Abstract：
:Human amniotic membrane (HAM) is used as an allograft in regenerative medicine or as a source of pluripotent cells for stem cell research. Various decontamination protocols and solutions are used to sterilize HAM before its application, but little is known about the toxicity of disinfectants on HAM cells. In this study, we tested two decontamination solutions, commercial (BASE·128) and laboratory decontamination solution (LDS), with an analogous content of antimycotic/antibiotics for their cytotoxic effect on HAM epithelial (EC) and mesenchymal stromal cells (MSC). HAM was processed in a standard way, placed on nitrocellulose scaffold, and decontaminated, following three protocols: (1) 6 h, 37 °C; (2) 24 h, room temperature; (3) 24 h, 4 °C. The viability of EC was assessed via trypan blue staining. The apoptotic cells were detected using terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL). The mean % (±SD) of dead EC (%DEC) from six fresh placentas was 12.9 ± 18.1. Decontamination increased %DEC compared to culture medium. Decontamination with BASE·128 for 6 h, 37 °C led to the highest EC viability (81.7%). Treatment with LDS at 24 h, 4 °C resulted in the lowest EC viability (55.9%) in the set. MSC were more affected by apoptosis than EC. Although the BASE·128 expresses lower toxicity compared to LDS, we present LDS as an alternative decontamination solution with a satisfactory preservation of cell viability. The basic formula of LDS will be optimised by enrichment with nutrient components, such as glucose or vitamins, to improve cell viability.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Smeringaiova I,Trosan P,Mrstinova MB,Matecha J,Burkert J,Bednar J,Jirsova Kdoi
10.1007/s10561-017-9636-3subject
Has Abstractpub_date
2017-09-01 00:00:00pages
413-423issue
3eissn
1389-9333issn
1573-6814pii
10.1007/s10561-017-9636-3journal_volume
18pub_type
杂志文章abstract：:Recently Bulgarian Bone Marrow Donors Registry (BBMDR) has been established and since August 2005 it has been a member of Bone Marrow Donors Worldwide. Currently the number of healthy donors included in the BBMDR is relatively low. All donors included in the BBMDR are typed for HLA-A, -B, -DRB loci. Phylogenetic analy...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-007-9046-z
更新日期：2008-12-01 00:00:00
abstract：:During August and September of 2013, temperature data loggers were shipped to and from an AATB accredited and FDA registered allograft tissue processing facility in Belgrade, MT (Bacterin International, Inc.) to five warm climate cities (Dallas, TX, El Paso, TX, New Orleans, LA, Phoenix, AZ, and Tampa, FL). Shipping d...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-016-9578-1
更新日期：2016-12-01 00:00:00
abstract：:The purpose of the present research was: 1. To investigate the opportunity of application of allogen osteoplastic materials such as Lioplast, received in Samara Tissue Bank of Samara State Medical University. 2. To work out a new technique of producing lyophilized allogen osteoplastic materials, such as Lioplast, with...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-004-7254-3
更新日期：2005-01-01 00:00:00
abstract：BACKGROUND:The role of bone and tissue banking is well known to meet the ever-growing need of bone and soft tissue allografts. Strict guidelines have been established to ensure high standard and minimize complications related to bone transplantation. METHODS:The Bone Bank in King Faisal Specialist Hospital and Researc...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09868-7
更新日期：2020-10-04 00:00:00
abstract：:Outcomes for patients with metastatic and recurrent osteosarcoma remain poor and a better understanding of the biology of this malignancy is critical to the development of prognostic biomarkers and novel therapies. The purpose of this study was to establish a biobank of osteosarcoma which has the potential of monitori...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09831-6
更新日期：2020-09-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) have vast potential in cell therapy, and are experimentally used in the clinic. Therefore, it is critical to find a serum- and xeno-free cryopreservation method. The aim of this study was to compare two serum- and xeno-free cryoprotectants for MSCs. Adipose tissue MSCs (Ad-MSCs) and bone ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9463-8
更新日期：2015-06-01 00:00:00
abstract：:The Israel National Skin Bank (INSB) was founded jointly by the Israel Defense Forces (IDF) Medical Corps and the Ministry of Health in 1986. The prime purpose of the Skin Bank is to treat burn victims incurred at war or during mass casualty incidences. The INSB Protocol is comprised of international skin bank protoco...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1010183719662
更新日期：2000-01-01 00:00:00
abstract：:Repair of damaged cartilage usually requires replacement tissue or substitute material. Tissue engineering is a promising means to produce replacement cartilage from autologous or allogeneic cell sources. Scaffolds provide a three-dimensional (3D) structure that is essential for chondrocyte function and synthesis of c...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-005-5810-0
更新日期：2005-01-01 00:00:00
abstract：:Cryopreserved (CryoPA) and Glycerol-preserved (GPA) skin allografts are commonly used in the treatment of severe burn injuries. However, comparable data on their differences in clinical outcome is scarce. This retrospective review aims to study the effect of allograft viability on clinical outcomes. The records of 48 ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9254-4
更新日期：2012-06-01 00:00:00
abstract：:In order to solve some of the problems that are affecting tissue banking activities in the world, a new program/project proposal could be prepared by the IAEA and interested Member States in order to implement it in 2009. The main objective of the new program/project proposal could be the following: To consolidate tis...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-008-9092-1
更新日期：2009-05-01 00:00:00
abstract：:ISBT 128 is the global standard for the identification, labeling and information transfer of human blood, cell, tissue and organ products across international borders and disparate health care systems. The system has been designed and perfected over almost two decades to ensure accuracy, safety and efficiency for the ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-010-9196-2
更新日期：2010-11-01 00:00:00
abstract：:Although allografts for anterior cruciate ligament (ACL) replacement have shown advantages compared to autografts, their use is limited due to the risk of disease transmission and the limitations of available sterilization methods. Gamma sterilization has shown detrimental effects on graft properties at the high doses...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9289-6
更新日期：2012-08-01 00:00:00
abstract：:Tissue and organ transplantation is one of the most promising treatments for some incurable diseases. Nowadays, transplantation is the common therapy in many countries. Unfortunately, availability of donated tissues and organs is limited. There are several factors which may affect donation rate for instance; social fa...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9473-6
更新日期：2015-06-01 00:00:00
abstract：:Tissue engineering using human cells and tissue has one of the greatest scientific and economical potential in the coming years. There are public concerns during the ongoing discussion about future trends in life sciences and if ethic boundaries might be respected sufficiently in the course of striving for industrial ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1026392429112
更新日期：2003-01-01 00:00:00
abstract：:Delivering the promise of personalised medicine is the challenge that the current generation of scientists face. The variations in human physiology and disease are considerable, and designing appropriate strategies to deliver what has been promised will require access to tissue from a large number of volunteers. The N...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-010-9212-6
更新日期：2011-02-01 00:00:00
abstract：:The amnionic membrane is a rich source of multipotent mesenchymal stromal cells (hAMSC), which are readily available and show a potential use in regenerative medicine and tissue engineering. Before these cells can be applied clinically, careful characterization is necessary, especially as primary cells are known to ch...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-013-9415-8
更新日期：2014-06-01 00:00:00
abstract：:The study aim was to identify risk factors for morphological rejection of aortic and pulmonary valves for transplantation that could be used to optimize donor selection. The files of all Dutch heart valve donors, donating in a 2.5 years period, whose hearts were processed at Heart Valve Bank Rotterdam, were reviewed f...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9267-z
更新日期：2013-03-01 00:00:00
abstract：:This study was conducted to evaluate the effect of combined cysteine and glutathione in soy lecithin-based semen extender on post-thawed ram sperm quality. A total of 28 ejaculates were collected twice a week (from four rams) during breeding season. In each replicate, semen samples (n = 4, one ejaculate for each ram) ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9488-z
更新日期：2015-09-01 00:00:00
abstract：:We evaluated the value of the 'alternative slices mirror image method' used in prostate tissue banking in terms of predicting the sampling of cancerous tissue while preserving the pathological prognostic information. The concordance of diagnosis between banked sections and their mirror image paraffin- sections was stu...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9284-y
更新日期：2012-12-01 00:00:00
abstract：:Cellular stress to ejaculated spermatozoa such as cryopreservation is known to induce caspase-derived, apoptotic signaling. Therefore, the proenzymes and active forms of caspases 1, 3, 8 and 9 were examined by western blot technique in unfrozen and frozen human spermatozoa of infertility patients and of healthy donors...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-005-0276-7
更新日期：2006-01-01 00:00:00
abstract：:The amniotic membranes were collected from the placentae of selected and screened donors. Processing was done by washing the fresh amniotic membrane successively in sterile saline, 0.05% sodium hypochlorite solution and sterile distilled water until it was completely cleared of blood particles. The membranes were ster...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/B:CATB.0000034077.05000.29
更新日期：2004-01-01 00:00:00
abstract：:Both allogenic and autologous cultured skin cells have been used clinically on burn patients. In vitro cultivation of human keratinocytes has been routinely provided by the Central Tissue Bank in Bratislava since 1996, with an average annual production of around 7,000 cm(2). Keratinocytes have been cultivated using a ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9275-z
更新日期：2012-08-01 00:00:00
abstract：:Revision surgery of joint replacements is increasing and raises the demand for allograft bone since restoration of bone stock is crucial for longevity of implants. Proceedings of bone grafts influence the biological and mechanic properties differently. This study examines the effect of thermodisinfection on mechanic p...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-016-9567-4
更新日期：2016-09-01 00:00:00
abstract：:Living tissue engineering for regenerative therapy cannot withstand the usual pharmacopoeia methods of purification and terminal sterilization. Consequently, these products must be manufactured under aseptic conditions at microbiologically controlled environment facilities. This study was proposed to validate BacT/ALE...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9281-1
更新日期：2012-08-01 00:00:00
abstract：:Recovered human and animal tissues are used extensively in surgery for wound repair and reconstruction. In preparation for the validation of chemical disinfection and radiation sterilization processes, studies were performed on the development and validation of quantitative bioburden recovery methods for human bone an...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-010-9238-9
更新日期：2012-03-01 00:00:00
abstract：:Nowadays, there are four types of meniscal allografts known: fresh, cryopreserved, deep-frozen and lyophilized ones but only two of them are widely used in clinical practice. Use of different types of meniscal allografts still remains controversial due to preparation method, their biomechanical properties as well as c...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-013-9396-7
更新日期：2014-09-01 00:00:00
abstract：:Recent studies have reported that stem cells can be isolated from a wide range of tissues including bone marrow, fatty tissue, adipose tissue and placenta. Moreover, several studies also suggest that skin dermis could serve as a source of stem cells, but are of unclear phenotype. Therefore, we isolated and investigate...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9471-8
更新日期：2015-06-01 00:00:00
abstract：:The aim of the present study was to compare the effects of two freezing methods, vapor phase and very rapid freezing, with and without cryoprotectant on semen parameters in men with normal semen criteria. Cryopreservation was done on semen samples from 31 men by two methods of vapor phase freezing and very rapid freez...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-012-9351-z
更新日期：2013-12-01 00:00:00
abstract：:The aim of this study is to evaluate the beneficial effect of Myoinositol (MYO) supplement in freezing media on the post thaw sperm quality. Semen samples from 40 normozoospermic men were divided into two aliquots and frozen with simple or 2 mg/mL MYO supplemented freezing medium. Post thaw process including, computer...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-018-9731-0
更新日期：2019-03-01 00:00:00
abstract：:Decidual stromal cells (DSCs) from maternal term placenta represent a potential source of cells for the treatment of cardiovascular and graft-versus-host diseases. However, it is not clear whether DSCs could be induced towards cardiomyocyte-like differentiation. We chose the placentas which should bred male new-baby. ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9493-x
更新日期：2015-09-01 00:00:00
