Abstract：
:Both allogenic and autologous cultured skin cells have been used clinically on burn patients. In vitro cultivation of human keratinocytes has been routinely provided by the Central Tissue Bank in Bratislava since 1996, with an average annual production of around 7,000 cm(2). Keratinocytes have been cultivated using a version of the original by Rheinwald and Green (Cell 6:317-330, 1975) methodology which has been modified over time in our laboratory as we gained more experience with this serial passage system. We have observed that the growth of cultured keratinocytes depends on several important factors, including the timing of skin sample procurement, the method of skin sample procurement, the general condition of the patient, the quality and composition of the culture media and, to a lesser extent, the age of the patient. We aim to share our experience with other cell cultivation facilities.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Dragúňová J,Kabát P,Koller J,Jarabinská Vdoi
10.1007/s10561-011-9275-zsubject
Has Abstractpub_date
2012-08-01 00:00:00pages
471-8issue
3eissn
1389-9333issn
1573-6814journal_volume
13pub_type
杂志文章abstract：:It has been predicted that one of the greatest increase in prevalence of diabetes will happen in the Middle East bear in the next decades. The aim of standard therapeutic strategies for diabetes is better control of complications. In contrast, some new strategies like cell and gene therapy have aimed to cure the disea...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9273-1
更新日期：2012-12-01 00:00:00
abstract：:In breast cancer, various clinical parameters are assessed to define clinical stage and thus obtain a more accurate prognosis. However, banks of tumor tissues are an important source of material for studies of risk of recurrence and of features governing clinical outcome in breast cancer. Although the heterogeneous ch...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-009-9124-5
更新日期：2009-11-01 00:00:00
abstract：:Musculo-skeletal allografts sterilized and deep frozen are among the most common human tissue to be preserved and utilized in modern medicine. The effects of a long deep freezing period on cortical bone has already been evaluated and found to be insignificant. However, there are no reports about the influences of a pr...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-008-9106-z
更新日期：2009-02-01 00:00:00
abstract：:Recently Bulgarian Bone Marrow Donors Registry (BBMDR) has been established and since August 2005 it has been a member of Bone Marrow Donors Worldwide. Currently the number of healthy donors included in the BBMDR is relatively low. All donors included in the BBMDR are typed for HLA-A, -B, -DRB loci. Phylogenetic analy...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-007-9046-z
更新日期：2008-12-01 00:00:00
abstract：:Autologous chondrocyte implantation has been shown to be a promising method for treatment of deep articular cartilage defects. The hyaline cartilage formed by implanted autologous chondrocytes has biomechanical properties similar to those of natural articular cartilage. Between June 2006 and September 2008 we performe...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-009-9163-y
更新日期：2010-05-01 00:00:00
abstract：:Revision surgery of joint replacements is increasing and raises the demand for allograft bone since restoration of bone stock is crucial for longevity of implants. Proceedings of bone grafts influence the biological and mechanic properties differently. This study examines the effect of thermodisinfection on mechanic p...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-016-9567-4
更新日期：2016-09-01 00:00:00
abstract：:Nowadays, there are four types of meniscal allografts known: fresh, cryopreserved, deep-frozen and lyophilized ones but only two of them are widely used in clinical practice. Use of different types of meniscal allografts still remains controversial due to preparation method, their biomechanical properties as well as c...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-013-9396-7
更新日期：2014-09-01 00:00:00
abstract：:Increasingly dental surgeons face the challenge of reconstruction of the height and/or thickness of the alveolar ridge as more and more patients wish to have permanent restoration of their dental defects based on intraosseous implants. Evaluation of human allogeneic bone tissue grafts in reconstruction of atrophied al...
journal_title：Cell and tissue banking
pub_type： 临床试验,杂志文章
doi：10.1007/s10561-015-9512-y
更新日期：2015-12-01 00:00:00
abstract：:National Blood Service (NBS) Tissue Services (TS) operates living donor and deceased donor tissue banking programmes. The living bone donor programme operates in collaboration with 91 orthopaedic departments across the country and collects bone donations, in the form of surgically removed femoral heads (FHs), from ove...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-005-2362-2
更新日期：2006-01-01 00:00:00
abstract：:The aim of the study was to determine the relationship between the maternal age at delivery and selected properties of the cord blood stem cells. The study included 50 pregnant women aged between 18 and 38 years in which spontaneous labors or elective cesarean sections were performed. Umbilical cord blood was collecte...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9657-y
更新日期：2018-03-01 00:00:00
abstract：:Perivascular cells are known to be ancestors of mesenchymal stem cells (MSCs) and can be obtained from heart, skin, bone marrow, eye, placenta and umbilical cord (UC). However detailed characterization of perivascular cells around the human UC vein and comparative analysis of them with MSCs haven't been done yet. In t...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9542-5
更新日期：2016-06-01 00:00:00
abstract：:Delivering the promise of personalised medicine is the challenge that the current generation of scientists face. The variations in human physiology and disease are considerable, and designing appropriate strategies to deliver what has been promised will require access to tissue from a large number of volunteers. The N...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-010-9212-6
更新日期：2011-02-01 00:00:00
abstract：:Most protocols to cryopreserve ovarian tissue utilize the permeable cryoprotective agents (CPAs) in 1.5 M concentration. However the issues related to the ability to use higher concentrations of CPAs have remained open. The research aim was to assess the efficiency of media containing osmotically active sugars (sucros...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9623-8
更新日期：2018-03-01 00:00:00
abstract：:The use of glycosaminoglycans (GAGs) as the second macromolecular component with collagen for the membrane preparation is based on the idea to create a biodegradable scaffold for new tissue. A hybrid collagen/hyaluronan membrane with specific bubble macrostructure was designed to serve as a synthetic dermal substitute...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/a:1010181925236
更新日期：2000-01-01 00:00:00
abstract：:Stem cell therapy is in constant need of new cell sources to conceive regenerative medicine approaches for diseases that are still without therapy. Scientists drew the attention toward amniotic membrane and amniotic fluid stem cells, since these sources possess many advantages: first of all as cells can be extracted f...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-014-9428-y
更新日期：2014-06-01 00:00:00
abstract：:Human skin allografts can be preserved by different methods. In our clinical practice, human skin allografts are harvested on multi-organ and tissue donors, transferred at +4°C in Ringer Lactate, cryopreserved with 15% Glycerol and held in the vapor phase of a liquid nitrogen freezer until delivery to the burn center....
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9239-3
更新日期：2012-03-01 00:00:00
abstract：:Whilst some of the assays used for serological screening of post-mortem blood samples from deceased tissue donors in some countries have been specifically validated by the manufacturer for this purpose, a significant number of those currently in use globally have not. Although specificity has previously been considere...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-010-9175-7
更新日期：2011-05-01 00:00:00
abstract：:This study investigated cryopreserved pulmonary homograft (CPA) structural integrity after prolonged cold ischemic harvesting times in a juvenile sheep model. Three groups with different post-mortem cold ischemic harvesting times were studied, i.e. Group 1 (24 h, n = 10); group 2 (48 h, n = 10); group 3 (72 h, n = 10)...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9500-2
更新日期：2015-12-01 00:00:00
abstract：:The Central Tissue Bank in Warsaw was established in 1963 and since then ionising radiation has been routinely applied to sterilise tissue grafts. Connective tissue grafts such as bone, cartilage, tendons, sclera, pericardium, skin, acellular dermis and amnion irradiated with a dose of 35 kGy in a (60)Co source and/or...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-005-0338-x
更新日期：2005-01-01 00:00:00
abstract：:Although therapeutics derived from biological sources have been subjected to regulatory oversight for some time, the products used in transplantation procedures have historically been exempt from this oversight. These products have been viewed as being part of medical practice rather than as the result of mainstream p...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-006-9012-1
更新日期：2006-01-01 00:00:00
abstract：:Homograft or allograft tissue has been available for use as replacement for diseased valves or reconstruction of major vessels for decades. However, with respect to replacement of diseased valvular tissue the search for the ideal valve still continues. In this review we will discuss the clinical indications, surgical ...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-006-9009-9
更新日期：2006-01-01 00:00:00
abstract：:The present experiment was conducted to evaluate the effect of kinetin on ram semen quality during cold storage. Ejaculates were collected using an artificial vagina from five Qezel rams. Ejaculates which met the criteria (volume of 0.75-2 ml; minimum spermatozoa concentration of 2.5 × 109 spermatozoa/ml and forward p...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-016-9604-3
更新日期：2018-03-01 00:00:00
abstract：:We evaluated the value of the 'alternative slices mirror image method' used in prostate tissue banking in terms of predicting the sampling of cancerous tissue while preserving the pathological prognostic information. The concordance of diagnosis between banked sections and their mirror image paraffin- sections was stu...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9284-y
更新日期：2012-12-01 00:00:00
abstract：:Cryopreserved (CryoPA) and Glycerol-preserved (GPA) skin allografts are commonly used in the treatment of severe burn injuries. However, comparable data on their differences in clinical outcome is scarce. This retrospective review aims to study the effect of allograft viability on clinical outcomes. The records of 48 ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9254-4
更新日期：2012-06-01 00:00:00
abstract：:Current regulatory requirements demand an in-depth understanding and validation of protocols used in tissue banking. The aim of this work was to characterize the quality of split thickness skin allografts cryopreserved or manufactured using highly concentrated solutions of glycerol (50, 85 or 98%), where tissue water ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-018-9694-1
更新日期：2018-06-01 00:00:00
abstract：:Osteochondral defects can degenerate into osteoarthritis and currently there are no good treatment alternatives available to most Orthopaedic surgeons. Osteochondral allografting can restore damaged joint surfaces but its clinical use is limited by poor access to high quality tissue. Vitrification of osteochondral tis...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-006-9023-y
更新日期：2007-01-01 00:00:00
abstract：:Human skin allografts are one of the best temporary biological coverings for severely burned patients. Cryopreserved (CPA) and glycerol-preserved (GPA) allografts are the most widely used types. This study compared the allograft efficiency of both preservation methods under the same conditions. To simulate actual clin...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9521-x
更新日期：2016-03-01 00:00:00
abstract：:Although allografts for anterior cruciate ligament (ACL) replacement have shown advantages compared to autografts, their use is limited due to the risk of disease transmission and the limitations of available sterilization methods. Gamma sterilization has shown detrimental effects on graft properties at the high doses...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9289-6
更新日期：2012-08-01 00:00:00
abstract：:To investigate the possible risk factors for failure of transplanted eye bank-prepared Descemet stripping automated endothelial keratoplasty (DSAEK) tissues. In a retrospective study between March 2011 and March 2019, all the failed DSAEK cases (131 cases) reported to the Central Eye Bank of Iran were compared with a ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09808-5
更新日期：2020-03-01 00:00:00
abstract：:Appropriate animal models are critical to conduct translational studies of human disorders without variables that can confound clinical studies. Such analytic methods as patch-clamp electrophysiological and voltammetric recordings of neurons in brain slices require living brain tissue. In order to obtain viable tissue...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-013-9380-2
更新日期：2014-03-01 00:00:00
