Abstract:
ETHNOPHARMACOLOGICAL RELEVANCE:Kanglaite (KLT) is an active extract of the Coix lacryma-jobi seed, which can benefit Qi and nourish Yin, and disperse the accumulation of evils. It is used as a biphasic broad-spectrum anti-cancer drug, and shows synergistic effects with radiotherapy and chemotherapy. However, the mechanism of KLT combined with cisplatin (CDDP) against hepatocellular carcinoma (HCC) has not been elucidated. AIM OF THE STUDY:The aim of present study was to investigate the potential synergistic effects of KLT and CDDP on HepG2 cells, discussing the possible mechanisms from the perspective of CKLF1 and NF-κB mediated inflammatory response and chemoresistance, and the involvement of drug efflux transporters. MATERIALS AND METHODS:CDDP injured HepG2 cells were used to investigate the effects of KLT on chemotherapeutics treated HCC. Effects of KLT pretreatment on CDDP injured HepG2 cells were determined by MTT, wound healing assay, and transwell assay. Expression of chemokine-like factor 1 (CKLF1) and activation of nuclear factor κB (NF-κB) were examined by qPCR, western blot, and immunofluorescence staining. Furthermore, to study the role of CKLF1 in KLT mediated effects on this CDDP injured HCC cell model, HepG2 cells overexpressed with CKLF1 gene were used. Cell viability and NF-κB activation were investigated. Moreover, TNF-α and IL-1β levels were measured by Elisa analysis and western blot to evaluate the inflammatory response. Additionally, ATP-binding cassette (ABC) drug efflux transporters, MDR1, MRP2, and BCRP were also determined in present study. RESULTS:KLT pretreatment followed by CDDP treatment was found to show synergistic effects, which showed by decreased cell viability, migration and invasion ability of HepG2 cells. Expression of CKLF1 enhanced significantly in CDDP treated HepG2 cells, and KLT decreased this elevation obviously. Furthermore, CDDP activated NF-κΒ and promoted translocation of NF-κB toward the nucleus. KLT inhibited the activation of NF-κΒ, which sensitized cancer cells. Overexpression of CKLF1 reversed the effects of KLT on CDDP injured HepG2 cells, which exhibited by increased cell viability and enhanced activation of NF-κΒ. CDDP induced NF-κΒ activation could also lead to excessive inflammatory response, and KLT can suppress the aggravating inflammation which may be beneficial for tumor progression. Furthermore, we found that ABC drug efflux transporters MDR1, MRP2, and BCRP in CDDP treated HepG2 cells were decreased when pretreated with KLT. CONCLUSIONS:KLT pretreatment may increase the effects of CDDP on HepG2 cells, by exhibiting cooperative effects on suppression of HepG2 cells. The mechanisms may partly by inhibiting CKLF1 mediated NF-κB pathway, which may contribute to inflammation of tumor microenvironment and chemoresistance of CDDP. Inhibition of transporter-mediated drug efflux is also involved in KLT mediated sensitization effects of CDDP.
journal_name
J Ethnopharmacoljournal_title
Journal of ethnopharmacologyauthors
Chen C,Ai QD,Wei YHdoi
10.1016/j.jep.2020.113388subject
Has Abstractpub_date
2021-01-10 00:00:00pages
113388eissn
0378-8741issn
1872-7573pii
S0378-8741(20)33273-6journal_volume
264pub_type
杂志文章abstract::Cecropia obtusifolia (Cecropiaceae) is a species from tropical America and its leaves are used in traditional medicine for the treatment of diabetes and as an anti-inflammatory agent. In the present study, the analgesic, anti-inflammatory and central nervous system depressant effects of the aqueous extract from the le...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(01)00253-7
更新日期:2001-08-01 00:00:00
abstract::In the biological sciences the use of medicinal plants in indigenous cultures is commonly seen as being based on a long tradition ('traditional medicine'). However, under normal circumstances, ethnobotanical studies cannot provide evidence on the antiquity of specific uses for medicinal plants since oral traditions ha...
journal_title:Journal of ethnopharmacology
pub_type: 历史文章,杂志文章
doi:10.1016/s0378-8741(03)00188-0
更新日期:2003-10-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Gastrodia elata Blume (Fam. Orchidaceae) is a traditional Chinese herbal medicine for treating headaches, dizziness, tetanus, epilepsy, and numbness of the limbs, which suggests that it has neuroprotective effect. AIM OF THE STUDY:To validate the neuroprotection of Gastrodia elata in pre...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2011.08.075
更新日期:2011-10-31 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Organoleptic properties, and more specifically chemosensory cues, have been shown to guide therapeutic applications of medicinal plants. Humoral qualities, on the other hand, are widely believed to be an abstract concept, mainly applied post hoc to validate therapy. However, the nexus bet...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2017.01.027
更新日期:2017-02-23 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Jammu & Kashmir (J&K) is a predominantly Himalayan state in the north-western part of India. It has three geographically distinct divisions viz., Jammu, Kashmir and Ladakh, which are immensely rich in their biological and cultural diversity. Medicinal plants are an important element of in...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.jep.2014.06.029
更新日期:2014-09-11 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:The Natal gwarri or Natal ebony (Euclea natalensis A.DC.) is a deciduous tree found widespread throughout southern Africa, especially in Kwazulu-Natal and the southern cost. It has been widely used by indigenous communities such as the Zulus, Tsongas and Vendas for symptoms related to tub...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2016.10.060
更新日期:2016-12-24 00:00:00
abstract::The anticholinergic action of Swertia japonica, used in Japan as a bitter stomachic, was examined using in vivo experiments in rats in order to substantiate the presence or absence of antispasmodic properties. The methanol extract of Swertia japonica was found to be an effective anticholinergic given orally. Fractiona...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/0378-8741(91)90157-9
更新日期:1991-05-01 00:00:00
abstract::The effect of ethanol extract of Carpolobia lutea leaves on experimentally induced diarrhoea and ulcers was studied in rodents. The extract (245-735 mg/kg) inhibited small intestinal transit time (15.10-45.03%), castor oil-induced diarrhoea (25.69-43.54%) and fluid accumulation (7.53-34.15%), respectively, as well as ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2007.01.019
更新日期:2007-05-22 00:00:00
abstract::Ethnobotanists often utilize predictive models to analyze the potential of indigenously used medicinal plants. The most common of these prognostic models is the informant consensus model. This study evaluates use of this model through the analytical ethnopharmacology of Manus Province, Papua New Guinea (PNG). The info...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2005.12.005
更新日期:2006-06-15 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Previous ethnobotanical surveys from the north Andean part of Chile, where different ethnic groups are co-existing, with the preeminence of Aymara and Atacama traditions, revealed an extensive domestic use of the local flora. In these communities, traditional medicinal uses are mainly rel...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2018.10.029
更新日期:2019-03-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Xiaochaihutang (XCHT) is a traditional Chinese medicine prescription for thousand years in China. Our previous researches show that XCHT has antidepressant-like effects in several depression models, but effect and mechanism of XCHT in perimenopausal depression are still vague. AIM OF THE...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2019.112318
更新日期:2020-02-10 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Five herbs, Curcuma longa L. (CL), Scutellaria baicalensis Georgi (SBC), Scutellaria barbata D. Don (SBB), Hedyotis diffusa Willd. (HD) and Solanum nigrum L. (SN), are often prescribed in the polyherbal formulas for cancer treatment by traditional Chinese medicine (TCM) practitioners. The...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2013.04.014
更新日期:2013-06-21 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Phyllanthus niruri is a well-known plant for its therapeutic purposes to treat various diseases, being widely used by the population, mainly by women. However, there is no scientific confirmation of the effects of use during pregnancy. AIM OF THE STUDY:Evaluating the effect of Phyllanthu...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2020.112728
更新日期:2020-05-23 00:00:00
abstract:ETHNOPHARMACOLOGY RELEVANCE:Danggui Buxue Tang has been used in China to treat clinical anemia for more than 800 years. However, there is no scientific report on its effect on bone marrow stromal cells. AIM OF THE STUDY:Here, we aimed to explore the effect of Danggui Buxue Tang on bone marrow stromal cell adhesion and...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2018.11.018
更新日期:2019-03-01 00:00:00
abstract::Devil's Club, Oplopanax horridus (OH), is a widely used folk medicine in Alaska and British Columbia for treating a variety of ailments including arthritis, fever and diabetes. HPLC profiling shows that numerous compounds are present in the 70% ethanolic extract of OH dry root bark powder. OH extract inhibited K562, H...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2006.05.018
更新日期:2006-11-24 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Calculus bovis (C. bovis), a widespread known traditional animal drug in China and Japan, has been widely used for a long time to treat various diseases, including high fever, convulsion and stroke. The aim of the present paper is to comprehensively review knowledge about C. bovis in term...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.jep.2020.112649
更新日期:2020-05-23 00:00:00
abstract::Various aspects of medicinal plant research, i.e. collection of data, screening for biological activity and isolation and identification of active compounds are briefly discussed with special reference to phytochemical aspects. Based upon this some topics for further methodological research are presented. A strategy f...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/0378-8741(89)90044-5
更新日期:1989-02-01 00:00:00
abstract::Many cardiovascular diseases are associated with an increase in blood platelet activity. In Morocco, parsley (Petroselinum crispum, Apiaceae) is one of the medicinal herbs used to treat cardiovascular diseases such as arterial hypertension. In this study, crude aqueous extract (CAE) of parsley was evaluated for its an...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2009.05.014
更新日期:2009-08-17 00:00:00
abstract:ETHNOPHARMACOLOGICAL IMPORTANCE:Oral administration of alcoholic decoctions of Aristolochia ringens is used extensively by Traditional Medicine Practitioners (TMP) of Yoruba heritage in South west, Nigeria for the management of diabetes and its associated complications. AIM OF THE STUDY:To assess the potentials of roo...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2016.02.002
更新日期:2016-04-22 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Chiranthodendron pentadactylon Larreat is frequently used in Mexican traditional medicine as well as in Guatemalan for several medicinal purposes, including their use in the control of diarrhea. AIM OF THE STUDY:This work was undertaken to obtain additional information that support the t...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2012.07.039
更新日期:2012-09-28 00:00:00
abstract::The Dominican Republic has an extension of 48 442 km2. It occupies the eastern portion of the island of Hispaniola and is the second in size within the Greater Antilles. The floral resources of the Island have been extensively exploited without an adequate management policy. This has led to the destruction of about 90...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/0378-8741(95)01356-3
更新日期:1996-04-01 00:00:00
abstract::In an ethnopharmacological screening of selected medicinal plants used in Nepal, methanol extracts from 20 plant species were assayed for activity against eleven strains of bacteria and four strains of fungi. Duplicate assays were conducted with and without exposure to ultraviolet (UV)-A radiation to test for light-ac...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/0378-8741(95)01335-0
更新日期:1996-02-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Achyrocline satureoides is a medium-sized South American indigenous herb, commonly known as "Marcela" or "Macela". The infusion obtained from inflorescences of this plant is widely used in Brazilian folk medicine as an antispasmodic, anti-inflammatory, hypoglycemic, and hypocholesterolemi...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2010.05.014
更新日期:2010-07-20 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Traditional polyherbal preparations have been utilized in Sri Lanka since ancient times and have gained a wide acceptance throughout the country. Although an extensive body of evidence supports the use of traditional herbal mixtures in the treatment of diabetes mellitus, only a few polyhe...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2020.113613
更新日期:2021-03-01 00:00:00
abstract:AIM OF THE STUDY:The leaves of Costus pictus D. Don were used extensively for its antihyperglycemic activity by the people in Kerala, India. In the present study, the antihyperglycemic and insulin secretory activity of an aqueous extract of Costus pictus leaf extract was investigated in streptozotocin induced diabetic ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2009.03.026
更新日期:2009-06-25 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Waltheria indica L. is traditionally used in several countries against inflammatory related diseases and cancer, mainly as a decoction of the aerial parts. AIM OF THE STUDY:The transcription factor NF-κB is known to induce tumor promotion and progression and is considered a major player ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2017.03.048
更新日期:2017-05-05 00:00:00
abstract::The Indians of the northwest Amazon respect age and try to care for those suffering from senile dementia. At least 25 species of plants are employed in treating Alzheimer's disease or similar problems. The plants are administered orally as teas of the leaves or roots and over a period of several days to numerous weeks...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/0378-8741(93)90007-r
更新日期:1993-03-01 00:00:00
abstract::The feeding of a fish extract, prepared by extracting whole fish with 80% ethanol, prevented abdominal, thoracic aorta and coronary atheroma in cholesterol-fed rabbits. Daily fish extract feeding reduced serum cholesterol and LDL-cholesterol levels by 41.8 and 38.0%, respectively. In addition, triglyceride levels were...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/0378-8741(94)90058-2
更新日期:1994-01-01 00:00:00
abstract::The immunomodulatory effect of Ganoderma lucidum immunomodulating substance (GLIS) on macrophages has been investigated as part of on-going research into the anti-cancer properties of Ganoderma lucidum. Proliferation of bone marrow macrophages (BMMs) was enhanced by GLIS in a dose-dependent manner. Microscopic examina...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2007.03.035
更新日期:2007-07-25 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Bergenin is a well-known active compound that exhibits antioxidant, antiarrhythmic, hepatoprotective, and anti-inflammatory activities. However, the resource reserve of Rodgersia sambucifolia, one of the main raw materials for extracting bergenin, have sharply declined, and the bergenin c...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2019.112201
更新日期:2020-01-30 00:00:00