Regeneration of sciatic nerves by transplanted microvesicles of human neural stem cells derived from embryonic stem cells.


:Injured nerves cannot regenerate on their own, and a lack of engraftable human nerves has been a major obstacle in cell-based therapies for regenerating damaged nerves. A monolayer culture approach to obtain adherent neural stem cells from human embryonic stem cells (hESC-NSCs) was established, and the greatest number of stemness characteristics were achieved by the eighth generation of hESC-NSCs (P8 hESC-NSCs). To overcome deficits in cell therapy, we used microvesicles secreted from P8 hESC-NSCs (hESC-NSC-MVs) instead of entire hESC-NSCs. To investigate the therapeutic efficacy of hESC-NSC-MVs in vitro, hESC-NSC-MVs were cocultured with dorsal root ganglia to determine the length of axons. In vivo, we transected the sciatic nerve in SD rats and created a 5-mm gap. A sciatic nerve defect was bridged using a silicone tube filled with hESC-NSC-MVs (45 μg) in the MVs group, P8 hESC-NSCs (1 × 106 single cells) in the cell group and PBS in the control group. The hESC-NSC-MVs group showed better morphological recovery and a significantly greater number of regenerated axons than the hESC-NSCs group 12 weeks after nerve injury. These results indicated that the hESC-NSC-MVs group had the greatest ability to repair and reconstruct nerve structure and function. As a result, hESC-NSC-MVs may have potential for applications in the field of nerve regenerative repair.


Cell Tissue Bank


Cell and tissue banking


Chen X,Ye K,Yu J,Gao J,Zhang L,Ji X,Chen T,Wang H,Dai Y,Tang B,Xu H,Sun X,Hu J




Has Abstract


2020-06-01 00:00:00














  • Evaluation and utility of new CE marked containers (CELLFLEX-MacoPharma) for bone bank.

    abstract::In order to guarantee the required level of quality for our Bone &Tissue Banking, we evaluated a new CE marked container (CELLFLEX MacoPharma), for packing, transport, processing and storage of bones for therapeutic use. The use of CE marked containers is mandatory for organ and tissue containers (Medical Device Direc...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Villalba R,Fornés G,Dueñas R,García A,Ariza A,Gómez-Villagrán JL

    更新日期:2004-01-01 00:00:00

  • ISBT 128: a global information standard.

    abstract::ISBT 128 is the global standard for the identification, labeling and information transfer of human blood, cell, tissue and organ products across international borders and disparate health care systems. The system has been designed and perfected over almost two decades to ensure accuracy, safety and efficiency for the ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Distler P

    更新日期:2010-11-01 00:00:00

  • The effect of cryopreservation on DNA methylation patterns of the chromosome 15q11-q13 region in human spermatozoa.

    abstract::Human sperm cryopreservation is a common technique which is used in assisted reproductive technologies. Despite the existence of evidence supporting the production of ROS and DNA fragmentation during sperm cryopreservation, there is little and equivocal information about the cryopreservation effects on methylation of ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Khosravizadeh Z,Hassanzadeh G,Tavakkoly Bazzaz J,Alizadeh F,Totonchi M,Salehi E,Khodamoradi K,Khanehzad M,Hosseini SR,Abolhassani F

    更新日期:2020-09-01 00:00:00

  • Qualification of serological infectious disease assays for the screening of samples from deceased tissue donors.

    abstract::Whilst some of the assays used for serological screening of post-mortem blood samples from deceased tissue donors in some countries have been specifically validated by the manufacturer for this purpose, a significant number of those currently in use globally have not. Although specificity has previously been considere...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Kitchen AD,Newham JA

    更新日期:2011-05-01 00:00:00

  • Setting up a Tissue Bank in India: The Tata Memorial Hospital Experience.

    abstract::In India, the procurement of tissues for transplantation is governed by the Transplantation of Human Organs Act, 1994. However, although this law exists, it is primarily applied to organ transplantation and rules and regulations that are specific to tissue banking have yet to be developed.The Tata Memorial Hospital (T...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Gajiwala AL

    更新日期:2003-01-01 00:00:00

  • A method of isolating viable chondrocytes with proliferative capacity from cryopreserved human articular cartilage.

    abstract::This study aimed to optimise methods of cryopreserving human articular cartilage (AC) tissue for the isolation of late chondrocytes. Human AC specimens from osteoarthritis patients who had undergone total knee replacement were used to optimise the chondrocyte isolation process and the choice of cryoprotective agent (C...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Xia Z,Duan X,Murray D,Triffitt JT,Price AJ

    更新日期:2013-06-01 00:00:00

  • The role of IL-6 in bone marrow (BM)-derived mesenchymal stem cells (MSCs) proliferation and chondrogenesis.

    abstract::Rheumatoid arthritis (RA) is the most common degenerative arthritic cartilage and represents a disease where the prospect of stem cell therapy offers considerable hope. Currently, bone marrow (BM) represents the major source of mesenchymal stem cells (MSCs) for cell therapy. In the pathology of RA, the pro-inflammator...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Wei H,Shen G,Deng X,Lou D,Sun B,Wu H,Long L,Ding T,Zhao J

    更新日期:2013-12-01 00:00:00

  • Establishing a cGMP pancreatic islet processing facility: the first experience in Iran.

    abstract::It has been predicted that one of the greatest increase in prevalence of diabetes will happen in the Middle East bear in the next decades. The aim of standard therapeutic strategies for diabetes is better control of complications. In contrast, some new strategies like cell and gene therapy have aimed to cure the disea...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Larijani B,Arjmand B,Amoli MM,Ao Z,Jafarian A,Mahdavi-Mazdah M,Ghanaati H,Baradar-Jalili R,Sharghi S,Norouzi-Javidan A,Aghayan HR

    更新日期:2012-12-01 00:00:00

  • Comparing quantitative and qualitative indices of the donated corneas maintained in Optisol-GS with those kept in Eusol-C.

    abstract::This study was conducted to compare the quantitative and qualitative indices of the donated corneas maintained in either Optisol-GS or Eusol-C storage media. In an ante-grade single blind study, two corneas from each donor with a death to preservation time of less than 30 h and with a minimum of "an apparent good corn...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Kanavi MR,Javadi MA,Chamani T,Fahim P,Javadi F

    更新日期:2015-06-01 00:00:00

  • Interaction of preservation methods and radiation sterilization in human skin processing, with particular insight on the impact of the final water content and collagen disruption. Part I: process validation, water activity and collagen changes in tissues

    abstract::Current regulatory requirements demand an in-depth understanding and validation of protocols used in tissue banking. The aim of this work was to characterize the quality of split thickness skin allografts cryopreserved or manufactured using highly concentrated solutions of glycerol (50, 85 or 98%), where tissue water ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Herson MR,Hamilton K,White J,Alexander D,Poniatowski S,O'Connor AJ,Werkmeister JA

    更新日期:2018-06-01 00:00:00

  • Establishment of a dynamic osteosarcoma biobank: Ruijin experience.

    abstract::Outcomes for patients with metastatic and recurrent osteosarcoma remain poor and a better understanding of the biology of this malignancy is critical to the development of prognostic biomarkers and novel therapies. The purpose of this study was to establish a biobank of osteosarcoma which has the potential of monitori...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Zang S,Wang J,Wen J,Bao Q,Shen Y,Zhang W

    更新日期:2020-09-01 00:00:00

  • Combination of retinoic acid, dimethyl sulfoxide and 5-azacytidine promotes cardiac differentiation of human fetal liver-derived mesenchymal stem cells.

    abstract::There are controversial reports about cardiac differentiation potential of mesenchymal stem cells (MSCs), and there is still no well-defined protocol for the induction of cardiac differentiation. The effects of retinoic acid (RA) and dimethyl sulfoxide (DMSO) on the proliferation and differentiation of human fetal liv...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Deng F,Lei H,Hu Y,He L,Fu H,Feng R,Feng P,Huang W,Wang X,Chang J

    更新日期:2016-03-01 00:00:00

  • Biocompatibility Studies of a New Biosynthetic Dermal Substitute Based on Collagen/Hyaluronan Conjugate.

    abstract::The use of glycosaminoglycans (GAGs) as the second macromolecular component with collagen for the membrane preparation is based on the idea to create a biodegradable scaffold for new tissue. A hybrid collagen/hyaluronan membrane with specific bubble macrostructure was designed to serve as a synthetic dermal substitute...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Koller J,Bakos D,Sadlonová I I

    更新日期:2000-01-01 00:00:00

  • Confirmation of cadaveric blood sample identity by DNA profiling using Short Tandem Repeat (STR) analysis.

    abstract::Blood samples collected from deceased tissue donors for mandatory transfusion microbiology testing may be taken either at the time of tissue donation, or residual samples may be retrieved from hospital laboratories where they were originally used for ante-mortem tests. In the latter case, sample labelling may not conf...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Warwick RM,Rushambuza FG,Brown J,Patel R,Tabb S,Poniatowski S,Ranson AJ,Brown CJ

    更新日期:2008-12-01 00:00:00

  • Defined serum- and xeno-free cryopreservation of mesenchymal stem cells.

    abstract::Mesenchymal stem cells (MSCs) have vast potential in cell therapy, and are experimentally used in the clinic. Therefore, it is critical to find a serum- and xeno-free cryopreservation method. The aim of this study was to compare two serum- and xeno-free cryoprotectants for MSCs. Adipose tissue MSCs (Ad-MSCs) and bone ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Al-Saqi SH,Saliem M,Quezada HC,Ekblad Å,Jonasson AF,Hovatta O,Götherström C

    更新日期:2015-06-01 00:00:00

  • Kinetin improves motility, viability and antioxidative parameters of ram semen during storage at refrigerator temperature.

    abstract::The present experiment was conducted to evaluate the effect of kinetin on ram semen quality during cold storage. Ejaculates were collected using an artificial vagina from five Qezel rams. Ejaculates which met the criteria (volume of 0.75-2 ml; minimum spermatozoa concentration of 2.5 × 109 spermatozoa/ml and forward p...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Zadeh Hashem E,Eslami M

    更新日期:2018-03-01 00:00:00

  • A new standardized immunofluorescence method for potency quantification (SMPQ) of human conjunctival cell cultures.

    abstract::The aim of this study is to set up a standardized and reproducible method to determine the potency (= stem cell content) of human conjunctival cell cultures by means of immunofluorescence-based analyses. This will help the development of new Advanced Therapy Medicinal Products (ATMPs) to use in future cell therapy cli...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Bertolin M,Breda C,Ferrari S,Lamon M,Ponzin D,Ferrari B,Barbaro V

    更新日期:2020-10-13 00:00:00

  • Chondrocyte suspension in fibrin glue.

    abstract::Autologous chondrocyte implantation has been shown to be a promising method for treatment of deep articular cartilage defects. The hyaline cartilage formed by implanted autologous chondrocytes has biomechanical properties similar to those of natural articular cartilage. Between June 2006 and September 2008 we performe...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Wysocka A,Mann K,Bursig H,Dec J,Gaździk TS

    更新日期:2010-05-01 00:00:00

  • Effect of low dose and moderate dose gamma irradiation on the mechanical properties of bone and soft tissue allografts.

    abstract::The increased use of allograft tissue for musculoskeletal repair has brought more focus to the safety of allogenic tissue and the efficacy of various sterilization techniques. Gamma irradiation is an effective method for providing terminal sterilization to biological tissue, but it is also reported to have deleterious...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Balsly CR,Cotter AT,Williams LA,Gaskins BD,Moore MA,Wolfinbarger L Jr

    更新日期:2008-12-01 00:00:00

  • Trephine- and dye-free technique for eye bank preparation of pre-stripped Descemet membrane endothelial keratoplasty tissue.

    abstract::We describe a rapid and easy technique for the preparation of pre-stripped Descemet membrane (DM) endothelial keratoplasty (DMEK) tissue in the eye bank, without the use of dye or trephining by the eye bank technician. After drawing 2 radial marks 90° apart on the scleral rim of the cornea, a circumferential incision ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Chamani T,Javadi MA,Kanavi MR

    更新日期:2019-06-01 00:00:00

  • A future vision of the International Atomic Energy Agency (IAEA) program on radiation and tissue banking for Asia and the Pacific and Latin American regions.

    abstract::In order to solve some of the problems that are affecting tissue banking activities in the world, a new program/project proposal could be prepared by the IAEA and interested Member States in order to implement it in 2009. The main objective of the new program/project proposal could be the following: To consolidate tis...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Morales Pedraza J

    更新日期:2009-05-01 00:00:00

  • Isolation, characterisation and comparative analysis of human umbilical cord vein perivascular cells and cord blood mesenchymal stem cells.

    abstract::Perivascular cells are known to be ancestors of mesenchymal stem cells (MSCs) and can be obtained from heart, skin, bone marrow, eye, placenta and umbilical cord (UC). However detailed characterization of perivascular cells around the human UC vein and comparative analysis of them with MSCs haven't been done yet. In t...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Gökçinar-Yagci B,Özyüncü Ö,Çelebi-Saltik B

    更新日期:2016-06-01 00:00:00

  • Stem cells from fetal membranes and amniotic fluid: markers for cell isolation and therapy.

    abstract::Stem cell therapy is in constant need of new cell sources to conceive regenerative medicine approaches for diseases that are still without therapy. Scientists drew the attention toward amniotic membrane and amniotic fluid stem cells, since these sources possess many advantages: first of all as cells can be extracted f...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章,评审


    authors: Pozzobon M,Piccoli M,De Coppi P

    更新日期:2014-06-01 00:00:00

  • Negative effect of combined cysteine and glutathione in soy lecithin-based extender on post-thawed ram spermatozoa.

    abstract::This study was conducted to evaluate the effect of combined cysteine and glutathione in soy lecithin-based semen extender on post-thawed ram sperm quality. A total of 28 ejaculates were collected twice a week (from four rams) during breeding season. In each replicate, semen samples (n = 4, one ejaculate for each ram) ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Zhandi M,Sharafi M

    更新日期:2015-09-01 00:00:00

  • Porcine bone grafts defatted by lipase: efficacy of defatting and assessment of cytocompatibility.

    abstract::Defatting is an important procedure for the preparation of bone grafts because lipids in bone grafts strongly influence the osteointegration. Lipases have been widely used in different fields. However, study on the application to defatting process for bone grafts preparation has never been found so far. In this study,...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Zhang N,Zhou M,Zhang Y,Wang X,Ma S,Dong L,Yang T,Ma L,Li B

    更新日期:2014-09-01 00:00:00

  • Enrichment of non-apoptotic human spermatozoa after cryopreservation by immunomagnetic cell sorting.

    abstract::Cryopreservation increases the rate of apoptotic spermatozoa with decreased capability to fertilise oocytes. In order to optimise the fertilisation rates, especially in assisted reproduction the use of apoptotic sperms should be avoided. Early events of apoptosis in cryopreserved spermatozoa are not detectable by conv...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Grunewald S,Paasch U,Glander HJ

    更新日期:2001-01-01 00:00:00

  • Validation of radiation sterilization dose for lyophilized amnion and bone grafts.

    abstract::A limited number of grafts produced in one batch is the main constrain to validate radiation sterilization dose of amnion and bone grafts according to ISO standard. The validation experiments done were according to ISO 13409 with a slight modification in sampling method. The experiments were carried out three times by...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Hilmy N,Febrida A,Basril A

    更新日期:2000-01-01 00:00:00

  • Viability of cryopreserved human skin allografts: effects of transport media and cryoprotectant.

    abstract::Human skin allografts can be preserved by different methods. In our clinical practice, human skin allografts are harvested on multi-organ and tissue donors, transferred at +4°C in Ringer Lactate, cryopreserved with 15% Glycerol and held in the vapor phase of a liquid nitrogen freezer until delivery to the burn center....

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Gaucher S,Elie C,Vérola O,Jarraya M

    更新日期:2012-03-01 00:00:00

  • The Israel National Skin Bank: Quality Assurance and Graft Performance of Stored Tissues.

    abstract::The Israel National Skin Bank (INSB) was founded jointly by the Israel Defense Forces (IDF) Medical Corps and the Ministry of Health in 1986. The prime purpose of the Skin Bank is to treat burn victims incurred at war or during mass casualty incidences. The INSB Protocol is comprised of international skin bank protoco...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Ben-Bassat H,Chaouat M,Zumai E,Segal N,Cinamon U,Ron M,Wexler MR,Eldad A

    更新日期:2000-01-01 00:00:00

  • Protective effects of mesenchymal stem cells on ischemic brain injury: therapeutic perspectives of regenerative medicine.

    abstract::Cerebral ischemic injury as the main manifestation of stroke can occur in stroke patients (70-80%). Nowadays, the main therapeutic strategy used for ischemic brain injury treatment aims to achieve reperfusion, neuroprotection, and neurorecovery. Also, angiogenesis as a therapeutic approach maybe represents a promising...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章,评审


    authors: Bagheri-Mohammadi S

    更新日期:2020-11-24 00:00:00