Abstract：
:Human skin allografts are one of the best temporary biological coverings for severely burned patients. Cryopreserved (CPA) and glycerol-preserved (GPA) allografts are the most widely used types. This study compared the allograft efficiency of both preservation methods under the same conditions. To simulate actual clinical conditions, we used a porcine wound model. In addition, we evaluated the macroscopic and microscopic scoring of graft performance for each method. Porcine cadaver skin 1 mm thick was obtained from one pig. Cryopreserved skin cell viability was 20.8 %, glycerol-preserved skin was 9.08 %, and fresh skin was 58.6 %. We made ten partial-thickness wounds each in two pigs. The take rates on day 2 were 96.23 and 82.65 % in the GPA and CPA group (both n = 9), respectively. After 1 week, the take rates of both groups were nearly equal. The removal rate at week 5 was 98.87 and 94.41 % in the GPA and CPA group, respectively. On microscopic findings at week 2, inflammation was greater in the CPA group. Other findings such as fibroblast hyperplasia and neovascularization were not significantly different between both groups. At week 5, the score of collagen fiber synthesis was 2.67 ± 0.47 and 2.33 ± 0.47 in the GPA and CPA group, respectively. The epidermal-dermal junction was 2.22 ± 0.79 and 2.00 ± 0.47 in the GPA and CPA group, respectively. These findings suggest that wound healing takes longer in the CPA group. The preservation method of allografts is not a absolute factor in the wound healing process in this wound model.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Yoon C,Lim K,Lee S,Choi Y,Choi Y,Lee Jdoi
10.1007/s10561-015-9521-xsubject
Has Abstractpub_date
2016-03-01 00:00:00pages
21-31issue
1eissn
1389-9333issn
1573-6814pii
10.1007/s10561-015-9521-xjournal_volume
17pub_type
杂志文章abstract：:As banked human tissues are not widely available, the development of new non-destructive and contactless techniques to evaluate the quality of allografts before distribution for transplantation is very important. Also, tissues will be processed accordingly to standard procedures and to minimize disease transmission mo...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-013-9389-6
更新日期：2014-09-01 00:00:00
abstract：:This study was conducted to compare the quantitative and qualitative indices of the donated corneas maintained in either Optisol-GS or Eusol-C storage media. In an ante-grade single blind study, two corneas from each donor with a death to preservation time of less than 30 h and with a minimum of "an apparent good corn...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9466-5
更新日期：2015-06-01 00:00:00
abstract：:The Israel National Skin Bank (INSB) was founded jointly by the Israel Defense Forces (IDF) Medical Corps and the Ministry of Health in 1986. The prime purpose of the Skin Bank is to treat burn victims incurred at war or during mass casualty incidences. The INSB Protocol is comprised of international skin bank protoco...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1010183719662
更新日期：2000-01-01 00:00:00
abstract：:Malaria is one of the exclusion criteria used in selecting tissue donors and the absence of this information can lead to rejection of tissues for transplant. The studies on the malaria parasite have been confined to low dose attenuation of parasites in blood for transfusion purposes. There is no published information ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-018-9683-4
更新日期：2018-06-01 00:00:00
abstract：:Recent studies have reported that stem cells can be isolated from a wide range of tissues including bone marrow, fatty tissue, adipose tissue and placenta. Moreover, several studies also suggest that skin dermis could serve as a source of stem cells, but are of unclear phenotype. Therefore, we isolated and investigate...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9471-8
更新日期：2015-06-01 00:00:00
abstract：:Human sperm banking is an important procedure in the assisted reproductive technique centers. It entails sperm damage. The aim of this study was to investigate beneficial effect of Ceratonia siliqua (C. siliqua) supplement in freezing/thawing media on post thaw sperm parameters and sperm chromatin quality in normozoos...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-019-09779-2
更新日期：2019-09-01 00:00:00
abstract：:Human sperm cryopreservation is a common technique which is used in assisted reproductive technologies. Despite the existence of evidence supporting the production of ROS and DNA fragmentation during sperm cryopreservation, there is little and equivocal information about the cryopreservation effects on methylation of ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09828-1
更新日期：2020-09-01 00:00:00
abstract：:To compare the quality of the repair tissue in three-dimensional co-culture of human chondrocytes implanted in an in vivo model. Six cadaveric and five live human donors were included. Osteochondral biopsies from the donor knees were harvested for chondrocyte isolation. Fifty percent of cadaveric chondrocytes were exp...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9635-4
更新日期：2017-09-01 00:00:00
abstract：:During bone allograft processing, despite stringent donor screening and use of aseptic techniques, microbial invasion may occur due to the porous nature of the graft and cause potentially fatal infections. The aim of the present study was to prepare bone allograft with and without gentamicin and to compare bioburden a...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-019-09763-w
更新日期：2019-06-01 00:00:00
abstract：:Stem cell therapy is in constant need of new cell sources to conceive regenerative medicine approaches for diseases that are still without therapy. Scientists drew the attention toward amniotic membrane and amniotic fluid stem cells, since these sources possess many advantages: first of all as cells can be extracted f...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-014-9428-y
更新日期：2014-06-01 00:00:00
abstract：:Cell manufacturing for clinical applications is a unique form of biologics manufacturing that relies on maintenance of stringent work practices designed to ensure product consistency and prevent contamination by microorganisms or by another patient's cells. More extensive, prolonged laboratory processes involve greate...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-011-9276-y
更新日期：2012-12-01 00:00:00
abstract：:We evaluated the value of the 'alternative slices mirror image method' used in prostate tissue banking in terms of predicting the sampling of cancerous tissue while preserving the pathological prognostic information. The concordance of diagnosis between banked sections and their mirror image paraffin- sections was stu...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9284-y
更新日期：2012-12-01 00:00:00
abstract：:The aim of this study is to evaluate the beneficial effect of Myoinositol (MYO) supplement in freezing media on the post thaw sperm quality. Semen samples from 40 normozoospermic men were divided into two aliquots and frozen with simple or 2 mg/mL MYO supplemented freezing medium. Post thaw process including, computer...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-018-9731-0
更新日期：2019-03-01 00:00:00
abstract：:Allogeneic mandibular bone processed by the deep frozen method was used as a biologic crib for mandibular reconstruction. The allogeneic mandible is biocompatible, bioresorbable, of low antigenicity and provides the morphology for symphysis contour, angle of mandible and dental arch form. The particulate cancellous bo...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1023602717328
更新日期：2002-01-01 00:00:00
abstract：:The amnionic membrane is a rich source of multipotent mesenchymal stromal cells (hAMSC), which are readily available and show a potential use in regenerative medicine and tissue engineering. Before these cells can be applied clinically, careful characterization is necessary, especially as primary cells are known to ch...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-013-9415-8
更新日期：2014-06-01 00:00:00
abstract：:This is a report of a workshop held on the establishment of human research tissue banking which was held in Levi, Finland 21-24 March 2002. There were 21 participants from 7 European countries. This meeting was attended by representatives from academia, research tissue banks and from the Biotech and Pharmaceutical Ind...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1022811232250
更新日期：2002-01-01 00:00:00
abstract：:The Central Tissue Bank in Warsaw was established in 1963 and since then ionising radiation has been routinely applied to sterilise tissue grafts. Connective tissue grafts such as bone, cartilage, tendons, sclera, pericardium, skin, acellular dermis and amnion irradiated with a dose of 35 kGy in a (60)Co source and/or...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-005-0338-x
更新日期：2005-01-01 00:00:00
abstract：:A limited number of grafts produced in one batch is the main constrain to validate radiation sterilization dose of amnion and bone grafts according to ISO standard. The validation experiments done were according to ISO 13409 with a slight modification in sampling method. The experiments were carried out three times by...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1010129720363
更新日期：2000-01-01 00:00:00
abstract：:Brain donations are imperative for research; understanding possible barriers to entry is required to improve brain donation rates. While a few surveys have studied attitudes towards brain banking in patients with neurodegenerative disorders, none have surveyed patients with chronic neurological disorders but without n...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09819-2
更新日期：2020-06-01 00:00:00
abstract：:Delivering the promise of personalised medicine is the challenge that the current generation of scientists face. The variations in human physiology and disease are considerable, and designing appropriate strategies to deliver what has been promised will require access to tissue from a large number of volunteers. The N...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-010-9212-6
更新日期：2011-02-01 00:00:00
abstract：:The use of glycosaminoglycans (GAGs) as the second macromolecular component with collagen for the membrane preparation is based on the idea to create a biodegradable scaffold for new tissue. A hybrid collagen/hyaluronan membrane with specific bubble macrostructure was designed to serve as a synthetic dermal substitute...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/a:1010181925236
更新日期：2000-01-01 00:00:00
abstract：:Nowadays, there are four types of meniscal allografts known: fresh, cryopreserved, deep-frozen and lyophilized ones but only two of them are widely used in clinical practice. Use of different types of meniscal allografts still remains controversial due to preparation method, their biomechanical properties as well as c...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-013-9396-7
更新日期：2014-09-01 00:00:00
abstract：:Decellularised tissue allografts have been used in reconstructive surgical applications and transplantation for many years. Some of the current methods of sterilisation have a detrimental effect on the tissue graft structure and function. The anti-microbial activity of cupric ions and hydrogen peroxide (H2O2) are well...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9660-3
更新日期：2017-12-01 00:00:00
abstract：:To investigate the possible risk factors for failure of transplanted eye bank-prepared Descemet stripping automated endothelial keratoplasty (DSAEK) tissues. In a retrospective study between March 2011 and March 2019, all the failed DSAEK cases (131 cases) reported to the Central Eye Bank of Iran were compared with a ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09808-5
更新日期：2020-03-01 00:00:00
abstract：:The present experiment was conducted to evaluate the effect of kinetin on ram semen quality during cold storage. Ejaculates were collected using an artificial vagina from five Qezel rams. Ejaculates which met the criteria (volume of 0.75-2 ml; minimum spermatozoa concentration of 2.5 × 109 spermatozoa/ml and forward p...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-016-9604-3
更新日期：2018-03-01 00:00:00
abstract：:Although therapeutics derived from biological sources have been subjected to regulatory oversight for some time, the products used in transplantation procedures have historically been exempt from this oversight. These products have been viewed as being part of medical practice rather than as the result of mainstream p...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-006-9012-1
更新日期：2006-01-01 00:00:00
abstract：:Tissue banking is started in Thailand in 1979 at the Department of Orthopaedic Surgery, Siriraj Hospital, Bangkok. At that time tissues produced were freeze-dried bone allografts which were sterilized by ethylene oxide. In 1984, the freeze-dried tissue allograft project received an award from the National Research Cou...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1026542730541
更新日期：2000-01-01 00:00:00
abstract：:The aim of the present study was to compare the effects of two freezing methods, vapor phase and very rapid freezing, with and without cryoprotectant on semen parameters in men with normal semen criteria. Cryopreservation was done on semen samples from 31 men by two methods of vapor phase freezing and very rapid freez...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-012-9351-z
更新日期：2013-12-01 00:00:00
abstract：:Terminal sterilization of musculoskeletal allografts by gamma radiation minimizes the risk of disease transmission but impairs allograft mechanical properties. Commonly employed crosslinking agents can sterilize tissues without affecting mechanical properties adversely; however, these agents are toxic. Genipin is repo...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-012-9335-z
更新日期：2013-09-01 00:00:00
abstract：:Human epidermal melanocytes can be induced to form melanocyte spheroids and revert to immature characteristics by long-term trypsinization (LTT). To further explore the biological characteristics of melanocytes after LTT and to study the underlying mechanism. Melanocytes were subjected to long-term (2 h) trypsinizatio...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09866-9
更新日期：2020-09-25 00:00:00
