Abstract：
:The aim of this study is to evaluate the beneficial effect of Myoinositol (MYO) supplement in freezing media on the post thaw sperm quality. Semen samples from 40 normozoospermic men were divided into two aliquots and frozen with simple or 2 mg/mL MYO supplemented freezing medium. Post thaw process including, computer-assissted sperm analysis was used to analyze sperm motility and morphology. Reactive oxygen species was evaluated by the fluorometry of DCFH-DA, as well as total antioxidant capacity and lipid peroxidation were measured based on colorimetric assay by ELISA reader. Eventually, DNA fragmentation was assessed using TUNEL staining. MYO significantly improved progressive motility and normal morphology in treated samples (p < 0.05). Lipid peroxidation (malondialdehyde level) can be diminished in samples were frozen by MYO supplemented freezing media (p < 0.05). While MYO did not affect the amount of ROS (p > 0.05), it was associated with high values of total antioxidant capacity (p < 0.05). DNA integrity was significantly affected by MYO, as in MYO treated samples, DNA fragmentation was decreased compared to control ones (p < 0.001). The findings support the use of 2 mg/mL myoinositol supplemented freezing media in sperm cryopreservation to increase sperm quality after freezing-thawing procedures.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Mohammadi F,Varanloo N,Heydari Nasrabadi M,Vatannejad A,Amjadi FS,Javedani Masroor M,Bajelan L,Mehdizadeh M,Aflatoonian R,Zandieh Zdoi
10.1007/s10561-018-9731-0subject
Has Abstractpub_date
2019-03-01 00:00:00pages
77-86issue
1eissn
1389-9333issn
1573-6814pii
10.1007/s10561-018-9731-0journal_volume
20pub_type
杂志文章abstract：:Cerebral ischemic injury as the main manifestation of stroke can occur in stroke patients (70-80%). Nowadays, the main therapeutic strategy used for ischemic brain injury treatment aims to achieve reperfusion, neuroprotection, and neurorecovery. Also, angiogenesis as a therapeutic approach maybe represents a promising...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-020-09885-6
更新日期：2020-11-24 00:00:00
abstract：:A limited number of grafts produced in one batch is the main constrain to validate radiation sterilization dose of amnion and bone grafts according to ISO standard. The validation experiments done were according to ISO 13409 with a slight modification in sampling method. The experiments were carried out three times by...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1010129720363
更新日期：2000-01-01 00:00:00
abstract：:Tissue engineering using human cells and tissue has one of the greatest scientific and economical potential in the coming years. There are public concerns during the ongoing discussion about future trends in life sciences and if ethic boundaries might be respected sufficiently in the course of striving for industrial ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1026392429112
更新日期：2003-01-01 00:00:00
abstract：:Quality control and standardized preservation methods are essential in the field of transplantation. The International Organization for Standardization (ISO) has established a common set of manufacturing, trade and communications standards that are applicable worldwide and that provide the basis of a quality plan for ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1011576332362
更新日期：2001-01-01 00:00:00
abstract：:Human epidermal melanocytes can be induced to form melanocyte spheroids and revert to immature characteristics by long-term trypsinization (LTT). To further explore the biological characteristics of melanocytes after LTT and to study the underlying mechanism. Melanocytes were subjected to long-term (2 h) trypsinizatio...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09866-9
更新日期：2020-09-25 00:00:00
abstract：:The purpose of this study is to evaluate the reliability of cartilage digestion and fluorescein diacetate-ethidium bromide (FDA-EB) fluorescence staining for the detection of chondrocyte viability in osteochondral grafts. Sixteen fresh osteochondral grafts were harvested from pig knee condyles, and the articular carti...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-018-9685-2
更新日期：2018-09-01 00:00:00
abstract：:This study aimed to optimise methods of cryopreserving human articular cartilage (AC) tissue for the isolation of late chondrocytes. Human AC specimens from osteoarthritis patients who had undergone total knee replacement were used to optimise the chondrocyte isolation process and the choice of cryoprotective agent (C...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-012-9328-y
更新日期：2013-06-01 00:00:00
abstract：:In breast cancer, various clinical parameters are assessed to define clinical stage and thus obtain a more accurate prognosis. However, banks of tumor tissues are an important source of material for studies of risk of recurrence and of features governing clinical outcome in breast cancer. Although the heterogeneous ch...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-009-9124-5
更新日期：2009-11-01 00:00:00
abstract：:Human skin allografts are one of the best temporary biological coverings for severely burned patients. Cryopreserved (CPA) and glycerol-preserved (GPA) allografts are the most widely used types. This study compared the allograft efficiency of both preservation methods under the same conditions. To simulate actual clin...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9521-x
更新日期：2016-03-01 00:00:00
abstract：:Human amniotic membrane (hAM) represents a tissue that is well established as biomaterial in the clinics with potential for new applications in regenerative medicine. For tissue engineering (TE) strategies, cells are usually combined with inductive factors and a carrier substrate. We have previously recognized that hA...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9454-9
更新日期：2014-06-01 00:00:00
abstract：:In Malaysia, tissue banking activities began in Universiti Sains Malaysia (USM) Tissue Bank in early 1990s. Since then a few other bone banks have been set up in other government hospitals and institutions. However, these banks are not governed by the national authority. In addition there is no requirement set by the ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-010-9188-2
更新日期：2010-11-01 00:00:00
abstract：:Injured nerves cannot regenerate on their own, and a lack of engraftable human nerves has been a major obstacle in cell-based therapies for regenerating damaged nerves. A monolayer culture approach to obtain adherent neural stem cells from human embryonic stem cells (hESC-NSCs) was established, and the greatest number...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09816-5
更新日期：2020-06-01 00:00:00
abstract：:Insight into the pathogenesis of neurodegenerative disorders requires accurately categorized postmortem human brain tissue. This article introduces electronic tissue tracking and management as implemented at New York Brain Bank (NYBB) through processing of the brain at fresh state and storing standardized frozen sampl...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-008-9078-z
更新日期：2008-09-01 00:00:00
abstract：:Decidual stromal cells (DSCs) from maternal term placenta represent a potential source of cells for the treatment of cardiovascular and graft-versus-host diseases. However, it is not clear whether DSCs could be induced towards cardiomyocyte-like differentiation. We chose the placentas which should bred male new-baby. ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9493-x
更新日期：2015-09-01 00:00:00
abstract：:Appropriate animal models are critical to conduct translational studies of human disorders without variables that can confound clinical studies. Such analytic methods as patch-clamp electrophysiological and voltammetric recordings of neurons in brain slices require living brain tissue. In order to obtain viable tissue...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-013-9380-2
更新日期：2014-03-01 00:00:00
abstract：:Decellularised tissue allografts have been used in reconstructive surgical applications and transplantation for many years. Some of the current methods of sterilisation have a detrimental effect on the tissue graft structure and function. The anti-microbial activity of cupric ions and hydrogen peroxide (H2O2) are well...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9660-3
更新日期：2017-12-01 00:00:00
abstract：:The viscoelastic and inertial properties of the arterial wall are responsible for the arterial functional role in the cardiovascular system. Cryopreservation is widely used to preserve blood vessels for vascular reconstruction but it is controversially suspected to affect the dynamic behaviour of these allografts. The...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-005-0655-0
更新日期：2006-01-01 00:00:00
abstract：:To vitrify human amniotic membrane specimens so that the maximum of epithelial cells survives in order to develop a procedure for the eventual vitrification of posterior corneal lamellae without using cryoprotective agents. To assess different methods of tissue sample preparation preceding vitrification. In group 1, t...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-013-9388-7
更新日期：2014-03-01 00:00:00
abstract：:This study was conducted to compare the quantitative and qualitative indices of the donated corneas maintained in either Optisol-GS or Eusol-C storage media. In an ante-grade single blind study, two corneas from each donor with a death to preservation time of less than 30 h and with a minimum of "an apparent good corn...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9466-5
更新日期：2015-06-01 00:00:00
abstract：:A thick gingival biotype is a requisite for good periodontal health. It has important role in resisting trauma and subsequent gingival recession. The gingival thickness is a significant predictor of clinical outcome of periodontal surgeries. Various surgical procedures are used to increase the gingival thickness. The ...
journal_title：Cell and tissue banking
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10561-015-9524-7
更新日期：2016-03-01 00:00:00
abstract：BACKGROUND:Despite improved surgical techniques and the use of new medication, healing of corneal and conjunctival defects cannot always be achieved. In this connection the clinical use of human amnion, produced by different techniques, has represented a successful alternative for the past ten years. The purpose of the...
journal_title：Cell and tissue banking
pub_type： 临床试验,杂志文章
doi：10.1023/b:catb.0000022222.41304.de
更新日期：2004-01-01 00:00:00
abstract：:Recent studies have reported that stem cells can be isolated from a wide range of tissues including bone marrow, fatty tissue, adipose tissue and placenta. Moreover, several studies also suggest that skin dermis could serve as a source of stem cells, but are of unclear phenotype. Therefore, we isolated and investigate...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9471-8
更新日期：2015-06-01 00:00:00
abstract：:The Israel National Skin Bank (INSB) was founded jointly by the Israel Defense Forces (IDF) Medical Corps and the Ministry of Health in 1986. The prime purpose of the Skin Bank is to treat burn victims incurred at war or during mass casualty incidences. The INSB Protocol is comprised of international skin bank protoco...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1010183719662
更新日期：2000-01-01 00:00:00
abstract：:Although allografts for anterior cruciate ligament (ACL) replacement have shown advantages compared to autografts, their use is limited due to the risk of disease transmission and the limitations of available sterilization methods. Gamma sterilization has shown detrimental effects on graft properties at the high doses...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9289-6
更新日期：2012-08-01 00:00:00
abstract：:Recovered human and animal tissues are used extensively in surgery for wound repair and reconstruction. In preparation for the validation of chemical disinfection and radiation sterilization processes, studies were performed on the development and validation of quantitative bioburden recovery methods for human bone an...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-010-9238-9
更新日期：2012-03-01 00:00:00
abstract：:Delivering the promise of personalised medicine is the challenge that the current generation of scientists face. The variations in human physiology and disease are considerable, and designing appropriate strategies to deliver what has been promised will require access to tissue from a large number of volunteers. The N...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-010-9212-6
更新日期：2011-02-01 00:00:00
abstract：:The amnionic membrane is a rich source of multipotent mesenchymal stromal cells (hAMSC), which are readily available and show a potential use in regenerative medicine and tissue engineering. Before these cells can be applied clinically, careful characterization is necessary, especially as primary cells are known to ch...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-013-9415-8
更新日期：2014-06-01 00:00:00
abstract：:Perivascular cells are known to be ancestors of mesenchymal stem cells (MSCs) and can be obtained from heart, skin, bone marrow, eye, placenta and umbilical cord (UC). However detailed characterization of perivascular cells around the human UC vein and comparative analysis of them with MSCs haven't been done yet. In t...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9542-5
更新日期：2016-06-01 00:00:00
abstract：:In order to guarantee the required level of quality for our Bone &Tissue Banking, we evaluated a new CE marked container (CELLFLEX MacoPharma), for packing, transport, processing and storage of bones for therapeutic use. The use of CE marked containers is mandatory for organ and tissue containers (Medical Device Direc...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-004-1090-3
更新日期：2004-01-01 00:00:00
abstract：:The use of glycosaminoglycans (GAGs) as the second macromolecular component with collagen for the membrane preparation is based on the idea to create a biodegradable scaffold for new tissue. A hybrid collagen/hyaluronan membrane with specific bubble macrostructure was designed to serve as a synthetic dermal substitute...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/a:1010181925236
更新日期：2000-01-01 00:00:00
