Abstract:
:Although allografts for anterior cruciate ligament (ACL) replacement have shown advantages compared to autografts, their use is limited due to the risk of disease transmission and the limitations of available sterilization methods. Gamma sterilization has shown detrimental effects on graft properties at the high doses required for sufficient pathogen inactivation. In our previous in vitro study on human patellar tendon allografts, Electron beam (Ebeam) irradiation showed less detrimental effects compared to gamma sterilization (Hoburg et al. in Am J Sports Med 38(6):1134-1140, 2010). To investigate the biological healing and restoration of the mechanical properties of a 34 kGy Ebeam treated tendon allograft twenty-four sheep underwent ACL replacement with either a 34 kGy Ebeam treated allograft or a non-sterilized fresh frozen allograft. Biomechanical testing of stiffness, ultimate failure load and AP-laxity as well as histological analysis to investigate cell, vessel and myofibroblast-density were performed after 6 and 12 weeks. Native sheep ACL and hamstring tendons (HAT, each n = 9) served as controls. The results of a previous study analyzing the remodeling of fresh frozen allografts (n = 12) and autografts (Auto, n = 18) with the same study design were also included in the analysis. Statistics were performed using Mann-Whitney U test followed by Bonferroni-Holm correction. Results showed significantly decreased biomechanical properties during the early remodeling period in Ebeam treated grafts and this was accompanied with an increased remodeling activity. There was no recovery of biomechanical function from 6 to 12 weeks in this group in contrast to the results observed in fresh frozen allografts and autografts. Therefore, high dose Ebeam irradiation investigated in this paper cannot be recommended for soft tissue allograft sterilization.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Schmidt T,Hoburg A,Broziat C,Smith MD,Gohs U,Pruss A,Scheffler Sdoi
10.1007/s10561-011-9289-6subject
Has Abstractpub_date
2012-08-01 00:00:00pages
387-400issue
3eissn
1389-9333issn
1573-6814journal_volume
13pub_type
杂志文章abstract::Outcomes for patients with metastatic and recurrent osteosarcoma remain poor and a better understanding of the biology of this malignancy is critical to the development of prognostic biomarkers and novel therapies. The purpose of this study was to establish a biobank of osteosarcoma which has the potential of monitori...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-020-09831-6
更新日期:2020-09-01 00:00:00
abstract::There have been various studies about the acellular nerve allograft (ANA) as the alternative of autologous nerve graft in the treatment of peripheral nerve defects. As well as the decellularization process methods of ANA, the various enhancement methods of regeneration of the grafted ANA were investigated. The chondro...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-019-09770-x
更新日期:2019-06-01 00:00:00
abstract::Homograft or allograft tissue has been available for use as replacement for diseased valves or reconstruction of major vessels for decades. However, with respect to replacement of diseased valvular tissue the search for the ideal valve still continues. In this review we will discuss the clinical indications, surgical ...
journal_title:Cell and tissue banking
pub_type: 杂志文章,评审
doi:10.1007/s10561-006-9009-9
更新日期:2006-01-01 00:00:00
abstract::Defatting is an important procedure for the preparation of bone grafts because lipids in bone grafts strongly influence the osteointegration. Lipases have been widely used in different fields. However, study on the application to defatting process for bone grafts preparation has never been found so far. In this study,...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-013-9391-z
更新日期:2014-09-01 00:00:00
abstract::Cryopreservation increases the rate of apoptotic spermatozoa with decreased capability to fertilise oocytes. In order to optimise the fertilisation rates, especially in assisted reproduction the use of apoptotic sperms should be avoided. Early events of apoptosis in cryopreserved spermatozoa are not detectable by conv...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/A:1020188913551
更新日期:2001-01-01 00:00:00
abstract::Decidual stromal cells (DSCs) from maternal term placenta represent a potential source of cells for the treatment of cardiovascular and graft-versus-host diseases. However, it is not clear whether DSCs could be induced towards cardiomyocyte-like differentiation. We chose the placentas which should bred male new-baby. ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-015-9493-x
更新日期:2015-09-01 00:00:00
abstract::Residual chemicals that are presented during tissue processing in human tissue banks can be a risk for the allograft recipient. Determine the residual concentrations of the antibiotics and detergent used in the process of human decellularized tissue-engineered heart valves stored in isotonic saline solution up to 18 m...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-020-09856-x
更新日期:2020-12-01 00:00:00
abstract::In this study, the efficiency of the "Needle Immersed Vitrification" technique was tested on cryopreserved feline ovarian tissue. For vitrification, ovarian fragments (0.5-1.5 mm2) from each ovary were collected; the grafts were exposed to 7.5-15% ethylene glycol and 7.5-15% dimethyl sulfoxide at room temperature and ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-017-9663-0
更新日期:2018-03-01 00:00:00
abstract::The viscoelastic and inertial properties of the arterial wall are responsible for the arterial functional role in the cardiovascular system. Cryopreservation is widely used to preserve blood vessels for vascular reconstruction but it is controversially suspected to affect the dynamic behaviour of these allografts. The...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-005-0655-0
更新日期:2006-01-01 00:00:00
abstract::The contamination of the transport solution used in cardiovascular allografts can occur from different sources. Risk factors associated with positive microbiological test of transport solution have not been reported previously. This study aimed to determine the risk factor for contamination of transport solution used ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-019-09754-x
更新日期:2019-03-01 00:00:00
abstract::Since there is no upper age limit for general organ donation, unlike heart valve donation, and since a quarter of all organ donors are 65 years and older, we examined whether the heart valves from these donors are suitable as allografts. In the period 1999-2004 the aortic valve and pulmonary valve of 100 organ donors ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-007-9052-1
更新日期:2008-03-01 00:00:00
abstract::During bone allograft processing, despite stringent donor screening and use of aseptic techniques, microbial invasion may occur due to the porous nature of the graft and cause potentially fatal infections. The aim of the present study was to prepare bone allograft with and without gentamicin and to compare bioburden a...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-019-09763-w
更新日期:2019-06-01 00:00:00
abstract::Blood samples collected from deceased tissue donors for mandatory transfusion microbiology testing may be taken either at the time of tissue donation, or residual samples may be retrieved from hospital laboratories where they were originally used for ante-mortem tests. In the latter case, sample labelling may not conf...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-008-9071-6
更新日期:2008-12-01 00:00:00
abstract::Rheumatoid arthritis (RA) is the most common degenerative arthritic cartilage and represents a disease where the prospect of stem cell therapy offers considerable hope. Currently, bone marrow (BM) represents the major source of mesenchymal stem cells (MSCs) for cell therapy. In the pathology of RA, the pro-inflammator...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9354-9
更新日期:2013-12-01 00:00:00
abstract::The regulatory framework of tissue banking introduces a number of requirements for monitoring cleanrooms for processing tissue or cell grafts. Although a number of requirements were clearly defined, some requirements are open for interpretation. This study aims to contribute to the interpretation of GMP or GTP guideli...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9355-8
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND:Despite improved surgical techniques and the use of new medication, healing of corneal and conjunctival defects cannot always be achieved. In this connection the clinical use of human amnion, produced by different techniques, has represented a successful alternative for the past ten years. The purpose of the...
journal_title:Cell and tissue banking
pub_type: 临床试验,杂志文章
doi:10.1023/b:catb.0000022222.41304.de
更新日期:2004-01-01 00:00:00
abstract::This study investigated cryopreserved pulmonary homograft (CPA) structural integrity after prolonged cold ischemic harvesting times in a juvenile sheep model. Three groups with different post-mortem cold ischemic harvesting times were studied, i.e. Group 1 (24 h, n = 10); group 2 (48 h, n = 10); group 3 (72 h, n = 10)...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-015-9500-2
更新日期:2015-12-01 00:00:00
abstract::We evaluated the value of the 'alternative slices mirror image method' used in prostate tissue banking in terms of predicting the sampling of cancerous tissue while preserving the pathological prognostic information. The concordance of diagnosis between banked sections and their mirror image paraffin- sections was stu...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-011-9284-y
更新日期:2012-12-01 00:00:00
abstract::To investigate the possible risk factors for failure of transplanted eye bank-prepared Descemet stripping automated endothelial keratoplasty (DSAEK) tissues. In a retrospective study between March 2011 and March 2019, all the failed DSAEK cases (131 cases) reported to the Central Eye Bank of Iran were compared with a ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-020-09808-5
更新日期:2020-03-01 00:00:00
abstract::In India, the procurement of tissues for transplantation is governed by the Transplantation of Human Organs Act, 1994. However, although this law exists, it is primarily applied to organ transplantation and rules and regulations that are specific to tissue banking have yet to be developed.The Tata Memorial Hospital (T...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/B:CATB.0000007026.00604.97
更新日期:2003-01-01 00:00:00
abstract::Increasingly dental surgeons face the challenge of reconstruction of the height and/or thickness of the alveolar ridge as more and more patients wish to have permanent restoration of their dental defects based on intraosseous implants. Evaluation of human allogeneic bone tissue grafts in reconstruction of atrophied al...
journal_title:Cell and tissue banking
pub_type: 临床试验,杂志文章
doi:10.1007/s10561-015-9512-y
更新日期:2015-12-01 00:00:00
abstract::This is a report of a workshop held on the establishment of human research tissue banking which was held in Levi, Finland 21-24 March 2002. There were 21 participants from 7 European countries. This meeting was attended by representatives from academia, research tissue banks and from the Biotech and Pharmaceutical Ind...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/A:1022811232250
更新日期:2002-01-01 00:00:00
abstract::Human epidermal melanocytes can be induced to form melanocyte spheroids and revert to immature characteristics by long-term trypsinization (LTT). To further explore the biological characteristics of melanocytes after LTT and to study the underlying mechanism. Melanocytes were subjected to long-term (2 h) trypsinizatio...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-020-09866-9
更新日期:2020-09-25 00:00:00
abstract::Osteochondral defects can degenerate into osteoarthritis and currently there are no good treatment alternatives available to most Orthopaedic surgeons. Osteochondral allografting can restore damaged joint surfaces but its clinical use is limited by poor access to high quality tissue. Vitrification of osteochondral tis...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-006-9023-y
更新日期:2007-01-01 00:00:00
abstract::To analyze the data of the adverse events collected in a single major eye bank (EFS Bourgogne Franche Comté, Besançon, France) for the year 2013 and to report the French data of biovigilance provided by the French National Agency for Medicines and Health Products Safety (ANSM) between 2010 and 2013. we have set up a s...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-016-9593-2
更新日期:2017-03-01 00:00:00
abstract::Cellular stress to ejaculated spermatozoa such as cryopreservation is known to induce caspase-derived, apoptotic signaling. Therefore, the proenzymes and active forms of caspases 1, 3, 8 and 9 were examined by western blot technique in unfrozen and frozen human spermatozoa of infertility patients and of healthy donors...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-005-0276-7
更新日期:2006-01-01 00:00:00
abstract::Appropriate animal models are critical to conduct translational studies of human disorders without variables that can confound clinical studies. Such analytic methods as patch-clamp electrophysiological and voltammetric recordings of neurons in brain slices require living brain tissue. In order to obtain viable tissue...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-013-9380-2
更新日期:2014-03-01 00:00:00
abstract::Tissue engineering using human cells and tissue has one of the greatest scientific and economical potential in the coming years. There are public concerns during the ongoing discussion about future trends in life sciences and if ethic boundaries might be respected sufficiently in the course of striving for industrial ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/A:1026392429112
更新日期:2003-01-01 00:00:00
abstract::Malaria is one of the exclusion criteria used in selecting tissue donors and the absence of this information can lead to rejection of tissues for transplant. The studies on the malaria parasite have been confined to low dose attenuation of parasites in blood for transfusion purposes. There is no published information ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-018-9683-4
更新日期:2018-06-01 00:00:00
abstract::Whilst some of the assays used for serological screening of post-mortem blood samples from deceased tissue donors in some countries have been specifically validated by the manufacturer for this purpose, a significant number of those currently in use globally have not. Although specificity has previously been considere...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-010-9175-7
更新日期:2011-05-01 00:00:00