Minimally disruptive optical control of protein tyrosine phosphatase 1B.

Abstract:

:Protein tyrosine phosphatases regulate a myriad of essential subcellular signaling events, yet they remain difficult to study in their native biophysical context. Here we develop a minimally disruptive optical approach to control protein tyrosine phosphatase 1B (PTP1B)-an important regulator of receptor tyrosine kinases and a therapeutic target for the treatment of diabetes, obesity, and cancer-and we use that approach to probe the intracellular function of this enzyme. Our conservative architecture for photocontrol, which consists of a protein-based light switch fused to an allosteric regulatory element, preserves the native structure, activity, and subcellular localization of PTP1B, affords changes in activity that match those elicited by post-translational modifications inside the cell, and permits experimental analyses of the molecular basis of optical modulation. Findings indicate, most strikingly, that small changes in the activity of PTP1B can cause large shifts in the phosphorylation states of its regulatory targets.

journal_name

Nat Commun

journal_title

Nature communications

authors

Hongdusit A,Zwart PH,Sankaran B,Fox JM

doi

10.1038/s41467-020-14567-8

subject

Has Abstract

pub_date

2020-02-07 00:00:00

pages

788

issue

1

issn

2041-1723

pii

10.1038/s41467-020-14567-8

journal_volume

11

pub_type

杂志文章
  • Vertical organic permeable dual-base transistors for logic circuits.

    abstract::The main advantage of organic transistors with dual gates/bases is that the threshold voltages can be set as a function of the applied second gate/base bias, which is crucial for the application in logic gates and integrated circuits. However, incorporating a dual gate/base structure into an ultra-short channel vertic...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-18576-5

    authors: Guo E,Wu Z,Darbandy G,Xing S,Wang SJ,Tahn A,Göbel M,Kloes A,Leo K,Kleemann H

    更新日期:2020-09-18 00:00:00

  • Structural insights into chaperone addiction of toxin-antitoxin systems.

    abstract::SecB chaperones assist protein export by binding both unfolded proteins and the SecA motor. Certain SecB homologs can also control toxin-antitoxin (TA) systems known to modulate bacterial growth in response to stress. In such TA-chaperone (TAC) systems, SecB assists the folding and prevents degradation of the antitoxi...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-08747-4

    authors: Guillet V,Bordes P,Bon C,Marcoux J,Gervais V,Sala AJ,Dos Reis S,Slama N,Mares-Mejía I,Cirinesi AM,Maveyraud L,Genevaux P,Mourey L

    更新日期:2019-02-15 00:00:00

  • Photosynthesis-inspired H2 generation using a chlorophyll-loaded liposomal nanoplatform to detect and scavenge excess ROS.

    abstract::A disturbance of reactive oxygen species (ROS) homeostasis may cause the pathogenesis of many diseases. Inspired by natural photosynthesis, this work proposes a photo-driven H2-evolving liposomal nanoplatform (Lip NP) that comprises an upconversion nanoparticle (UCNP) that is conjugated with gold nanoparticles (AuNPs)...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-14413-x

    authors: Wan WL,Tian B,Lin YJ,Korupalli C,Lu MY,Cui Q,Wan D,Chang Y,Sung HW

    更新日期:2020-01-27 00:00:00

  • Midwinter Arctic leads form and dissipate low clouds.

    abstract::Leads are a key feature of the Arctic ice pack during the winter owing to their substantial contribution to the surface energy balance. According to the present understanding, enhanced heat and moisture fluxes from high lead concentrations tend to produce more boundary layer clouds. However, described here in our comp...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-14074-5

    authors: Li X,Krueger SK,Strong C,Mace GG,Benson S

    更新日期:2020-01-10 00:00:00

  • Non defect-stabilized thermally stable single-atom catalyst.

    abstract::Surface-supported isolated atoms in single-atom catalysts (SACs) are usually stabilized by diverse defects. The fabrication of high-metal-loading and thermally stable SACs remains a formidable challenge due to the difficulty of creating high densities of underpinning stable defects. Here we report that isolated Pt ato...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-08136-3

    authors: Lang R,Xi W,Liu JC,Cui YT,Li T,Lee AF,Chen F,Chen Y,Li L,Li L,Lin J,Miao S,Liu X,Wang AQ,Wang X,Luo J,Qiao B,Li J,Zhang T

    更新日期:2019-01-16 00:00:00

  • A universal and independent synthetic DNA ladder for the quantitative measurement of genomic features.

    abstract::Standard units of measurement are required for the quantitative description of nature; however, few standard units have been established for genomics to date. Here, we have developed a synthetic DNA ladder that defines a quantitative standard unit that can measure DNA sequence abundance within a next-generation sequen...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-17445-5

    authors: Reis ALM,Deveson IW,Wong T,Madala BS,Barker C,Blackburn J,Marcellin E,Mercer TR

    更新日期:2020-07-17 00:00:00

  • A RAF-SnRK2 kinase cascade mediates early osmotic stress signaling in higher plants.

    abstract::Osmoregulation is important for plant growth, development and response to environmental changes. SNF1-related protein kinase 2s (SnRK2s) are quickly activated by osmotic stress and are central components in osmotic stress and abscisic acid (ABA) signaling pathways; however, the upstream components required for SnRK2 a...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-14477-9

    authors: Lin Z,Li Y,Zhang Z,Liu X,Hsu CC,Du Y,Sang T,Zhu C,Wang Y,Satheesh V,Pratibha P,Zhao Y,Song CP,Tao WA,Zhu JK,Wang P

    更新日期:2020-01-30 00:00:00

  • Poly(4-styrenesulfonate)-induced sulfur vacancy self-healing strategy for monolayer MoS2 homojunction photodiode.

    abstract::We establish a powerful poly(4-styrenesulfonate) (PSS)-treated strategy for sulfur vacancy healing in monolayer MoS2 to precisely and steadily tune its electronic state. The self-healing mechanism, in which the sulfur vacancies are healed spontaneously by the sulfur adatom clusters on the MoS2 surface through a PSS-in...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms15881

    authors: Zhang X,Liao Q,Liu S,Kang Z,Zhang Z,Du J,Li F,Zhang S,Xiao J,Liu B,Ou Y,Liu X,Gu L,Zhang Y

    更新日期:2017-06-22 00:00:00

  • Spatial expression analyses of the putative oncogene ciRS-7 in cancer reshape the microRNA sponge theory.

    abstract::Circular RNAs (circRNAs) have recently gained substantial attention in the cancer research field where most, including the putative oncogene ciRS-7 (CDR1as), have been proposed to function as competitive endogenous RNAs (ceRNAs) by sponging specific microRNAs. Here, we report the first spatially resolved cellular expr...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-18355-2

    authors: Kristensen LS,Ebbesen KK,Sokol M,Jakobsen T,Korsgaard U,Eriksen AC,Hansen TB,Kjems J,Hager H

    更新日期:2020-09-11 00:00:00

  • Polymorphic phase transition mechanism of compressed coesite.

    abstract::Silicon dioxide is one of the most abundant natural compounds. Polymorphs of SiO₂ and their phase transitions have long been a focus of great interest and intense theoretical and experimental pursuits. Here, compressing single-crystal coesite SiO₂ under hydrostatic pressures of 26-53 GPa at room temperature, we discov...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms7630

    authors: Hu QY,Shu JF,Cadien A,Meng Y,Yang WG,Sheng HW,Mao HK

    更新日期:2015-03-20 00:00:00

  • Protein determinants of dissemination and host specificity of metallo-β-lactamases.

    abstract::The worldwide dissemination of metallo-β-lactamases (MBLs), mediating resistance to carbapenem antibiotics, is a major public health problem. The extent of dissemination of MBLs such as VIM-2, SPM-1 and NDM among Gram-negative pathogens cannot be explained solely based on the associated mobile genetic elements or the ...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-11615-w

    authors: López C,Ayala JA,Bonomo RA,González LJ,Vila AJ

    更新日期:2019-08-09 00:00:00

  • Altered NMDAR signaling underlies autistic-like features in mouse models of CDKL5 deficiency disorder.

    abstract::CDKL5 deficiency disorder (CDD) is characterized by epilepsy, intellectual disability, and autistic features, and CDKL5-deficient mice exhibit a constellation of behavioral phenotypes reminiscent of the human disorder. We previously found that CDKL5 dysfunction in forebrain glutamatergic neurons results in deficits in...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-10689-w

    authors: Tang S,Terzic B,Wang IJ,Sarmiento N,Sizov K,Cui Y,Takano H,Marsh ED,Zhou Z,Coulter DA

    更新日期:2019-06-14 00:00:00

  • Molecular Russian dolls.

    abstract::The host-guest recognition between two macrocycles to form hierarchical non-intertwined ring-in-ring assemblies remains an interesting and challenging target in noncovalent synthesis. Herein, we report the design and characterization of a box-in-box assembly on the basis of host-guest radical-pairing interactions betw...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-07673-1

    authors: Cai K,Lipke MC,Liu Z,Nelson J,Cheng T,Shi Y,Cheng C,Shen D,Han JM,Vemuri S,Feng Y,Stern CL,Goddard WA 3rd,Wasielewski MR,Stoddart JF

    更新日期:2018-12-10 00:00:00

  • Imaging fascicular organization of rat sciatic nerves with fast neural electrical impedance tomography.

    abstract::Imaging compound action potentials (CAPs) in peripheral nerves could help avoid side effects in neuromodulation by selective stimulation of identified fascicles. Existing methods have low resolution, limited imaging depth, or are invasive. Fast neural electrical impedance tomography (EIT) allows fascicular CAP imaging...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-20127-x

    authors: Ravagli E,Mastitskaya S,Thompson N,Iacoviello F,Shearing PR,Perkins J,Gourine AV,Aristovich K,Holder D

    更新日期:2020-12-07 00:00:00

  • Resident c-kit(+) cells in the heart are not cardiac stem cells.

    abstract::Identifying a bona fide population of cardiac stem cells (CSCs) is a critical step for developing cell-based therapies for heart failure patients. Previously, cardiac c-kit(+) cells were reported to be CSCs with a potential to become myocardial, endothelial and smooth muscle cells in vitro and after cardiac injury. He...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms9701

    authors: Sultana N,Zhang L,Yan J,Chen J,Cai W,Razzaque S,Jeong D,Sheng W,Bu L,Xu M,Huang GY,Hajjar RJ,Zhou B,Moon A,Cai CL

    更新日期:2015-10-30 00:00:00

  • Nitric oxide-dependent anaerobic ammonium oxidation.

    abstract::Nitric oxide (NO) has important functions in biology and atmospheric chemistry as a toxin, signaling molecule, ozone depleting agent and the precursor of the greenhouse gas nitrous oxide (N2O). Although NO is a potent oxidant, and was available on Earth earlier than oxygen, it is unclear whether NO can be used by micr...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-09268-w

    authors: Hu Z,Wessels HJCT,van Alen T,Jetten MSM,Kartal B

    更新日期:2019-03-20 00:00:00

  • Precision ultrasound sensing on a chip.

    abstract::Ultrasound sensors have wide applications across science and technology. However, improved sensitivity is required for both miniaturisation and increased spatial resolution. Here, we introduce cavity optomechanical ultrasound sensing, where dual optical and mechanical resonances enhance the ultrasound signal. We achie...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-08038-4

    authors: Basiri-Esfahani S,Armin A,Forstner S,Bowen WP

    更新日期:2019-01-10 00:00:00

  • Loss of Asxl2 leads to myeloid malignancies in mice.

    abstract::ASXL2 is frequently mutated in acute myeloid leukaemia patients with t(8;21). However, the roles of ASXL2 in normal haematopoiesis and the pathogenesis of myeloid malignancies remain unknown. Here we show that deletion of Asxl2 in mice leads to the development of myelodysplastic syndrome (MDS)-like disease. Asxl2-/- m...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms15456

    authors: Li J,He F,Zhang P,Chen S,Shi H,Sun Y,Guo Y,Yang H,Man N,Greenblatt S,Li Z,Guo Z,Zhou Y,Wang L,Morey L,Williams S,Chen X,Wang QT,Nimer SD,Yu P,Wang QF,Xu M,Yang FC

    更新日期:2017-06-08 00:00:00

  • Two recipes for repelling hot water.

    abstract::Although a hydrophobic microtexture at a solid surface most often reflects rain owing to the presence of entrapped air within the texture, it is much more challenging to repel hot water. As it contacts a colder material, hot water generates condensation within the cavities at the solid surface, which eventually builds...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-09456-8

    authors: Mouterde T,Lecointre P,Lehoucq G,Checco A,Clanet C,Quéré D

    更新日期:2019-03-29 00:00:00

  • A spin-liquid with pinch-line singularities on the pyrochlore lattice.

    abstract::The mathematics of gauge theories lies behind many of the most profound advances in physics in the past 200 years, from Maxwell's theory of electromagnetism to Einstein's theory of general relativity. More recently it has become clear that gauge theories also emerge in condensed matter, a prime example being the spin-...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms11572

    authors: Benton O,Jaubert LD,Yan H,Shannon N

    更新日期:2016-05-26 00:00:00

  • Manipulating Majorana zero modes on atomic rings with an external magnetic field.

    abstract::Non-Abelian quasiparticles have been predicted to exist in a variety of condensed matter systems. Their defining property is that an adiabatic braid between two of them results in a non-trivial change of the quantum state of the system. The simplest non-Abelian quasiparticles--the Majorana bound states--can occur in o...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms10395

    authors: Li J,Neupert T,Bernevig BA,Yazdani A

    更新日期:2016-01-21 00:00:00

  • Loss of the molecular clock in myeloid cells exacerbates T cell-mediated CNS autoimmune disease.

    abstract::The transcription factor BMAL1 is a core component of the molecular clock, regulating biological pathways that drive 24 h (circadian) rhythms in behaviour and physiology. The molecular clock has a profound influence on innate immune function, and circadian disruption is linked with increased incidence of multiple scle...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-017-02111-0

    authors: Sutton CE,Finlay CM,Raverdeau M,Early JO,DeCourcey J,Zaslona Z,O'Neill LAJ,Mills KHG,Curtis AM

    更新日期:2017-12-12 00:00:00

  • Modeling early stage atherosclerosis in a primary human vascular microphysiological system.

    abstract::Novel atherosclerosis models are needed to guide clinical therapy. Here, we report an in vitro model of early atherosclerosis by fabricating and perfusing multi-layer arteriole-scale human tissue-engineered blood vessels (TEBVs) by plastic compression. TEBVs maintain mechanical strength, vasoactivity, and nitric oxide...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-19197-8

    authors: Zhang X,Bishawi M,Zhang G,Prasad V,Salmon E,Breithaupt JJ,Zhang Q,Truskey GA

    更新日期:2020-10-27 00:00:00

  • Universal structure of transmission eigenchannels inside opaque media.

    abstract::As the desire to explore opaque materials is ordinarily frustrated by multiple scattering of waves, attention has focused on the transmission matrix of the wave field. This matrix gives the fullest account of transmission and conductance and enables the control of the transmitted flux; however, it cannot address the f...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms7893

    authors: Davy M,Shi Z,Park J,Tian C,Genack AZ

    更新日期:2015-04-20 00:00:00

  • Coulombic self-ordering upon charging a large-capacity layered cathode material for rechargeable batteries.

    abstract::Lithium- and sodium-rich layered transition-metal oxides have recently been attracting significant interest because of their large capacity achieved by additional oxygen-redox reactions. However, layered transition-metal oxides exhibit structural degradation such as cation migration, layer exfoliation or cracks upon d...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-09409-1

    authors: Mortemard de Boisse B,Reynaud M,Ma J,Kikkawa J,Nishimura SI,Casas-Cabanas M,Delmas C,Okubo M,Yamada A

    更新日期:2019-05-16 00:00:00

  • A scaling law for random walks on networks.

    abstract::The dynamics of many natural and artificial systems are well described as random walks on a network: the stochastic behaviour of molecules, traffic patterns on the internet, fluctuations in stock prices and so on. The vast literature on random walks provides many tools for computing properties such as steady-state pro...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms6121

    authors: Perkins TJ,Foxall E,Glass L,Edwards R

    更新日期:2014-10-14 00:00:00

  • Structural insight into the electron transfer pathway of a self-sufficient P450 monooxygenase.

    abstract::Cytochrome P450 monooxygenases are versatile heme-thiolate enzymes that catalyze a wide range of reactions. Self-sufficient cytochrome P450 enzymes contain the redox partners in a single polypeptide chain. Here, we present the crystal structure of full-length CYP116B46, a self-sufficient P450. The continuous polypepti...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-16500-5

    authors: Zhang L,Xie Z,Liu Z,Zhou S,Ma L,Liu W,Huang JW,Ko TP,Li X,Hu Y,Min J,Yu X,Guo RT,Chen CC

    更新日期:2020-05-29 00:00:00

  • Amino acid-dependent cMyc expression is essential for NK cell metabolic and functional responses in mice.

    abstract::Natural killer (NK) cells are lymphocytes with important anti-tumour functions. Cytokine activation of NK cell glycolysis and oxidative phosphorylation (OXPHOS) are essential for robust NK cell responses. However, the mechanisms leading to this metabolic phenotype are unclear. Here we show that the transcription facto...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-04719-2

    authors: Loftus RM,Assmann N,Kedia-Mehta N,O'Brien KL,Garcia A,Gillespie C,Hukelmann JL,Oefner PJ,Lamond AI,Gardiner CM,Dettmer K,Cantrell DA,Sinclair LV,Finlay DK

    更新日期:2018-06-14 00:00:00

  • Monolayer surface chemistry enables 2-colour single molecule localisation microscopy of adhesive ligands and adhesion proteins.

    abstract::Nanofabricated and nanopatterned surfaces have revealed the sensitivity of cell adhesion to nanoscale variations in the spacing of adhesive ligands such as the tripeptide arginine-glycine-aspartic acid (RGD). To date, surface characterisation and cell adhesion are often examined in two separate experiments so that the...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-05837-7

    authors: Lu X,Nicovich PR,Zhao M,Nieves DJ,Mollazade M,Vivekchand SRC,Gaus K,Gooding JJ

    更新日期:2018-08-20 00:00:00

  • Loss of IP3R-dependent Ca2+ signalling in thymocytes leads to aberrant development and acute lymphoblastic leukemia.

    abstract::Calcium ions (Ca(2+)) function as universal second messengers in eukaryotic cells, including immune cells. Ca(2+) is crucial for peripheral T-lymphocyte activation and effector functions, and influences thymocyte selection and motility in the developing thymus. However, the role of Ca(2+) signalling in early T-lymphoc...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms5814

    authors: Ouyang K,Leandro Gomez-Amaro R,Stachura DL,Tang H,Peng X,Fang X,Traver D,Evans SM,Chen J

    更新日期:2014-09-12 00:00:00