Abstract:
:The Israel National Skin Bank (INSB) was founded jointly by the Israel Defense Forces (IDF) Medical Corps and the Ministry of Health in 1986. The prime purpose of the Skin Bank is to treat burn victims incurred at war or during mass casualty incidences. The INSB Protocol is comprised of international skin bank protocols and our previous and present research results. They provide the framework for selecting optimal guidelines for procurement, processing, preservation, storage and evaluation of transplantation performance of viable skin grafts. For evaluation and direct comparison of graft performance of glycerolized or cryopreserved skin stored for long periods, we have applied a mouse recipient model developed by us. This model assesses graft performance before the rejection process takes place. The in vivo design has inherent clinical relevance, which is especially appealing. Cryopreserved skin performed better than glycerolized skin (p > 0.027), but fresh skin performed significantly better than cryopreserved skin (p > 0.003), as analyzed by the Mann-Whitney non-parametric test. Then graft performance of skin specimens were cryopreserved by programmed or stepwise freezing and stored at -80 degrees C or in liquid nitrogen for 1 and 6-10 months was evaluated. The average score of skin preserved by programmed freezing and stored in liquid nitrogen is the highest for both storage periods. This method has a highly significant advantage (p < 0.007) over the others for 6-10 months storage, evaluated by graft adherence. Several interaction factors determine the quality of cryopreserved skin. Highly significant is the interaction factor/'combined effect' of sample variability with the method of cryopreservation or with the storage period. Finally, the results of paired comparison of selected histology criteria of cryopreserved to fresh skin indicated that storage of skin for up to 5 years did not impair significantly its performance compared to fresh skin, whereas, after six years of storage, there was a highly significant (p < 0.001) impairment in skin quality. We offer a simplified in vivo model and analysis for cryopreserved skin graft performance, suggesting that the evaluation procedures, which are issues of great interest in skin banking, may help future skin banks to make informed choices and decisions regarding quality issues.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Ben-Bassat H,Chaouat M,Zumai E,Segal N,Cinamon U,Ron M,Wexler MR,Eldad Adoi
10.1023/A:1010183719662keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
303-312issue
4eissn
1389-9333issn
1573-6814pii
313185journal_volume
1pub_type
杂志文章abstract::Human skin allografts are one of the best temporary biological coverings for severely burned patients. Cryopreserved (CPA) and glycerol-preserved (GPA) allografts are the most widely used types. This study compared the allograft efficiency of both preservation methods under the same conditions. To simulate actual clin...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-015-9521-x
更新日期:2016-03-01 00:00:00
abstract::Cerebral ischemic injury as the main manifestation of stroke can occur in stroke patients (70-80%). Nowadays, the main therapeutic strategy used for ischemic brain injury treatment aims to achieve reperfusion, neuroprotection, and neurorecovery. Also, angiogenesis as a therapeutic approach maybe represents a promising...
journal_title:Cell and tissue banking
pub_type: 杂志文章,评审
doi:10.1007/s10561-020-09885-6
更新日期:2020-11-24 00:00:00
abstract::A limited number of grafts produced in one batch is the main constrain to validate radiation sterilization dose of amnion and bone grafts according to ISO standard. The validation experiments done were according to ISO 13409 with a slight modification in sampling method. The experiments were carried out three times by...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/A:1010129720363
更新日期:2000-01-01 00:00:00
abstract::Tissue engineering using human cells and tissue has one of the greatest scientific and economical potential in the coming years. There are public concerns during the ongoing discussion about future trends in life sciences and if ethic boundaries might be respected sufficiently in the course of striving for industrial ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/A:1026392429112
更新日期:2003-01-01 00:00:00
abstract::The purpose of this study is to evaluate the reliability of cartilage digestion and fluorescein diacetate-ethidium bromide (FDA-EB) fluorescence staining for the detection of chondrocyte viability in osteochondral grafts. Sixteen fresh osteochondral grafts were harvested from pig knee condyles, and the articular carti...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-018-9685-2
更新日期:2018-09-01 00:00:00
abstract::Outcomes for patients with metastatic and recurrent osteosarcoma remain poor and a better understanding of the biology of this malignancy is critical to the development of prognostic biomarkers and novel therapies. The purpose of this study was to establish a biobank of osteosarcoma which has the potential of monitori...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-020-09831-6
更新日期:2020-09-01 00:00:00
abstract::Recently Bulgarian Bone Marrow Donors Registry (BBMDR) has been established and since August 2005 it has been a member of Bone Marrow Donors Worldwide. Currently the number of healthy donors included in the BBMDR is relatively low. All donors included in the BBMDR are typed for HLA-A, -B, -DRB loci. Phylogenetic analy...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-007-9046-z
更新日期:2008-12-01 00:00:00
abstract::Cellular stress to ejaculated spermatozoa such as cryopreservation is known to induce caspase-derived, apoptotic signaling. Therefore, the proenzymes and active forms of caspases 1, 3, 8 and 9 were examined by western blot technique in unfrozen and frozen human spermatozoa of infertility patients and of healthy donors...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-005-0276-7
更新日期:2006-01-01 00:00:00
abstract::Injured nerves cannot regenerate on their own, and a lack of engraftable human nerves has been a major obstacle in cell-based therapies for regenerating damaged nerves. A monolayer culture approach to obtain adherent neural stem cells from human embryonic stem cells (hESC-NSCs) was established, and the greatest number...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-020-09816-5
更新日期:2020-06-01 00:00:00
abstract::The present experiment was conducted to evaluate the effect of kinetin on ram semen quality during cold storage. Ejaculates were collected using an artificial vagina from five Qezel rams. Ejaculates which met the criteria (volume of 0.75-2 ml; minimum spermatozoa concentration of 2.5 × 109 spermatozoa/ml and forward p...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-016-9604-3
更新日期:2018-03-01 00:00:00
abstract::The regulatory framework of tissue banking introduces a number of requirements for monitoring cleanrooms for processing tissue or cell grafts. Although a number of requirements were clearly defined, some requirements are open for interpretation. This study aims to contribute to the interpretation of GMP or GTP guideli...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9355-8
更新日期:2013-12-01 00:00:00
abstract::There are controversial reports about cardiac differentiation potential of mesenchymal stem cells (MSCs), and there is still no well-defined protocol for the induction of cardiac differentiation. The effects of retinoic acid (RA) and dimethyl sulfoxide (DMSO) on the proliferation and differentiation of human fetal liv...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-015-9514-9
更新日期:2016-03-01 00:00:00
abstract::Cryopreserved (CryoPA) and Glycerol-preserved (GPA) skin allografts are commonly used in the treatment of severe burn injuries. However, comparable data on their differences in clinical outcome is scarce. This retrospective review aims to study the effect of allograft viability on clinical outcomes. The records of 48 ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-011-9254-4
更新日期:2012-06-01 00:00:00
abstract::The study aim was to identify risk factors for morphological rejection of aortic and pulmonary valves for transplantation that could be used to optimize donor selection. The files of all Dutch heart valve donors, donating in a 2.5 years period, whose hearts were processed at Heart Valve Bank Rotterdam, were reviewed f...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-011-9267-z
更新日期:2013-03-01 00:00:00
abstract::Repair of damaged cartilage usually requires replacement tissue or substitute material. Tissue engineering is a promising means to produce replacement cartilage from autologous or allogeneic cell sources. Scaffolds provide a three-dimensional (3D) structure that is essential for chondrocyte function and synthesis of c...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-005-5810-0
更新日期:2005-01-01 00:00:00
abstract::We describe a rapid and easy technique for the preparation of pre-stripped Descemet membrane (DM) endothelial keratoplasty (DMEK) tissue in the eye bank, without the use of dye or trephining by the eye bank technician. After drawing 2 radial marks 90° apart on the scleral rim of the cornea, a circumferential incision ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-019-09771-w
更新日期:2019-06-01 00:00:00
abstract::Human skin allografts can be preserved by different methods. In our clinical practice, human skin allografts are harvested on multi-organ and tissue donors, transferred at +4°C in Ringer Lactate, cryopreserved with 15% Glycerol and held in the vapor phase of a liquid nitrogen freezer until delivery to the burn center....
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-011-9239-3
更新日期:2012-03-01 00:00:00
abstract::Freeze-dried dura mater allograft (FDDMA) was evaluated as a cost-effective collagenous barrier using an experimental animal model to determine its biocompatibility and 'cell-occlusivity' potential. The FDDMA was processed at the Civil Hospital Ahmedabad, in accordance with the guidelines provided by the South-Eastern...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/B:CATB.0000007039.98845.bd
更新日期:2003-01-01 00:00:00
abstract::National Blood Service (NBS) Tissue Services (TS) operates living donor and deceased donor tissue banking programmes. The living bone donor programme operates in collaboration with 91 orthopaedic departments across the country and collects bone donations, in the form of surgically removed femoral heads (FHs), from ove...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-005-2362-2
更新日期:2006-01-01 00:00:00
abstract::Insight into the pathogenesis of neurodegenerative disorders requires accurately categorized postmortem human brain tissue. This article introduces electronic tissue tracking and management as implemented at New York Brain Bank (NYBB) through processing of the brain at fresh state and storing standardized frozen sampl...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-008-9078-z
更新日期:2008-09-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) are used for tissue regeneration in several pathological conditions, including autoimmune diseases. However, the optimal sources and culture requirements for these cells are still under investigation. Here, we compared mRNA expression in dermal MSCs (DMSCs) at passage (P) 3 and P5 to prov...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-017-9672-z
更新日期:2018-09-01 00:00:00
abstract::A thick gingival biotype is a requisite for good periodontal health. It has important role in resisting trauma and subsequent gingival recession. The gingival thickness is a significant predictor of clinical outcome of periodontal surgeries. Various surgical procedures are used to increase the gingival thickness. The ...
journal_title:Cell and tissue banking
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10561-015-9524-7
更新日期:2016-03-01 00:00:00
abstract::The aim of this study is to evaluate the beneficial effect of Myoinositol (MYO) supplement in freezing media on the post thaw sperm quality. Semen samples from 40 normozoospermic men were divided into two aliquots and frozen with simple or 2 mg/mL MYO supplemented freezing medium. Post thaw process including, computer...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-018-9731-0
更新日期:2019-03-01 00:00:00
abstract::During August and September of 2013, temperature data loggers were shipped to and from an AATB accredited and FDA registered allograft tissue processing facility in Belgrade, MT (Bacterin International, Inc.) to five warm climate cities (Dallas, TX, El Paso, TX, New Orleans, LA, Phoenix, AZ, and Tampa, FL). Shipping d...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-016-9578-1
更新日期:2016-12-01 00:00:00
abstract::Revision surgery of joint replacements is increasing and raises the demand for allograft bone since restoration of bone stock is crucial for longevity of implants. Proceedings of bone grafts influence the biological and mechanic properties differently. This study examines the effect of thermodisinfection on mechanic p...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-016-9567-4
更新日期:2016-09-01 00:00:00
abstract::Human epidermal melanocytes can be induced to form melanocyte spheroids and revert to immature characteristics by long-term trypsinization (LTT). To further explore the biological characteristics of melanocytes after LTT and to study the underlying mechanism. Melanocytes were subjected to long-term (2 h) trypsinizatio...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-020-09866-9
更新日期:2020-09-25 00:00:00
abstract::In India, the procurement of tissues for transplantation is governed by the Transplantation of Human Organs Act, 1994. However, although this law exists, it is primarily applied to organ transplantation and rules and regulations that are specific to tissue banking have yet to be developed.The Tata Memorial Hospital (T...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/B:CATB.0000007026.00604.97
更新日期:2003-01-01 00:00:00
abstract::Human amniotic membrane (hAM) represents a tissue that is well established as biomaterial in the clinics with potential for new applications in regenerative medicine. For tissue engineering (TE) strategies, cells are usually combined with inductive factors and a carrier substrate. We have previously recognized that hA...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-014-9454-9
更新日期:2014-06-01 00:00:00
abstract::Allogeneic mandibular bone processed by the deep frozen method was used as a biologic crib for mandibular reconstruction. The allogeneic mandible is biocompatible, bioresorbable, of low antigenicity and provides the morphology for symphysis contour, angle of mandible and dental arch form. The particulate cancellous bo...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/A:1023602717328
更新日期:2002-01-01 00:00:00
abstract::Corneal disease is the second cause of blindness in developing countries, where the number of corneal grafts needed by far exceeds the number available. In industrialized countries, although corneas are generally available for keratoplasty, onto inflamed and vascularized host beds they are often rejected despite immun...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-018-9742-x
更新日期:2019-03-01 00:00:00