Confirmation of cadaveric blood sample identity by DNA profiling using Short Tandem Repeat (STR) analysis.

Abstract:

:Blood samples collected from deceased tissue donors for mandatory transfusion microbiology testing may be taken either at the time of tissue donation, or residual samples may be retrieved from hospital laboratories where they were originally used for ante-mortem tests. In the latter case, sample labelling may not conform to the required standard, which stipulates that three independent identifiers be provided. If no alternative adequately labelled sample is available for testing the donated tissues may have to be discarded, which can adversely affect tissue sufficiency. An alternative method to ensure that the blood sample to be tested is from the intended deceased donor is to confirm the identity of the blood sample by Deoxyribonucleic Nucleic Acid (DNA) Short Tandem Repeats (STR) analysis, then comparing the DNA profile with the DNA from the donated tissues. If the two DNA profiles are identical, probability calculations can demonstrate the chance of the two samples of DNA being from the same or different individuals. The authors have used this approach to salvage deceased tissue donations.

journal_name

Cell Tissue Bank

journal_title

Cell and tissue banking

authors

Warwick RM,Rushambuza FG,Brown J,Patel R,Tabb S,Poniatowski S,Ranson AJ,Brown CJ

doi

10.1007/s10561-008-9071-6

subject

Has Abstract

pub_date

2008-12-01 00:00:00

pages

323-8

issue

4

eissn

1389-9333

issn

1573-6814

journal_volume

9

pub_type

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