Dimethyl sulfoxide toxicity kinetics in intact articular cartilage.

Abstract:

:Osteochondral defects can degenerate into osteoarthritis and currently there are no good treatment alternatives available to most Orthopaedic surgeons. Osteochondral allografting can restore damaged joint surfaces but its clinical use is limited by poor access to high quality tissue. Vitrification of osteochondral tissue would allow the banking of this tissue but requires high concentrations of cryoprotective agents. This study was designed to ascertain dimethyl sulfoxide (DMSO) toxicity kinetics to chondrocytes in situ after exposure to DMSO at different temperatures recorded as a function of time. Porcine osteochondral dowels were exposed to 1, 3, 5, and 6M DMSO at 4, 22, and 37 degrees C for 0.5 min to 120 min. Chondrocyte recovery was determined by membrane integrity (Syto 13 and ethidium bromide) and mitochondrial (WST-1) assays. Results demonstrated that cell recovery was concentration, temperature and time dependent. At higher concentrations and temperatures, significant cell loss occurred within minutes. A rate constant calculated for chondrocyte death was dependent on temperature. 1 M DMSO appeared relatively non-toxic. This experiment established a method to examine systematically toxicity parameters for chondrocytes in situ and this data can be used to tailor vitrification protocols by limiting exposure temperature and time or lowering DMSO concentrations below toxic levels recorded.

journal_name

Cell Tissue Bank

journal_title

Cell and tissue banking

authors

Elmoazzen HY,Poovadan A,Law GK,Elliott JA,McGann LE,Jomha NM

doi

10.1007/s10561-006-9023-y

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

125-33

issue

2

eissn

1389-9333

issn

1573-6814

journal_volume

8

pub_type

杂志文章
  • Analysis of impact on tissue activity during COVID-19 outbreak: a survey of 8 banks in Spain.

    abstract::On March 19 World Health Organization declare the pandemic situation by outbreak coronavirus disease 2019 in the world. The pressure on the health care system has been very high in several countries. Spanish National Transplant Organization (ONT) have made many efforts in maintaining transplantation activity. Although...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章,多中心研究

    doi:10.1007/s10561-020-09853-0

    authors: Villalba R,Santos S,Martinez MJ,Díaz M,Pevida M,Cemborain A,Casares C,Mirabet V

    更新日期:2020-12-01 00:00:00

  • A banking strategy toward customized therapy in breast cancer.

    abstract::In breast cancer, various clinical parameters are assessed to define clinical stage and thus obtain a more accurate prognosis. However, banks of tumor tissues are an important source of material for studies of risk of recurrence and of features governing clinical outcome in breast cancer. Although the heterogeneous ch...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-009-9124-5

    authors: Nardone A,Cavaliere C,Corvigno S,Limite G,De Placido S,Veneziani BM

    更新日期:2009-11-01 00:00:00

  • Activation of caspases in human spermatozoa during cryopreservation--an immunoblot study.

    abstract::Cellular stress to ejaculated spermatozoa such as cryopreservation is known to induce caspase-derived, apoptotic signaling. Therefore, the proenzymes and active forms of caspases 1, 3, 8 and 9 were examined by western blot technique in unfrozen and frozen human spermatozoa of infertility patients and of healthy donors...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-005-0276-7

    authors: Wündrich K,Paasch U,Leicht M,Glander HJ

    更新日期:2006-01-01 00:00:00

  • Chondrocyte suspension in fibrin glue.

    abstract::Autologous chondrocyte implantation has been shown to be a promising method for treatment of deep articular cartilage defects. The hyaline cartilage formed by implanted autologous chondrocytes has biomechanical properties similar to those of natural articular cartilage. Between June 2006 and September 2008 we performe...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-009-9163-y

    authors: Wysocka A,Mann K,Bursig H,Dec J,Gaździk TS

    更新日期:2010-05-01 00:00:00

  • Are heart valves from donors over 65 years of age morphologically suitable for transplantation?

    abstract::Since there is no upper age limit for general organ donation, unlike heart valve donation, and since a quarter of all organ donors are 65 years and older, we examined whether the heart valves from these donors are suitable as allografts. In the period 1999-2004 the aortic valve and pulmonary valve of 100 organ donors ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-007-9052-1

    authors: Grosse K,Meyer R,Schmitzer E,Hetzer R,Wesslau C

    更新日期:2008-03-01 00:00:00

  • Donor exclusion in the National Blood Service Tissue Services living bone donor programme.

    abstract::National Blood Service (NBS) Tissue Services (TS) operates living donor and deceased donor tissue banking programmes. The living bone donor programme operates in collaboration with 91 orthopaedic departments across the country and collects bone donations, in the form of surgically removed femoral heads (FHs), from ove...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-005-2362-2

    authors: Pink F,Warwick RM,Purkis J,Pearson J

    更新日期:2006-01-01 00:00:00

  • The role of IL-6 in bone marrow (BM)-derived mesenchymal stem cells (MSCs) proliferation and chondrogenesis.

    abstract::Rheumatoid arthritis (RA) is the most common degenerative arthritic cartilage and represents a disease where the prospect of stem cell therapy offers considerable hope. Currently, bone marrow (BM) represents the major source of mesenchymal stem cells (MSCs) for cell therapy. In the pathology of RA, the pro-inflammator...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-012-9354-9

    authors: Wei H,Shen G,Deng X,Lou D,Sun B,Wu H,Long L,Ding T,Zhao J

    更新日期:2013-12-01 00:00:00

  • Amnion-derived mesenchymal stromal cells show a mesenchymal-epithelial phenotype in culture.

    abstract::The amnionic membrane is a rich source of multipotent mesenchymal stromal cells (hAMSC), which are readily available and show a potential use in regenerative medicine and tissue engineering. Before these cells can be applied clinically, careful characterization is necessary, especially as primary cells are known to ch...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-013-9415-8

    authors: König J,Lang I,Siwetz M,Fröhlich J,Huppertz B

    更新日期:2014-06-01 00:00:00

  • Isolation of human dermis derived mesenchymal stem cells using explants culture method: expansion and phenotypical characterization.

    abstract::Recent studies have reported that stem cells can be isolated from a wide range of tissues including bone marrow, fatty tissue, adipose tissue and placenta. Moreover, several studies also suggest that skin dermis could serve as a source of stem cells, but are of unclear phenotype. Therefore, we isolated and investigate...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-014-9471-8

    authors: Park JR,Kim E,Yang J,Lee H,Hong SH,Woo HM,Park SM,Na S,Yang SR

    更新日期:2015-06-01 00:00:00

  • Physical changes of amniotic membranes through glycerolization for the use as an epidermal substitute. Light and electron microscopic studies.

    abstract::Our Department of Plastic and Reconstructive Surgery has routinely been using amnion preserved in glycerol for the treatment of debrided II degrees burns. This treatment is almost pain free and requires fewer changes of dressings and fewer anaesthetics. It also prevents overgrowing granulation tissue and lessens scarr...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1023/A:1014316232009

    authors: Rejzek A,Weyer F,Eichberger R,Gebhart W

    更新日期:2001-01-01 00:00:00

  • Intact human amniotic membrane differentiated towards the chondrogenic lineage.

    abstract::Human amniotic membrane (hAM) represents a tissue that is well established as biomaterial in the clinics with potential for new applications in regenerative medicine. For tissue engineering (TE) strategies, cells are usually combined with inductive factors and a carrier substrate. We have previously recognized that hA...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-014-9454-9

    authors: Lindenmair A,Nürnberger S,Stadler G,Meinl A,Hackl C,Eibl J,Gabriel C,Hennerbichler S,Redl H,Wolbank S

    更新日期:2014-06-01 00:00:00

  • Combination of retinoic acid, dimethyl sulfoxide and 5-azacytidine promotes cardiac differentiation of human fetal liver-derived mesenchymal stem cells.

    abstract::There are controversial reports about cardiac differentiation potential of mesenchymal stem cells (MSCs), and there is still no well-defined protocol for the induction of cardiac differentiation. The effects of retinoic acid (RA) and dimethyl sulfoxide (DMSO) on the proliferation and differentiation of human fetal liv...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-015-9514-9

    authors: Deng F,Lei H,Hu Y,He L,Fu H,Feng R,Feng P,Huang W,Wang X,Chang J

    更新日期:2016-03-01 00:00:00

  • Comparative study of DFDBA and FDBA block grafts in combination with chorion membrane for the treatment of periodontal intra-bony defects at 12 months post surgery.

    abstract::The goal of periodontal therapy is to prevent periodontal disease progression and to regenerate lost periodontal tissues. Various bone grafts are used alone or in combination with other treatment modalities for periodontal therapy. In literature no reports are presented with block allografts in the treatment of intrab...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-018-09744-5

    authors: Kothiwale S,Bhimani R,Kaderi M,Ajbani J

    更新日期:2019-02-11 00:00:00

  • Does supplementation of sperm freezing/thawing media with Ceratonia siliqua improve detrimental effect of cryopreservation on sperm parameters and chromatin quality in normozoospermic specimens?

    abstract::Human sperm banking is an important procedure in the assisted reproductive technique centers. It entails sperm damage. The aim of this study was to investigate beneficial effect of Ceratonia siliqua (C. siliqua) supplement in freezing/thawing media on post thaw sperm parameters and sperm chromatin quality in normozoos...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-019-09779-2

    authors: Faramarzi A,Aghaz F,Golestan Jahromi M,Bakhtiari M,Khazaei M

    更新日期:2019-09-01 00:00:00

  • Experience gained during the long term cultivation of keratinocytes for treatment of burns patients.

    abstract::Both allogenic and autologous cultured skin cells have been used clinically on burn patients. In vitro cultivation of human keratinocytes has been routinely provided by the Central Tissue Bank in Bratislava since 1996, with an average annual production of around 7,000 cm(2). Keratinocytes have been cultivated using a ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-011-9275-z

    authors: Dragúňová J,Kabát P,Koller J,Jarabinská V

    更新日期:2012-08-01 00:00:00

  • Qualification of serological infectious disease assays for the screening of samples from deceased tissue donors.

    abstract::Whilst some of the assays used for serological screening of post-mortem blood samples from deceased tissue donors in some countries have been specifically validated by the manufacturer for this purpose, a significant number of those currently in use globally have not. Although specificity has previously been considere...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-010-9175-7

    authors: Kitchen AD,Newham JA

    更新日期:2011-05-01 00:00:00

  • Banking of fresh-frozen prostate tissue using the alternate mirror image protocol: methods, validation, and impact on the pathological prognostic parameters in radical prostatectomy.

    abstract::We evaluated the value of the 'alternative slices mirror image method' used in prostate tissue banking in terms of predicting the sampling of cancerous tissue while preserving the pathological prognostic information. The concordance of diagnosis between banked sections and their mirror image paraffin- sections was stu...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-011-9284-y

    authors: Brimo F,Sircar K,Chevalier S,Saad F,Lacombe L,Têtu B,Scarlata E,Aprikian A

    更新日期:2012-12-01 00:00:00

  • Assessment of bioburden on human and animal tissues: Part 1--results of method development and validation studies.

    abstract::Recovered human and animal tissues are used extensively in surgery for wound repair and reconstruction. In preparation for the validation of chemical disinfection and radiation sterilization processes, studies were performed on the development and validation of quantitative bioburden recovery methods for human bone an...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-010-9238-9

    authors: Kowalski JB,Mosley GA,Merritt K,Osborne J

    更新日期:2012-03-01 00:00:00

  • Co-culture of dedifferentiated and primary human chondrocytes obtained from cadaveric donor enhance the histological quality of repair tissue: an in-vivo animal study.

    abstract::To compare the quality of the repair tissue in three-dimensional co-culture of human chondrocytes implanted in an in vivo model. Six cadaveric and five live human donors were included. Osteochondral biopsies from the donor knees were harvested for chondrocyte isolation. Fifty percent of cadaveric chondrocytes were exp...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-017-9635-4

    authors: Olivos-Meza A,Velasquillo Martínez C,Olivos Díaz B,Landa-Solís C,Brittberg M,Pichardo Bahena R,Ortega Sanchez C,Martínez V,Alvarez Lara E,Ibarra-Ponce de León JC

    更新日期:2017-09-01 00:00:00

  • Trephine- and dye-free technique for eye bank preparation of pre-stripped Descemet membrane endothelial keratoplasty tissue.

    abstract::We describe a rapid and easy technique for the preparation of pre-stripped Descemet membrane (DM) endothelial keratoplasty (DMEK) tissue in the eye bank, without the use of dye or trephining by the eye bank technician. After drawing 2 radial marks 90° apart on the scleral rim of the cornea, a circumferential incision ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-019-09771-w

    authors: Chamani T,Javadi MA,Kanavi MR

    更新日期:2019-06-01 00:00:00

  • The establishment of a network of European human research tissue banks.

    abstract::This is a report of a workshop held on the establishment of human research tissue banking which was held in Levi, Finland 21-24 March 2002. There were 21 participants from 7 European countries. This meeting was attended by representatives from academia, research tissue banks and from the Biotech and Pharmaceutical Ind...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1023/A:1022811232250

    authors: Orr S,Alexandre E,Clark B,Combes R,Fels LM,Gray N,Jönsson-Rylander AC,Helin H,Koistinen J,Oinonen T,Richert L,Ravid R,Salonen J,Teesalu T,Thasler W,Trafford J,Van Der Valk J,Von Versen R,Weiss T,Womack C,Ylikomi T

    更新日期:2002-01-01 00:00:00

  • Comparison of impact of two decontamination solutions on the viability of the cells in human amnion.

    abstract::Human amniotic membrane (HAM) is used as an allograft in regenerative medicine or as a source of pluripotent cells for stem cell research. Various decontamination protocols and solutions are used to sterilize HAM before its application, but little is known about the toxicity of disinfectants on HAM cells. In this stud...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-017-9636-3

    authors: Smeringaiova I,Trosan P,Mrstinova MB,Matecha J,Burkert J,Bednar J,Jirsova K

    更新日期:2017-09-01 00:00:00

  • Encompassing ATP, DNA, insulin, and protein content for quantification and assessment of human pancreatic islets.

    abstract::Islet transplantation has made major progress to treat patients with type 1 diabetes. Islet mass and quality are critically important to ensure successful transplantation. Currently, islet status is evaluated using insulin secretion, oxygen consumption rate, or adenosine triphosphate (ATP) measurement. These parameter...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-017-9659-9

    authors: Qi M,Bilbao S,Forouhar E,Kandeel F,Al-Abdullah IH

    更新日期:2018-03-01 00:00:00

  • Defined serum- and xeno-free cryopreservation of mesenchymal stem cells.

    abstract::Mesenchymal stem cells (MSCs) have vast potential in cell therapy, and are experimentally used in the clinic. Therefore, it is critical to find a serum- and xeno-free cryopreservation method. The aim of this study was to compare two serum- and xeno-free cryoprotectants for MSCs. Adipose tissue MSCs (Ad-MSCs) and bone ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-014-9463-8

    authors: Al-Saqi SH,Saliem M,Quezada HC,Ekblad Å,Jonasson AF,Hovatta O,Götherström C

    更新日期:2015-06-01 00:00:00

  • Sporicidal efficacy of genipin: a potential theoretical alternative for biomaterial and tissue graft sterilization.

    abstract::Terminal sterilization of musculoskeletal allografts by gamma radiation minimizes the risk of disease transmission but impairs allograft mechanical properties. Commonly employed crosslinking agents can sterilize tissues without affecting mechanical properties adversely; however, these agents are toxic. Genipin is repo...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-012-9335-z

    authors: Reich MS,Akkus O

    更新日期:2013-09-01 00:00:00

  • Efficiency of mannitol-supplemented medium during adding/removing ovarian tissue with penetrating cryoprotective agents.

    abstract::Most protocols to cryopreserve ovarian tissue utilize the permeable cryoprotective agents (CPAs) in 1.5 M concentration. However the issues related to the ability to use higher concentrations of CPAs have remained open. The research aim was to assess the efficiency of media containing osmotically active sugars (sucros...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-017-9623-8

    authors: Kiroshka V,Trutaieva I,Bondarenko T

    更新日期:2018-03-01 00:00:00

  • Principles of bone and tissue banking in Saudi Arabia: 10-year experience report.

    abstract:BACKGROUND:The role of bone and tissue banking is well known to meet the ever-growing need of bone and soft tissue allografts. Strict guidelines have been established to ensure high standard and minimize complications related to bone transplantation. METHODS:The Bone Bank in King Faisal Specialist Hospital and Researc...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-020-09868-7

    authors: Ilyas I,Al-Rabiah AM,Alhussainan TS,Alrumaih HA,Fallatah AB,Alsakran SA,Al-Mohrej OA

    更新日期:2020-10-04 00:00:00

  • Reliability of cartilage digestion and FDA-EB fluorescence staining for the detection of chondrocyte viability in osteochondral grafts.

    abstract::The purpose of this study is to evaluate the reliability of cartilage digestion and fluorescein diacetate-ethidium bromide (FDA-EB) fluorescence staining for the detection of chondrocyte viability in osteochondral grafts. Sixteen fresh osteochondral grafts were harvested from pig knee condyles, and the articular carti...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-018-9685-2

    authors: Qi J,Cao F,Han Y,Xie D,Song H,Chen B,Zhou L

    更新日期:2018-09-01 00:00:00

  • Tissue banking for research: connecting the disconnected.

    abstract::Delivering the promise of personalised medicine is the challenge that the current generation of scientists face. The variations in human physiology and disease are considerable, and designing appropriate strategies to deliver what has been promised will require access to tissue from a large number of volunteers. The N...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-010-9212-6

    authors: Thomas G

    更新日期:2011-02-01 00:00:00

  • Endoscopic procurement of tympano-ossicular allografts: alternative to the transcranial or retroauricular technique.

    abstract::A tympano-ossicular tissue bank complying with European Union regulations on human allografts is feasible and critical to assure that the patient receives tissue which is safe, individually checked and prepared in a suitable environment. The transcranial procurement technique has become the standard approach to procur...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-012-9344-y

    authors: Van Rompaey V,Caremans J,Hamans E,Muylle L,Van de Heyning P

    更新日期:2013-09-01 00:00:00