Reliability of cartilage digestion and FDA-EB fluorescence staining for the detection of chondrocyte viability in osteochondral grafts.

Abstract:

:The purpose of this study is to evaluate the reliability of cartilage digestion and fluorescein diacetate-ethidium bromide (FDA-EB) fluorescence staining for the detection of chondrocyte viability in osteochondral grafts. Sixteen fresh osteochondral grafts were harvested from pig knee condyles, and the articular cartilage tissue was preserved. Each cartilage graft was cut into two 70-µm thick pieces and randomly allocated to Group A or Group B. The cell viability of Group A was detected using FDA-EB fluorescence staining of the digested cartilage, and the viability of Group B was detected with FDA-EB fluorescence staining of cartilage sections. Comparisons of chondrocyte viability and correlation analyses of the two groups were performed using the paired sample t test and Pearson correlation test, respectively. No significant difference was found in the chondrocyte viability between Groups A and B (p > 0.05), and a strong correlation was observed (r = 0.70, p < 0.05). Therefore, cartilage digestion with FDA-EB fluorescence staining is a reliable method for detecting chondrocyte viability in osteochondral grafts.

journal_name

Cell Tissue Bank

journal_title

Cell and tissue banking

authors

Qi J,Cao F,Han Y,Xie D,Song H,Chen B,Zhou L

doi

10.1007/s10561-018-9685-2

subject

Has Abstract

pub_date

2018-09-01 00:00:00

pages

399-404

issue

3

eissn

1389-9333

issn

1573-6814

pii

10.1007/s10561-018-9685-2

journal_volume

19

pub_type

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