Abstract：
:Decidual stromal cells (DSCs) from maternal term placenta represent a potential source of cells for the treatment of cardiovascular and graft-versus-host diseases. However, it is not clear whether DSCs could be induced towards cardiomyocyte-like differentiation. We chose the placentas which should bred male new-baby. We isolated DSCs from placenta by tissue adherence. The morphology, immunophenotype, and multi-lineage potential were analyzed. Karyotype analysis (G-band) was performed to determine the source and karyotype stability of DSCs. DSCs were induced by 5-azacytidine. Expression of Myf5, α-cardiac actin, Cardiac troponin T (cTnT) and GAPDH was assessed by PCR, and cTnT expression was also analyzed by immunofluorescence. Karyotype analyses indicated that cells were derived from the maternal matrix. After induction with 5-azacytidine, DSCs expressed the cardiac-specific markers Myf5, myogenin and cTnT, indicating differentiation towards cardiomyocyte-like cells.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Chen G,Yue A,Ruan Z,Yin Y,Wang R,Ren Y,Zhu Ldoi
10.1007/s10561-015-9493-xsubject
Has Abstractpub_date
2015-09-01 00:00:00pages
477-85issue
3eissn
1389-9333issn
1573-6814journal_volume
16pub_type
杂志文章abstract：:In this study, the efficiency of the "Needle Immersed Vitrification" technique was tested on cryopreserved feline ovarian tissue. For vitrification, ovarian fragments (0.5-1.5 mm2) from each ovary were collected; the grafts were exposed to 7.5-15% ethylene glycol and 7.5-15% dimethyl sulfoxide at room temperature and ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9663-0
更新日期：2018-03-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) are used for tissue regeneration in several pathological conditions, including autoimmune diseases. However, the optimal sources and culture requirements for these cells are still under investigation. Here, we compared mRNA expression in dermal MSCs (DMSCs) at passage (P) 3 and P5 to prov...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9672-z
更新日期：2018-09-01 00:00:00
abstract：:Human epidermal melanocytes can be induced to form melanocyte spheroids and revert to immature characteristics by long-term trypsinization (LTT). To further explore the biological characteristics of melanocytes after LTT and to study the underlying mechanism. Melanocytes were subjected to long-term (2 h) trypsinizatio...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09866-9
更新日期：2020-09-25 00:00:00
abstract：:The aim of the present study was to compare the effects of two freezing methods, vapor phase and very rapid freezing, with and without cryoprotectant on semen parameters in men with normal semen criteria. Cryopreservation was done on semen samples from 31 men by two methods of vapor phase freezing and very rapid freez...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-012-9351-z
更新日期：2013-12-01 00:00:00
abstract：:Malaria is one of the exclusion criteria used in selecting tissue donors and the absence of this information can lead to rejection of tissues for transplant. The studies on the malaria parasite have been confined to low dose attenuation of parasites in blood for transfusion purposes. There is no published information ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-018-9683-4
更新日期：2018-06-01 00:00:00
abstract：:Whilst some of the assays used for serological screening of post-mortem blood samples from deceased tissue donors in some countries have been specifically validated by the manufacturer for this purpose, a significant number of those currently in use globally have not. Although specificity has previously been considere...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-010-9175-7
更新日期：2011-05-01 00:00:00
abstract：:To compare the quality of the repair tissue in three-dimensional co-culture of human chondrocytes implanted in an in vivo model. Six cadaveric and five live human donors were included. Osteochondral biopsies from the donor knees were harvested for chondrocyte isolation. Fifty percent of cadaveric chondrocytes were exp...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9635-4
更新日期：2017-09-01 00:00:00
abstract：BACKGROUND:The role of bone and tissue banking is well known to meet the ever-growing need of bone and soft tissue allografts. Strict guidelines have been established to ensure high standard and minimize complications related to bone transplantation. METHODS:The Bone Bank in King Faisal Specialist Hospital and Researc...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09868-7
更新日期：2020-10-04 00:00:00
abstract：:The purpose of this study is to evaluate the reliability of cartilage digestion and fluorescein diacetate-ethidium bromide (FDA-EB) fluorescence staining for the detection of chondrocyte viability in osteochondral grafts. Sixteen fresh osteochondral grafts were harvested from pig knee condyles, and the articular carti...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-018-9685-2
更新日期：2018-09-01 00:00:00
abstract：:Living tissue engineering for regenerative therapy cannot withstand the usual pharmacopoeia methods of purification and terminal sterilization. Consequently, these products must be manufactured under aseptic conditions at microbiologically controlled environment facilities. This study was proposed to validate BacT/ALE...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9281-1
更新日期：2012-08-01 00:00:00
abstract：:Repair of damaged cartilage usually requires replacement tissue or substitute material. Tissue engineering is a promising means to produce replacement cartilage from autologous or allogeneic cell sources. Scaffolds provide a three-dimensional (3D) structure that is essential for chondrocyte function and synthesis of c...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-005-5810-0
更新日期：2005-01-01 00:00:00
abstract：:Allogeneic mandibular bone processed by the deep frozen method was used as a biologic crib for mandibular reconstruction. The allogeneic mandible is biocompatible, bioresorbable, of low antigenicity and provides the morphology for symphysis contour, angle of mandible and dental arch form. The particulate cancellous bo...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1023602717328
更新日期：2002-01-01 00:00:00
abstract：:We describe a rapid and easy technique for the preparation of pre-stripped Descemet membrane (DM) endothelial keratoplasty (DMEK) tissue in the eye bank, without the use of dye or trephining by the eye bank technician. After drawing 2 radial marks 90° apart on the scleral rim of the cornea, a circumferential incision ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-019-09771-w
更新日期：2019-06-01 00:00:00
abstract：:On March 19 World Health Organization declare the pandemic situation by outbreak coronavirus disease 2019 in the world. The pressure on the health care system has been very high in several countries. Spanish National Transplant Organization (ONT) have made many efforts in maintaining transplantation activity. Although...
journal_title：Cell and tissue banking
pub_type： 杂志文章,多中心研究
doi：10.1007/s10561-020-09853-0
更新日期：2020-12-01 00:00:00
abstract：BACKGROUND:Despite improved surgical techniques and the use of new medication, healing of corneal and conjunctival defects cannot always be achieved. In this connection the clinical use of human amnion, produced by different techniques, has represented a successful alternative for the past ten years. The purpose of the...
journal_title：Cell and tissue banking
pub_type： 临床试验,杂志文章
doi：10.1023/b:catb.0000022222.41304.de
更新日期：2004-01-01 00:00:00
abstract：:Most protocols to cryopreserve ovarian tissue utilize the permeable cryoprotective agents (CPAs) in 1.5 M concentration. However the issues related to the ability to use higher concentrations of CPAs have remained open. The research aim was to assess the efficiency of media containing osmotically active sugars (sucros...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9623-8
更新日期：2018-03-01 00:00:00
abstract：:Although allografts for anterior cruciate ligament (ACL) replacement have shown advantages compared to autografts, their use is limited due to the risk of disease transmission and the limitations of available sterilization methods. Gamma sterilization has shown detrimental effects on graft properties at the high doses...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9289-6
更新日期：2012-08-01 00:00:00
abstract：:In order to solve some of the problems that are affecting tissue banking activities in the world, a new program/project proposal could be prepared by the IAEA and interested Member States in order to implement it in 2009. The main objective of the new program/project proposal could be the following: To consolidate tis...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-008-9092-1
更新日期：2009-05-01 00:00:00
abstract：:Musculo-skeletal allografts sterilized and deep frozen are among the most common human tissue to be preserved and utilized in modern medicine. The effects of a long deep freezing period on cortical bone has already been evaluated and found to be insignificant. However, there are no reports about the influences of a pr...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-008-9106-z
更新日期：2009-02-01 00:00:00
abstract：:Our Department of Plastic and Reconstructive Surgery has routinely been using amnion preserved in glycerol for the treatment of debrided II degrees burns. This treatment is almost pain free and requires fewer changes of dressings and fewer anaesthetics. It also prevents overgrowing granulation tissue and lessens scarr...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1014316232009
更新日期：2001-01-01 00:00:00
abstract：:A tympano-ossicular tissue bank complying with European Union regulations on human allografts is feasible and critical to assure that the patient receives tissue which is safe, individually checked and prepared in a suitable environment. The transcranial procurement technique has become the standard approach to procur...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-012-9344-y
更新日期：2013-09-01 00:00:00
abstract：:The goal of periodontal therapy is to prevent periodontal disease progression and to regenerate lost periodontal tissues. Various bone grafts are used alone or in combination with other treatment modalities for periodontal therapy. In literature no reports are presented with block allografts in the treatment of intrab...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-018-09744-5
更新日期：2019-02-11 00:00:00
abstract：:ISBT 128 is the global standard for the identification, labeling and information transfer of human blood, cell, tissue and organ products across international borders and disparate health care systems. The system has been designed and perfected over almost two decades to ensure accuracy, safety and efficiency for the ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-010-9196-2
更新日期：2010-11-01 00:00:00
abstract：:During bone allograft processing, despite stringent donor screening and use of aseptic techniques, microbial invasion may occur due to the porous nature of the graft and cause potentially fatal infections. The aim of the present study was to prepare bone allograft with and without gentamicin and to compare bioburden a...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-019-09763-w
更新日期：2019-06-01 00:00:00
abstract：:The aim of this paper was to evaluate through histological analysis of the tissue reaction of deproteinized bovine bone matrix (DBBM) when inserted into the site of intramuscular ectopic sheep. In this study, 16 sheep received 3 groups and these were divided into 2 experimental times: Group 1-sham group, Group 2-parti...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-016-9588-z
更新日期：2016-12-01 00:00:00
abstract：:In order to guarantee the required level of quality for our Bone &Tissue Banking, we evaluated a new CE marked container (CELLFLEX MacoPharma), for packing, transport, processing and storage of bones for therapeutic use. The use of CE marked containers is mandatory for organ and tissue containers (Medical Device Direc...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-004-1090-3
更新日期：2004-01-01 00:00:00
abstract：:Increasingly dental surgeons face the challenge of reconstruction of the height and/or thickness of the alveolar ridge as more and more patients wish to have permanent restoration of their dental defects based on intraosseous implants. Evaluation of human allogeneic bone tissue grafts in reconstruction of atrophied al...
journal_title：Cell and tissue banking
pub_type： 临床试验,杂志文章
doi：10.1007/s10561-015-9512-y
更新日期：2015-12-01 00:00:00
abstract：:Cryopreserved (CryoPA) and Glycerol-preserved (GPA) skin allografts are commonly used in the treatment of severe burn injuries. However, comparable data on their differences in clinical outcome is scarce. This retrospective review aims to study the effect of allograft viability on clinical outcomes. The records of 48 ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9254-4
更新日期：2012-06-01 00:00:00
abstract：:In India, the procurement of tissues for transplantation is governed by the Transplantation of Human Organs Act, 1994. However, although this law exists, it is primarily applied to organ transplantation and rules and regulations that are specific to tissue banking have yet to be developed.The Tata Memorial Hospital (T...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/B:CATB.0000007026.00604.97
更新日期：2003-01-01 00:00:00
abstract：:The aim of the study was to determine the relationship between the maternal age at delivery and selected properties of the cord blood stem cells. The study included 50 pregnant women aged between 18 and 38 years in which spontaneous labors or elective cesarean sections were performed. Umbilical cord blood was collecte...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9657-y
更新日期：2018-03-01 00:00:00
