Abstract:
:Human skin allografts can be preserved by different methods. In our clinical practice, human skin allografts are harvested on multi-organ and tissue donors, transferred at +4°C in Ringer Lactate, cryopreserved with 15% Glycerol and held in the vapor phase of a liquid nitrogen freezer until delivery to the burn center. The aim of this experimental study was to evaluate the impact of transport medium and cryoprotectant on the viability of human skin allografts. For this purpose, we compared skin samples harvested from 19 multi-organ and tissue donors with two different transport media and two different cryoprotectants. Viability was assessed by the MTT assay after harvesting at laboratory reception, during storage (at +4°C) at day 2 and day 7, and after cryopreservation and thawing. Histopathological analysis was performed for each MTT assay. Results indicate that, when stored at +4°C, skin retains more viability with RPMI, whereas Glycerol and DMSO are equivalent cryoprotectants regardless of the transport medium. In conclusion, our protocol could be improved by the utilization of RPMI as transport medium.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Gaucher S,Elie C,Vérola O,Jarraya Mdoi
10.1007/s10561-011-9239-3subject
Has Abstractpub_date
2012-03-01 00:00:00pages
147-55issue
1eissn
1389-9333issn
1573-6814journal_volume
13pub_type
杂志文章abstract::In this study, the efficiency of the "Needle Immersed Vitrification" technique was tested on cryopreserved feline ovarian tissue. For vitrification, ovarian fragments (0.5-1.5 mm2) from each ovary were collected; the grafts were exposed to 7.5-15% ethylene glycol and 7.5-15% dimethyl sulfoxide at room temperature and ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-017-9663-0
更新日期:2018-03-01 00:00:00
abstract::Rheumatoid arthritis (RA) is the most common degenerative arthritic cartilage and represents a disease where the prospect of stem cell therapy offers considerable hope. Currently, bone marrow (BM) represents the major source of mesenchymal stem cells (MSCs) for cell therapy. In the pathology of RA, the pro-inflammator...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9354-9
更新日期:2013-12-01 00:00:00
abstract::Recovered human and animal tissues are used extensively in surgery for wound repair and reconstruction. In preparation for the validation of chemical disinfection and radiation sterilization processes, studies were performed on the development and validation of quantitative bioburden recovery methods for human bone an...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-010-9238-9
更新日期:2012-03-01 00:00:00
abstract::Terminal sterilization of musculoskeletal allografts by gamma radiation minimizes the risk of disease transmission but impairs allograft mechanical properties. Commonly employed crosslinking agents can sterilize tissues without affecting mechanical properties adversely; however, these agents are toxic. Genipin is repo...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9335-z
更新日期:2013-09-01 00:00:00
abstract:BACKGROUND:Despite improved surgical techniques and the use of new medication, healing of corneal and conjunctival defects cannot always be achieved. In this connection the clinical use of human amnion, produced by different techniques, has represented a successful alternative for the past ten years. The purpose of the...
journal_title:Cell and tissue banking
pub_type: 临床试验,杂志文章
doi:10.1023/b:catb.0000022222.41304.de
更新日期:2004-01-01 00:00:00
abstract::Our Department of Plastic and Reconstructive Surgery has routinely been using amnion preserved in glycerol for the treatment of debrided II degrees burns. This treatment is almost pain free and requires fewer changes of dressings and fewer anaesthetics. It also prevents overgrowing granulation tissue and lessens scarr...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/A:1014316232009
更新日期:2001-01-01 00:00:00
abstract::This study was conducted to compare the quantitative and qualitative indices of the donated corneas maintained in either Optisol-GS or Eusol-C storage media. In an ante-grade single blind study, two corneas from each donor with a death to preservation time of less than 30 h and with a minimum of "an apparent good corn...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-014-9466-5
更新日期:2015-06-01 00:00:00
abstract::Autologous chondrocyte implantation has been shown to be a promising method for treatment of deep articular cartilage defects. The hyaline cartilage formed by implanted autologous chondrocytes has biomechanical properties similar to those of natural articular cartilage. Between June 2006 and September 2008 we performe...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-009-9163-y
更新日期:2010-05-01 00:00:00
abstract::Homograft or allograft tissue has been available for use as replacement for diseased valves or reconstruction of major vessels for decades. However, with respect to replacement of diseased valvular tissue the search for the ideal valve still continues. In this review we will discuss the clinical indications, surgical ...
journal_title:Cell and tissue banking
pub_type: 杂志文章,评审
doi:10.1007/s10561-006-9009-9
更新日期:2006-01-01 00:00:00
abstract::As banked human tissues are not widely available, the development of new non-destructive and contactless techniques to evaluate the quality of allografts before distribution for transplantation is very important. Also, tissues will be processed accordingly to standard procedures and to minimize disease transmission mo...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-013-9389-6
更新日期:2014-09-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) are used for tissue regeneration in several pathological conditions, including autoimmune diseases. However, the optimal sources and culture requirements for these cells are still under investigation. Here, we compared mRNA expression in dermal MSCs (DMSCs) at passage (P) 3 and P5 to prov...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-017-9672-z
更新日期:2018-09-01 00:00:00
abstract::Cerebral ischemic injury as the main manifestation of stroke can occur in stroke patients (70-80%). Nowadays, the main therapeutic strategy used for ischemic brain injury treatment aims to achieve reperfusion, neuroprotection, and neurorecovery. Also, angiogenesis as a therapeutic approach maybe represents a promising...
journal_title:Cell and tissue banking
pub_type: 杂志文章,评审
doi:10.1007/s10561-020-09885-6
更新日期:2020-11-24 00:00:00
abstract::Increasingly dental surgeons face the challenge of reconstruction of the height and/or thickness of the alveolar ridge as more and more patients wish to have permanent restoration of their dental defects based on intraosseous implants. Evaluation of human allogeneic bone tissue grafts in reconstruction of atrophied al...
journal_title:Cell and tissue banking
pub_type: 临床试验,杂志文章
doi:10.1007/s10561-015-9512-y
更新日期:2015-12-01 00:00:00
abstract::Although therapeutics derived from biological sources have been subjected to regulatory oversight for some time, the products used in transplantation procedures have historically been exempt from this oversight. These products have been viewed as being part of medical practice rather than as the result of mainstream p...
journal_title:Cell and tissue banking
pub_type: 杂志文章,评审
doi:10.1007/s10561-006-9012-1
更新日期:2006-01-01 00:00:00
abstract::Since there is no upper age limit for general organ donation, unlike heart valve donation, and since a quarter of all organ donors are 65 years and older, we examined whether the heart valves from these donors are suitable as allografts. In the period 1999-2004 the aortic valve and pulmonary valve of 100 organ donors ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-007-9052-1
更新日期:2008-03-01 00:00:00
abstract::Demineralized freeze-dried bone allograft (DFDBA) has been used extensively in periodontal therapy. Questions have been raised however, about the osteogenic potential of the variety of grafts available. In India the cost factor is another important consideration. The aim of this study therefore was to evaluate the cli...
journal_title:Cell and tissue banking
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10561-014-9481-6
更新日期:2015-09-01 00:00:00
abstract::The Central Tissue Bank in Warsaw was established in 1963 and since then ionising radiation has been routinely applied to sterilise tissue grafts. Connective tissue grafts such as bone, cartilage, tendons, sclera, pericardium, skin, acellular dermis and amnion irradiated with a dose of 35 kGy in a (60)Co source and/or...
journal_title:Cell and tissue banking
pub_type: 杂志文章,评审
doi:10.1007/s10561-005-0338-x
更新日期:2005-01-01 00:00:00
abstract::Outcomes for patients with metastatic and recurrent osteosarcoma remain poor and a better understanding of the biology of this malignancy is critical to the development of prognostic biomarkers and novel therapies. The purpose of this study was to establish a biobank of osteosarcoma which has the potential of monitori...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-020-09831-6
更新日期:2020-09-01 00:00:00
abstract::The contamination of the transport solution used in cardiovascular allografts can occur from different sources. Risk factors associated with positive microbiological test of transport solution have not been reported previously. This study aimed to determine the risk factor for contamination of transport solution used ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-019-09754-x
更新日期:2019-03-01 00:00:00
abstract::To vitrify human amniotic membrane specimens so that the maximum of epithelial cells survives in order to develop a procedure for the eventual vitrification of posterior corneal lamellae without using cryoprotective agents. To assess different methods of tissue sample preparation preceding vitrification. In group 1, t...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-013-9388-7
更新日期:2014-03-01 00:00:00
abstract::Residual chemicals that are presented during tissue processing in human tissue banks can be a risk for the allograft recipient. Determine the residual concentrations of the antibiotics and detergent used in the process of human decellularized tissue-engineered heart valves stored in isotonic saline solution up to 18 m...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-020-09856-x
更新日期:2020-12-01 00:00:00
abstract::Cell manufacturing for clinical applications is a unique form of biologics manufacturing that relies on maintenance of stringent work practices designed to ensure product consistency and prevent contamination by microorganisms or by another patient's cells. More extensive, prolonged laboratory processes involve greate...
journal_title:Cell and tissue banking
pub_type: 杂志文章,评审
doi:10.1007/s10561-011-9276-y
更新日期:2012-12-01 00:00:00
abstract::Cellular stress to ejaculated spermatozoa such as cryopreservation is known to induce caspase-derived, apoptotic signaling. Therefore, the proenzymes and active forms of caspases 1, 3, 8 and 9 were examined by western blot technique in unfrozen and frozen human spermatozoa of infertility patients and of healthy donors...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-005-0276-7
更新日期:2006-01-01 00:00:00
abstract::Human sperm cryopreservation is a common technique which is used in assisted reproductive technologies. Despite the existence of evidence supporting the production of ROS and DNA fragmentation during sperm cryopreservation, there is little and equivocal information about the cryopreservation effects on methylation of ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-020-09828-1
更新日期:2020-09-01 00:00:00
abstract::Freeze-dried dura mater allograft (FDDMA) was evaluated as a cost-effective collagenous barrier using an experimental animal model to determine its biocompatibility and 'cell-occlusivity' potential. The FDDMA was processed at the Civil Hospital Ahmedabad, in accordance with the guidelines provided by the South-Eastern...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/B:CATB.0000007039.98845.bd
更新日期:2003-01-01 00:00:00
abstract::Islet transplantation has made major progress to treat patients with type 1 diabetes. Islet mass and quality are critically important to ensure successful transplantation. Currently, islet status is evaluated using insulin secretion, oxygen consumption rate, or adenosine triphosphate (ATP) measurement. These parameter...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-017-9659-9
更新日期:2018-03-01 00:00:00
abstract::We evaluated the value of the 'alternative slices mirror image method' used in prostate tissue banking in terms of predicting the sampling of cancerous tissue while preserving the pathological prognostic information. The concordance of diagnosis between banked sections and their mirror image paraffin- sections was stu...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-011-9284-y
更新日期:2012-12-01 00:00:00
abstract::In order to guarantee the required level of quality for our Bone &Tissue Banking, we evaluated a new CE marked container (CELLFLEX MacoPharma), for packing, transport, processing and storage of bones for therapeutic use. The use of CE marked containers is mandatory for organ and tissue containers (Medical Device Direc...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-004-1090-3
更新日期:2004-01-01 00:00:00
abstract::In Malaysia, tissue banking activities began in Universiti Sains Malaysia (USM) Tissue Bank in early 1990s. Since then a few other bone banks have been set up in other government hospitals and institutions. However, these banks are not governed by the national authority. In addition there is no requirement set by the ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-010-9188-2
更新日期:2010-11-01 00:00:00
abstract::Decidual stromal cells (DSCs) from maternal term placenta represent a potential source of cells for the treatment of cardiovascular and graft-versus-host diseases. However, it is not clear whether DSCs could be induced towards cardiomyocyte-like differentiation. We chose the placentas which should bred male new-baby. ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-015-9493-x
更新日期:2015-09-01 00:00:00