Regeneration of sciatic nerves by transplanted microvesicles of human neural stem cells derived from embryonic stem cells.


:Injured nerves cannot regenerate on their own, and a lack of engraftable human nerves has been a major obstacle in cell-based therapies for regenerating damaged nerves. A monolayer culture approach to obtain adherent neural stem cells from human embryonic stem cells (hESC-NSCs) was established, and the greatest number of stemness characteristics were achieved by the eighth generation of hESC-NSCs (P8 hESC-NSCs). To overcome deficits in cell therapy, we used microvesicles secreted from P8 hESC-NSCs (hESC-NSC-MVs) instead of entire hESC-NSCs. To investigate the therapeutic efficacy of hESC-NSC-MVs in vitro, hESC-NSC-MVs were cocultured with dorsal root ganglia to determine the length of axons. In vivo, we transected the sciatic nerve in SD rats and created a 5-mm gap. A sciatic nerve defect was bridged using a silicone tube filled with hESC-NSC-MVs (45 μg) in the MVs group, P8 hESC-NSCs (1 × 106 single cells) in the cell group and PBS in the control group. The hESC-NSC-MVs group showed better morphological recovery and a significantly greater number of regenerated axons than the hESC-NSCs group 12 weeks after nerve injury. These results indicated that the hESC-NSC-MVs group had the greatest ability to repair and reconstruct nerve structure and function. As a result, hESC-NSC-MVs may have potential for applications in the field of nerve regenerative repair.


Cell Tissue Bank


Cell and tissue banking


Chen X,Ye K,Yu J,Gao J,Zhang L,Ji X,Chen T,Wang H,Dai Y,Tang B,Xu H,Sun X,Hu J




Has Abstract


2020-06-01 00:00:00














  • Analysis of impact on tissue activity during COVID-19 outbreak: a survey of 8 banks in Spain.

    abstract::On March 19 World Health Organization declare the pandemic situation by outbreak coronavirus disease 2019 in the world. The pressure on the health care system has been very high in several countries. Spanish National Transplant Organization (ONT) have made many efforts in maintaining transplantation activity. Although...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章,多中心研究


    authors: Villalba R,Santos S,Martinez MJ,Díaz M,Pevida M,Cemborain A,Casares C,Mirabet V

    更新日期:2020-12-01 00:00:00

  • Chondrocyte suspension in fibrin glue.

    abstract::Autologous chondrocyte implantation has been shown to be a promising method for treatment of deep articular cartilage defects. The hyaline cartilage formed by implanted autologous chondrocytes has biomechanical properties similar to those of natural articular cartilage. Between June 2006 and September 2008 we performe...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Wysocka A,Mann K,Bursig H,Dec J,Gaździk TS

    更新日期:2010-05-01 00:00:00

  • The effect of cryopreservation on DNA methylation patterns of the chromosome 15q11-q13 region in human spermatozoa.

    abstract::Human sperm cryopreservation is a common technique which is used in assisted reproductive technologies. Despite the existence of evidence supporting the production of ROS and DNA fragmentation during sperm cryopreservation, there is little and equivocal information about the cryopreservation effects on methylation of ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Khosravizadeh Z,Hassanzadeh G,Tavakkoly Bazzaz J,Alizadeh F,Totonchi M,Salehi E,Khodamoradi K,Khanehzad M,Hosseini SR,Abolhassani F

    更新日期:2020-09-01 00:00:00

  • Risk factors associated with contamination of allograft valves in a tissue bank.

    abstract::The contamination of the transport solution used in cardiovascular allografts can occur from different sources. Risk factors associated with positive microbiological test of transport solution have not been reported previously. This study aimed to determine the risk factor for contamination of transport solution used ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Sawa B,Ribeiro VST,Kraft L,Wollmann LC,Pegoraro D,Suss PH,Tuon FF

    更新日期:2019-03-01 00:00:00

  • A new standardized immunofluorescence method for potency quantification (SMPQ) of human conjunctival cell cultures.

    abstract::The aim of this study is to set up a standardized and reproducible method to determine the potency (= stem cell content) of human conjunctival cell cultures by means of immunofluorescence-based analyses. This will help the development of new Advanced Therapy Medicinal Products (ATMPs) to use in future cell therapy cli...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Bertolin M,Breda C,Ferrari S,Lamon M,Ponzin D,Ferrari B,Barbaro V

    更新日期:2020-10-13 00:00:00

  • Co-culture of dedifferentiated and primary human chondrocytes obtained from cadaveric donor enhance the histological quality of repair tissue: an in-vivo animal study.

    abstract::To compare the quality of the repair tissue in three-dimensional co-culture of human chondrocytes implanted in an in vivo model. Six cadaveric and five live human donors were included. Osteochondral biopsies from the donor knees were harvested for chondrocyte isolation. Fifty percent of cadaveric chondrocytes were exp...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Olivos-Meza A,Velasquillo Martínez C,Olivos Díaz B,Landa-Solís C,Brittberg M,Pichardo Bahena R,Ortega Sanchez C,Martínez V,Alvarez Lara E,Ibarra-Ponce de León JC

    更新日期:2017-09-01 00:00:00

  • Maternal predictors and quality of umbilical cord blood units.

    abstract::The aim of the study was to determine the relationship between the maternal age at delivery and selected properties of the cord blood stem cells. The study included 50 pregnant women aged between 18 and 38 years in which spontaneous labors or elective cesarean sections were performed. Umbilical cord blood was collecte...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Bielec-Berek B,Jastrzębska-Stojko Ż,Drosdzol-Cop A,Jendyk C,Boruczkowski D,Ołdak T,Nowak-Brzezińska A,Stojko R

    更新日期:2018-03-01 00:00:00

  • Validation of radiation sterilization dose for lyophilized amnion and bone grafts.

    abstract::A limited number of grafts produced in one batch is the main constrain to validate radiation sterilization dose of amnion and bone grafts according to ISO standard. The validation experiments done were according to ISO 13409 with a slight modification in sampling method. The experiments were carried out three times by...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Hilmy N,Febrida A,Basril A

    更新日期:2000-01-01 00:00:00

  • Does supplementation of sperm freezing/thawing media with Ceratonia siliqua improve detrimental effect of cryopreservation on sperm parameters and chromatin quality in normozoospermic specimens?

    abstract::Human sperm banking is an important procedure in the assisted reproductive technique centers. It entails sperm damage. The aim of this study was to investigate beneficial effect of Ceratonia siliqua (C. siliqua) supplement in freezing/thawing media on post thaw sperm parameters and sperm chromatin quality in normozoos...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Faramarzi A,Aghaz F,Golestan Jahromi M,Bakhtiari M,Khazaei M

    更新日期:2019-09-01 00:00:00

  • The implementation of tissue banking experiences for setting up a cGMP cell manufacturing facility.

    abstract::Cell manufacturing for clinical applications is a unique form of biologics manufacturing that relies on maintenance of stringent work practices designed to ensure product consistency and prevent contamination by microorganisms or by another patient's cells. More extensive, prolonged laboratory processes involve greate...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章,评审


    authors: Arjmand B,Emami-Razavi SH,Larijani B,Norouzi-Javidan A,Aghayan HR

    更新日期:2012-12-01 00:00:00

  • Setting up a Tissue Bank in India: The Tata Memorial Hospital Experience.

    abstract::In India, the procurement of tissues for transplantation is governed by the Transplantation of Human Organs Act, 1994. However, although this law exists, it is primarily applied to organ transplantation and rules and regulations that are specific to tissue banking have yet to be developed.The Tata Memorial Hospital (T...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Gajiwala AL

    更新日期:2003-01-01 00:00:00

  • Effect of low dose and moderate dose gamma irradiation on the mechanical properties of bone and soft tissue allografts.

    abstract::The increased use of allograft tissue for musculoskeletal repair has brought more focus to the safety of allogenic tissue and the efficacy of various sterilization techniques. Gamma irradiation is an effective method for providing terminal sterilization to biological tissue, but it is also reported to have deleterious...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Balsly CR,Cotter AT,Williams LA,Gaskins BD,Moore MA,Wolfinbarger L Jr

    更新日期:2008-12-01 00:00:00

  • Sporicidal efficacy of genipin: a potential theoretical alternative for biomaterial and tissue graft sterilization.

    abstract::Terminal sterilization of musculoskeletal allografts by gamma radiation minimizes the risk of disease transmission but impairs allograft mechanical properties. Commonly employed crosslinking agents can sterilize tissues without affecting mechanical properties adversely; however, these agents are toxic. Genipin is repo...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Reich MS,Akkus O

    更新日期:2013-09-01 00:00:00

  • Supplementation of sperm freezing medium with myoinositol improve human sperm parameters and protects it against DNA fragmentation and apoptosis.

    abstract::The aim of this study is to evaluate the beneficial effect of Myoinositol (MYO) supplement in freezing media on the post thaw sperm quality. Semen samples from 40 normozoospermic men were divided into two aliquots and frozen with simple or 2 mg/mL MYO supplemented freezing medium. Post thaw process including, computer...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Mohammadi F,Varanloo N,Heydari Nasrabadi M,Vatannejad A,Amjadi FS,Javedani Masroor M,Bajelan L,Mehdizadeh M,Aflatoonian R,Zandieh Z

    更新日期:2019-03-01 00:00:00

  • When do tissues and cells become products? Regulatory oversight of emerging biological therapies.

    abstract::Although therapeutics derived from biological sources have been subjected to regulatory oversight for some time, the products used in transplantation procedures have historically been exempt from this oversight. These products have been viewed as being part of medical practice rather than as the result of mainstream p...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章,评审


    authors: Farrugia A

    更新日期:2006-01-01 00:00:00

  • The establishment of a network of European human research tissue banks.

    abstract::This is a report of a workshop held on the establishment of human research tissue banking which was held in Levi, Finland 21-24 March 2002. There were 21 participants from 7 European countries. This meeting was attended by representatives from academia, research tissue banks and from the Biotech and Pharmaceutical Ind...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Orr S,Alexandre E,Clark B,Combes R,Fels LM,Gray N,Jönsson-Rylander AC,Helin H,Koistinen J,Oinonen T,Richert L,Ravid R,Salonen J,Teesalu T,Thasler W,Trafford J,Van Der Valk J,Von Versen R,Weiss T,Womack C,Ylikomi T

    更新日期:2002-01-01 00:00:00

  • Enhancing gingival biotype through chorion membrane with innovative step in periodontal pocket therapy.

    abstract::A thick gingival biotype is a requisite for good periodontal health. It has important role in resisting trauma and subsequent gingival recession. The gingival thickness is a significant predictor of clinical outcome of periodontal surgeries. Various surgical procedures are used to increase the gingival thickness. The ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章,随机对照试验


    authors: Kothiwale S,Rathore A,Panjwani V

    更新日期:2016-03-01 00:00:00

  • Does prolonged post-mortem cold ischemic harvesting time influence cryopreserved pulmonary homograft tissue integrity?

    abstract::This study investigated cryopreserved pulmonary homograft (CPA) structural integrity after prolonged cold ischemic harvesting times in a juvenile sheep model. Three groups with different post-mortem cold ischemic harvesting times were studied, i.e. Group 1 (24 h, n = 10); group 2 (48 h, n = 10); group 3 (72 h, n = 10)...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Smit FE,Bester D,van den Heever JJ,Schlegel F,Botes L,Dohmen PM

    更新日期:2015-12-01 00:00:00

  • Comparing quantitative and qualitative indices of the donated corneas maintained in Optisol-GS with those kept in Eusol-C.

    abstract::This study was conducted to compare the quantitative and qualitative indices of the donated corneas maintained in either Optisol-GS or Eusol-C storage media. In an ante-grade single blind study, two corneas from each donor with a death to preservation time of less than 30 h and with a minimum of "an apparent good corn...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Kanavi MR,Javadi MA,Chamani T,Fahim P,Javadi F

    更新日期:2015-06-01 00:00:00

  • Donor exclusion in the National Blood Service Tissue Services living bone donor programme.

    abstract::National Blood Service (NBS) Tissue Services (TS) operates living donor and deceased donor tissue banking programmes. The living bone donor programme operates in collaboration with 91 orthopaedic departments across the country and collects bone donations, in the form of surgically removed femoral heads (FHs), from ove...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Pink F,Warwick RM,Purkis J,Pearson J

    更新日期:2006-01-01 00:00:00

  • ISBT 128: a global information standard.

    abstract::ISBT 128 is the global standard for the identification, labeling and information transfer of human blood, cell, tissue and organ products across international borders and disparate health care systems. The system has been designed and perfected over almost two decades to ensure accuracy, safety and efficiency for the ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Distler P

    更新日期:2010-11-01 00:00:00

  • Porcine bone grafts defatted by lipase: efficacy of defatting and assessment of cytocompatibility.

    abstract::Defatting is an important procedure for the preparation of bone grafts because lipids in bone grafts strongly influence the osteointegration. Lipases have been widely used in different fields. However, study on the application to defatting process for bone grafts preparation has never been found so far. In this study,...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Zhang N,Zhou M,Zhang Y,Wang X,Ma S,Dong L,Yang T,Ma L,Li B

    更新日期:2014-09-01 00:00:00

  • Evaluation and utility of new CE marked containers (CELLFLEX-MacoPharma) for bone bank.

    abstract::In order to guarantee the required level of quality for our Bone &Tissue Banking, we evaluated a new CE marked container (CELLFLEX MacoPharma), for packing, transport, processing and storage of bones for therapeutic use. The use of CE marked containers is mandatory for organ and tissue containers (Medical Device Direc...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Villalba R,Fornés G,Dueñas R,García A,Ariza A,Gómez-Villagrán JL

    更新日期:2004-01-01 00:00:00

  • Cleanrooms and tissue banking how happy I could be with either GMP or GTP?

    abstract::The regulatory framework of tissue banking introduces a number of requirements for monitoring cleanrooms for processing tissue or cell grafts. Although a number of requirements were clearly defined, some requirements are open for interpretation. This study aims to contribute to the interpretation of GMP or GTP guideli...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Klykens J,Pirnay JP,Verbeken G,Giet O,Baudoux E,Jashari R,Vanderkelen A,Ectors N

    更新日期:2013-12-01 00:00:00

  • Sterilization with electron beam irradiation influences the biomechanical properties and the early remodeling of tendon allografts for reconstruction of the anterior cruciate ligament (ACL).

    abstract::Although allografts for anterior cruciate ligament (ACL) replacement have shown advantages compared to autografts, their use is limited due to the risk of disease transmission and the limitations of available sterilization methods. Gamma sterilization has shown detrimental effects on graft properties at the high doses...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Schmidt T,Hoburg A,Broziat C,Smith MD,Gohs U,Pruss A,Scheffler S

    更新日期:2012-08-01 00:00:00

  • Application of sterilised human amnion for reconstruction of the ocular surface.

    abstract:BACKGROUND:Despite improved surgical techniques and the use of new medication, healing of corneal and conjunctival defects cannot always be achieved. In this connection the clinical use of human amnion, produced by different techniques, has represented a successful alternative for the past ten years. The purpose of the...

    journal_title:Cell and tissue banking

    pub_type: 临床试验,杂志文章


    authors: von Versen-Höynck F,Hesselbarth U,Möller DE

    更新日期:2004-01-01 00:00:00

  • Comparison of impact of two decontamination solutions on the viability of the cells in human amnion.

    abstract::Human amniotic membrane (HAM) is used as an allograft in regenerative medicine or as a source of pluripotent cells for stem cell research. Various decontamination protocols and solutions are used to sterilize HAM before its application, but little is known about the toxicity of disinfectants on HAM cells. In this stud...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Smeringaiova I,Trosan P,Mrstinova MB,Matecha J,Burkert J,Bednar J,Jirsova K

    更新日期:2017-09-01 00:00:00

  • Interaction of preservation methods and radiation sterilization in human skin processing, with particular insight on the impact of the final water content and collagen disruption. Part I: process validation, water activity and collagen changes in tissues

    abstract::Current regulatory requirements demand an in-depth understanding and validation of protocols used in tissue banking. The aim of this work was to characterize the quality of split thickness skin allografts cryopreserved or manufactured using highly concentrated solutions of glycerol (50, 85 or 98%), where tissue water ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Herson MR,Hamilton K,White J,Alexander D,Poniatowski S,O'Connor AJ,Werkmeister JA

    更新日期:2018-06-01 00:00:00

  • Preservation and sterilization methods of the meniscal allografts: literature review.

    abstract::Nowadays, there are four types of meniscal allografts known: fresh, cryopreserved, deep-frozen and lyophilized ones but only two of them are widely used in clinical practice. Use of different types of meniscal allografts still remains controversial due to preparation method, their biomechanical properties as well as c...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章,评审


    authors: Mickiewicz P,Binkowski M,Bursig H,Wróbel Z

    更新日期:2014-09-01 00:00:00

  • Assessment of bioburden on human and animal tissues: Part 1--results of method development and validation studies.

    abstract::Recovered human and animal tissues are used extensively in surgery for wound repair and reconstruction. In preparation for the validation of chemical disinfection and radiation sterilization processes, studies were performed on the development and validation of quantitative bioburden recovery methods for human bone an...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章


    authors: Kowalski JB,Mosley GA,Merritt K,Osborne J

    更新日期:2012-03-01 00:00:00