Abstract:
:Injured nerves cannot regenerate on their own, and a lack of engraftable human nerves has been a major obstacle in cell-based therapies for regenerating damaged nerves. A monolayer culture approach to obtain adherent neural stem cells from human embryonic stem cells (hESC-NSCs) was established, and the greatest number of stemness characteristics were achieved by the eighth generation of hESC-NSCs (P8 hESC-NSCs). To overcome deficits in cell therapy, we used microvesicles secreted from P8 hESC-NSCs (hESC-NSC-MVs) instead of entire hESC-NSCs. To investigate the therapeutic efficacy of hESC-NSC-MVs in vitro, hESC-NSC-MVs were cocultured with dorsal root ganglia to determine the length of axons. In vivo, we transected the sciatic nerve in SD rats and created a 5-mm gap. A sciatic nerve defect was bridged using a silicone tube filled with hESC-NSC-MVs (45 μg) in the MVs group, P8 hESC-NSCs (1 × 106 single cells) in the cell group and PBS in the control group. The hESC-NSC-MVs group showed better morphological recovery and a significantly greater number of regenerated axons than the hESC-NSCs group 12 weeks after nerve injury. These results indicated that the hESC-NSC-MVs group had the greatest ability to repair and reconstruct nerve structure and function. As a result, hESC-NSC-MVs may have potential for applications in the field of nerve regenerative repair.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Chen X,Ye K,Yu J,Gao J,Zhang L,Ji X,Chen T,Wang H,Dai Y,Tang B,Xu H,Sun X,Hu Jdoi
10.1007/s10561-020-09816-5subject
Has Abstractpub_date
2020-06-01 00:00:00pages
233-248issue
2eissn
1389-9333issn
1573-6814pii
10.1007/s10561-020-09816-5journal_volume
21pub_type
杂志文章abstract::To compare the quality of the repair tissue in three-dimensional co-culture of human chondrocytes implanted in an in vivo model. Six cadaveric and five live human donors were included. Osteochondral biopsies from the donor knees were harvested for chondrocyte isolation. Fifty percent of cadaveric chondrocytes were exp...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-017-9635-4
更新日期:2017-09-01 00:00:00
abstract::Revision surgery of joint replacements is increasing and raises the demand for allograft bone since restoration of bone stock is crucial for longevity of implants. Proceedings of bone grafts influence the biological and mechanic properties differently. This study examines the effect of thermodisinfection on mechanic p...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-016-9567-4
更新日期:2016-09-01 00:00:00
abstract::Musculo-skeletal allografts sterilized and deep frozen are among the most common human tissue to be preserved and utilized in modern medicine. The effects of a long deep freezing period on cortical bone has already been evaluated and found to be insignificant. However, there are no reports about the influences of a pr...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-008-9106-z
更新日期:2009-02-01 00:00:00
abstract::Malaria is one of the exclusion criteria used in selecting tissue donors and the absence of this information can lead to rejection of tissues for transplant. The studies on the malaria parasite have been confined to low dose attenuation of parasites in blood for transfusion purposes. There is no published information ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-018-9683-4
更新日期:2018-06-01 00:00:00
abstract::The regulatory framework of tissue banking introduces a number of requirements for monitoring cleanrooms for processing tissue or cell grafts. Although a number of requirements were clearly defined, some requirements are open for interpretation. This study aims to contribute to the interpretation of GMP or GTP guideli...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9355-8
更新日期:2013-12-01 00:00:00
abstract::Increasingly dental surgeons face the challenge of reconstruction of the height and/or thickness of the alveolar ridge as more and more patients wish to have permanent restoration of their dental defects based on intraosseous implants. Evaluation of human allogeneic bone tissue grafts in reconstruction of atrophied al...
journal_title:Cell and tissue banking
pub_type: 临床试验,杂志文章
doi:10.1007/s10561-015-9512-y
更新日期:2015-12-01 00:00:00
abstract::Tissue banking is started in Thailand in 1979 at the Department of Orthopaedic Surgery, Siriraj Hospital, Bangkok. At that time tissues produced were freeze-dried bone allografts which were sterilized by ethylene oxide. In 1984, the freeze-dried tissue allograft project received an award from the National Research Cou...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/A:1026542730541
更新日期:2000-01-01 00:00:00
abstract::We evaluated the value of the 'alternative slices mirror image method' used in prostate tissue banking in terms of predicting the sampling of cancerous tissue while preserving the pathological prognostic information. The concordance of diagnosis between banked sections and their mirror image paraffin- sections was stu...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-011-9284-y
更新日期:2012-12-01 00:00:00
abstract::Human skin allografts are one of the best temporary biological coverings for severely burned patients. Cryopreserved (CPA) and glycerol-preserved (GPA) allografts are the most widely used types. This study compared the allograft efficiency of both preservation methods under the same conditions. To simulate actual clin...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-015-9521-x
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:Despite improved surgical techniques and the use of new medication, healing of corneal and conjunctival defects cannot always be achieved. In this connection the clinical use of human amnion, produced by different techniques, has represented a successful alternative for the past ten years. The purpose of the...
journal_title:Cell and tissue banking
pub_type: 临床试验,杂志文章
doi:10.1023/b:catb.0000022222.41304.de
更新日期:2004-01-01 00:00:00
abstract::In Malaysia, tissue banking activities began in Universiti Sains Malaysia (USM) Tissue Bank in early 1990s. Since then a few other bone banks have been set up in other government hospitals and institutions. However, these banks are not governed by the national authority. In addition there is no requirement set by the ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-010-9188-2
更新日期:2010-11-01 00:00:00
abstract::The present experiment was conducted to evaluate the effect of kinetin on ram semen quality during cold storage. Ejaculates were collected using an artificial vagina from five Qezel rams. Ejaculates which met the criteria (volume of 0.75-2 ml; minimum spermatozoa concentration of 2.5 × 109 spermatozoa/ml and forward p...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-016-9604-3
更新日期:2018-03-01 00:00:00
abstract::Cryopreservation increases the rate of apoptotic spermatozoa with decreased capability to fertilise oocytes. In order to optimise the fertilisation rates, especially in assisted reproduction the use of apoptotic sperms should be avoided. Early events of apoptosis in cryopreserved spermatozoa are not detectable by conv...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/A:1020188913551
更新日期:2001-01-01 00:00:00
abstract::The purpose of this study is to evaluate the reliability of cartilage digestion and fluorescein diacetate-ethidium bromide (FDA-EB) fluorescence staining for the detection of chondrocyte viability in osteochondral grafts. Sixteen fresh osteochondral grafts were harvested from pig knee condyles, and the articular carti...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-018-9685-2
更新日期:2018-09-01 00:00:00
abstract::The viscoelastic and inertial properties of the arterial wall are responsible for the arterial functional role in the cardiovascular system. Cryopreservation is widely used to preserve blood vessels for vascular reconstruction but it is controversially suspected to affect the dynamic behaviour of these allografts. The...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-005-0655-0
更新日期:2006-01-01 00:00:00
abstract::Osteochondral defects can degenerate into osteoarthritis and currently there are no good treatment alternatives available to most Orthopaedic surgeons. Osteochondral allografting can restore damaged joint surfaces but its clinical use is limited by poor access to high quality tissue. Vitrification of osteochondral tis...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-006-9023-y
更新日期:2007-01-01 00:00:00
abstract::Recovered human and animal tissues are used extensively in surgery for wound repair and reconstruction. In preparation for the validation of chemical disinfection and radiation sterilization processes, studies were performed on the development and validation of quantitative bioburden recovery methods for human bone an...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-010-9238-9
更新日期:2012-03-01 00:00:00
abstract::Perivascular cells are known to be ancestors of mesenchymal stem cells (MSCs) and can be obtained from heart, skin, bone marrow, eye, placenta and umbilical cord (UC). However detailed characterization of perivascular cells around the human UC vein and comparative analysis of them with MSCs haven't been done yet. In t...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-015-9542-5
更新日期:2016-06-01 00:00:00
abstract::The increased use of allograft tissue for musculoskeletal repair has brought more focus to the safety of allogenic tissue and the efficacy of various sterilization techniques. Gamma irradiation is an effective method for providing terminal sterilization to biological tissue, but it is also reported to have deleterious...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-008-9069-0
更新日期:2008-12-01 00:00:00
abstract::Cellular stress to ejaculated spermatozoa such as cryopreservation is known to induce caspase-derived, apoptotic signaling. Therefore, the proenzymes and active forms of caspases 1, 3, 8 and 9 were examined by western blot technique in unfrozen and frozen human spermatozoa of infertility patients and of healthy donors...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-005-0276-7
更新日期:2006-01-01 00:00:00
abstract::A limited number of grafts produced in one batch is the main constrain to validate radiation sterilization dose of amnion and bone grafts according to ISO standard. The validation experiments done were according to ISO 13409 with a slight modification in sampling method. The experiments were carried out three times by...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/A:1010129720363
更新日期:2000-01-01 00:00:00
abstract::Human skin allografts can be preserved by different methods. In our clinical practice, human skin allografts are harvested on multi-organ and tissue donors, transferred at +4°C in Ringer Lactate, cryopreserved with 15% Glycerol and held in the vapor phase of a liquid nitrogen freezer until delivery to the burn center....
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-011-9239-3
更新日期:2012-03-01 00:00:00
abstract::The aim of the study was to determine the relationship between the maternal age at delivery and selected properties of the cord blood stem cells. The study included 50 pregnant women aged between 18 and 38 years in which spontaneous labors or elective cesarean sections were performed. Umbilical cord blood was collecte...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-017-9657-y
更新日期:2018-03-01 00:00:00
abstract::To analyze the data of the adverse events collected in a single major eye bank (EFS Bourgogne Franche Comté, Besançon, France) for the year 2013 and to report the French data of biovigilance provided by the French National Agency for Medicines and Health Products Safety (ANSM) between 2010 and 2013. we have set up a s...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-016-9593-2
更新日期:2017-03-01 00:00:00
abstract::Homograft or allograft tissue has been available for use as replacement for diseased valves or reconstruction of major vessels for decades. However, with respect to replacement of diseased valvular tissue the search for the ideal valve still continues. In this review we will discuss the clinical indications, surgical ...
journal_title:Cell and tissue banking
pub_type: 杂志文章,评审
doi:10.1007/s10561-006-9009-9
更新日期:2006-01-01 00:00:00
abstract::Tissue and organ transplantation is one of the most promising treatments for some incurable diseases. Nowadays, transplantation is the common therapy in many countries. Unfortunately, availability of donated tissues and organs is limited. There are several factors which may affect donation rate for instance; social fa...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-014-9473-6
更新日期:2015-06-01 00:00:00
abstract::This study was conducted to compare the quantitative and qualitative indices of the donated corneas maintained in either Optisol-GS or Eusol-C storage media. In an ante-grade single blind study, two corneas from each donor with a death to preservation time of less than 30 h and with a minimum of "an apparent good corn...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-014-9466-5
更新日期:2015-06-01 00:00:00
abstract::Residual chemicals that are presented during tissue processing in human tissue banks can be a risk for the allograft recipient. Determine the residual concentrations of the antibiotics and detergent used in the process of human decellularized tissue-engineered heart valves stored in isotonic saline solution up to 18 m...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-020-09856-x
更新日期:2020-12-01 00:00:00
abstract::On March 19 World Health Organization declare the pandemic situation by outbreak coronavirus disease 2019 in the world. The pressure on the health care system has been very high in several countries. Spanish National Transplant Organization (ONT) have made many efforts in maintaining transplantation activity. Although...
journal_title:Cell and tissue banking
pub_type: 杂志文章,多中心研究
doi:10.1007/s10561-020-09853-0
更新日期:2020-12-01 00:00:00
abstract::Rheumatoid arthritis (RA) is the most common degenerative arthritic cartilage and represents a disease where the prospect of stem cell therapy offers considerable hope. Currently, bone marrow (BM) represents the major source of mesenchymal stem cells (MSCs) for cell therapy. In the pathology of RA, the pro-inflammator...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9354-9
更新日期:2013-12-01 00:00:00