Abstract：
:Mesenchymal stem cells (MSCs) are used for tissue regeneration in several pathological conditions, including autoimmune diseases. However, the optimal sources and culture requirements for these cells are still under investigation. Here, we compared mRNA expression in dermal MSCs (DMSCs) at passage (P) 3 and P5 to provide a reference for future studies related to DMSCs expansion. In normal DMSCs, the expression of three of eight genes associated with basic cellular activity were different at P5 compared to that at P3: PLCB4 and SYTL2 were upregulated by 4.30- and 6.42-fold, respectively (P < 0.05), whereas SATB2 was downregulated by 39.25-fold (P < 0.05). At the same time, genes associated with proliferation, differentiation, inflammation, and apoptosis were expressed at similar levels at P3 and P5 (P > 0.05). In contrast, in DMSCs isolated from psoriatic patients we observed differential expression of three inflammation-associated genes at P5 compared to P3; thus IL6, IL8, and CXCL6 mRNA levels were upregulated by 16.02-, 31.15-, and 15.04-fold, respectively. Our results indicate that normal and psoriatic DMSCs showed different expression patterns for genes related to inflammation and basic cell activity at P3 and P5, whereas those for genes linked to proliferation, differentiation, and apoptosis were mostly similar.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Li X,Li J,Zhao X,Wang Q,Yang X,Cheng Y,Zhou M,Wang G,Dang E,Yang X,Hou R,An P,Yin G,Zhang Kdoi
10.1007/s10561-017-9672-zsubject
Has Abstractpub_date
2018-09-01 00:00:00pages
277-285issue
3eissn
1389-9333issn
1573-6814pii
10.1007/s10561-017-9672-zjournal_volume
19pub_type
杂志文章abstract：:Human sperm cryopreservation is a common technique which is used in assisted reproductive technologies. Despite the existence of evidence supporting the production of ROS and DNA fragmentation during sperm cryopreservation, there is little and equivocal information about the cryopreservation effects on methylation of ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09828-1
更新日期：2020-09-01 00:00:00
abstract：:Despite a plethora of studies on overall attitudes related to organ donation in a health care setting, little is known as to whether or not medical students at various levels of undergraduate training have distinctive attitudes towards donation. The purpose of this study was to analyze attitudes of first- and sixth-ye...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9491-4
更新日期：2015-09-01 00:00:00
abstract：:The contamination of the transport solution used in cardiovascular allografts can occur from different sources. Risk factors associated with positive microbiological test of transport solution have not been reported previously. This study aimed to determine the risk factor for contamination of transport solution used ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-019-09754-x
更新日期：2019-03-01 00:00:00
abstract：:This is a report of a workshop held on the establishment of human research tissue banking which was held in Levi, Finland 21-24 March 2002. There were 21 participants from 7 European countries. This meeting was attended by representatives from academia, research tissue banks and from the Biotech and Pharmaceutical Ind...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1022811232250
更新日期：2002-01-01 00:00:00
abstract：:Cryopreserved (CryoPA) and Glycerol-preserved (GPA) skin allografts are commonly used in the treatment of severe burn injuries. However, comparable data on their differences in clinical outcome is scarce. This retrospective review aims to study the effect of allograft viability on clinical outcomes. The records of 48 ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9254-4
更新日期：2012-06-01 00:00:00
abstract：:To investigate the possible risk factors for failure of transplanted eye bank-prepared Descemet stripping automated endothelial keratoplasty (DSAEK) tissues. In a retrospective study between March 2011 and March 2019, all the failed DSAEK cases (131 cases) reported to the Central Eye Bank of Iran were compared with a ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09808-5
更新日期：2020-03-01 00:00:00
abstract：:Human sperm banking is an important procedure in the assisted reproductive technique centers. It entails sperm damage. The aim of this study was to investigate beneficial effect of Ceratonia siliqua (C. siliqua) supplement in freezing/thawing media on post thaw sperm parameters and sperm chromatin quality in normozoos...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-019-09779-2
更新日期：2019-09-01 00:00:00
abstract：:Recently Bulgarian Bone Marrow Donors Registry (BBMDR) has been established and since August 2005 it has been a member of Bone Marrow Donors Worldwide. Currently the number of healthy donors included in the BBMDR is relatively low. All donors included in the BBMDR are typed for HLA-A, -B, -DRB loci. Phylogenetic analy...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-007-9046-z
更新日期：2008-12-01 00:00:00
abstract：:We describe a rapid and easy technique for the preparation of pre-stripped Descemet membrane (DM) endothelial keratoplasty (DMEK) tissue in the eye bank, without the use of dye or trephining by the eye bank technician. After drawing 2 radial marks 90° apart on the scleral rim of the cornea, a circumferential incision ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-019-09771-w
更新日期：2019-06-01 00:00:00
abstract：:In Malaysia, tissue banking activities began in Universiti Sains Malaysia (USM) Tissue Bank in early 1990s. Since then a few other bone banks have been set up in other government hospitals and institutions. However, these banks are not governed by the national authority. In addition there is no requirement set by the ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-010-9188-2
更新日期：2010-11-01 00:00:00
abstract：:Injured nerves cannot regenerate on their own, and a lack of engraftable human nerves has been a major obstacle in cell-based therapies for regenerating damaged nerves. A monolayer culture approach to obtain adherent neural stem cells from human embryonic stem cells (hESC-NSCs) was established, and the greatest number...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09816-5
更新日期：2020-06-01 00:00:00
abstract：:This study was conducted to compare the quantitative and qualitative indices of the donated corneas maintained in either Optisol-GS or Eusol-C storage media. In an ante-grade single blind study, two corneas from each donor with a death to preservation time of less than 30 h and with a minimum of "an apparent good corn...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9466-5
更新日期：2015-06-01 00:00:00
abstract：:Human adipose-derived stem cells (hADSCs) have the ability to influence immune response, and hence are key cell sources for tissue repair and regeneration. In this study we explored the effect of continuous passage on the immunomodulatory properties of hADSCs to provide some advises for large-scale production of hADSC...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9451-z
更新日期：2015-03-01 00:00:00
abstract：:In order to solve some of the problems that are affecting tissue banking activities in the world, a new program/project proposal could be prepared by the IAEA and interested Member States in order to implement it in 2009. The main objective of the new program/project proposal could be the following: To consolidate tis...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-008-9092-1
更新日期：2009-05-01 00:00:00
abstract：:Cell manufacturing for clinical applications is a unique form of biologics manufacturing that relies on maintenance of stringent work practices designed to ensure product consistency and prevent contamination by microorganisms or by another patient's cells. More extensive, prolonged laboratory processes involve greate...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-011-9276-y
更新日期：2012-12-01 00:00:00
abstract：:Defatting is an important procedure for the preparation of bone grafts because lipids in bone grafts strongly influence the osteointegration. Lipases have been widely used in different fields. However, study on the application to defatting process for bone grafts preparation has never been found so far. In this study,...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-013-9391-z
更新日期：2014-09-01 00:00:00
abstract：:In this study, the efficiency of the "Needle Immersed Vitrification" technique was tested on cryopreserved feline ovarian tissue. For vitrification, ovarian fragments (0.5-1.5 mm2) from each ovary were collected; the grafts were exposed to 7.5-15% ethylene glycol and 7.5-15% dimethyl sulfoxide at room temperature and ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9663-0
更新日期：2018-03-01 00:00:00
abstract：:The amnionic membrane is a rich source of multipotent mesenchymal stromal cells (hAMSC), which are readily available and show a potential use in regenerative medicine and tissue engineering. Before these cells can be applied clinically, careful characterization is necessary, especially as primary cells are known to ch...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-013-9415-8
更新日期：2014-06-01 00:00:00
abstract：:The aim of this study is to evaluate the beneficial effect of Myoinositol (MYO) supplement in freezing media on the post thaw sperm quality. Semen samples from 40 normozoospermic men were divided into two aliquots and frozen with simple or 2 mg/mL MYO supplemented freezing medium. Post thaw process including, computer...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-018-9731-0
更新日期：2019-03-01 00:00:00
abstract：:Perivascular cells are known to be ancestors of mesenchymal stem cells (MSCs) and can be obtained from heart, skin, bone marrow, eye, placenta and umbilical cord (UC). However detailed characterization of perivascular cells around the human UC vein and comparative analysis of them with MSCs haven't been done yet. In t...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9542-5
更新日期：2016-06-01 00:00:00
abstract：:Homograft or allograft tissue has been available for use as replacement for diseased valves or reconstruction of major vessels for decades. However, with respect to replacement of diseased valvular tissue the search for the ideal valve still continues. In this review we will discuss the clinical indications, surgical ...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-006-9009-9
更新日期：2006-01-01 00:00:00
abstract：:Although therapeutics derived from biological sources have been subjected to regulatory oversight for some time, the products used in transplantation procedures have historically been exempt from this oversight. These products have been viewed as being part of medical practice rather than as the result of mainstream p...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-006-9012-1
更新日期：2006-01-01 00:00:00
abstract：:Musculo-skeletal allografts sterilized and deep frozen are among the most common human tissue to be preserved and utilized in modern medicine. The effects of a long deep freezing period on cortical bone has already been evaluated and found to be insignificant. However, there are no reports about the influences of a pr...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-008-9106-z
更新日期：2009-02-01 00:00:00
abstract：:The present experiment was conducted to evaluate the effect of kinetin on ram semen quality during cold storage. Ejaculates were collected using an artificial vagina from five Qezel rams. Ejaculates which met the criteria (volume of 0.75-2 ml; minimum spermatozoa concentration of 2.5 × 109 spermatozoa/ml and forward p...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-016-9604-3
更新日期：2018-03-01 00:00:00
abstract：:The regulatory framework of tissue banking introduces a number of requirements for monitoring cleanrooms for processing tissue or cell grafts. Although a number of requirements were clearly defined, some requirements are open for interpretation. This study aims to contribute to the interpretation of GMP or GTP guideli...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-012-9355-8
更新日期：2013-12-01 00:00:00
abstract：:In breast cancer, various clinical parameters are assessed to define clinical stage and thus obtain a more accurate prognosis. However, banks of tumor tissues are an important source of material for studies of risk of recurrence and of features governing clinical outcome in breast cancer. Although the heterogeneous ch...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-009-9124-5
更新日期：2009-11-01 00:00:00
abstract：:Islet transplantation has made major progress to treat patients with type 1 diabetes. Islet mass and quality are critically important to ensure successful transplantation. Currently, islet status is evaluated using insulin secretion, oxygen consumption rate, or adenosine triphosphate (ATP) measurement. These parameter...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9659-9
更新日期：2018-03-01 00:00:00
abstract：:Allogeneic mandibular bone processed by the deep frozen method was used as a biologic crib for mandibular reconstruction. The allogeneic mandible is biocompatible, bioresorbable, of low antigenicity and provides the morphology for symphysis contour, angle of mandible and dental arch form. The particulate cancellous bo...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1023602717328
更新日期：2002-01-01 00:00:00
abstract：:This study aimed to optimise methods of cryopreserving human articular cartilage (AC) tissue for the isolation of late chondrocytes. Human AC specimens from osteoarthritis patients who had undergone total knee replacement were used to optimise the chondrocyte isolation process and the choice of cryoprotective agent (C...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-012-9328-y
更新日期：2013-06-01 00:00:00
abstract：:Both allogenic and autologous cultured skin cells have been used clinically on burn patients. In vitro cultivation of human keratinocytes has been routinely provided by the Central Tissue Bank in Bratislava since 1996, with an average annual production of around 7,000 cm(2). Keratinocytes have been cultivated using a ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9275-z
更新日期：2012-08-01 00:00:00
