Determination of antibiotics and detergent residues in decellularized tissue-engineered heart valves using LC-MS/MS.

Abstract:

:Residual chemicals that are presented during tissue processing in human tissue banks can be a risk for the allograft recipient. Determine the residual concentrations of the antibiotics and detergent used in the process of human decellularized tissue-engineered heart valves stored in isotonic saline solution up to 18 months. A total of 24 human decellularized allografts were stored in sterile sodium chloride and analyzed immediately after the decellularization process (0 months) and after storage for 6, 12, and 18 months, which includes the use of sodium dodecyl sulfate (SDS) and antibiotics (cefoxitin, vancomycin hydrochloride, lincomycin hydrochloride, polymyxin B sulfate). These valves were used for suitability tests, the zone of inhibition evaluation, and direct contact cytotoxicity assay. The stock solution from 32 valves was used for LC-MS/MS analysis of antibiotics and SDS. Tissue samples from decellularized valves showed a zone of inhibition formation for S. aureus and B. subtilis, suggesting the presence of an inhibitory molecule in the tissue. Cytotoxicity tests were negative. Polymyxin B, vancomycin, and SDS were detected and quantified in human decellularized aortic and pulmonary allografts during all periods of the study. There were no traces of residual cefoxitin and lincomycin in the tissue stock solution. We found residual concentrations of the antibiotics and detergent used in the process of human decellularized tissue-engineered heart valves stored in isotonic saline solution up to 18 months.

journal_name

Cell Tissue Bank

journal_title

Cell and tissue banking

authors

Kraft L,Ribeiro VST,de Nazareno Wollmann LCF,Suss PH,Tuon FF

doi

10.1007/s10561-020-09856-x

subject

Has Abstract

pub_date

2020-12-01 00:00:00

pages

573-584

issue

4

eissn

1389-9333

issn

1573-6814

pii

10.1007/s10561-020-09856-x

journal_volume

21

pub_type

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