Immunomodulation of RAW264.7 macrophages by GLIS, a proteopolysaccharide from Ganoderma lucidum.

Abstract:

:The immunomodulatory effect of Ganoderma lucidum immunomodulating substance (GLIS) on macrophages has been investigated as part of on-going research into the anti-cancer properties of Ganoderma lucidum. Proliferation of bone marrow macrophages (BMMs) was enhanced by GLIS in a dose-dependent manner. Microscopic examination revealed that numerous GLIS-treated RAW264.7 macrophages were enlarged and formed pseudopodia. Exposure of RAW264.7 macrophages to GLIS resulted in significant increases in NO production, induction of cellular respiratory burst activity, and increased levels of IL-1beta, IL-12p35 and IL-12p40 gene expression. Our data indicate that GLIS activates the immune system by modulating cytokine production.

journal_name

J Ethnopharmacol

authors

Ji Z,Tang Q,Zhang J,Yang Y,Jia W,Pan Y

doi

10.1016/j.jep.2007.03.035

subject

Has Abstract

pub_date

2007-07-25 00:00:00

pages

445-50

issue

3

eissn

0378-8741

issn

1872-7573

pii

S0378-8741(07)00180-8

journal_volume

112

pub_type

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