Abstract：
:This study aimed to optimise methods of cryopreserving human articular cartilage (AC) tissue for the isolation of late chondrocytes. Human AC specimens from osteoarthritis patients who had undergone total knee replacement were used to optimise the chondrocyte isolation process and the choice of cryoprotective agent (CPA). For AC tissue cryopreservation, intact cored cartilage discs (5 mm diameter) and diced cartilage (0.2-1 mm cubes) from the same sized discs were step cooled and stored in liquid nitrogen for up to 48 h before chondrocyte isolation and in vitro assay of cell viability and proliferative potential. The results showed that 10 % dimethyl sulphoxide in 90 % foetal bovine serum was a successful CPA for chondrocyte cryopreservation. Compared with intact cored discs, dicing of AC tissue into 0.2-1 mm cubes significantly increased the viability and proliferative capacity of surviving chondrocytes after cryopreservation. In situ cross-section imaging using focused ion beam microscopy revealed that dicing of cored AC discs into small cubes reduced the cryo-damage to cartilage tissue matrix. In conclusion, modification of appropriate factors, such as the size of the tissue, cryoprotective agent, and isolation protocol, can allow successful isolation of viable chondrocytes with high proliferative capacity from cryopreserved human articular cartilage tissue. Further studies are required to determine whether these cells may retain cartilage differentiation capacity and provide sufficient chondrocytes for use as implants in clinical applications.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Xia Z,Duan X,Murray D,Triffitt JT,Price AJdoi
10.1007/s10561-012-9328-ysubject
Has Abstractpub_date
2013-06-01 00:00:00pages
267-76issue
2eissn
1389-9333issn
1573-6814journal_volume
14pub_type
杂志文章abstract：:Most protocols to cryopreserve ovarian tissue utilize the permeable cryoprotective agents (CPAs) in 1.5 M concentration. However the issues related to the ability to use higher concentrations of CPAs have remained open. The research aim was to assess the efficiency of media containing osmotically active sugars (sucros...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9623-8
更新日期：2018-03-01 00:00:00
abstract：:Revision surgery of joint replacements is increasing and raises the demand for allograft bone since restoration of bone stock is crucial for longevity of implants. Proceedings of bone grafts influence the biological and mechanic properties differently. This study examines the effect of thermodisinfection on mechanic p...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-016-9567-4
更新日期：2016-09-01 00:00:00
abstract：:The aim of the study was to determine the relationship between the maternal age at delivery and selected properties of the cord blood stem cells. The study included 50 pregnant women aged between 18 and 38 years in which spontaneous labors or elective cesarean sections were performed. Umbilical cord blood was collecte...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9657-y
更新日期：2018-03-01 00:00:00
abstract：:Decidual stromal cells (DSCs) from maternal term placenta represent a potential source of cells for the treatment of cardiovascular and graft-versus-host diseases. However, it is not clear whether DSCs could be induced towards cardiomyocyte-like differentiation. We chose the placentas which should bred male new-baby. ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9493-x
更新日期：2015-09-01 00:00:00
abstract：BACKGROUND:The role of bone and tissue banking is well known to meet the ever-growing need of bone and soft tissue allografts. Strict guidelines have been established to ensure high standard and minimize complications related to bone transplantation. METHODS:The Bone Bank in King Faisal Specialist Hospital and Researc...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09868-7
更新日期：2020-10-04 00:00:00
abstract：:Decellularised tissue allografts have been used in reconstructive surgical applications and transplantation for many years. Some of the current methods of sterilisation have a detrimental effect on the tissue graft structure and function. The anti-microbial activity of cupric ions and hydrogen peroxide (H2O2) are well...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9660-3
更新日期：2017-12-01 00:00:00
abstract：:Nowadays, there are four types of meniscal allografts known: fresh, cryopreserved, deep-frozen and lyophilized ones but only two of them are widely used in clinical practice. Use of different types of meniscal allografts still remains controversial due to preparation method, their biomechanical properties as well as c...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-013-9396-7
更新日期：2014-09-01 00:00:00
abstract：:Human amniotic membrane (hAM) represents a tissue that is well established as biomaterial in the clinics with potential for new applications in regenerative medicine. For tissue engineering (TE) strategies, cells are usually combined with inductive factors and a carrier substrate. We have previously recognized that hA...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9454-9
更新日期：2014-06-01 00:00:00
abstract：:The Central Tissue Bank in Warsaw was established in 1963 and since then ionising radiation has been routinely applied to sterilise tissue grafts. Connective tissue grafts such as bone, cartilage, tendons, sclera, pericardium, skin, acellular dermis and amnion irradiated with a dose of 35 kGy in a (60)Co source and/or...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-005-0338-x
更新日期：2005-01-01 00:00:00
abstract：:Recently Bulgarian Bone Marrow Donors Registry (BBMDR) has been established and since August 2005 it has been a member of Bone Marrow Donors Worldwide. Currently the number of healthy donors included in the BBMDR is relatively low. All donors included in the BBMDR are typed for HLA-A, -B, -DRB loci. Phylogenetic analy...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-007-9046-z
更新日期：2008-12-01 00:00:00
abstract：:The aim of this paper was to evaluate through histological analysis of the tissue reaction of deproteinized bovine bone matrix (DBBM) when inserted into the site of intramuscular ectopic sheep. In this study, 16 sheep received 3 groups and these were divided into 2 experimental times: Group 1-sham group, Group 2-parti...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-016-9588-z
更新日期：2016-12-01 00:00:00
abstract：:This is a report of a workshop held on the establishment of human research tissue banking which was held in Levi, Finland 21-24 March 2002. There were 21 participants from 7 European countries. This meeting was attended by representatives from academia, research tissue banks and from the Biotech and Pharmaceutical Ind...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1022811232250
更新日期：2002-01-01 00:00:00
abstract：:Musculo-skeletal allografts sterilized and deep frozen are among the most common human tissue to be preserved and utilized in modern medicine. The effects of a long deep freezing period on cortical bone has already been evaluated and found to be insignificant. However, there are no reports about the influences of a pr...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-008-9106-z
更新日期：2009-02-01 00:00:00
abstract：:In breast cancer, various clinical parameters are assessed to define clinical stage and thus obtain a more accurate prognosis. However, banks of tumor tissues are an important source of material for studies of risk of recurrence and of features governing clinical outcome in breast cancer. Although the heterogeneous ch...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-009-9124-5
更新日期：2009-11-01 00:00:00
abstract：:Both allogenic and autologous cultured skin cells have been used clinically on burn patients. In vitro cultivation of human keratinocytes has been routinely provided by the Central Tissue Bank in Bratislava since 1996, with an average annual production of around 7,000 cm(2). Keratinocytes have been cultivated using a ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9275-z
更新日期：2012-08-01 00:00:00
abstract：:The use of glycosaminoglycans (GAGs) as the second macromolecular component with collagen for the membrane preparation is based on the idea to create a biodegradable scaffold for new tissue. A hybrid collagen/hyaluronan membrane with specific bubble macrostructure was designed to serve as a synthetic dermal substitute...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/a:1010181925236
更新日期：2000-01-01 00:00:00
abstract：:ISBT 128 is the global standard for the identification, labeling and information transfer of human blood, cell, tissue and organ products across international borders and disparate health care systems. The system has been designed and perfected over almost two decades to ensure accuracy, safety and efficiency for the ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-010-9196-2
更新日期：2010-11-01 00:00:00
abstract：:Although allografts for anterior cruciate ligament (ACL) replacement have shown advantages compared to autografts, their use is limited due to the risk of disease transmission and the limitations of available sterilization methods. Gamma sterilization has shown detrimental effects on graft properties at the high doses...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9289-6
更新日期：2012-08-01 00:00:00
abstract：:Insight into the pathogenesis of neurodegenerative disorders requires accurately categorized postmortem human brain tissue. This article introduces electronic tissue tracking and management as implemented at New York Brain Bank (NYBB) through processing of the brain at fresh state and storing standardized frozen sampl...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-008-9078-z
更新日期：2008-09-01 00:00:00
abstract：:Injured nerves cannot regenerate on their own, and a lack of engraftable human nerves has been a major obstacle in cell-based therapies for regenerating damaged nerves. A monolayer culture approach to obtain adherent neural stem cells from human embryonic stem cells (hESC-NSCs) was established, and the greatest number...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09816-5
更新日期：2020-06-01 00:00:00
abstract：:To vitrify human amniotic membrane specimens so that the maximum of epithelial cells survives in order to develop a procedure for the eventual vitrification of posterior corneal lamellae without using cryoprotective agents. To assess different methods of tissue sample preparation preceding vitrification. In group 1, t...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-013-9388-7
更新日期：2014-03-01 00:00:00
abstract：:Rheumatoid arthritis (RA) is the most common degenerative arthritic cartilage and represents a disease where the prospect of stem cell therapy offers considerable hope. Currently, bone marrow (BM) represents the major source of mesenchymal stem cells (MSCs) for cell therapy. In the pathology of RA, the pro-inflammator...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-012-9354-9
更新日期：2013-12-01 00:00:00
abstract：:Cellular stress to ejaculated spermatozoa such as cryopreservation is known to induce caspase-derived, apoptotic signaling. Therefore, the proenzymes and active forms of caspases 1, 3, 8 and 9 were examined by western blot technique in unfrozen and frozen human spermatozoa of infertility patients and of healthy donors...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-005-0276-7
更新日期：2006-01-01 00:00:00
abstract：:Tissue engineering using human cells and tissue has one of the greatest scientific and economical potential in the coming years. There are public concerns during the ongoing discussion about future trends in life sciences and if ethic boundaries might be respected sufficiently in the course of striving for industrial ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1026392429112
更新日期：2003-01-01 00:00:00
abstract：:On March 19 World Health Organization declare the pandemic situation by outbreak coronavirus disease 2019 in the world. The pressure on the health care system has been very high in several countries. Spanish National Transplant Organization (ONT) have made many efforts in maintaining transplantation activity. Although...
journal_title：Cell and tissue banking
pub_type： 杂志文章,多中心研究
doi：10.1007/s10561-020-09853-0
更新日期：2020-12-01 00:00:00
abstract：:We describe a rapid and easy technique for the preparation of pre-stripped Descemet membrane (DM) endothelial keratoplasty (DMEK) tissue in the eye bank, without the use of dye or trephining by the eye bank technician. After drawing 2 radial marks 90° apart on the scleral rim of the cornea, a circumferential incision ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-019-09771-w
更新日期：2019-06-01 00:00:00
abstract：:The regulatory framework of tissue banking introduces a number of requirements for monitoring cleanrooms for processing tissue or cell grafts. Although a number of requirements were clearly defined, some requirements are open for interpretation. This study aims to contribute to the interpretation of GMP or GTP guideli...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-012-9355-8
更新日期：2013-12-01 00:00:00
abstract：:Human epidermal melanocytes can be induced to form melanocyte spheroids and revert to immature characteristics by long-term trypsinization (LTT). To further explore the biological characteristics of melanocytes after LTT and to study the underlying mechanism. Melanocytes were subjected to long-term (2 h) trypsinizatio...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09866-9
更新日期：2020-09-25 00:00:00
abstract：:Autologous chondrocyte implantation has been shown to be a promising method for treatment of deep articular cartilage defects. The hyaline cartilage formed by implanted autologous chondrocytes has biomechanical properties similar to those of natural articular cartilage. Between June 2006 and September 2008 we performe...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-009-9163-y
更新日期：2010-05-01 00:00:00
abstract：:We report two cases of severe endophthalmitis, which were caused by Serratia marcescens, and developed in the immediate postoperative period in two recipients of corneal grafts from the same donor. The cause of the donor's death was massive CVA. He had been on mechanical ventilation for 12 days before he died, and had...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1021839711471
更新日期：2002-01-01 00:00:00
