Abstract:
:The amnionic membrane is a rich source of multipotent mesenchymal stromal cells (hAMSC), which are readily available and show a potential use in regenerative medicine and tissue engineering. Before these cells can be applied clinically, careful characterization is necessary, especially as primary cells are known to change their phenotype in culture. We analyzed the mesenchymal phenotype of hAMSC at different stages after isolation using immunohistochemistry. Shortly after isolation (1 day), 92 % (± 7 %) of the hAMSC expressed the mesenchymal marker vimentin, 2 % (± 1 %) stained for the epithelial marker cytokeratin-7 and 5 % (± 4 %) co-expressed these markers. After 5 days, the double positive cells slightly increased to 7 % (± 3 %), while exclusive expression of cytokeratin-7 or vimentin remained unchanged (1 % ± 2 % and 92 % ± 1 %, respectively). After the first passage, all attached cells were vimentin-positive, while 54 % (± 9 %) co-expressed cytokeratin-7 and vimentin. Thus, we conclude that under culture, hAMSC adopt a hybrid mesenchymal-epithelial phenotype. It is also essential to perform microscopical examination during the first days after isolation to detect contaminations with human amnion-derived epithelial cells in cultures of hAMSC.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
König J,Lang I,Siwetz M,Fröhlich J,Huppertz Bdoi
10.1007/s10561-013-9415-8subject
Has Abstractpub_date
2014-06-01 00:00:00pages
193-8issue
2eissn
1389-9333issn
1573-6814journal_volume
15pub_type
杂志文章abstract::Recovered human and animal tissues are used extensively in surgery for wound repair and reconstruction. In preparation for the validation of chemical disinfection and radiation sterilization processes, studies were performed on the development and validation of quantitative bioburden recovery methods for human bone an...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-010-9238-9
更新日期:2012-03-01 00:00:00
abstract::Perivascular cells are known to be ancestors of mesenchymal stem cells (MSCs) and can be obtained from heart, skin, bone marrow, eye, placenta and umbilical cord (UC). However detailed characterization of perivascular cells around the human UC vein and comparative analysis of them with MSCs haven't been done yet. In t...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-015-9542-5
更新日期:2016-06-01 00:00:00
abstract::A thick gingival biotype is a requisite for good periodontal health. It has important role in resisting trauma and subsequent gingival recession. The gingival thickness is a significant predictor of clinical outcome of periodontal surgeries. Various surgical procedures are used to increase the gingival thickness. The ...
journal_title:Cell and tissue banking
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10561-015-9524-7
更新日期:2016-03-01 00:00:00
abstract::In India, the procurement of tissues for transplantation is governed by the Transplantation of Human Organs Act, 1994. However, although this law exists, it is primarily applied to organ transplantation and rules and regulations that are specific to tissue banking have yet to be developed.The Tata Memorial Hospital (T...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/B:CATB.0000007026.00604.97
更新日期:2003-01-01 00:00:00
abstract::Increasingly dental surgeons face the challenge of reconstruction of the height and/or thickness of the alveolar ridge as more and more patients wish to have permanent restoration of their dental defects based on intraosseous implants. Evaluation of human allogeneic bone tissue grafts in reconstruction of atrophied al...
journal_title:Cell and tissue banking
pub_type: 临床试验,杂志文章
doi:10.1007/s10561-015-9512-y
更新日期:2015-12-01 00:00:00
abstract::ISBT 128 is the global standard for the identification, labeling and information transfer of human blood, cell, tissue and organ products across international borders and disparate health care systems. The system has been designed and perfected over almost two decades to ensure accuracy, safety and efficiency for the ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-010-9196-2
更新日期:2010-11-01 00:00:00
abstract::In order to guarantee the required level of quality for our Bone &Tissue Banking, we evaluated a new CE marked container (CELLFLEX MacoPharma), for packing, transport, processing and storage of bones for therapeutic use. The use of CE marked containers is mandatory for organ and tissue containers (Medical Device Direc...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-004-1090-3
更新日期:2004-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) though multipotent exhibit limited lifespan in vitro, with progressive reduction in capacity for self-renewal leading to irreversible arrest of cell division, which limits their use for therapeutic purposes. Human umbilical cord wall MSCs are easy to process and proliferate rapidly in cul...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-015-9525-6
更新日期:2016-03-01 00:00:00
abstract::To vitrify human amniotic membrane specimens so that the maximum of epithelial cells survives in order to develop a procedure for the eventual vitrification of posterior corneal lamellae without using cryoprotective agents. To assess different methods of tissue sample preparation preceding vitrification. In group 1, t...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-013-9388-7
更新日期:2014-03-01 00:00:00
abstract::We evaluated the value of the 'alternative slices mirror image method' used in prostate tissue banking in terms of predicting the sampling of cancerous tissue while preserving the pathological prognostic information. The concordance of diagnosis between banked sections and their mirror image paraffin- sections was stu...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-011-9284-y
更新日期:2012-12-01 00:00:00
abstract::Allogeneic mandibular bone processed by the deep frozen method was used as a biologic crib for mandibular reconstruction. The allogeneic mandible is biocompatible, bioresorbable, of low antigenicity and provides the morphology for symphysis contour, angle of mandible and dental arch form. The particulate cancellous bo...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/A:1023602717328
更新日期:2002-01-01 00:00:00
abstract::Homograft or allograft tissue has been available for use as replacement for diseased valves or reconstruction of major vessels for decades. However, with respect to replacement of diseased valvular tissue the search for the ideal valve still continues. In this review we will discuss the clinical indications, surgical ...
journal_title:Cell and tissue banking
pub_type: 杂志文章,评审
doi:10.1007/s10561-006-9009-9
更新日期:2006-01-01 00:00:00
abstract::Human sperm banking is an important procedure in the assisted reproductive technique centers. It entails sperm damage. The aim of this study was to investigate beneficial effect of Ceratonia siliqua (C. siliqua) supplement in freezing/thawing media on post thaw sperm parameters and sperm chromatin quality in normozoos...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-019-09779-2
更新日期:2019-09-01 00:00:00
abstract::Human skin allografts are one of the best temporary biological coverings for severely burned patients. Cryopreserved (CPA) and glycerol-preserved (GPA) allografts are the most widely used types. This study compared the allograft efficiency of both preservation methods under the same conditions. To simulate actual clin...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-015-9521-x
更新日期:2016-03-01 00:00:00
abstract::The goal of periodontal therapy is to prevent periodontal disease progression and to regenerate lost periodontal tissues. Various bone grafts are used alone or in combination with other treatment modalities for periodontal therapy. In literature no reports are presented with block allografts in the treatment of intrab...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-018-09744-5
更新日期:2019-02-11 00:00:00
abstract::The regulatory framework of tissue banking introduces a number of requirements for monitoring cleanrooms for processing tissue or cell grafts. Although a number of requirements were clearly defined, some requirements are open for interpretation. This study aims to contribute to the interpretation of GMP or GTP guideli...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9355-8
更新日期:2013-12-01 00:00:00
abstract::Islet transplantation has made major progress to treat patients with type 1 diabetes. Islet mass and quality are critically important to ensure successful transplantation. Currently, islet status is evaluated using insulin secretion, oxygen consumption rate, or adenosine triphosphate (ATP) measurement. These parameter...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-017-9659-9
更新日期:2018-03-01 00:00:00
abstract::Tissue banking is started in Thailand in 1979 at the Department of Orthopaedic Surgery, Siriraj Hospital, Bangkok. At that time tissues produced were freeze-dried bone allografts which were sterilized by ethylene oxide. In 1984, the freeze-dried tissue allograft project received an award from the National Research Cou...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/A:1026542730541
更新日期:2000-01-01 00:00:00
abstract::Good manufacturing practices guidelines require safer and standardized cell substrates especially for those cell therapy products to treat ocular diseases where fibroblasts are used as feeder layers. However, if these are unavailable for stem cells culturing, murine fibroblasts are regularly used, raising critical iss...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9325-1
更新日期:2013-06-01 00:00:00
abstract::Since there is no upper age limit for general organ donation, unlike heart valve donation, and since a quarter of all organ donors are 65 years and older, we examined whether the heart valves from these donors are suitable as allografts. In the period 1999-2004 the aortic valve and pulmonary valve of 100 organ donors ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-007-9052-1
更新日期:2008-03-01 00:00:00
abstract::A limited number of grafts produced in one batch is the main constrain to validate radiation sterilization dose of amnion and bone grafts according to ISO standard. The validation experiments done were according to ISO 13409 with a slight modification in sampling method. The experiments were carried out three times by...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/A:1010129720363
更新日期:2000-01-01 00:00:00
abstract::Both allogenic and autologous cultured skin cells have been used clinically on burn patients. In vitro cultivation of human keratinocytes has been routinely provided by the Central Tissue Bank in Bratislava since 1996, with an average annual production of around 7,000 cm(2). Keratinocytes have been cultivated using a ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-011-9275-z
更新日期:2012-08-01 00:00:00
abstract::Brain donations are imperative for research; understanding possible barriers to entry is required to improve brain donation rates. While a few surveys have studied attitudes towards brain banking in patients with neurodegenerative disorders, none have surveyed patients with chronic neurological disorders but without n...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-020-09819-2
更新日期:2020-06-01 00:00:00
abstract::It has been predicted that one of the greatest increase in prevalence of diabetes will happen in the Middle East bear in the next decades. The aim of standard therapeutic strategies for diabetes is better control of complications. In contrast, some new strategies like cell and gene therapy have aimed to cure the disea...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-011-9273-1
更新日期:2012-12-01 00:00:00
abstract::To compare the quality of the repair tissue in three-dimensional co-culture of human chondrocytes implanted in an in vivo model. Six cadaveric and five live human donors were included. Osteochondral biopsies from the donor knees were harvested for chondrocyte isolation. Fifty percent of cadaveric chondrocytes were exp...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-017-9635-4
更新日期:2017-09-01 00:00:00
abstract::Human adipose-derived stem cells (hADSCs) have the ability to influence immune response, and hence are key cell sources for tissue repair and regeneration. In this study we explored the effect of continuous passage on the immunomodulatory properties of hADSCs to provide some advises for large-scale production of hADSC...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-014-9451-z
更新日期:2015-03-01 00:00:00
abstract:BACKGROUND:The role of bone and tissue banking is well known to meet the ever-growing need of bone and soft tissue allografts. Strict guidelines have been established to ensure high standard and minimize complications related to bone transplantation. METHODS:The Bone Bank in King Faisal Specialist Hospital and Researc...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-020-09868-7
更新日期:2020-10-04 00:00:00
abstract::The Central Tissue Bank in Warsaw was established in 1963 and since then ionising radiation has been routinely applied to sterilise tissue grafts. Connective tissue grafts such as bone, cartilage, tendons, sclera, pericardium, skin, acellular dermis and amnion irradiated with a dose of 35 kGy in a (60)Co source and/or...
journal_title:Cell and tissue banking
pub_type: 杂志文章,评审
doi:10.1007/s10561-005-0338-x
更新日期:2005-01-01 00:00:00
abstract::The purpose of the present research was: 1. To investigate the opportunity of application of allogen osteoplastic materials such as Lioplast, received in Samara Tissue Bank of Samara State Medical University. 2. To work out a new technique of producing lyophilized allogen osteoplastic materials, such as Lioplast, with...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-004-7254-3
更新日期:2005-01-01 00:00:00
abstract::Demineralized freeze-dried bone allograft (DFDBA) has been used extensively in periodontal therapy. Questions have been raised however, about the osteogenic potential of the variety of grafts available. In India the cost factor is another important consideration. The aim of this study therefore was to evaluate the cli...
journal_title:Cell and tissue banking
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10561-014-9481-6
更新日期:2015-09-01 00:00:00