electromagnetism to Einstein's theory of general relativity. More recently it has become clear that gauge theories also emerge in condensed matter, a prime example being the spin-...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11572
更新日期:2016-05-26 00:00:00
abstract::Non-Abelian quasiparticles have been predicted to exist in a variety of condensed matter systems. Their defining property is that an adiabatic braid between two of them results in a non-trivial change of the quantum state of the system. The simplest non-Abelian quasiparticles--the Majorana bound states--can occur in o...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms10395
更新日期:2016-01-21 00:00:00
abstract::The transcription factor BMAL1 is a core component of the molecular clock, regulating biological pathways that drive 24 h (circadian) rhythms in behaviour and physiology. The molecular clock has a profound influence on innate immune function, and circadian disruption is linked with increased incidence of multiple scle...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-02111-0
更新日期:2017-12-12 00:00:00
abstract::Novel atherosclerosis models are needed to guide clinical therapy. Here, we report an in vitro model of early atherosclerosis by fabricating and perfusing multi-layer arteriole-scale human tissue-engineered blood vessels (TEBVs) by plastic compression. TEBVs maintain mechanical strength, vasoactivity, and nitric oxide...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-19197-8
更新日期:2020-10-27 00:00:00
abstract::As the desire to explore opaque materials is ordinarily frustrated by multiple scattering of waves, attention has focused on the transmission matrix of the wave field. This matrix gives the fullest account of transmission and conductance and enables the control of the transmitted flux; however, it cannot address the f...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7893
更新日期:2015-04-20 00:00:00
abstract::Lithium- and sodium-rich layered transition-metal oxides have recently been attracting significant interest because of their large capacity achieved by additional oxygen-redox reactions. However, layered transition-metal oxides exhibit structural degradation such as cation migration, layer exfoliation or cracks upon d...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09409-1
更新日期:2019-05-16 00:00:00
abstract::The dynamics of many natural and artificial systems are well described as random walks on a network: the stochastic behaviour of molecules, traffic patterns on the internet, fluctuations in stock prices and so on. The vast literature on random walks provides many tools for computing properties such as steady-state pro...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms6121
更新日期:2014-10-14 00:00:00
abstract::Cytochrome P450 monooxygenases are versatile heme-thiolate enzymes that catalyze a wide range of reactions. Self-sufficient cytochrome P450 enzymes contain the redox partners in a single polypeptide chain. Here, we present the crystal structure of full-length CYP116B46, a self-sufficient P450. The continuous polypepti...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-16500-5
更新日期:2020-05-29 00:00:00
abstract::Natural killer (NK) cells are lymphocytes with important anti-tumour functions. Cytokine activation of NK cell glycolysis and oxidative phosphorylation (OXPHOS) are essential for robust NK cell responses. However, the mechanisms leading to this metabolic phenotype are unclear. Here we show that the transcription facto...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-04719-2
更新日期:2018-06-14 00:00:00
abstract::Nanofabricated and nanopatterned surfaces have revealed the sensitivity of cell adhesion to nanoscale variations in the spacing of adhesive ligands such as the tripeptide arginine-glycine-aspartic acid (RGD). To date, surface characterisation and cell adhesion are often examined in two separate experiments so that the...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-05837-7
更新日期:2018-08-20 00:00:00
abstract::Calcium ions (Ca(2+)) function as universal second messengers in eukaryotic cells, including immune cells. Ca(2+) is crucial for peripheral T-lymphocyte activation and effector functions, and influences thymocyte selection and motility in the developing thymus. However, the role of Ca(2+) signalling in early T-lymphoc...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms5814
更新日期:2014-09-12 00:00:00